Anti-inflammatory activity of Vismia guianensis (Aubl.) Pers. extracts and antifungal activity against Sporothrix schenckii
Autor(a) principal: | |
---|---|
Data de Publicação: | 2017 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.jep.2016.11.030 http://hdl.handle.net/11449/169276 |
Resumo: | Ethnopharmacological relevance Vismia guianensis (Aubl.) Pers. is traditionally used in North and Northeast of Brazil for the treatment of dermatomycoses. Since the strategy associating immunomodulators with antifungal drugs seems to be promissory to improve the treatment efficacy in fungal infections, we aimed to investigate the antifungal activity of V. guianensis ethanolic extract of leaves (VGL) and bark (VGB) against Sporothrix schenckii ATCC 16345 and their antinflammatory activities. Material and methods The extracts were analyzed by HPLC-DAD-IT MS/MS for in situ identification of major compounds. Antifungal activity was evaluated in vitro (microdilution test) and in vivo using a murine model of S. schenckii infection. The production of TNF-α, IFN-γ, IL-4, IL-10 and IL-12 by measured by ELISA, as well as measured the production and inhibition of the NO after treatment with the plant extracts or itraconazole (ITR). Results Two O-glucosyl-flavonoids and 16 prenylated benzophenone derivatives already described for Vismia were detected. Both VGL and VGB showed significant antifungal activity either in in vitro assay of microdilution (MIC=3.9 µg/mL) and in vivo model of infection with reduction of S. schenckii load in spleen. It was also observed a predominance of reduction in the production of NO and the proinflammatory cytokines evaluated except TNFα, but with stimulation of IL-10, as evidence of a potential anti-inflammatory effect associated. Conclusion The results showed that both VGL and VGB have a significant antifungal against S. schenckii and an anti-inflammatory activity. These results can support the use of these extracts for alternative treatment of sporotrichosis. |
id |
UNSP_156f3350d7dc93c23fc33b57b0c9900b |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/169276 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Anti-inflammatory activity of Vismia guianensis (Aubl.) Pers. extracts and antifungal activity against Sporothrix schenckiiAnti-inflammatoryAntifungalPrenylated benzophenone, Sporothrix schenckiiSporotrichosisVismia guianensisEthnopharmacological relevance Vismia guianensis (Aubl.) Pers. is traditionally used in North and Northeast of Brazil for the treatment of dermatomycoses. Since the strategy associating immunomodulators with antifungal drugs seems to be promissory to improve the treatment efficacy in fungal infections, we aimed to investigate the antifungal activity of V. guianensis ethanolic extract of leaves (VGL) and bark (VGB) against Sporothrix schenckii ATCC 16345 and their antinflammatory activities. Material and methods The extracts were analyzed by HPLC-DAD-IT MS/MS for in situ identification of major compounds. Antifungal activity was evaluated in vitro (microdilution test) and in vivo using a murine model of S. schenckii infection. The production of TNF-α, IFN-γ, IL-4, IL-10 and IL-12 by measured by ELISA, as well as measured the production and inhibition of the NO after treatment with the plant extracts or itraconazole (ITR). Results Two O-glucosyl-flavonoids and 16 prenylated benzophenone derivatives already described for Vismia were detected. Both VGL and VGB showed significant antifungal activity either in in vitro assay of microdilution (MIC=3.9 µg/mL) and in vivo model of infection with reduction of S. schenckii load in spleen. It was also observed a predominance of reduction in the production of NO and the proinflammatory cytokines evaluated except TNFα, but with stimulation of IL-10, as evidence of a potential anti-inflammatory effect associated. Conclusion The results showed that both VGL and VGB have a significant antifungal against S. schenckii and an anti-inflammatory activity. These results can support the use of these extracts for alternative treatment of sporotrichosis.Faculdade de Ciências Farmacêuticas. Universidade Estadual Paulista Julio Mesquita Filho UNESP, Rod. Araraquara-Jaú - Km 1 –s/n -CEP: 14800-903Faculdade de Ciências Farmacêuticas de Riberão Preto Universidade Estadual de São Paulo Departamento de Física e QuímicaFaculdade de Ciências Farmacêuticas. Universidade Estadual Paulista Julio Mesquita Filho UNESP, Rod. Araraquara-Jaú - Km 1 –s/n -CEP: 14800-903Faculdade de Ciências Farmacêuticas de Riberão Preto Universidade Estadual de São Paulo Departamento de Física e QuímicaUniversidade Estadual Paulista (Unesp)Oliveira, A. H. [UNESP]de Oliveira, G. G. [UNESP]Carnevale Neto, F. [UNESP]Portuondo, D. F. [UNESP]Batista-Duharte, A. [UNESP]Carlos, I. Z. [UNESP]2018-12-11T16:45:10Z2018-12-11T16:45:10Z2017-01-04info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1application/pdfhttp://dx.doi.org/10.1016/j.jep.2016.11.030Journal of Ethnopharmacology, v. 195, p. 1-.1872-75730378-8741http://hdl.handle.net/11449/16927610.1016/j.jep.2016.11.0302-s2.0-850071741692-s2.0-85007174169.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Ethnopharmacology1,150info:eu-repo/semantics/openAccess2023-12-14T06:16:57Zoai:repositorio.unesp.br:11449/169276Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:16:13.240731Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Anti-inflammatory activity of Vismia guianensis (Aubl.) Pers. extracts and antifungal activity against Sporothrix schenckii |
