Gene Expression in the Salivary Gland of Rhipicephalus (Boophilus) microplus Fed on Tick-Susceptible and Tick-Resistant Hosts

Detalhes bibliográficos
Autor(a) principal: Giachetto, Poliana Fernanda
Data de Publicação: 2020
Outros Autores: Cunha, Rodrigo Casquero, Nhani Jr, Antonio, Garcia, Marcos Valerio, Ferro, Jesus Aparecido [UNESP], Andreotti, Renato
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.3389/fcimb.2019.00477
http://hdl.handle.net/11449/196553
Resumo: The success of cattle tick fixation largely depends on the secretion of substances that alter the immune response of the host. The majority of these substances are expressed by the parasite salivary gland and secreted in tick saliva. It is known that hosts can mount immune responses against ticks and bovine European breeds, and bovine industrial crossbreeds are more susceptible to infestations than are Bos indicus cattle. To identify candidates for the development of novel control strategies for the cattle tick Rhipicephalus (Boophilus) microplus, a salivary gland transcriptome analysis of engorged females fed on susceptible or resistant hosts was performed. Using RNA-Seq, transcriptomes were de novo assembled and produced a total of 235,451 contigs with 93.3% transcriptome completeness. Differential expression analysis identified 137 sequences as differentially expressed genes (DEGs) between ticks raised on tick-susceptible or tick-resistant cattle. DEGs predicted to be secreted proteins include innexins, which are transmembrane proteins that form gap junction channels; the transporters Na+/dicarboxylate, Na+/tricarboxylate, and phosphate transporter and a putative monocarboxylate transporter; a phosphoinositol 4-phosphate adaptor protein; a cysteine-rich protein containing a trypsin inhibitor-like (TIL) domain; a putative defense protein 3 containing a reeler domain; and an F-actin-uncapping protein LRRC16A with a CARMIL_C domain; these genes were upregulated in ticks fed on tick-susceptible cattle. DEGs predicted to be non-secreted proteins included a small heat shock protein and the negative elongation factor B-like, both acting in a coordinated manner to increase HSP transcript levels in the salivary glands of the ticks fed on tick-susceptible cattle; the 26S protease regulatory subunit 6B and another chaperone with similarity to calnexin, also upregulated in ticks fed on tick-susceptible cattle; an EF-hand calcium binding protein and a serine carboxypeptidase (SCP), both involved in the blood coagulation cascade and upregulated in ticks fed on tick-susceptible cattle; and two ribosomal proteins, the 60S acidic ribosomal protein P2 and the 60S ribosomal protein L19. These results help to characterize cattle tick salivary gland gene expression in tick-susceptible and tick-resistant hosts and suggest new putative targets for the control of tick infestations, as those genes involved in the mechanism of stress response during blood feeding.
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spelling Gene Expression in the Salivary Gland of Rhipicephalus (Boophilus) microplus Fed on Tick-Susceptible and Tick-Resistant HostsRNA-Seqcattle ticksialometranscriptomehost-parasite interactionThe success of cattle tick fixation largely depends on the secretion of substances that alter the immune response of the host. The majority of these substances are expressed by the parasite salivary gland and secreted in tick saliva. It is known that hosts can mount immune responses against ticks and bovine European breeds, and bovine industrial crossbreeds are more susceptible to infestations than are Bos indicus cattle. To identify candidates for the development of novel control strategies for the cattle tick Rhipicephalus (Boophilus) microplus, a salivary gland transcriptome analysis of engorged females fed on susceptible or resistant hosts was performed. Using RNA-Seq, transcriptomes were de novo assembled and produced a total of 235,451 contigs with 93.3% transcriptome completeness. Differential expression analysis identified 137 sequences as differentially expressed genes (DEGs) between ticks raised on tick-susceptible