A Novel Synthesis of a Magnetic Porous Imprinted Polymer by Polyol Method Coupled with Electrochemical Biomimetic Sensor for the Detection of Folate in Food Samples

Detalhes bibliográficos
Autor(a) principal: Khan, Sabir [UNESP]
Data de Publicação: 2022
Outros Autores: Wong, Ademar [UNESP], Rychlik, Michael, Sotomayor, María del Pilar Taboada [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.3390/chemosensors10110473
http://hdl.handle.net/11449/249738
Resumo: The present study reports the development and application of a novel, sensitive, and selective voltammetric sensor for the quantitation of folate or vitamin B9 in foodstuffs. The sensor was made from magnetic molecularly imprinted polymers (MMIPs), which were synthesized by the core–shell method using magnetite nanoparticles obtained by the polyol method. The MMIP-based sensor was used for the selective and specific detection of folate in different food samples. The MMIP material was constructed using magnetic water-dispersible nanomaterial, which was prepared by immersing iron (III) acetylacetonate in tri-ethylene-glycol (TEG) solvent. The magnetic water-dispersible nanomaterial was then subjected to polymerization using allyl alcohol as a functional monomer, ethylene-glycol-dimethacrylate (EGDMA) as a cross-linking agent, and 2,2-Azobisisobutyronitrile (AIBN) as a radical initiator. The proposed magnetic materials were characterized by Brunauer–Emmett–Teller (BET), field emission gun scanning electron microscopy (FEG-SEM), thermogravimetric analysis (TGA), and vibrating sample magnetometer (VSM) analysis. The quantification of folate was performed by square wave voltammetry under optimized conditions using 15 mg of MMIPs and 85 mg of carbon paste. The modified electrode presented a linear dynamic range (LDR) of 2.0–12 µmol L−1 and a limit of detection (LOD) of 1.0 × 10−7 mol L−1 in 0.1 mol L−1 acetate buffer solution (pH 4.0). The proposed sensor was successfully applied for folate detection in different food samples, where recovery percentages ranging from 93 to 103% were obtained. Finally, the results obtained from the analysis of selectivity showed that the modified biomimetic sensor is highly efficient for folate determination in real food samples. Adsorption tests were used to evaluate and compare the efficiency of the MMIPs and magnetic non-molecularly imprinted polymer (MNIPs)—used as control material, through the application of HPLC as a standard method.
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spelling A Novel Synthesis of a Magnetic Porous Imprinted Polymer by Polyol Method Coupled with Electrochemical Biomimetic Sensor for the Detection of Folate in Food Sampleselectrochemical sensorfolateMMIPsreal samplesThe present study reports the development and application of a novel, sensitive, and selective voltammetric sensor for the quantitation of folate or vitamin B9 in foodstuffs. The sensor was made from magnetic molecularly imprinted polymers (MMIPs), which were synthesized by the core–shell method using magnetite nanoparticles obtained by the polyol method. The MMIP-based sensor was used for the selective and specific detection of folate in different food samples. The MMIP material was constructed using magnetic water-dispersible nanomaterial, which was prepared by immersing iron (III) acetylacetonate in tri-ethylene-glycol (TEG) solvent. The magnetic water-dispersible nanomaterial was then subjected to polymerization using allyl alcohol as a functional monomer, ethylene-glycol-dimethacrylate (EGDMA) as a cross-linking agent, and 2,2-Azobisisobutyronitrile (AIBN) as a radical initiator. The proposed magnetic materials were characterized by Brunauer–Emmett–Teller (BET), field emission gun scanning electron microscopy (FEG-SEM), thermogravimetric analysis (TGA), and vibrating sample magnetometer (VSM) analysis. The quantification of folate was performed by square wave voltammetry under optimized conditions using 15 mg of MMIPs and 85 mg of carbon paste. The modified electrode presented a linear dynamic range (LDR) of 2.0–12 µmol L−1 and a limit of detection (LOD) of 1.0 × 10−7 mol L−1 in 0.1 mol L−1 acetate buffer solution (pH 4.0). The proposed sensor was successfully applied for folate detection in different food samples, where recovery percentages ranging from 93 to 103% were obtained. Finally, the results obtained from the analysis of selectivity showed that the modified biomimetic sensor is highly efficient for folate determination in real food samples. Adsorption tests were used to evaluate and compare the efficiency of the MMIPs and magnetic non-molecularly imprinted polymer (MNIPs)—used as control material, through the application of HPLC as a standard method.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Natural Sciences Mathematics and Statistics Federal Rural University of the Semi-AridInstitute of Chemistry São Paulo State University (UNESP)Analytical Food Chemistry School of Life Sciences Technical University of Munich, Maximus-von-Imhof-Forum 2National Institute of Alternative Technologies for Detection Toxicological Evaluation and Removal of Micropollutants and Radioactives (INCT-DATREM)Institute of Chemistry São Paulo State University (UNESP)CNPq: #151717/2018-4FAPESP: #2014/50945-4FAPESP: #2019/00677-7CNPq: #301728/2019-4CNPq: #408050/2018 7CNPq: #465571/2014-0Federal Rural University of the Semi-AridUniversidade Estadual Paulista (UNESP)Technical University of MunichToxicological Evaluation and Removal of Micropollutants and Radioactives (INCT-DATREM)Khan, Sabir [UNESP]Wong, Ademar [UNESP]Rychlik, MichaelSotomayor, María del Pilar Taboada [UNESP]2023-07-29T16:07:50Z2023-07-29T16:07:50Z2022-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3390/chemosensors10110473Chemosensors, v. 10, n. 11, 2022.2227-9040http://hdl.handle.net/11449/24973810.3390/chemosensors101104732-s2.0-85149484287Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengChemosensorsinfo:eu-repo/semantics/openAccess2023-07-29T16:07:50Zoai:repositorio.unesp.br:11449/249738Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T14:05:02.171239Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv A Novel Synthesis of a Magnetic Porous Imprinted Polymer by Polyol Method Coupled with Electrochemical Biomimetic Sensor for the Detection of Folate in Food Samples
