The two faces of titanium dioxide nanoparticles bio-camouflage in 3D bone spheroids
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1038/s41598-019-45797-6 http://hdl.handle.net/11449/184545 |
Resumo: | Titanium (Ti) and its alloys are widely used in dental implants and hip-prostheses due to their excellent biocompatibility. Growing evidence support that surface degradation due to corrosion and wear processes, contribute to implant failure, since the release of metallic ions and wear particles generate local tissue reactions (peri-implant inflammatory reactions). The generated ions and wear debris (particles at the micron and nanoscale) stay, in a first moment, at the interface implant-bone. However, depending on their size, they can enter blood circulation possibly contributing to systemic reactions and toxicities. Most of the nanotoxicological studies with titanium dioxide nanoparticles (TiO(2)NPs) use conventional two-dimensional cell culture monolayers to explore macrophage and monocyte activation, where limited information regarding bone cells is available. Recently threedimensional models have been gaining prominence since they present a greater anatomical and physiological relevance. Taking this into consideration, in this work we developed a human osteoblastlike spheroid model, which closely mimics bone cell-cell interactions, providing a more realistic scenario for nanotoxicological studies. The treatment of spheroids with different concentrations ofTiO(2)NPs during 72 h did not change their viability significantly. Though, higher concentrations ofTiO(2)NPs influenced osteoblast cell cycle without interfering in their ability to differentiate and mineralize. For higher concentration ofTiO(2)NPs, collagen deposition and pro-inflammatory cytokine, chemokine and growth factor secretion (involved in osteolysis and bone homeostasis) increased. These results raise the possible use of this model in nanotoxicological studies of osseointegrated devices and demonstrate a possible therapeutic potential of this TiO(2)NPs to prevent or reverse bone resorption. |
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The two faces of titanium dioxide nanoparticles bio-camouflage in 3D bone spheroidsTitanium (Ti) and its alloys are widely used in dental implants and hip-prostheses due to their excellent biocompatibility. Growing evidence support that surface degradation due to corrosion and wear processes, contribute to implant failure, since the release of metallic ions and wear particles generate local tissue reactions (peri-implant inflammatory reactions). The generated ions and wear debris (particles at the micron and nanoscale) stay, in a first moment, at the interface implant-bone. However, depending on their size, they can enter blood circulation possibly contributing to systemic reactions and toxicities. Most of the nanotoxicological studies with titanium dioxide nanoparticles (TiO(2)NPs) use conventional two-dimensional cell culture monolayers to explore macrophage and monocyte activation, where limited information regarding bone cells is available. Recently threedimensional models have been gaining prominence since they present a greater anatomical and physiological relevance. Taking this into consideration, in this work we developed a human osteoblastlike spheroid model, which closely mimics bone cell-cell interactions, providing a more realistic scenario for nanotoxicological studies. The treatment of spheroids with different concentrations ofTiO(2)NPs during 72 h did not change their viability significantly. Though, higher concentrations ofTiO(2)NPs influenced osteoblast cell cycle without interfering in their ability to differentiate and mineralize. For higher concentration ofTiO(2)NPs, collagen deposition and pro-inflammatory cytokine, chemokine and growth factor secretion (involved in osteolysis and bone homeostasis) increased. These results raise the possible use of this model in nanotoxicological studies of osseointegrated devices and demonstrate a possible therapeutic potential of this TiO(2)NPs to prevent or reverse bone resorption.Rio de Janeiro cell bank (BCRJ, Rio de Janeiro, Brazil)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Propesq-Unigranrio-FUNADEP ScholarshipFundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ)Natl Inst Metrol Qual & Technol, Directory Life Sci Appl Metrol, Rio De Janeiro, BrazilNatl Inst Metrol Qual & Technol, Postgrad Program Biotechnol, Rio De Janeiro, BrazilIBTN, Brazilian Branch, Bauru, BrazilBrazilian Ctr Res Phys, Rio De Janeiro, BrazilUniv Grande Rio, Postgrad Program Translat Biomed, Duque De Caxias, BrazilUniv Fed Rio de Janeiro, Clementino Fraga Filho Univ Hosp, Inst Biomed Sci, Rio De Janeiro, BrazilNatl Inst Metrol Qual & Technol, Mat Metrol Div, Rio De Janeiro, BrazilUniv Fed Rio de Janeiro, Inst Biophys Carlos Chagas Filho, Rio De Janeiro, BrazilUniv Porto, Fac Dent Med, Porto, PortugalUniv Estadual Paulista, Phys Dept, Sao Paulo, BrazilFluminense Fed Univ, Dent Sch, Niteroi, RJ, BrazilUniv Porto, LAQV REQUIMTE, Porto, PortugalUniv Estadual Paulista, Phys Dept, Sao Paulo, BrazilCNPq: 405030/2015-0CNPq: 306672/2016-2CNPq: 467513/2014-7Nature Publishing GroupNatl Inst Metrol Qual & TechnolIBTNBrazilian Ctr Res PhysUniv Grande RioUniversidade Federal do Rio de Janeiro (UFRJ)Univ PortoUniversidade Estadual Paulista (Unesp)Fluminense Fed UnivSouza, W.Piperni, S. G.Laviola, P.Rossi, A. L.Rossi, Maria Isabel D.Archanjo, Braulio S.Leite, P. E.Fernandes, M. H.Rocha, L. A. [UNESP]Granjeiro, J. M.Ribeiro, A. R.2019-10-04T12:14:32Z2019-10-04T12:14:32Z2019-06-27info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article14http://dx.doi.org/10.1038/s41598-019-45797-6Scientific Reports. London: Nature Publishing Group, v. 9, 14 p., 2019.2045-2322http://hdl.handle.net/11449/18454510.1038/s41598-019-45797-6WOS:000472999700001Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengScientific Reportsinfo:eu-repo/semantics/openAccess2021-10-23T19:49:50Zoai:repositorio.unesp.br:11449/184545Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T19:49:50Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
