Gene expression of paracoccidioides virulence factors after interaction with macrophages and fibroblasts
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/0074-02760200592 http://hdl.handle.net/11449/207568 |
Resumo: | Paracoccidioidomycosis (PCM) is a systemic mycosis with high prevalence in Latin America that is caused by thermodimorphic fungal species of the Paracoccidioides genus. In this study, we used quantitative PCR (qPCR) to investigate the expression of genes related to the virulence of Paracoccidioides brasiliensis (Pb18) and P. lutzii (Pb01) strains in their mycelial (M) and yeast (Y) forms after contact with alveolar macrophages (AMJ2-C11 cell line) and fibroblasts (MRC-5 cell line). The selected genes were those coding for 43 kDa glycoprotein (gp43), enolase, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), 14-3-3 protein (30 kDa), phospholipase, and aspartyl protease. In the Pb18 M form, the aspartyl protease gene showed the highest expression among all genes tested, both before and after infection of host cells. In the Pb18 Y form after macrophage infection, the 14-3-3 gene showed the highest expression among all genes tested, followed by the phospholipase and gp43 genes, and their expression was 50-fold, 10-fold, and 6-fold higher, respectively, than that in the M form. After fibroblast infection with the Pb18 Y form, the 14-3-3 gene showed the highest expression, followed by the phospholipase and aspartyl protease genes, and their expression was 25-fold, 10-fold, and 10-fold higher, respectively, than that in the M form. Enolase and aspartyl protease genes were expressed upon infection of both cell lines. After macrophage infection with the Pb01 Y form, the 14-3-3 gene showed the highest expression, followed by the phospholipase and aspartyl protease genes, and their expression was 18-fold, 12.5-fold, and 6-fold higher, respectively, than that in the M form. In conclusion, the data show that the expression of the genes analyzed may be upregulated upon fungus-host interaction. Therefore, these genes may be involved in the pathogenesis of paracoccidioidomycosis. |
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Gene expression of paracoccidioides virulence factors after interaction with macrophages and fibroblastsAdhesin genesM formParacoccidioides sppVirulence factorsY formParacoccidioidomycosis (PCM) is a systemic mycosis with high prevalence in Latin America that is caused by thermodimorphic fungal species of the Paracoccidioides genus. In this study, we used quantitative PCR (qPCR) to investigate the expression of genes related to the virulence of Paracoccidioides brasiliensis (Pb18) and P. lutzii (Pb01) strains in their mycelial (M) and yeast (Y) forms after contact with alveolar macrophages (AMJ2-C11 cell line) and fibroblasts (MRC-5 cell line). The selected genes were those coding for 43 kDa glycoprotein (gp43), enolase, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), 14-3-3 protein (30 kDa), phospholipase, and aspartyl protease. In the Pb18 M form, the aspartyl protease gene showed the highest expression among all genes tested, both before and after infection of host cells. In the Pb18 Y form after macrophage infection, the 14-3-3 gene showed the highest expression among all genes tested, followed by the phospholipase and gp43 genes, and their expression was 50-fold, 10-fold, and 6-fold higher, respectively, than that in the M form. After fibroblast infection with the Pb18 Y form, the 14-3-3 gene showed the highest expression, followed by the phospholipase and aspartyl protease genes, and their expression was 25-fold, 10-fold, and 10-fold higher, respectively, than that in the M form. Enolase and aspartyl protease genes were expressed upon infection of both cell lines. After macrophage infection with the Pb01 Y form, the 14-3-3 gene showed the highest expression, followed by the phospholipase and aspartyl protease genes, and their expression was 18-fold, 12.5-fold, and 6-fold higher, respectively, than that in the M form. In conclusion, the data show that the expression of the genes analyzed may be upregulated upon fungus-host interaction. Therefore, these genes may be involved in the pathogenesis of paracoccidioidomycosis.Department of Clinical Analysis Laboratory of Clinical Mycology Faculty of Pharmaceutical Sciences UNESP – Univ Estadual PaulistaSchool of Pharmaceutical Sciences Food and Nutrition Federal University of Mato Grosso do SulDepartment of Cellular and Molecular Biology Ribeirão Preto School of Medicine University of São PauloDepartment of Clinical Analysis Laboratory of Clinical Mycology Faculty of Pharmaceutical Sciences UNESP – Univ Estadual PaulistaUniversidade Estadual Paulista (Unesp)Federal University of Mato Grosso do SulUniversidade de São Paulo (USP)Braz, Jaqueline Derissi [UNESP]Sardi, Janaina de Cássia Orlandi [UNESP]Pitangui, Nayla de Souza [UNESP]Voltan, Aline Raquel [UNESP]Almeida, Ana Marisa Fusco [UNESP]Giannini, Maria José Soares Mendes [UNESP]2021-06-25T10:57:26Z2021-06-25T10:57:26Z2021-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1-24application/pdfhttp://dx.doi.org/10.1590/0074-02760200592Memorias do Instituto Oswaldo Cruz, v. 116, n. 1, p. 1-24, 2021.1678-80600074-0276http://hdl.handle.net/11449/20756810.1590/0074-02760200592S0074-027620210001003062-s2.0-85103745027S0074-02762021000100306.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMemorias do Instituto Oswaldo Cruzinfo:eu-repo/semantics/openAccess2023-10-23T06:04:44Zoai:repositorio.unesp.br:11449/207568Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-10-23T06:04:44Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Gene expression of paracoccidioides virulence factors after interaction with macrophages and fibroblasts |
