Larval gut microbiome of Pelidnota luridipes (Coleoptera: Scarabaeidae): high bacterial diversity, different metabolic profiles on gut chambers and species with probiotic potential
Autor(a) principal: | |
---|---|
Data de Publicação: | 2022 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s11274-022-03387-1 http://hdl.handle.net/11449/241587 |
Resumo: | Pelidnota luridipes Blanchard (1850) is a tropical beetle of the family Scarabaeidae, whose larvae live on wood without parental care. Microbiota of mid- and hindgut of larvae was evaluated by culture-dependent and independent methods, and the results show a diverse microbiota, with most species of bacteria and fungi shared between midgut and hindgut. We isolated 272 bacterial and 29 yeast isolates, identified in 57 and 7 species, respectively, while using metabarcoding, we accessed 1,481 and 267 OTUs of bacteria and fungi, respectively. The composition and abundance of bacteria and fungi differed between mid- and hindgut, with a tendency for higher richness and diversity of yeasts in the midgut, and bacteria on the hindgut. Some taxa are abundant in the intestine of P. luridipes larvae, such as Firmicutes, Bacteroidetes, Proteobacteria, and Actinobacteria; as well as Saccharomycetales and Trichosporonales yeasts. Mid- and hindgut metabolic profiles differ (e.g. biosynthesis of amino acids, cofactors, and lipopolysaccharides) with higher functional diversity in the hindgut. Isolates have different functional traits such as secretion of hydrolytic enzymes and antibiosis against pathogens. Apiotrichum siamense L29A and Bacillus sp. BL17B protected larvae of the moth Galleria mellonella, against infection by the pathogens Listeria monocytogenes ATCC19111 and Pseudomonas aeruginosa ATCC 9027. This is the first work with the larval microbiome of a Rutelini beetle, demonstrating its diversity and potential in prospecting microbial products as probiotics. The functional role of microbiota for the nutrition and adaptability of P. luridipes larvae needs to be evaluated in the future. |
id |
UNSP_164eabb303abb7768f6af186c0763812 |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/241587 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Larval gut microbiome of Pelidnota luridipes (Coleoptera: Scarabaeidae): high bacterial diversity, different metabolic profiles on gut chambers and species with probiotic potentialGrubLytic enzymesMetabarcodingProbioticsRutelinaeSaproxylic beetlesPelidnota luridipes Blanchard (1850) is a tropical beetle of the family Scarabaeidae, whose larvae live on wood without parental care. Microbiota of mid- and hindgut of larvae was evaluated by culture-dependent and independent methods, and the results show a diverse microbiota, with most species of bacteria and fungi shared between midgut and hindgut. We isolated 272 bacterial and 29 yeast isolates, identified in 57 and 7 species, respectively, while using metabarcoding, we accessed 1,481 and 267 OTUs of bacteria and fungi, respectively. The composition and abundance of bacteria and fungi differed between mid- and hindgut, with a tendency for higher richness and diversity of yeasts in the midgut, and bacteria on the hindgut. Some taxa are abundant in the intestine of P. luridipes larvae, such as Firmicutes, Bacteroidetes, Proteobacteria, and Actinobacteria; as well as Saccharomycetales and Trichosporonales yeasts. Mid- and hindgut metabolic profiles differ (e.g. biosynthesis of amino acids, cofactors, and lipopolysaccharides) with higher functional diversity in the hindgut. Isolates have different functional traits such as secretion of hydrolytic enzymes and antibiosis against pathogens. Apiotrichum siamense L29A and Bacillus sp. BL17B protected larvae of the moth Galleria mellonella, against infection by the pathogens Listeria monocytogenes ATCC19111 and Pseudomonas aeruginosa ATCC 9027. This is the first work with the larval microbiome of a Rutelini beetle, demonstrating its diversity and potential in prospecting microbial products as probiotics. The functional role of microbiota for the nutrition and adaptability of P. luridipes larvae needs to be evaluated in the future.Fundação de Amparo à Pesquisa do Estado de Mato GrossoLaboratório de Ecologia e Biotecnologia Microbiana Universidade Federal de Mato Grosso, MTDepartamento de Tecnologia Faculdade de Ciências Agrárias e Veterinárias (FCAV) Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP)Laboratório de Biologia Molecular Embrapa Amazônia Ocidental, AmazonasDepartamento de Tecnologia Faculdade de Ciências Agrárias e Veterinárias (FCAV) Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP)Fundação de Amparo à Pesquisa do Estado de Mato Grosso: 0583867/2016Universidade Federal de Mato GrossoUniversidade Estadual Paulista (UNESP)Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)Falqueto, Silvia Altoéde Sousa, Janaína Rosada Silva, Rafael Correia [UNESP]da Silva, Gilvan FerreiraPinheiro, Daniel Guariz [UNESP]Soares, Marcos Antônio2023-03-01T21:11:36Z2023-03-01T21:11:36Z2022-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1007/s11274-022-03387-1World Journal of Microbiology and Biotechnology, v. 38, n. 11, 2022.1573-09720959-3993http://hdl.handle.net/11449/24158710.1007/s11274-022-03387-12-s2.0-85137117477Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengWorld Journal of Microbiology and Biotechnologyinfo:eu-repo/semantics/openAccess2023-03-01T21:11:36Zoai:repositorio.unesp.br:11449/241587Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-03-01T21:11:36Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Larval gut microbiome of Pelidnota luridipes (Coleoptera: Scarabaeidae): high bacterial diversity, different metabolic profiles on gut chambers and species with probiotic potential |
