Biodentine and MTA modulate immunoinflammatory response favoring bone formation in sealing of furcation perforations in rat molars
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s00784-018-2550-7 http://hdl.handle.net/11449/180015 |
Resumo: | Objectives: Evaluate the tissue reaction of periodontium subjacent to furcation perforations in rat molars sealed with Biodentine or mineral trioxide aggregate (MTA). Materials and methods: The pulp chamber floor of right upper first molars of 60 rats was perforated and filled with Biodentine, MTA, or cotton pellet (sham); the left first molars were used as control. After 7, 15, 30, and 60 days, maxillary fragments were processed for paraffin-embedding. The periodontal space (PS), volume density of inflammatory cells (VvIC) and fibroblasts (VvFb), number of osteoclasts, and collagen content were obtained. Interleukin-6 (IL-6) and osterix (osteoblast marker) were detected by immunohistochemistry. The data were submitted to ANOVA and Tukey’s test (p ≤ 0.05). Results: At 7 days, high values in VvIC, IL-6-immunolabeled cells, and osteoclasts were accompanied by reduced collagen content in enlarged PS of experimental groups. At all periods, VvIC, number of osteoclasts and IL-6, and PS were higher in sham than in Biodentine and MTA (p < 0.0001). From 7 to 60 days, significant reduction in VvIC, IL-6 immunoexpression, and osteoclasts was accompanied by significant increase in VvFb, osteoblasts, and collagen in Biodentine and MTA groups. At 60 days, significant differences in VvIC, PS, IL-6, osteoclasts, and osteoblasts were not found between Biodentine and MTA. Significant differences in the osteoclast number were not observed among Biodentine, MTA, and control groups while osteoblasts number was higher in Biodentine and MTA groups. Conclusions: Despite the initial inflammatory reaction and bone resorption, the sealing of furcation perforations with Biodentine and MTA favors the repair of periodontal tissues. Clinical relevance: Biodentine and MTA exhibit potential as repair material in the treatment of furcation perforations. |
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Biodentine and MTA modulate immunoinflammatory response favoring bone formation in sealing of furcation perforations in rat molarsBone remodelingCalcium silicate cementImmunohistochemistryPeriodontium repairObjectives: Evaluate the tissue reaction of periodontium subjacent to furcation perforations in rat molars sealed with Biodentine or mineral trioxide aggregate (MTA). Materials and methods: The pulp chamber floor of right upper first molars of 60 rats was perforated and filled with Biodentine, MTA, or cotton pellet (sham); the left first molars were used as control. After 7, 15, 30, and 60 days, maxillary fragments were processed for paraffin-embedding. The periodontal space (PS), volume density of inflammatory cells (VvIC) and fibroblasts (VvFb), number of osteoclasts, and collagen content were obtained. Interleukin-6 (IL-6) and osterix (osteoblast marker) were detected by immunohistochemistry. The data were submitted to ANOVA and Tukey’s test (p ≤ 0.05). Results: At 7 days, high values in VvIC, IL-6-immunolabeled cells, and osteoclasts were accompanied by reduced collagen content in enlarged PS of experimental groups. At all periods, VvIC, number of osteoclasts and IL-6, and PS were higher in sham than in Biodentine and MTA (p < 0.0001). From 7 to 60 days, significant reduction in VvIC, IL-6 immunoexpression, and osteoclasts was accompanied by significant increase in VvFb, osteoblasts, and collagen in Biodentine and MTA groups. At 60 days, significant differences in VvIC, PS, IL-6, osteoclasts, and osteoblasts were not found between Biodentine and MTA. Significant differences in the osteoclast number were not observed among Biodentine, MTA, and control groups while osteoblasts number was higher in Biodentine and MTA groups. Conclusions: Despite the initial inflammatory reaction and bone resorption, the sealing of furcation perforations with Biodentine and MTA favors the repair of periodontal tissues. Clinical relevance: Biodentine and MTA exhibit potential as repair material in the treatment of furcation perforations.UNESP- Univ. Estadual Paulista Dental School Department of Restorative DentistrySchool of Dentistry (USC-Bauru) Pro-Rectory of Research and Post Graduation Universidade Sagrado CoraçãoUNESP - Univ Estadual Paulista Dental School Department of Morphology Laboratory of Histology and Embryology, Rua Humaitá, 1680, CentroUNESP- Univ. Estadual Paulista Dental School Department of Restorative DentistryUNESP - Univ Estadual Paulista Dental School Department of Morphology Laboratory of Histology and Embryology, Rua Humaitá, 1680, CentroUniversidade Estadual Paulista (Unesp)Universidade Sagrado Coraçãoda Fonseca, Tiago Silva [UNESP]Silva, Guilherme F.Guerreiro-Tanomaru, Juliane M. [UNESP]Delfino, Mateus Machado [UNESP]Sasso-Cerri, Estela [UNESP]Tanomaru-Filho, Mário [UNESP]Cerri, Paulo Sérgio [UNESP]2018-12-11T17:37:41Z2018-12-11T17:37:41Z2018-07-07info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1-16http://dx.doi.org/10.1007/s00784-018-2550-7Clinical Oral Investigations, p. 1-16.1436-37711432-6981http://hdl.handle.net/11449/18001510.1007/s00784-018-2550-72-s2.0-850496024272-s2.0-85049602427.pdf32784959112078820000-0001-5756-5828Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengClinical Oral Investigations0,9860,986info:eu-repo/semantics/openAccess2024-09-27T15:15:10Zoai:repositorio.unesp.br:11449/180015Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-27T15:15:10Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Biodentine and MTA modulate immunoinflammatory response favoring bone formation in sealing of furcation perforations in rat molars |