title |
Gene expression of paracoccidioides virulence factors after interaction with macrophages and fibroblasts |
spellingShingle |
Gene expression of paracoccidioides virulence factors after interaction with macrophages and fibroblasts Braz, Jaqueline Derissi [UNESP] Adhesin genes M form Paracoccidioides spp Virulence factors Y form |
title_short |
Gene expression of paracoccidioides virulence factors after interaction with macrophages and fibroblasts |
title_full |
Gene expression of paracoccidioides virulence factors after interaction with macrophages and fibroblasts |
title_fullStr |
Gene expression of paracoccidioides virulence factors after interaction with macrophages and fibroblasts |
title_full_unstemmed |
Gene expression of paracoccidioides virulence factors after interaction with macrophages and fibroblasts |
title_sort |
Gene expression of paracoccidioides virulence factors after interaction with macrophages and fibroblasts |
author |
Braz, Jaqueline Derissi [UNESP] |
author_facet |
Braz, Jaqueline Derissi [UNESP] Sardi, Janaina de Cássia Orlandi [UNESP] Pitangui, Nayla de Souza [UNESP] Voltan, Aline Raquel [UNESP] Almeida, Ana Marisa Fusco [UNESP] Giannini, Maria José Soares Mendes [UNESP] |
author_role |
author |
author2 |
Sardi, Janaina de Cássia Orlandi [UNESP] Pitangui, Nayla de Souza [UNESP] Voltan, Aline Raquel [UNESP] Almeida, Ana Marisa Fusco [UNESP] Giannini, Maria José Soares Mendes [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Federal University of Mato Grosso do Sul Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Braz, Jaqueline Derissi [UNESP] Sardi, Janaina de Cássia Orlandi [UNESP] Pitangui, Nayla de Souza [UNESP] Voltan, Aline Raquel [UNESP] Almeida, Ana Marisa Fusco [UNESP] Giannini, Maria José Soares Mendes [UNESP] |
dc.subject.por.fl_str_mv |
Adhesin genes M form Paracoccidioides spp Virulence factors Y form |
topic |
Adhesin genes M form Paracoccidioides spp Virulence factors Y form |
description |
Paracoccidioidomycosis (PCM) is a systemic mycosis with high prevalence in Latin America that is caused by thermodimorphic fungal species of the Paracoccidioides genus. In this study, we used quantitative PCR (qPCR) to investigate the expression of genes related to the virulence of Paracoccidioides brasiliensis (Pb18) and P. lutzii (Pb01) strains in their mycelial (M) and yeast (Y) forms after contact with alveolar macrophages (AMJ2-C11 cell line) and fibroblasts (MRC-5 cell line). The selected genes were those coding for 43 kDa glycoprotein (gp43), enolase, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), 14-3-3 protein (30 kDa), phospholipase, and aspartyl protease. In the Pb18 M form, the aspartyl protease gene showed the highest expression among all genes tested, both before and after infection of host cells. In the Pb18 Y form after macrophage infection, the 14-3-3 gene showed the highest expression among all genes tested, followed by the phospholipase and gp43 genes, and their expression was 50-fold, 10-fold, and 6-fold higher, respectively, than that in the M form. After fibroblast infection with the Pb18 Y form, the 14-3-3 gene showed the highest expression, followed by the phospholipase and aspartyl protease genes, and their expression was 25-fold, 10-fold, and 10-fold higher, respectively, than that in the M form. Enolase and aspartyl protease genes were expressed upon infection of both cell lines. After macrophage infection with the Pb01 Y form, the 14-3-3 gene showed the highest expression, followed by the phospholipase and aspartyl protease genes, and their expression was 18-fold, 12.5-fold, and 6-fold higher, respectively, than that in the M form. In conclusion, the data show that the expression of the genes analyzed may be upregulated upon fungus-host interaction. Therefore, these genes may be involved in the pathogenesis of paracoccidioidomycosis. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-25T10:57:26Z 2021-06-25T10:57:26Z 2021-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/0074-02760200592 Memorias do Instituto Oswaldo Cruz, v. 116, n. 1, p. 1-24, 2021. 1678-8060 0074-0276 http://hdl.handle.net/11449/207568 10.1590/0074-02760200592 S0074-02762021000100306 2-s2.0-85103745027 S0074-02762021000100306.pdf |
url |
http://dx.doi.org/10.1590/0074-02760200592 http://hdl.handle.net/11449/207568 |
identifier_str_mv |
Memorias do Instituto Oswaldo Cruz, v. 116, n. 1, p. 1-24, 2021. 1678-8060 0074-0276 10.1590/0074-02760200592 S0074-02762021000100306 2-s2.0-85103745027 S0074-02762021000100306.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Memorias do Instituto Oswaldo Cruz |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1-24 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1799964663985733632 |