A Novel Apilic Antivenom to Treat Massive, Africanized Honeybee Attacks: A Preclinical Study from the Lethality to Some Biochemical and Pharmacological Activities Neutralization
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3390/toxins13010030 http://hdl.handle.net/11449/207217 |
Resumo: | Massive, Africanized honeybee attacks have increased in Brazil over the years. Humans and animals present local and systemic effects after envenomation, and there is no specific treatment for this potentially lethal event. This study evaluated the ability of a new Apilic antivenom, which is composed of F(ab')2 fraction of specific immunoglobulins in heterologous and hyperimmune equine serum, to neutralize A. mellifera venom and melittin, in vitro and in vivo, in mice. Animal experiments were performed in according with local ethics committee license (UFRJ protocol no. DFBCICB072-04/16). Venom dose-dependent lethality was diminished with 0.25-0.5 μL of intravenous Apilic antivenom/μg honeybee venom. In vivo injection of 0.1-1 μg/g bee venom induced myotoxicity, hemoconcentration, paw edema, and increase of vascular permeability which were antagonized by Apilic antivenom. Cytotoxicity, assessed in renal LLC-PK1 cells and challenged with 10 μg/mL honeybee venom or melittin, was neutralized by preincubation with Apilic antivenom, as well the hemolytic activity. Apilic antivenom inhibited phospholipase and hyaluronidase enzymatic activities. In flow cytometry experiments, Apilic antivenom neutralized reduction of cell viability due to necrosis by honeybee venom or melittin. These results showed that this antivenom is effective inhibitor of honeybee venom actions. Thus, this next generation of Apilic antivenom emerges as a new promising immunobiological product for the treatment of massive, Africanized honeybee attacks. |
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A Novel Apilic Antivenom to Treat Massive, Africanized Honeybee Attacks: A Preclinical Study from the Lethality to Some Biochemical and Pharmacological Activities NeutralizationApilic antivenomApis melliferaenvenomationhoneybee venommelittinpreclinical studyMassive, Africanized honeybee attacks have increased in Brazil over the years. Humans and animals present local and systemic effects after envenomation, and there is no specific treatment for this potentially lethal event. This study evaluated the ability of a new Apilic antivenom, which is composed of F(ab')2 fraction of specific immunoglobulins in heterologous and hyperimmune equine serum, to neutralize A. mellifera venom and melittin, in vitro and in vivo, in mice. Animal experiments were performed in according with local ethics committee license (UFRJ protocol no. DFBCICB072-04/16). Venom dose-dependent lethality was diminished with 0.25-0.5 μL of intravenous Apilic antivenom/μg honeybee venom. In vivo injection of 0.1-1 μg/g bee venom induced myotoxicity, hemoconcentration, paw edema, and increase of vascular permeability which were antagonized by Apilic antivenom. Cytotoxicity, assessed in renal LLC-PK1 cells and challenged with 10 μg/mL honeybee venom or melittin, was neutralized by preincubation with Apilic antivenom, as well the hemolytic activity. Apilic antivenom inhibited phospholipase and hyaluronidase enzymatic activities. In flow cytometry experiments, Apilic antivenom neutralized reduction of cell viability due to necrosis by honeybee venom or melittin. These results showed that this antivenom is effective inhibitor of honeybee venom actions. Thus, this next generation of Apilic antivenom emerges as a new promising immunobiological product for the treatment of massive, Africanized honeybee attacks.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ)Graduate Program in Pharmacology and Medicinal Chemistry Institute of Biomedical Sciences Federal University of Rio de JaneiroScientific BoardCenter for the Study of Venoms and Venomous Animals (CEVAP) São Paulo State University (UNESP)Center for the Study of Venoms and Venomous Animals (CEVAP) São Paulo State University (UNESP)CAPES: 23038.006285/2011-21CNPq: 401170/2013-6CNPq: 409436/2016-0FAPERJ: e-26/200.006/2018FAPERJ: E_02/2017Federal University of Rio de JaneiroScientific BoardUniversidade Estadual Paulista (Unesp)Teixeira-Cruz, Jhonatha MotaStrauch, Marcelo AbrahãoMonteiro-Machado, MarcosTavares-Henriques, Matheus Silvade Moraes, João AlfredoRibeiro da Cunha, Luís EduardoFerreira, Rui Seabra [UNESP]Barraviera, Benedito [UNESP]Quintas, Luis Eduardo MMelo, Paulo A.2021-06-25T10:50:55Z2021-06-25T10:50:55Z2021-01-05info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3390/toxins13010030Toxins, v. 13, n. 1, 2021.2072-6651http://hdl.handle.net/11449/20721710.3390/toxins130100302-s2.0-85100229954Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengToxinsinfo:eu-repo/semantics/openAccess2024-04-11T15:28:16Zoai:repositorio.unesp.br:11449/207217Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T14:01:17.221134Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
