Sequenciamento do genoma completo do vírus Culex flavivirus (Flaviviridae) isolado no Brasil

Detalhes bibliográficos
Autor(a) principal: Machado, Daiane Cristina [UNESP]
Data de Publicação: 2014
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://hdl.handle.net/11449/154714
http://www.athena.biblioteca.unesp.br/exlibris/bd/cathedra/02-08-2017/000869213.pdf
Resumo: In Brazil, the presence of ecosystems with a wealth and diversity of animals, and cities large and densely populated offers conditions for the occurrence of many arboviruses. Several arboviruses transmitted by mosquitoes of the genus Culex belong to the genus Flavivirus. Viruses of this genus are known to cause disease in humans and animals, however, flaviviruses do not infect or cause disease in vertebrates, replicating only in arthropods have been described. Culex flavivirus virus is an example. He was isolated from species of mosquitoes of the genus Culex in North America, Central America, South America, Africa and Asia. Although studies conducted in other parts of the world, in Brazil there is still little information about this virus. In this work we performed the sequencing of the complete genome of Culex flavivirus virus (CxFV) isolated from Culex mosquitoes in São José do Rio Preto (SP) using the techniques of RT-PCR and nucleotide sequencing. The viral genome of the isolated (CxFV_RPBR07_2007) obtained in the present study presented 10.706 nucleotides with an ORF of 10.092 nucleotides (3.364 amino acids) flanked by a 5'UTR of 32 nucleotides and a 3'UTR of 582 nucleotides. Phylogenetic analyzes revealed that the CxFV_RPBR07_2007 groups with other insect flaviviruses such as the Cell fusing agent virus (CFAV) and Kamiti River virus (KRV), and is closely related to CxFV isolates from Mexico (Latin America) and Uganda (Africa); also maintaining relationship with isolates from the United States of America (North America) and Japan (Asia). These groupings were also observed in other phylogenetic studies
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spelling Sequenciamento do genoma completo do vírus Culex flavivirus (Flaviviridae) isolado no BrasilVirologyVirologiaSeqüenciamento de nucleotídeoGenomaCulex - São José do Rio Preto (SP)Flavivírus - São José do Rio Preto (SP)In Brazil, the presence of ecosystems with a wealth and diversity of animals, and cities large and densely populated offers conditions for the occurrence of many arboviruses. Several arboviruses transmitted by mosquitoes of the genus Culex belong to the genus Flavivirus. Viruses of this genus are known to cause disease in humans and animals, however, flaviviruses do not infect or cause disease in vertebrates, replicating only in arthropods have been described. Culex flavivirus virus is an example. He was isolated from species of mosquitoes of the genus Culex in North America, Central America, South America, Africa and Asia. Although studies conducted in other parts of the world, in Brazil there is still little information about this virus. In this work we performed the sequencing of the complete genome of Culex flavivirus virus (CxFV) isolated from Culex mosquitoes in São José do Rio Preto (SP) using the techniques of RT-PCR and nucleotide sequencing. The viral genome of the isolated (CxFV_RPBR07_2007) obtained in the present study presented 10.706 nucleotides with an ORF of 10.092 nucleotides (3.364 amino acids) flanked by a 5'UTR of 32 nucleotides and a 3'UTR of 582 nucleotides. Phylogenetic analyzes revealed that the CxFV_RPBR07_2007 groups with other insect flaviviruses such as the Cell fusing agent virus (CFAV) and Kamiti River virus (KRV), and is closely related to CxFV isolates from Mexico (Latin America) and Uganda (Africa); also maintaining relationship with isolates from the United States of America (North America) and Japan (Asia). These groupings were also observed in other phylogenetic studiesNo Brasil, a presença de ecossistemas com uma grande riqueza e diversidade de animais e cidades grandes e densamente povoadas oferece condições para a ocorrência de diversas arboviroses. Vários arbovírus transmitidos por mosquitos do gênero Culex pertencem ao gênero Flavivirus. Vírus desse gênero são conhecidos por causar doenças em humanos e animais, no entanto, flavivírus que não infectam ou causam doença em vertebrados, replicando somente em artrópodes têm sido descritos. O vírus Culex flavivirus é um exemplo. Ele foi isolado de espécies de mosquitos do gênero Culex na América do Norte, América Central, América do Sul, África e Ásia. Apesar dos estudos realizados em outras partes do mundo, no Brasil há ainda pouca informação sobre esse vírus. Nesse trabalho, realizamos o seqüenciamento do genoma completo do vírus Culex flavivirus (CxFV) isolado de mosquitos Culex de São José do Rio Preto (SP), por meio das técnicas de RT-PCR e seqüenciamento nucleotídico. O genoma viral do isolado CxFV_RPBR07_2007, o qual foi obtido no presente estudo apresentou 10.706 nucleotídeos com uma ORF de 10.092 nucleotídeos (3.364 aminoácidos) flanqueada por uma 5'UTR de 32 nucleotídeos e uma 3'UTR de 582 nucleotídeos. Análises filogenéticas revelaram que o CxFV_RPBR07_2007 agrupa-se com outros flavivírus de insetos, como o Cell fusing agent virus (CFAV) e o Kamiti River virus (KRV), e relaciona-se intimamente com isolados de CxFV do México (América Latina) e de Uganda (África); mantendo relação também com isolados dos Estados Unidos (América do Norte) e do Japão (Ásia). Esses agrupamentos foram também observados em outros estudos filogenéticosCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Universidade Estadual Paulista (Unesp)Nogueira, Maurício Lacerda [UNESP]Universidade Estadual Paulista (Unesp)Machado, Daiane Cristina [UNESP]2018-07-27T18:26:11Z2018-07-27T18:26:11Z2014-10-24info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis45 f. : il. color.application/pdfMACHADO, Daiane Cristina. Sequenciamento do genoma completo do vírus Culex flavivirus (Flaviviridae) isolado no Brasil. 