A Novel Apilic Antivenom to Treat Massive, Africanized Honeybee Attacks: A Preclinical Study from the Lethality to Some Biochemical and Pharmacological Activities Neutralization

Detalhes bibliográficos
Autor(a) principal: Teixeira-Cruz, Jhonatha Mota
Data de Publicação: 2021
Outros Autores: Strauch, Marcelo Abrahão, Monteiro-Machado, Marcos, Tavares-Henriques, Matheus Silva, de Moraes, João Alfredo, Ribeiro da Cunha, Luís Eduardo, Ferreira, Rui Seabra [UNESP], Barraviera, Benedito [UNESP], Quintas, Luis Eduardo M, Melo, Paulo A.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.3390/toxins13010030
http://hdl.handle.net/11449/207217
Resumo: Massive, Africanized honeybee attacks have increased in Brazil over the years. Humans and animals present local and systemic effects after envenomation, and there is no specific treatment for this potentially lethal event. This study evaluated the ability of a new Apilic antivenom, which is composed of F(ab')2 fraction of specific immunoglobulins in heterologous and hyperimmune equine serum, to neutralize A. mellifera venom and melittin, in vitro and in vivo, in mice. Animal experiments were performed in according with local ethics committee license (UFRJ protocol no. DFBCICB072-04/16). Venom dose-dependent lethality was diminished with 0.25-0.5 μL of intravenous Apilic antivenom/μg honeybee venom. In vivo injection of 0.1-1 μg/g bee venom induced myotoxicity, hemoconcentration, paw edema, and increase of vascular permeability which were antagonized by Apilic antivenom. Cytotoxicity, assessed in renal LLC-PK1 cells and challenged with 10 μg/mL honeybee venom or melittin, was neutralized by preincubation with Apilic antivenom, as well the hemolytic activity. Apilic antivenom inhibited phospholipase and hyaluronidase enzymatic activities. In flow cytometry experiments, Apilic antivenom neutralized reduction of cell viability due to necrosis by honeybee venom or melittin. These results showed that this antivenom is effective inhibitor of honeybee venom actions. Thus, this next generation of Apilic antivenom emerges as a new promising immunobiological product for the treatment of massive, Africanized honeybee attacks.
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spelling A Novel Apilic Antivenom to Treat Massive, Africanized Honeybee Attacks: A Preclinical Study from the Lethality to Some Biochemical and Pharmacological Activities NeutralizationApilic antivenomApis melliferaenvenomationhoneybee venommelittinpreclinical studyMassive, Africanized honeybee attacks have increased in Brazil over the years. Humans and animals present local and systemic effects after envenomation, and there is no specific treatment for this potentially lethal event. This study evaluated the ability of a new Apilic antivenom, which is composed of F(ab')2 fraction of specific immunoglobulins in heterologous and hyperimmune equine serum, to neutralize A. mellifera venom and melittin, in vitro and in vivo, in mice. Animal experiments were performed in according with local ethics committee license (UFRJ protocol no. DFBCICB072-04/16). Venom dose-dependent lethality was diminished with 0.25-0.5 μL of intravenous Apilic antivenom/μg honeybee venom. In vivo injection of 0.1-1 μg/g bee venom induced myotoxicity, hemoconcentration, paw edema, and increase of vascular permeability which were antagonized by Apilic antivenom. Cytotoxicity, assessed in renal LLC-PK1 cells and challenged with 10 μg/mL honeybee venom or melittin, was neutralized by preincubation with Apilic antivenom, as well the hemolytic activity. Apilic antivenom inhibited phospholipase and hyaluronidase enzymatic activities. In flow cytometry experiments, Apilic antivenom neutralized reduction of cell viability due to necrosis by honeybee venom or melittin. These results showed that this antivenom is effective inhibitor of honeybee venom actions. Thus, this next generation of Apilic antivenom emerges as a new promising immunobiological product for the treatment of massive, Africanized honeybee attacks.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ)Graduate Program in Pharmacology and Medicinal Chemistry Institute of Biomedical Sciences Federal University of Rio de JaneiroScientific BoardCenter for the Study of Venoms and Venomous Animals (CEVAP) São Paulo State University (UNESP)Center for the Study of Venoms and Venomous Animals (CEVAP) São Paulo State University (UNESP)CAPES: 23038.006285/2011-21CNPq: 401170/2013-6CNPq: 409436/2016-0FAPERJ: e-26/200.006/2018FAPERJ: E_02/2017Federal University of Rio de JaneiroScientific BoardUniversidade Estadual Paulista (Unesp)Teixeira-Cruz, Jhonatha MotaStrauch, Marcelo AbrahãoMonteiro-Machado, MarcosTavares-Henriques, Matheus Silvade Moraes, João AlfredoRibeiro da Cunha, Luís EduardoFerreira, Rui Seabra [UNESP]Barraviera, Benedito [UNESP]Quintas, Luis Eduardo MMelo, Paulo A.2021-06-25T10:50:55Z2021-06-25T10:50:55Z2021-01-05info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3390/toxins13010030Toxins, v. 13, n. 1, 2021.2072-6651http://hdl.handle.net/11449/20721710.3390/toxins130100302-s2.0-85100229954Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengToxinsinfo:eu-repo/semantics/openAccess2024-04-11T15:28:16Zoai:repositorio.unesp.br:11449/207217Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T14:01:17.221134Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv A Novel Apilic Antivenom to Treat Massive, Africanized Honeybee Attacks: A Preclinical Study from the Lethality to Some Biochemical and Pharmacological Activities Neutralization