or tick-resistant cattle. DEGs predicted to be secreted proteins include innexins, which are transmembrane proteins that form gap junction channels; the transporters Na+/dicarboxylate, Na+/tricarboxylate, and phosphate transporter and a putative monocarboxylate transporter; a phosphoinositol 4-phosphate adaptor protein; a cysteine-rich protein containing a trypsin inhibitor-like (TIL) domain; a putative defense protein 3 containing a reeler domain; and an F-actin-uncapping protein LRRC16A with a CARMIL_C domain; these genes were upregulated in ticks fed on tick-susceptible cattle. DEGs predicted to be non-secreted proteins included a small heat shock protein and the negative elongation factor B-like, both acting in a coordinated manner to increase HSP transcript levels in the salivary glands of the ticks fed on tick-susceptible cattle; the 26S protease regulatory subunit 6B and another chaperone with similarity to calnexin, also upregulated in ticks fed on tick-susceptible cattle; an EF-hand calcium binding protein and a serine carboxypeptidase (SCP), both involved in the blood coagulation cascade and upregulated in ticks fed on tick-susceptible cattle; and two ribosomal proteins, the 60S acidic ribosomal protein P2 and the 60S ribosomal protein L19. These results help to characterize cattle tick salivary gland gene expression in tick-susceptible and tick-resistant hosts and suggest new putative targets for the control of tick infestations, as those genes involved in the mechanism of stress response during blood feeding.Brazilian Agricultural Research Corporation-EmbrapaEmbrapa Agr Informat, Campinas, BrazilUniv Fed Pelotas, Programa Posgrad Biotecnol, CNPq 157460 2018 5, Pelotas, RS, BrazilEmbrapa Beef Cattle, Campo Grande, MS, BrazilSao Paulo State Univ, Dept Technol, Jaboticabal, BrazilSao Paulo State Univ, Dept Technol, Jaboticabal, BrazilBrazilian Agricultural Research Corporation-Embrapa: 01.11.07.002.03.00Frontiers Media SaEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA)Univ Fed PelotasUniversidade Estadual Paulista (Unesp)Giachetto, Poliana FernandaCunha, Rodrigo CasqueroNhani Jr, AntonioGarcia, Marcos ValerioFerro, Jesus Aparecido [UNESP]Andreotti, Renato2020-12-10T19:48:41Z2020-12-10T19:48:41Z2020-01-21info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article15http://dx.doi.org/10.3389/fcimb.2019.00477Frontiers In Cellular And Infection Microbiology. Lausanne: Frontiers Media Sa, v. 9, 15 p., 2020.2235-2988http://hdl.handle.net/11449/19655310.3389/fcimb.2019.00477WOS:000511195300001Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengFrontiers In Cellular And Infection Microbiologyinfo:eu-repo/semantics/openAccess2024-06-07T15:32:49Zoai:repositorio.unesp.br:11449/196553Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-06T00:04:53.782383Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Gene Expression in the Salivary Gland of Rhipicephalus (Boophilus) microplus Fed on Tick-Susceptible and Tick-Resistant Hosts
title Gene Expression in the Salivary Gland of Rhipicephalus (Boophilus) microplus Fed on Tick-Susceptible and Tick-Resistant Hosts
spellingShingle Gene Expression in the Salivary Gland of Rhipicephalus (Boophilus) microplus Fed on Tick-Susceptible and Tick-Resistant Hosts
Giachetto, Poliana Fernanda
RNA-Seq
cattle tick
sialome
transcriptome
host-parasite interaction
title_short Gene Expression in the Salivary Gland of Rhipicephalus (Boophilus) microplus Fed on Tick-Susceptible and Tick-Resistant Hosts
title_full Gene Expression in the Salivary Gland of Rhipicephalus (Boophilus) microplus Fed on Tick-Susceptible and Tick-Resistant Hosts
title_fullStr Gene Expression in the Salivary Gland of Rhipicephalus (Boophilus) microplus Fed on Tick-Susceptible and Tick-Resistant Hosts
title_full_unstemmed Gene Expression in the Salivary Gland of Rhipicephalus (Boophilus) microplus Fed on Tick-Susceptible and Tick-Resistant Hosts
title_sort Gene Expression in the Salivary Gland of Rhipicephalus (Boophilus) microplus Fed on Tick-Susceptible and Tick-Resistant Hosts
author Giachetto, Poliana Fernanda
author_facet Giachetto, Poliana Fernanda
