Microwave-induced fast decalcification of rat bone for electron microscopic analysis: An ultrastructural and cytochemical study
Autor(a) principal: | |
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Data de Publicação: | 2007 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S0103-64402007000200013 http://hdl.handle.net/11449/70066 |
Resumo: | Bone decalcification is a time-consuming process. It takes weeks and preservation of the tissue structure depends on the quality and velocity of the demineralization process. In the present study, a decalcification methodology was adapted using microwaving to accelerate the decalcification of rat bone for electron microscopic analysis. The ultrastructure of the bone decalcified by microwave energy was observed. Wistar rats were perfused with paraformaldehyde and maxillary segments were removed and fixed in glutaraldehyde. Half of specimens were decalcified by conventional treatment with immersion in Warshawsky solution at 4oC during 45 days, and the other half of specimens were placed into the beaker with 20 mL of the Warshawsky solution in ice bath and thereafter submitted to irradiation in a domestic microwave oven (700 maximum power) during 20 s/350 W/±37°C. In the first day, the specimens were irradiated 9 times and stored at 40°C overnight. In the second day, the specimens were irradiated 20 times changing the solution and the ice after each bath. After decalcification, some specimens were postfixed in osmium tetroxide and others in osmium tetroxide and potassium pyroantimonate. The specimens were observed under transmission electron microscopy. The results showed an increase in the decalcification rate in the specimens activated by microwaving and a reduction of total experiment time from 45 days in the conventional method to 48 hours in the microwave-aided method. |
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Microwave-induced fast decalcification of rat bone for electron microscopic analysis: An ultrastructural and cytochemical studyBoneDecalcificationMaxillaMicrowavesPyroantimonateUltrastructurecalciumchelating agentcollagenedetic acidfixativeglutaraldehydesodium hydroxideanimalbonebone matrixcell organellecoldcomparative studycrystallographyhistologylaboratory diagnosismaxillamethodologymicrowave radiationosteoclastosteocyteradiation exposurerattimetransmission electron microscopyultrastructureWistar ratAnimalsBone and BonesBone MatrixCalciumChelating AgentsCold TemperatureCollagenCrystallographyDecalcification TechniqueEdetic AcidFixativesGlutaralMicroscopy, Electron, TransmissionOrganellesOsteoclastsOsteocytesRatsRats, WistarSodium HydroxideSpecimen HandlingTime FactorsBone decalcification is a time-consuming process. It takes weeks and preservation of the tissue structure depends on the quality and velocity of the demineralization process. In the present study, a decalcification methodology was adapted using microwaving to accelerate the decalcification of rat bone for electron microscopic analysis. The ultrastructure of the bone decalcified by microwave energy was observed. Wistar rats were perfused with paraformaldehyde and maxillary segments were removed and fixed in glutaraldehyde. Half of specimens were decalcified by conventional treatment with immersion in Warshawsky solution at 4oC during 45 days, and the other half of specimens were placed into the beaker with 20 mL of the Warshawsky solution in ice bath and thereafter submitted to irradiation in a domestic microwave oven (700 maximum power) during 20 s/350 W/±37°C. In the first day, the specimens were irradiated 9 times and stored at 40°C overnight. In the second day, the specimens were irradiated 20 times changing the solution and the ice after each bath. After decalcification, some specimens were postfixed in osmium tetroxide and others in osmium tetroxide and potassium pyroantimonate. The specimens were observed under transmission electron microscopy. The results showed an increase in the decalcification rate in the specimens activated by microwaving and a reduction of total experiment time from 45 days in the conventional method to 48 hours in the microwave-aided method.Department of Morphology, Stomatology and Physiology School of Dentistry of Ribeirão Preto University of São Paulo, Ribeirão Preto, SPDepartment of Biology Institute of Biosciences São Paulo State University, Rio Claro, SPDepartment of Biology Institute of Biosciences São Paulo State University, Rio Claro, SPUniversidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)Pitol, Dimitrius LeonardoCaetano, Flavio Henrique [UNESP]Lunardi, Laurelúcia Orive [UNESP]2014-05-27T11:22:41Z2014-05-27T11:22:41Z2007-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article153-157application/pdfhttp://dx.doi.org/10.1590/S0103-64402007000200013Brazilian Dental Journal, v. 18, n. 2, p. 153-157, 2007.0103-64401806-4760http://hdl.handle.net/11449/7006610.1590/S0103-64402007000200013S0103-644020070002000132-s2.0-596490924692-s2.0-59649092469.pdf4396826019535898Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Dental Journal0,476info:eu-repo/semantics/openAccess2023-10-11T06:04:33Zoai:repositorio.unesp.br:11449/70066Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T14:36:09.429653Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Microwave-induced fast decalcification of rat bone for electron microscopic analysis: An ultrastructural and cytochemical study |
title |
Microwave-induced fast decalcification of rat bone for electron microscopic analysis: An ultrastructural and cytochemical study |
spellingShingle |