title |
Anti-inflammatory activity of Vismia guianensis (Aubl.) Pers. extracts and antifungal activity against Sporothrix schenckii |
spellingShingle |
Anti-inflammatory activity of Vismia guianensis (Aubl.) Pers. extracts and antifungal activity against Sporothrix schenckii Oliveira, A. H. [UNESP] Anti-inflammatory Antifungal Prenylated benzophenone, Sporothrix schenckii Sporotrichosis Vismia guianensis |
title_short |
Anti-inflammatory activity of Vismia guianensis (Aubl.) Pers. extracts and antifungal activity against Sporothrix schenckii |
title_full |
Anti-inflammatory activity of Vismia guianensis (Aubl.) Pers. extracts and antifungal activity against Sporothrix schenckii |
title_fullStr |
Anti-inflammatory activity of Vismia guianensis (Aubl.) Pers. extracts and antifungal activity against Sporothrix schenckii |
title_full_unstemmed |
Anti-inflammatory activity of Vismia guianensis (Aubl.) Pers. extracts and antifungal activity against Sporothrix schenckii |
title_sort |
Anti-inflammatory activity of Vismia guianensis (Aubl.) Pers. extracts and antifungal activity against Sporothrix schenckii |
author |
Oliveira, A. H. [UNESP] |
author_facet |
Oliveira, A. H. [UNESP] de Oliveira, G. G. [UNESP] Carnevale Neto, F. [UNESP] Portuondo, D. F. [UNESP] Batista-Duharte, A. [UNESP] Carlos, I. Z. [UNESP] |
author_role |
author |
author2 |
de Oliveira, G. G. [UNESP] Carnevale Neto, F. [UNESP] Portuondo, D. F. [UNESP] Batista-Duharte, A. [UNESP] Carlos, I. Z. [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Oliveira, A. H. [UNESP] de Oliveira, G. G. [UNESP] Carnevale Neto, F. [UNESP] Portuondo, D. F. [UNESP] Batista-Duharte, A. [UNESP] Carlos, I. Z. [UNESP] |
dc.subject.por.fl_str_mv |
Anti-inflammatory Antifungal Prenylated benzophenone, Sporothrix schenckii Sporotrichosis Vismia guianensis |
topic |
Anti-inflammatory Antifungal Prenylated benzophenone, Sporothrix schenckii Sporotrichosis Vismia guianensis |
description |
Ethnopharmacological relevance Vismia guianensis (Aubl.) Pers. is traditionally used in North and Northeast of Brazil for the treatment of dermatomycoses. Since the strategy associating immunomodulators with antifungal drugs seems to be promissory to improve the treatment efficacy in fungal infections, we aimed to investigate the antifungal activity of V. guianensis ethanolic extract of leaves (VGL) and bark (VGB) against Sporothrix schenckii ATCC 16345 and their antinflammatory activities. Material and methods The extracts were analyzed by HPLC-DAD-IT MS/MS for in situ identification of major compounds. Antifungal activity was evaluated in vitro (microdilution test) and in vivo using a murine model of S. schenckii infection. The production of TNF-α, IFN-γ, IL-4, IL-10 and IL-12 by measured by ELISA, as well as measured the production and inhibition of the NO after treatment with the plant extracts or itraconazole (ITR). Results Two O-glucosyl-flavonoids and 16 prenylated benzophenone derivatives already described for Vismia were detected. Both VGL and VGB showed significant antifungal activity either in in vitro assay of microdilution (MIC=3.9 µg/mL) and in vivo model of infection with reduction of S. schenckii load in spleen. It was also observed a predominance of reduction in the production of NO and the proinflammatory cytokines evaluated except TNFα, but with stimulation of IL-10, as evidence of a potential anti-inflammatory effect associated. Conclusion The results showed that both VGL and VGB have a significant antifungal against S. schenckii and an anti-inflammatory activity. These results can support the use of these extracts for alternative treatment of sporotrichosis. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-01-04 2018-12-11T16:45:10Z 2018-12-11T16:45:10Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.jep.2016.11.030 Journal of Ethnopharmacology, v. 195, p. 1-. 1872-7573 0378-8741 http://hdl.handle.net/11449/169276 10.1016/j.jep.2016.11.030 2-s2.0-85007174169 2-s2.0-85007174169.pdf |
url |
http://dx.doi.org/10.1016/j.jep.2016.11.030 http://hdl.handle.net/11449/169276 |
identifier_str_mv |
Journal of Ethnopharmacology, v. 195, p. 1-. 1872-7573 0378-8741 10.1016/j.jep.2016.11.030 2-s2.0-85007174169 2-s2.0-85007174169.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Ethnopharmacology 1,150 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129181638197248 |