Cunha, Rodrigo Casquero
Nhani Jr, Antonio
Garcia, Marcos Valerio
Ferro, Jesus Aparecido [UNESP]
Andreotti, Renato
author_role author
author2 Cunha, Rodrigo Casquero
Nhani Jr, Antonio
Garcia, Marcos Valerio
Ferro, Jesus Aparecido [UNESP]
Andreotti, Renato
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
Univ Fed Pelotas
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Giachetto, Poliana Fernanda
Cunha, Rodrigo Casquero
Nhani Jr, Antonio
Garcia, Marcos Valerio
Ferro, Jesus Aparecido [UNESP]
Andreotti, Renato
dc.subject.por.fl_str_mv RNA-Seq
cattle tick
sialome
transcriptome
host-parasite interaction
topic RNA-Seq
cattle tick
sialome
transcriptome
host-parasite interaction
description The success of cattle tick fixation largely depends on the secretion of substances that alter the immune response of the host. The majority of these substances are expressed by the parasite salivary gland and secreted in tick saliva. It is known that hosts can mount immune responses against ticks and bovine European breeds, and bovine industrial crossbreeds are more susceptible to infestations than are Bos indicus cattle. To identify candidates for the development of novel control strategies for the cattle tick Rhipicephalus (Boophilus) microplus, a salivary gland transcriptome analysis of engorged females fed on susceptible or resistant hosts was performed. Using RNA-Seq, transcriptomes were de novo assembled and produced a total of 235,451 contigs with 93.3% transcriptome completeness. Differential expression analysis identified 137 sequences as differentially expressed genes (DEGs) between ticks raised on tick-susceptible or tick-resistant cattle. DEGs predicted to be secreted proteins include innexins, which are transmembrane proteins that form gap junction channels; the transporters Na+/dicarboxylate, Na+/tricarboxylate, and phosphate transporter and a putative monocarboxylate transporter; a phosphoinositol 4-phosphate adaptor protein; a cysteine-rich protein containing a trypsin inhibitor-like (TIL) domain; a putative defense protein 3 containing a reeler domain; and an F-actin-uncapping protein LRRC16A with a CARMIL_C domain; these genes were upregulated in ticks fed on tick-susceptible cattle. DEGs predicted to be non-secreted proteins included a small heat shock protein and the negative elongation factor B-like, both acting in a coordinated manner to increase HSP transcript levels in the salivary glands of the ticks fed on tick-susceptible cattle; the 26S protease regulatory subunit 6B and another chaperone with similarity to calnexin, also upregulated in ticks fed on tick-susceptible cattle; an EF-hand calcium binding protein and a serine carboxypeptidase (SCP), both involved in the blood coagulation cascade and upregulated in ticks fed on tick-susceptible cattle; and two ribosomal proteins, the 60S acidic ribosomal protein P2 and the 60S ribosomal protein L19. These results help to characterize cattle tick salivary gland gene expression in tick-susceptible and tick-resistant hosts and suggest new putative targets for the control of tick infestations, as those genes involved in the mechanism of stress response during blood feeding.
publishDate 2020
dc.date.none.fl_str_mv 2020-12-10T19:48:41Z
2020-12-10T19:48:41Z
2020-01-21
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.3389/fcimb.2019.00477
Frontiers In Cellular And Infection Microbiology. Lausanne: Frontiers Media Sa, v. 9, 15 p., 2020.
2235-2988
http://hdl.handle.net/11449/196553
10.3389/fcimb.2019.00477
WOS:000511195300001
url http://dx.doi.org/10.3389/fcimb.2019.00477
http://hdl.handle.net/11449/196553
identifier_str_mv Frontiers In Cellular And Infection Microbiology. Lausanne: Frontiers Media Sa, v. 9, 15 p., 2020.
2235-2988
10.3389/fcimb.2019.00477
WOS:000511195300001
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Frontiers In Cellular And Infection Microbiology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 15
dc.publisher.none.fl_str_mv Frontiers Media Sa
publisher.none.fl_str_mv Frontiers Media Sa
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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