A Novel Apilic Antivenom to Treat Massive, Africanized Honeybee Attacks: A Preclinical Study from the Lethality to Some Biochemical and Pharmacological Activities Neutralization |
title |
A Novel Apilic Antivenom to Treat Massive, Africanized Honeybee Attacks: A Preclinical Study from the Lethality to Some Biochemical and Pharmacological Activities Neutralization |
spellingShingle |
A Novel Apilic Antivenom to Treat Massive, Africanized Honeybee Attacks: A Preclinical Study from the Lethality to Some Biochemical and Pharmacological Activities Neutralization Teixeira-Cruz, Jhonatha Mota Apilic antivenom Apis mellifera envenomation honeybee venom melittin preclinical study |
title_short |
A Novel Apilic Antivenom to Treat Massive, Africanized Honeybee Attacks: A Preclinical Study from the Lethality to Some Biochemical and Pharmacological Activities Neutralization |
title_full |
A Novel Apilic Antivenom to Treat Massive, Africanized Honeybee Attacks: A Preclinical Study from the Lethality to Some Biochemical and Pharmacological Activities Neutralization |
title_fullStr |
A Novel Apilic Antivenom to Treat Massive, Africanized Honeybee Attacks: A Preclinical Study from the Lethality to Some Biochemical and Pharmacological Activities Neutralization |
title_full_unstemmed |
A Novel Apilic Antivenom to Treat Massive, Africanized Honeybee Attacks: A Preclinical Study from the Lethality to Some Biochemical and Pharmacological Activities Neutralization |
title_sort |
A Novel Apilic Antivenom to Treat Massive, Africanized Honeybee Attacks: A Preclinical Study from the Lethality to Some Biochemical and Pharmacological Activities Neutralization |
author |
Teixeira-Cruz, Jhonatha Mota |
author_facet |
Teixeira-Cruz, Jhonatha Mota Strauch, Marcelo Abrahão Monteiro-Machado, Marcos Tavares-Henriques, Matheus Silva de Moraes, João Alfredo Ribeiro da Cunha, Luís Eduardo Ferreira, Rui Seabra [UNESP] Barraviera, Benedito [UNESP] Quintas, Luis Eduardo M Melo, Paulo A. |
author_role |
author |
author2 |
Strauch, Marcelo Abrahão Monteiro-Machado, Marcos Tavares-Henriques, Matheus Silva de Moraes, João Alfredo Ribeiro da Cunha, Luís Eduardo Ferreira, Rui Seabra [UNESP] Barraviera, Benedito [UNESP] Quintas, Luis Eduardo M Melo, Paulo A. |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Federal University of Rio de Janeiro Scientific Board Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Teixeira-Cruz, Jhonatha Mota Strauch, Marcelo Abrahão Monteiro-Machado, Marcos Tavares-Henriques, Matheus Silva de Moraes, João Alfredo Ribeiro da Cunha, Luís Eduardo Ferreira, Rui Seabra [UNESP] Barraviera, Benedito [UNESP] Quintas, Luis Eduardo M Melo, Paulo A. |
dc.subject.por.fl_str_mv |
Apilic antivenom Apis mellifera envenomation honeybee venom melittin preclinical study |
topic |
Apilic antivenom Apis mellifera envenomation honeybee venom melittin preclinical study |
description |
Massive, Africanized honeybee attacks have increased in Brazil over the years. Humans and animals present local and systemic effects after envenomation, and there is no specific treatment for this potentially lethal event. This study evaluated the ability of a new Apilic antivenom, which is composed of F(ab')2 fraction of specific immunoglobulins in heterologous and hyperimmune equine serum, to neutralize A. mellifera venom and melittin, in vitro and in vivo, in mice. Animal experiments were performed in according with local ethics committee license (UFRJ protocol no. DFBCICB072-04/16). Venom dose-dependent lethality was diminished with 0.25-0.5 μL of intravenous Apilic antivenom/μg honeybee venom. In vivo injection of 0.1-1 μg/g bee venom induced myotoxicity, hemoconcentration, paw edema, and increase of vascular permeability which were antagonized by Apilic antivenom. Cytotoxicity, assessed in renal LLC-PK1 cells and challenged with 10 μg/mL honeybee venom or melittin, was neutralized by preincubation with Apilic antivenom, as well the hemolytic activity. Apilic antivenom inhibited phospholipase and hyaluronidase enzymatic activities. In flow cytometry experiments, Apilic antivenom neutralized reduction of cell viability due to necrosis by honeybee venom or melittin. These results showed that this antivenom is effective inhibitor of honeybee venom actions. Thus, this next generation of Apilic antivenom emerges as a new promising immunobiological product for the treatment of massive, Africanized honeybee attacks. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-25T10:50:55Z 2021-06-25T10:50:55Z 2021-01-05 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3390/toxins13010030 Toxins, v. 13, n. 1, 2021. 2072-6651 http://hdl.handle.net/11449/207217 10.3390/toxins13010030 2-s2.0-85100229954 |
url |
http://dx.doi.org/10.3390/toxins13010030 http://hdl.handle.net/11449/207217 |
identifier_str_mv |
Toxins, v. 13, n. 1, 2021. 2072-6651 10.3390/toxins13010030 2-s2.0-85100229954 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Toxins |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808128304081797120 |