title A Novel Synthesis of a Magnetic Porous Imprinted Polymer by Polyol Method Coupled with Electrochemical Biomimetic Sensor for the Detection of Folate in Food Samples
spellingShingle A Novel Synthesis of a Magnetic Porous Imprinted Polymer by Polyol Method Coupled with Electrochemical Biomimetic Sensor for the Detection of Folate in Food Samples
Khan, Sabir [UNESP]
electrochemical sensor
folate
MMIPs
real samples
title_short A Novel Synthesis of a Magnetic Porous Imprinted Polymer by Polyol Method Coupled with Electrochemical Biomimetic Sensor for the Detection of Folate in Food Samples
title_full A Novel Synthesis of a Magnetic Porous Imprinted Polymer by Polyol Method Coupled with Electrochemical Biomimetic Sensor for the Detection of Folate in Food Samples
title_fullStr A Novel Synthesis of a Magnetic Porous Imprinted Polymer by Polyol Method Coupled with Electrochemical Biomimetic Sensor for the Detection of Folate in Food Samples
title_full_unstemmed A Novel Synthesis of a Magnetic Porous Imprinted Polymer by Polyol Method Coupled with Electrochemical Biomimetic Sensor for the Detection of Folate in Food Samples
title_sort A Novel Synthesis of a Magnetic Porous Imprinted Polymer by Polyol Method Coupled with Electrochemical Biomimetic Sensor for the Detection of Folate in Food Samples
author Khan, Sabir [UNESP]
author_facet Khan, Sabir [UNESP]
Wong, Ademar [UNESP]
Rychlik, Michael
Sotomayor, María del Pilar Taboada [UNESP]
author_role author
author2 Wong, Ademar [UNESP]
Rychlik, Michael
Sotomayor, María del Pilar Taboada [UNESP]
author2_role author
author
author
dc.contributor.none.fl_str_mv Federal Rural University of the Semi-Arid
Universidade Estadual Paulista (UNESP)
Technical University of Munich
Toxicological Evaluation and Removal of Micropollutants and Radioactives (INCT-DATREM)
dc.contributor.author.fl_str_mv Khan, Sabir [UNESP]
Wong, Ademar [UNESP]
Rychlik, Michael
Sotomayor, María del Pilar Taboada [UNESP]
dc.subject.por.fl_str_mv electrochemical sensor
folate
MMIPs
real samples
topic electrochemical sensor
folate
MMIPs
real samples
description The present study reports the development and application of a novel, sensitive, and selective voltammetric sensor for the quantitation of folate or vitamin B9 in foodstuffs. The sensor was made from magnetic molecularly imprinted polymers (MMIPs), which were synthesized by the core–shell method using magnetite nanoparticles obtained by the polyol method. The MMIP-based sensor was used for the selective and specific detection of folate in different food samples. The MMIP material was constructed using magnetic water-dispersible nanomaterial, which was prepared by immersing iron (III) acetylacetonate in tri-ethylene-glycol (TEG) solvent. The magnetic water-dispersible nanomaterial was then subjected to polymerization using allyl alcohol as a functional monomer, ethylene-glycol-dimethacrylate (EGDMA) as a cross-linking agent, and 2,2-Azobisisobutyronitrile (AIBN) as a radical initiator. The proposed magnetic materials were characterized by Brunauer–Emmett–Teller (BET), field emission gun scanning electron microscopy (FEG-SEM), thermogravimetric analysis (TGA), and vibrating sample magnetometer (VSM) analysis. The quantification of folate was performed by square wave voltammetry under optimized conditions using 15 mg of MMIPs and 85 mg of carbon paste. The modified electrode presented a linear dynamic range (LDR) of 2.0–12 µmol L−1 and a limit of detection (LOD) of 1.0 × 10−7 mol L−1 in 0.1 mol L−1 acetate buffer solution (pH 4.0). The proposed sensor was successfully applied for folate detection in different food samples, where recovery percentages ranging from 93 to 103% were obtained. Finally, the results obtained from the analysis of selectivity showed that the modified biomimetic sensor is highly efficient for folate determination in real food samples. Adsorption tests were used to evaluate and compare the efficiency of the MMIPs and magnetic non-molecularly imprinted polymer (MNIPs)—used as control material, through the application of HPLC as a standard method.
publishDate 2022
dc.date.none.fl_str_mv 2022-11-01
2023-07-29T16:07:50Z
2023-07-29T16:07:50Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.3390/chemosensors10110473
Chemosensors, v. 10, n. 11, 2022.
2227-9040
http://hdl.handle.net/11449/249738
10.3390/chemosensors10110473
2-s2.0-85149484287
url http://dx.doi.org/10.3390/chemosensors10110473
http://hdl.handle.net/11449/249738
identifier_str_mv Chemosensors, v. 10, n. 11, 2022.
2227-9040
10.3390/chemosensors10110473
2-s2.0-85149484287
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Chemosensors
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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