2016. 45 f. Dissertação (mestrado) - Universidade Estadual Paulista Julio de Mesquita Filho, Instituto de Biociências, Letras e Ciências Exatas, 2016.http://hdl.handle.net/11449/154714000869213http://www.athena.biblioteca.unesp.br/exlibris/bd/cathedra/02-08-2017/000869213.pdf33004153074P9Alephreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPporinfo:eu-repo/semantics/openAccess2023-10-30T06:07:48Zoai:repositorio.unesp.br:11449/154714Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T16:26:10.437562Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Sequenciamento do genoma completo do vírus Culex flavivirus (Flaviviridae) isolado no Brasil
title Sequenciamento do genoma completo do vírus Culex flavivirus (Flaviviridae) isolado no Brasil
spellingShingle Sequenciamento do genoma completo do vírus Culex flavivirus (Flaviviridae) isolado no Brasil
Machado, Daiane Cristina [UNESP]
Virology
Virologia
Seqüenciamento de nucleotídeo
Genoma
Culex - São José do Rio Preto (SP)
Flavivírus - São José do Rio Preto (SP)
title_short Sequenciamento do genoma completo do vírus Culex flavivirus (Flaviviridae) isolado no Brasil
title_full Sequenciamento do genoma completo do vírus Culex flavivirus (Flaviviridae) isolado no Brasil
title_fullStr Sequenciamento do genoma completo do vírus Culex flavivirus (Flaviviridae) isolado no Brasil
title_full_unstemmed Sequenciamento do genoma completo do vírus Culex flavivirus (Flaviviridae) isolado no Brasil
title_sort Sequenciamento do genoma completo do vírus Culex flavivirus (Flaviviridae) isolado no Brasil
author Machado, Daiane Cristina [UNESP]
author_facet Machado, Daiane Cristina [UNESP]
author_role author
dc.contributor.none.fl_str_mv Nogueira, Maurício Lacerda [UNESP]
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Machado, Daiane Cristina [UNESP]
dc.subject.por.fl_str_mv Virology
Virologia
Seqüenciamento de nucleotídeo
Genoma
Culex - São José do Rio Preto (SP)
Flavivírus - São José do Rio Preto (SP)
topic Virology
Virologia
Seqüenciamento de nucleotídeo
Genoma
Culex - São José do Rio Preto (SP)
Flavivírus - São José do Rio Preto (SP)
description In Brazil, the presence of ecosystems with a wealth and diversity of animals, and cities large and densely populated offers conditions for the occurrence of many arboviruses. Several arboviruses transmitted by mosquitoes of the genus Culex belong to the genus Flavivirus. Viruses of this genus are known to cause disease in humans and animals, however, flaviviruses do not infect or cause disease in vertebrates, replicating only in arthropods have been described. Culex flavivirus virus is an example. He was isolated from species of mosquitoes of the genus Culex in North America, Central America, South America, Africa and Asia. Although studies conducted in other parts of the world, in Brazil there is still little information about this virus. In this work we performed the sequencing of the complete genome of Culex flavivirus virus (CxFV) isolated from Culex mosquitoes in São José do Rio Preto (SP) using the techniques of RT-PCR and nucleotide sequencing. The viral genome of the isolated (CxFV_RPBR07_2007) obtained in the present study presented 10.706 nucleotides with an ORF of 10.092 nucleotides (3.364 amino acids) flanked by a 5'UTR of 32 nucleotides and a 3'UTR of 582 nucleotides. Phylogenetic analyzes revealed that the CxFV_RPBR07_2007 groups with other insect flaviviruses such as the Cell fusing agent virus (CFAV) and Kamiti River virus (KRV), and is closely related to CxFV isolates from Mexico (Latin America) and Uganda (Africa); also maintaining relationship with isolates from the United States of America (North America) and Japan (Asia). These groupings were also observed in other phylogenetic studies
publishDate 2014
dc.date.none.fl_str_mv 2014-10-24
2018-07-27T18:26:11Z
2018-07-27T18:26:11Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv MACHADO, Daiane Cristina. Sequenciamento do genoma completo do vírus Culex flavivirus (Flaviviridae) isolado no Brasil. 2016. 45 f. Dissertação (mestrado) - Universidade Estadual Paulista Julio de Mesquita Filho, Instituto de Biociências, Letras e Ciências Exatas, 2016.
http://hdl.handle.net/11449/154714
000869213
http://www.athena.biblioteca.unesp.br/exlibris/bd/cathedra/02-08-2017/000869213.pdf
33004153074P9
identifier_str_mv MACHADO, Daiane Cristina. Sequenciamento do genoma completo do vírus Culex flavivirus (Flaviviridae) isolado no Brasil. 2016. 45 f. Dissertação (mestrado) - Universidade Estadual Paulista Julio de Mesquita Filho, Instituto de Biociências, Letras e Ciências Exatas, 2016.
000869213
33004153074P9
url http://hdl.handle.net/11449/154714
http://www.athena.biblioteca.unesp.br/exlibris/bd/cathedra/02-08-2017/000869213.pdf
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dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.format.none.fl_str_mv 45 f. : il. color.
application/pdf
dc.publisher.none.fl_str_mv Universidade Estadual Paulista (Unesp)
publisher.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.source.none.fl_str_mv Aleph
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
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instname_str Universidade Estadual Paulista (UNESP)
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institution UNESP
reponame_str Repositório Institucional da UNESP
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repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
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