title |
Larval gut microbiome of Pelidnota luridipes (Coleoptera: Scarabaeidae): high bacterial diversity, different metabolic profiles on gut chambers and species with probiotic potential |
spellingShingle |
Larval gut microbiome of Pelidnota luridipes (Coleoptera: Scarabaeidae): high bacterial diversity, different metabolic profiles on gut chambers and species with probiotic potential Falqueto, Silvia Altoé Grub Lytic enzymes Metabarcoding Probiotics Rutelinae Saproxylic beetles |
title_short |
Larval gut microbiome of Pelidnota luridipes (Coleoptera: Scarabaeidae): high bacterial diversity, different metabolic profiles on gut chambers and species with probiotic potential |
title_full |
Larval gut microbiome of Pelidnota luridipes (Coleoptera: Scarabaeidae): high bacterial diversity, different metabolic profiles on gut chambers and species with probiotic potential |
title_fullStr |
Larval gut microbiome of Pelidnota luridipes (Coleoptera: Scarabaeidae): high bacterial diversity, different metabolic profiles on gut chambers and species with probiotic potential |
title_full_unstemmed |
Larval gut microbiome of Pelidnota luridipes (Coleoptera: Scarabaeidae): high bacterial diversity, different metabolic profiles on gut chambers and species with probiotic potential |
title_sort |
Larval gut microbiome of Pelidnota luridipes (Coleoptera: Scarabaeidae): high bacterial diversity, different metabolic profiles on gut chambers and species with probiotic potential |
author |
Falqueto, Silvia Altoé |
author_facet |
Falqueto, Silvia Altoé de Sousa, Janaína Rosa da Silva, Rafael Correia [UNESP] da Silva, Gilvan Ferreira Pinheiro, Daniel Guariz [UNESP] Soares, Marcos Antônio |
author_role |
author |
author2 |
de Sousa, Janaína Rosa da Silva, Rafael Correia [UNESP] da Silva, Gilvan Ferreira Pinheiro, Daniel Guariz [UNESP] Soares, Marcos Antônio |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de Mato Grosso Universidade Estadual Paulista (UNESP) Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) |
dc.contributor.author.fl_str_mv |
Falqueto, Silvia Altoé de Sousa, Janaína Rosa da Silva, Rafael Correia [UNESP] da Silva, Gilvan Ferreira Pinheiro, Daniel Guariz [UNESP] Soares, Marcos Antônio |
dc.subject.por.fl_str_mv |
Grub Lytic enzymes Metabarcoding Probiotics Rutelinae Saproxylic beetles |
topic |
Grub Lytic enzymes Metabarcoding Probiotics Rutelinae Saproxylic beetles |
description |
Pelidnota luridipes Blanchard (1850) is a tropical beetle of the family Scarabaeidae, whose larvae live on wood without parental care. Microbiota of mid- and hindgut of larvae was evaluated by culture-dependent and independent methods, and the results show a diverse microbiota, with most species of bacteria and fungi shared between midgut and hindgut. We isolated 272 bacterial and 29 yeast isolates, identified in 57 and 7 species, respectively, while using metabarcoding, we accessed 1,481 and 267 OTUs of bacteria and fungi, respectively. The composition and abundance of bacteria and fungi differed between mid- and hindgut, with a tendency for higher richness and diversity of yeasts in the midgut, and bacteria on the hindgut. Some taxa are abundant in the intestine of P. luridipes larvae, such as Firmicutes, Bacteroidetes, Proteobacteria, and Actinobacteria; as well as Saccharomycetales and Trichosporonales yeasts. Mid- and hindgut metabolic profiles differ (e.g. biosynthesis of amino acids, cofactors, and lipopolysaccharides) with higher functional diversity in the hindgut. Isolates have different functional traits such as secretion of hydrolytic enzymes and antibiosis against pathogens. Apiotrichum siamense L29A and Bacillus sp. BL17B protected larvae of the moth Galleria mellonella, against infection by the pathogens Listeria monocytogenes ATCC19111 and Pseudomonas aeruginosa ATCC 9027. This is the first work with the larval microbiome of a Rutelini beetle, demonstrating its diversity and potential in prospecting microbial products as probiotics. The functional role of microbiota for the nutrition and adaptability of P. luridipes larvae needs to be evaluated in the future. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-11-01 2023-03-01T21:11:36Z 2023-03-01T21:11:36Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s11274-022-03387-1 World Journal of Microbiology and Biotechnology, v. 38, n. 11, 2022. 1573-0972 0959-3993 http://hdl.handle.net/11449/241587 10.1007/s11274-022-03387-1 2-s2.0-85137117477 |
url |
http://dx.doi.org/10.1007/s11274-022-03387-1 http://hdl.handle.net/11449/241587 |
identifier_str_mv |
World Journal of Microbiology and Biotechnology, v. 38, n. 11, 2022. 1573-0972 0959-3993 10.1007/s11274-022-03387-1 2-s2.0-85137117477 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
World Journal of Microbiology and Biotechnology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1797789515455135744 |