Microwave-induced fast decalcification of rat bone for electron microscopic analysis: An ultrastructural and cytochemical study Pitol, Dimitrius Leonardo Bone Decalcification Maxilla Microwaves Pyroantimonate Ultrastructure calcium chelating agent collagen edetic acid fixative glutaraldehyde sodium hydroxide animal bone bone matrix cell organelle cold comparative study crystallography histology laboratory diagnosis maxilla methodology microwave radiation osteoclast osteocyte radiation exposure rat time transmission electron microscopy ultrastructure Wistar rat Animals Bone and Bones Bone Matrix Calcium Chelating Agents Cold Temperature Collagen Crystallography Decalcification Technique Edetic Acid Fixatives Glutaral Microscopy, Electron, Transmission Organelles Osteoclasts Osteocytes Rats Rats, Wistar Sodium Hydroxide Specimen Handling Time Factors |
title_short |
Microwave-induced fast decalcification of rat bone for electron microscopic analysis: An ultrastructural and cytochemical study |
title_full |
Microwave-induced fast decalcification of rat bone for electron microscopic analysis: An ultrastructural and cytochemical study |
title_fullStr |
Microwave-induced fast decalcification of rat bone for electron microscopic analysis: An ultrastructural and cytochemical study |
title_full_unstemmed |
Microwave-induced fast decalcification of rat bone for electron microscopic analysis: An ultrastructural and cytochemical study |
title_sort |
Microwave-induced fast decalcification of rat bone for electron microscopic analysis: An ultrastructural and cytochemical study |
author |
Pitol, Dimitrius Leonardo |
author_facet |
Pitol, Dimitrius Leonardo Caetano, Flavio Henrique [UNESP] Lunardi, Laurelúcia Orive [UNESP] |
author_role |
author |
author2 |
Caetano, Flavio Henrique [UNESP] Lunardi, Laurelúcia Orive [UNESP] |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Pitol, Dimitrius Leonardo Caetano, Flavio Henrique [UNESP] Lunardi, Laurelúcia Orive [UNESP] |
dc.subject.por.fl_str_mv |
Bone Decalcification Maxilla Microwaves Pyroantimonate Ultrastructure calcium chelating agent collagen edetic acid fixative glutaraldehyde sodium hydroxide animal bone bone matrix cell organelle cold comparative study crystallography histology laboratory diagnosis maxilla methodology microwave radiation osteoclast osteocyte radiation exposure rat time transmission electron microscopy ultrastructure Wistar rat Animals Bone and Bones Bone Matrix Calcium Chelating Agents Cold Temperature Collagen Crystallography Decalcification Technique Edetic Acid Fixatives Glutaral Microscopy, Electron, Transmission Organelles Osteoclasts Osteocytes Rats Rats, Wistar Sodium Hydroxide Specimen Handling Time Factors |
topic |
Bone Decalcification Maxilla Microwaves Pyroantimonate Ultrastructure calcium chelating agent collagen edetic acid fixative glutaraldehyde sodium hydroxide animal bone bone matrix cell organelle cold comparative study crystallography histology laboratory diagnosis maxilla methodology microwave radiation osteoclast osteocyte radiation exposure rat time transmission electron microscopy ultrastructure Wistar rat Animals Bone and Bones Bone Matrix Calcium Chelating Agents Cold Temperature Collagen Crystallography Decalcification Technique Edetic Acid Fixatives Glutaral Microscopy, Electron, Transmission Organelles Osteoclasts Osteocytes Rats Rats, Wistar Sodium Hydroxide Specimen Handling Time Factors |
description |
Bone decalcification is a time-consuming process. It takes weeks and preservation of the tissue structure depends on the quality and velocity of the demineralization process. In the present study, a decalcification methodology was adapted using microwaving to accelerate the decalcification of rat bone for electron microscopic analysis. The ultrastructure of the bone decalcified by microwave energy was observed. Wistar rats were perfused with paraformaldehyde and maxillary segments were removed and fixed in glutaraldehyde. Half of specimens were decalcified by conventional treatment with immersion in Warshawsky solution at 4oC during 45 days, and the other half of specimens were placed into the beaker with 20 mL of the Warshawsky solution in ice bath and thereafter submitted to irradiation in a domestic microwave oven (700 maximum power) during 20 s/350 W/±37°C. In the first day, the specimens were irradiated 9 times and stored at 40°C overnight. In the second day, the specimens were irradiated 20 times changing the solution and the ice after each bath. After decalcification, some specimens were postfixed in osmium tetroxide and others in osmium tetroxide and potassium pyroantimonate. The specimens were observed under transmission electron microscopy. The results showed an increase in the decalcification rate in the specimens activated by microwaving and a reduction of total experiment time from 45 days in the conventional method to 48 hours in the microwave-aided method. |
publishDate |
2007 |
dc.date.none.fl_str_mv |
2007-12-01 2014-05-27T11:22:41Z 2014-05-27T11:22:41Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S0103-64402007000200013 Brazilian Dental Journal, v. 18, n. 2, p. 153-157, 2007. 0103-6440 1806-4760 http://hdl.handle.net/11449/70066 10.1590/S0103-64402007000200013 S0103-64402007000200013 2-s2.0-59649092469 2-s2.0-59649092469.pdf 4396826019535898 |
url |
http://dx.doi.org/10.1590/S0103-64402007000200013 http://hdl.handle.net/11449/70066 |
identifier_str_mv |
Brazilian Dental Journal, v. 18, n. 2, p. 153-157, 2007. 0103-6440 1806-4760 10.1590/S0103-64402007000200013 S0103-64402007000200013 2-s2.0-59649092469 2-s2.0-59649092469.pdf 4396826019535898 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Brazilian Dental Journal 0,476 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
153-157 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128385579220992 |