The influence of increased water temperature on the duration of spermatogenesis in a neotropical fish, Astyanax altiparanae (Characiformes, Characidae)
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s10695-020-00869-7 http://hdl.handle.net/11449/201007 |
Resumo: | In view of the established climate change scenario and the consequent changes in global temperature, it is essential to study its effects on animal spermatogenesis. Therefore, the aim of this study was to verify the duration of spermatogenesis at different temperatures. For this purpose, 96 male and adult specimens of Astyanax altiparanae were kept in a closed circulation system with water temperature stabilized at 27 °C and 32 °C. Subsequently, the specimens received pulses of BrdU (bromodeoxyuridine) at a concentration of 100 mg/kg/day for 2 consecutive days, and the samples were collected daily for a period of 15 days. Their testes were removed, fixed, processed in historesin, and sectioned in 3 μm, submitted to hematoxylin/eosin staining and to bromodeoxyuridine immunodetection. Partial results of the optimum temperature experiments allowed the classification of A. altiparanae spermatogenic cells in Aund, Adiff, and type B spermatogonia, spermatocytes, spermatids, and spermatozoa. The duration of spermatogenesis was determined as approximately 6 days for animals at a temperature of 27 °C and 1 day for animals at 32 °C. The elevated temperature was also responsible for increasing cell proliferation, resulting in an increase in the number of spermatocytes, spermatids, spermatozoa, and cell death (cell pyknotic). The duration of spermatogenesis in A. altiparanae was directly affected by the elevated water temperature, causing a reduction in the estimated time of spermatogenesis. |
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The influence of increased water temperature on the duration of spermatogenesis in a neotropical fish, Astyanax altiparanae (Characiformes, Characidae)BromodeoxyuridineCharaciformesReproductionYellow-TetraIn view of the established climate change scenario and the consequent changes in global temperature, it is essential to study its effects on animal spermatogenesis. Therefore, the aim of this study was to verify the duration of spermatogenesis at different temperatures. For this purpose, 96 male and adult specimens of Astyanax altiparanae were kept in a closed circulation system with water temperature stabilized at 27 °C and 32 °C. Subsequently, the specimens received pulses of BrdU (bromodeoxyuridine) at a concentration of 100 mg/kg/day for 2 consecutive days, and the samples were collected daily for a period of 15 days. Their testes were removed, fixed, processed in historesin, and sectioned in 3 μm, submitted to hematoxylin/eosin staining and to bromodeoxyuridine immunodetection. Partial results of the optimum temperature experiments allowed the classification of A. altiparanae spermatogenic cells in Aund, Adiff, and type B spermatogonia, spermatocytes, spermatids, and spermatozoa. The duration of spermatogenesis was determined as approximately 6 days for animals at a temperature of 27 °C and 1 day for animals at 32 °C. The elevated temperature was also responsible for increasing cell proliferation, resulting in an increase in the number of spermatocytes, spermatids, spermatozoa, and cell death (cell pyknotic). The duration of spermatogenesis in A. altiparanae was directly affected by the elevated water temperature, causing a reduction in the estimated time of spermatogenesis.Departamento de Biologia e Zootecnia. L.I.NEO - Laboratory of Neotropical Ichthyology UNESP - Universidade Estadual Paulista “Júlio de Mesquita Filho” Câmpus de Ilha Solteira, Rua Monção, n 226, 15385-000Programa de Pós-Graduação em Ciências Biológicas (Zoologia) Instituto de Biociências de Botucatu (UNESP - IBB), R. Prof. Dr. Antonio Celso Wagner Zanin, 250, 18618-689Instituto de Estudo em Saúde e Biologicas (IESB) Research Group of Reproduction on Amazon Fish (GERPA/LaNec) UNIFESSPA - Universidade Federal do Sul e Sudeste do Para, Folha 31, Quadra 07, Lote especial s/n, 68.507-590Departamento de Morfologia Reproductive and Molecular Biology Group UNESP - Universidade Estadual Paulista “Júlio de Mesquita Filho” Câmpus de Botucatu Instituto de Biociências de Botucatu, R. Prof.Dr. Antonio Celso Wagner Zanin, 250, 18618-689Departamento de Biologia e Zootecnia. L.I.NEO - Laboratory of Neotropical Ichthyology UNESP - Universidade Estadual Paulista “Júlio de Mesquita Filho” Câmpus de Ilha Solteira, Rua Monção, n 226, 15385-000Programa de Pós-Graduação em Ciências Biológicas (Zoologia) Instituto de Biociências de Botucatu (UNESP - IBB), R. Prof. Dr. Antonio Celso Wagner Zanin, 250, 18618-689Departamento de Morfologia Reproductive and Molecular Biology Group UNESP - Universidade Estadual Paulista “Júlio de Mesquita Filho” Câmpus de Botucatu Instituto de Biociências de Botucatu, R. Prof.Dr. Antonio Celso Wagner Zanin, 250, 18618-689Universidade Estadual Paulista (Unesp)UNIFESSPA - Universidade Federal do Sul e Sudeste do ParaPostingel Quirino, Patricia [UNESP]da Silva Rodrigues, Maira [UNESP]da Silva Cabral, Elis Marina [UNESP]de Siqueira-Silva, Diógenes HenriqueMori, Ricardo Hideo [UNESP]Butzge, Arno Juliano [UNESP]Nóbrega, Rafael Henrique [UNESP]Ninhaus-Silveira, Alexandre [UNESP]Veríssimo-Silveira, Rosicleire [UNESP]2020-12-12T02:21:44Z2020-12-12T02:21:44Z2020-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1007/s10695-020-00869-7Fish Physiology and Biochemistry.1573-51680920-1742http://hdl.handle.net/11449/20100710.1007/s10695-020-00869-72-s2.0-8509031115229260539849144770000-0002-8298-5004Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengFish Physiology and Biochemistryinfo:eu-repo/semantics/openAccess2021-10-23T15:48:37Zoai:repositorio.unesp.br:11449/201007Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T15:48:37Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