title |
Biodentine and MTA modulate immunoinflammatory response favoring bone formation in sealing of furcation perforations in rat molars |
spellingShingle |
Biodentine and MTA modulate immunoinflammatory response favoring bone formation in sealing of furcation perforations in rat molars da Fonseca, Tiago Silva [UNESP] Bone remodeling Calcium silicate cement Immunohistochemistry Periodontium repair |
title_short |
Biodentine and MTA modulate immunoinflammatory response favoring bone formation in sealing of furcation perforations in rat molars |
title_full |
Biodentine and MTA modulate immunoinflammatory response favoring bone formation in sealing of furcation perforations in rat molars |
title_fullStr |
Biodentine and MTA modulate immunoinflammatory response favoring bone formation in sealing of furcation perforations in rat molars |
title_full_unstemmed |
Biodentine and MTA modulate immunoinflammatory response favoring bone formation in sealing of furcation perforations in rat molars |
title_sort |
Biodentine and MTA modulate immunoinflammatory response favoring bone formation in sealing of furcation perforations in rat molars |
author |
da Fonseca, Tiago Silva [UNESP] |
author_facet |
da Fonseca, Tiago Silva [UNESP] Silva, Guilherme F. Guerreiro-Tanomaru, Juliane M. [UNESP] Delfino, Mateus Machado [UNESP] Sasso-Cerri, Estela [UNESP] Tanomaru-Filho, Mário [UNESP] Cerri, Paulo Sérgio [UNESP] |
author_role |
author |
author2 |
Silva, Guilherme F. Guerreiro-Tanomaru, Juliane M. [UNESP] Delfino, Mateus Machado [UNESP] Sasso-Cerri, Estela [UNESP] Tanomaru-Filho, Mário [UNESP] Cerri, Paulo Sérgio [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade Sagrado Coração |
dc.contributor.author.fl_str_mv |
da Fonseca, Tiago Silva [UNESP] Silva, Guilherme F. Guerreiro-Tanomaru, Juliane M. [UNESP] Delfino, Mateus Machado [UNESP] Sasso-Cerri, Estela [UNESP] Tanomaru-Filho, Mário [UNESP] Cerri, Paulo Sérgio [UNESP] |
dc.subject.por.fl_str_mv |
Bone remodeling Calcium silicate cement Immunohistochemistry Periodontium repair |
topic |
Bone remodeling Calcium silicate cement Immunohistochemistry Periodontium repair |
description |
Objectives: Evaluate the tissue reaction of periodontium subjacent to furcation perforations in rat molars sealed with Biodentine or mineral trioxide aggregate (MTA). Materials and methods: The pulp chamber floor of right upper first molars of 60 rats was perforated and filled with Biodentine, MTA, or cotton pellet (sham); the left first molars were used as control. After 7, 15, 30, and 60 days, maxillary fragments were processed for paraffin-embedding. The periodontal space (PS), volume density of inflammatory cells (VvIC) and fibroblasts (VvFb), number of osteoclasts, and collagen content were obtained. Interleukin-6 (IL-6) and osterix (osteoblast marker) were detected by immunohistochemistry. The data were submitted to ANOVA and Tukey’s test (p ≤ 0.05). Results: At 7 days, high values in VvIC, IL-6-immunolabeled cells, and osteoclasts were accompanied by reduced collagen content in enlarged PS of experimental groups. At all periods, VvIC, number of osteoclasts and IL-6, and PS were higher in sham than in Biodentine and MTA (p < 0.0001). From 7 to 60 days, significant reduction in VvIC, IL-6 immunoexpression, and osteoclasts was accompanied by significant increase in VvFb, osteoblasts, and collagen in Biodentine and MTA groups. At 60 days, significant differences in VvIC, PS, IL-6, osteoclasts, and osteoblasts were not found between Biodentine and MTA. Significant differences in the osteoclast number were not observed among Biodentine, MTA, and control groups while osteoblasts number was higher in Biodentine and MTA groups. Conclusions: Despite the initial inflammatory reaction and bone resorption, the sealing of furcation perforations with Biodentine and MTA favors the repair of periodontal tissues. Clinical relevance: Biodentine and MTA exhibit potential as repair material in the treatment of furcation perforations. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-11T17:37:41Z 2018-12-11T17:37:41Z 2018-07-07 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s00784-018-2550-7 Clinical Oral Investigations, p. 1-16. 1436-3771 1432-6981 http://hdl.handle.net/11449/180015 10.1007/s00784-018-2550-7 2-s2.0-85049602427 2-s2.0-85049602427.pdf 3278495911207882 0000-0001-5756-5828 |
url |
http://dx.doi.org/10.1007/s00784-018-2550-7 http://hdl.handle.net/11449/180015 |
identifier_str_mv |
Clinical Oral Investigations, p. 1-16. 1436-3771 1432-6981 10.1007/s00784-018-2550-7 2-s2.0-85049602427 2-s2.0-85049602427.pdf 3278495911207882 0000-0001-5756-5828 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Clinical Oral Investigations 0,986 0,986 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1-16 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
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1813546450635718656 |