The two faces of titanium dioxide nanoparticles bio-camouflage in 3D bone spheroids |
title |
The two faces of titanium dioxide nanoparticles bio-camouflage in 3D bone spheroids |
spellingShingle |
The two faces of titanium dioxide nanoparticles bio-camouflage in 3D bone spheroids Souza, W. |
title_short |
The two faces of titanium dioxide nanoparticles bio-camouflage in 3D bone spheroids |
title_full |
The two faces of titanium dioxide nanoparticles bio-camouflage in 3D bone spheroids |
title_fullStr |
The two faces of titanium dioxide nanoparticles bio-camouflage in 3D bone spheroids |
title_full_unstemmed |
The two faces of titanium dioxide nanoparticles bio-camouflage in 3D bone spheroids |
title_sort |
The two faces of titanium dioxide nanoparticles bio-camouflage in 3D bone spheroids |
author |
Souza, W. |
author_facet |
Souza, W. Piperni, S. G. Laviola, P. Rossi, A. L. Rossi, Maria Isabel D. Archanjo, Braulio S. Leite, P. E. Fernandes, M. H. Rocha, L. A. [UNESP] Granjeiro, J. M. Ribeiro, A. R. |
author_role |
author |
author2 |
Piperni, S. G. Laviola, P. Rossi, A. L. Rossi, Maria Isabel D. Archanjo, Braulio S. Leite, P. E. Fernandes, M. H. Rocha, L. A. [UNESP] Granjeiro, J. M. Ribeiro, A. R. |
author2_role |
author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Natl Inst Metrol Qual & Technol IBTN Brazilian Ctr Res Phys Univ Grande Rio Universidade Federal do Rio de Janeiro (UFRJ) Univ Porto Universidade Estadual Paulista (Unesp) Fluminense Fed Univ |
dc.contributor.author.fl_str_mv |
Souza, W. Piperni, S. G. Laviola, P. Rossi, A. L. Rossi, Maria Isabel D. Archanjo, Braulio S. Leite, P. E. Fernandes, M. H. Rocha, L. A. [UNESP] Granjeiro, J. M. Ribeiro, A. R. |
description |
Titanium (Ti) and its alloys are widely used in dental implants and hip-prostheses due to their excellent biocompatibility. Growing evidence support that surface degradation due to corrosion and wear processes, contribute to implant failure, since the release of metallic ions and wear particles generate local tissue reactions (peri-implant inflammatory reactions). The generated ions and wear debris (particles at the micron and nanoscale) stay, in a first moment, at the interface implant-bone. However, depending on their size, they can enter blood circulation possibly contributing to systemic reactions and toxicities. Most of the nanotoxicological studies with titanium dioxide nanoparticles (TiO(2)NPs) use conventional two-dimensional cell culture monolayers to explore macrophage and monocyte activation, where limited information regarding bone cells is available. Recently threedimensional models have been gaining prominence since they present a greater anatomical and physiological relevance. Taking this into consideration, in this work we developed a human osteoblastlike spheroid model, which closely mimics bone cell-cell interactions, providing a more realistic scenario for nanotoxicological studies. The treatment of spheroids with different concentrations ofTiO(2)NPs during 72 h did not change their viability significantly. Though, higher concentrations ofTiO(2)NPs influenced osteoblast cell cycle without interfering in their ability to differentiate and mineralize. For higher concentration ofTiO(2)NPs, collagen deposition and pro-inflammatory cytokine, chemokine and growth factor secretion (involved in osteolysis and bone homeostasis) increased. These results raise the possible use of this model in nanotoxicological studies of osseointegrated devices and demonstrate a possible therapeutic potential of this TiO(2)NPs to prevent or reverse bone resorption. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-04T12:14:32Z 2019-10-04T12:14:32Z 2019-06-27 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1038/s41598-019-45797-6 Scientific Reports. London: Nature Publishing Group, v. 9, 14 p., 2019. 2045-2322 http://hdl.handle.net/11449/184545 10.1038/s41598-019-45797-6 WOS:000472999700001 |
url |
http://dx.doi.org/10.1038/s41598-019-45797-6 http://hdl.handle.net/11449/184545 |
identifier_str_mv |
Scientific Reports. London: Nature Publishing Group, v. 9, 14 p., 2019. 2045-2322 10.1038/s41598-019-45797-6 WOS:000472999700001 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
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Scientific Reports |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
14 |
dc.publisher.none.fl_str_mv |
Nature Publishing Group |
publisher.none.fl_str_mv |
Nature Publishing Group |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
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UNESP |
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UNESP |
reponame_str |
Repositório Institucional da UNESP |
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Repositório Institucional da UNESP |
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Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
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