The influence of increased water temperature on the duration of spermatogenesis in a neotropical fish, Astyanax altiparanae (Characiformes, Characidae) |
title |
The influence of increased water temperature on the duration of spermatogenesis in a neotropical fish, Astyanax altiparanae (Characiformes, Characidae) |
spellingShingle |
The influence of increased water temperature on the duration of spermatogenesis in a neotropical fish, Astyanax altiparanae (Characiformes, Characidae) Postingel Quirino, Patricia [UNESP] Bromodeoxyuridine Characiformes Reproduction Yellow-Tetra |
title_short |
The influence of increased water temperature on the duration of spermatogenesis in a neotropical fish, Astyanax altiparanae (Characiformes, Characidae) |
title_full |
The influence of increased water temperature on the duration of spermatogenesis in a neotropical fish, Astyanax altiparanae (Characiformes, Characidae) |
title_fullStr |
The influence of increased water temperature on the duration of spermatogenesis in a neotropical fish, Astyanax altiparanae (Characiformes, Characidae) |
title_full_unstemmed |
The influence of increased water temperature on the duration of spermatogenesis in a neotropical fish, Astyanax altiparanae (Characiformes, Characidae) |
title_sort |
The influence of increased water temperature on the duration of spermatogenesis in a neotropical fish, Astyanax altiparanae (Characiformes, Characidae) |
author |
Postingel Quirino, Patricia [UNESP] |
author_facet |
Postingel Quirino, Patricia [UNESP] da Silva Rodrigues, Maira [UNESP] da Silva Cabral, Elis Marina [UNESP] de Siqueira-Silva, Diógenes Henrique Mori, Ricardo Hideo [UNESP] Butzge, Arno Juliano [UNESP] Nóbrega, Rafael Henrique [UNESP] Ninhaus-Silveira, Alexandre [UNESP] Veríssimo-Silveira, Rosicleire [UNESP] |
author_role |
author |
author2 |
da Silva Rodrigues, Maira [UNESP] da Silva Cabral, Elis Marina [UNESP] de Siqueira-Silva, Diógenes Henrique Mori, Ricardo Hideo [UNESP] Butzge, Arno Juliano [UNESP] Nóbrega, Rafael Henrique [UNESP] Ninhaus-Silveira, Alexandre [UNESP] Veríssimo-Silveira, Rosicleire [UNESP] |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) UNIFESSPA - Universidade Federal do Sul e Sudeste do Para |
dc.contributor.author.fl_str_mv |
Postingel Quirino, Patricia [UNESP] da Silva Rodrigues, Maira [UNESP] da Silva Cabral, Elis Marina [UNESP] de Siqueira-Silva, Diógenes Henrique Mori, Ricardo Hideo [UNESP] Butzge, Arno Juliano [UNESP] Nóbrega, Rafael Henrique [UNESP] Ninhaus-Silveira, Alexandre [UNESP] Veríssimo-Silveira, Rosicleire [UNESP] |
dc.subject.por.fl_str_mv |
Bromodeoxyuridine Characiformes Reproduction Yellow-Tetra |
topic |
Bromodeoxyuridine Characiformes Reproduction Yellow-Tetra |
description |
In view of the established climate change scenario and the consequent changes in global temperature, it is essential to study its effects on animal spermatogenesis. Therefore, the aim of this study was to verify the duration of spermatogenesis at different temperatures. For this purpose, 96 male and adult specimens of Astyanax altiparanae were kept in a closed circulation system with water temperature stabilized at 27 °C and 32 °C. Subsequently, the specimens received pulses of BrdU (bromodeoxyuridine) at a concentration of 100 mg/kg/day for 2 consecutive days, and the samples were collected daily for a period of 15 days. Their testes were removed, fixed, processed in historesin, and sectioned in 3 μm, submitted to hematoxylin/eosin staining and to bromodeoxyuridine immunodetection. Partial results of the optimum temperature experiments allowed the classification of A. altiparanae spermatogenic cells in Aund, Adiff, and type B spermatogonia, spermatocytes, spermatids, and spermatozoa. The duration of spermatogenesis was determined as approximately 6 days for animals at a temperature of 27 °C and 1 day for animals at 32 °C. The elevated temperature was also responsible for increasing cell proliferation, resulting in an increase in the number of spermatocytes, spermatids, spermatozoa, and cell death (cell pyknotic). The duration of spermatogenesis in A. altiparanae was directly affected by the elevated water temperature, causing a reduction in the estimated time of spermatogenesis. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T02:21:44Z 2020-12-12T02:21:44Z 2020-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s10695-020-00869-7 Fish Physiology and Biochemistry. 1573-5168 0920-1742 http://hdl.handle.net/11449/201007 10.1007/s10695-020-00869-7 2-s2.0-85090311152 2926053984914477 0000-0002-8298-5004 |
url |
http://dx.doi.org/10.1007/s10695-020-00869-7 http://hdl.handle.net/11449/201007 |
identifier_str_mv |
Fish Physiology and Biochemistry. 1573-5168 0920-1742 10.1007/s10695-020-00869-7 2-s2.0-85090311152 2926053984914477 0000-0002-8298-5004 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Fish Physiology and Biochemistry |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965711249965056 |