title A Novel Apilic Antivenom to Treat Massive, Africanized Honeybee Attacks: A Preclinical Study from the Lethality to Some Biochemical and Pharmacological Activities Neutralization
spellingShingle A Novel Apilic Antivenom to Treat Massive, Africanized Honeybee Attacks: A Preclinical Study from the Lethality to Some Biochemical and Pharmacological Activities Neutralization
Teixeira-Cruz, Jhonatha Mota
Apilic antivenom
Apis mellifera
envenomation
honeybee venom
melittin
preclinical study
title_short A Novel Apilic Antivenom to Treat Massive, Africanized Honeybee Attacks: A Preclinical Study from the Lethality to Some Biochemical and Pharmacological Activities Neutralization
title_full A Novel Apilic Antivenom to Treat Massive, Africanized Honeybee Attacks: A Preclinical Study from the Lethality to Some Biochemical and Pharmacological Activities Neutralization
title_fullStr A Novel Apilic Antivenom to Treat Massive, Africanized Honeybee Attacks: A Preclinical Study from the Lethality to Some Biochemical and Pharmacological Activities Neutralization
title_full_unstemmed A Novel Apilic Antivenom to Treat Massive, Africanized Honeybee Attacks: A Preclinical Study from the Lethality to Some Biochemical and Pharmacological Activities Neutralization
title_sort A Novel Apilic Antivenom to Treat Massive, Africanized Honeybee Attacks: A Preclinical Study from the Lethality to Some Biochemical and Pharmacological Activities Neutralization
author Teixeira-Cruz, Jhonatha Mota
author_facet Teixeira-Cruz, Jhonatha Mota
Strauch, Marcelo Abrahão
Monteiro-Machado, Marcos
Tavares-Henriques, Matheus Silva
de Moraes, João Alfredo
Ribeiro da Cunha, Luís Eduardo
Ferreira, Rui Seabra [UNESP]
Barraviera, Benedito [UNESP]
Quintas, Luis Eduardo M
Melo, Paulo A.
author_role author
author2 Strauch, Marcelo Abrahão
Monteiro-Machado, Marcos
Tavares-Henriques, Matheus Silva
de Moraes, João Alfredo
Ribeiro da Cunha, Luís Eduardo
Ferreira, Rui Seabra [UNESP]
Barraviera, Benedito [UNESP]
Quintas, Luis Eduardo M
Melo, Paulo A.
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Federal University of Rio de Janeiro
Scientific Board
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Teixeira-Cruz, Jhonatha Mota
Strauch, Marcelo Abrahão
Monteiro-Machado, Marcos
Tavares-Henriques, Matheus Silva
de Moraes, João Alfredo
Ribeiro da Cunha, Luís Eduardo
Ferreira, Rui Seabra [UNESP]
Barraviera, Benedito [UNESP]
Quintas, Luis Eduardo M
Melo, Paulo A.
dc.subject.por.fl_str_mv Apilic antivenom
Apis mellifera
envenomation
honeybee venom
melittin
preclinical study
topic Apilic antivenom
Apis mellifera
envenomation
honeybee venom
melittin
preclinical study
description Massive, Africanized honeybee attacks have increased in Brazil over the years. Humans and animals present local and systemic effects after envenomation, and there is no specific treatment for this potentially lethal event. This study evaluated the ability of a new Apilic antivenom, which is composed of F(ab')2 fraction of specific immunoglobulins in heterologous and hyperimmune equine serum, to neutralize A. mellifera venom and melittin, in vitro and in vivo, in mice. Animal experiments were performed in according with local ethics committee license (UFRJ protocol no. DFBCICB072-04/16). Venom dose-dependent lethality was diminished with 0.25-0.5 μL of intravenous Apilic antivenom/μg honeybee venom. In vivo injection of 0.1-1 μg/g bee venom induced myotoxicity, hemoconcentration, paw edema, and increase of vascular permeability which were antagonized by Apilic antivenom. Cytotoxicity, assessed in renal LLC-PK1 cells and challenged with 10 μg/mL honeybee venom or melittin, was neutralized by preincubation with Apilic antivenom, as well the hemolytic activity. Apilic antivenom inhibited phospholipase and hyaluronidase enzymatic activities. In flow cytometry experiments, Apilic antivenom neutralized reduction of cell viability due to necrosis by honeybee venom or melittin. These results showed that this antivenom is effective inhibitor of honeybee venom actions. Thus, this next generation of Apilic antivenom emerges as a new promising immunobiological product for the treatment of massive, Africanized honeybee attacks.
publishDate 2021
dc.date.none.fl_str_mv 2021-06-25T10:50:55Z
2021-06-25T10:50:55Z
2021-01-05
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.3390/toxins13010030
Toxins, v. 13, n. 1, 2021.
2072-6651
http://hdl.handle.net/11449/207217
10.3390/toxins13010030
2-s2.0-85100229954
url http://dx.doi.org/10.3390/toxins13010030
http://hdl.handle.net/11449/207217
identifier_str_mv Toxins, v. 13, n. 1, 2021.
2072-6651
10.3390/toxins13010030
2-s2.0-85100229954
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Toxins
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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