Minimum Lignin and Xylan Removal to Improve Cellulose Accessibility
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s12155-020-10120-z http://hdl.handle.net/11449/200289 |
Resumo: | The lignocellulosic biomass, such as provided by the sugarcane, is an abundant source of raw materials for energy production. Milling and pretreatments can be employed to alter the structure of the materials, remove lignin, and hemicellulose. This pretreatment effect exposes the cellulose and raises its accessibility, which is one of the most important properties to ensure enzymatic digestibility. However, the biomass generated from the sugarcane has different physicochemical characteristics, giving different responses to the pretreatments. In this context, this study aimed to verify the effects of lignin and hemicellulose removal from the sugarcane biomass (external fraction, node, internode, and leaf) on cellulose accessibility. Each fraction was pretreated with acid (5, 10, and 20% w/w, at 121 °C/30 min), alkali (5, 10, 20, and 30% NaOH w/w) and oxidative (0.5, 1, 2, and 3 h charged with 30% sodium chlorite). Accessibility was determined by dye adsorption of Direct Orange (external specific surface) and Direct Blue (internal specific surface). Enzymatic hydrolysis was used to verify the effects of pretreatments and cellulose accessibility on the glucose yield. Delignification by sodium chlorite (oxidative) resulted in lignin removal, with almost complete removal from leaf samples. Accessibility determined indicated that pretreatments that are more aggressive improved cellulose accessibility. The less recalcitrant fraction, the internode, showed 1333.3 mg/g of Direct Orange adsorbed and 746.3 mg/g of Direct Blue. Glucose yield during enzymatic hydrolysis improved with higher cellulose accessibility. Lignin and xylan removal (down to 10% and 1%, respectively) resulted in higher glucose yield, with delignified internode samples showing almost complete cellulose conversion. Hemicellulose and lignin removal by the pretreatments directly influenced cellulose accessibility, resulting in better enzymatic hydrolysis across all fractions. This study successfully showed that lignin and hemicellulose removal of 15% and 10%, respectively, resulting in at least 60% of glucose yield, reaching desired accessibility levels based on dye adsorption of 2079.6 mg/g. |
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Minimum Lignin and Xylan Removal to Improve Cellulose AccessibilityAccessibilityAlkaline pretreatmentDiluted acid pretreatmentEnzymatic hydrolysisOxidative pretreatmentThe lignocellulosic biomass, such as provided by the sugarcane, is an abundant source of raw materials for energy production. Milling and pretreatments can be employed to alter the structure of the materials, remove lignin, and hemicellulose. This pretreatment effect exposes the cellulose and raises its accessibility, which is one of the most important properties to ensure enzymatic digestibility. However, the biomass generated from the sugarcane has different physicochemical characteristics, giving different responses to the pretreatments. In this context, this study aimed to verify the effects of lignin and hemicellulose removal from the sugarcane biomass (external fraction, node, internode, and leaf) on cellulose accessibility. Each fraction was pretreated with acid (5, 10, and 20% w/w, at 121 °C/30 min), alkali (5, 10, 20, and 30% NaOH w/w) and oxidative (0.5, 1, 2, and 3 h charged with 30% sodium chlorite). Accessibility was determined by dye adsorption of Direct Orange (external specific surface) and Direct Blue (internal specific surface). Enzymatic hydrolysis was used to verify the effects of pretreatments and cellulose accessibility on the glucose yield. Delignification by sodium chlorite (oxidative) resulted in lignin removal, with almost complete removal from leaf samples. Accessibility determined indicated that pretreatments that are more aggressive improved cellulose accessibility. The less recalcitrant fraction, the internode, showed 1333.3 mg/g of Direct Orange adsorbed and 746.3 mg/g of Direct Blue. Glucose yield during enzymatic hydrolysis improved with higher cellulose accessibility. Lignin and xylan removal (down to 10% and 1%, respectively) resulted in higher glucose yield, with delignified internode samples showing almost complete cellulose conversion. Hemicellulose and lignin removal by the pretreatments directly influenced cellulose accessibility, resulting in better enzymatic hydrolysis across all fractions. This study successfully showed that lignin and hemicellulose removal of 15% and 10%, respectively, resulting in at least 60% of glucose yield, reaching desired accessibility levels based on dye adsorption of 2079.6 mg/g.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Bioenergy Research Institute (IPBEN) Universidade Estadual Paulista (UNESP)Biochemistry and Microbiology Department Universidade Estadual Paulista (UNESP)Bioenergy Research Institute (IPBEN) Universidade Estadual Paulista (UNESP)Biochemistry and Microbiology Department Universidade Estadual Paulista (UNESP)CNPq: 401900/2016-9Universidade Estadual Paulista (Unesp)Shimizu, Felipe Lange [UNESP]de Azevedo, Gabriel Oliveira [UNESP]Coelho, Luciana Fontes [UNESP]Pagnocca, Fernando Carlos [UNESP]Brienzo, Michel [UNESP]2020-12-12T02:02:44Z2020-12-12T02:02:44Z2020-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article775-785http://dx.doi.org/10.1007/s12155-020-10120-zBioenergy Research, v. 13, n. 3, p. 775-785, 2020.1939-12421939-1234http://hdl.handle.net/11449/20028910.1007/s12155-020-10120-z2-s2.0-850833731868251885707409794Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBioenergy Researchinfo:eu-repo/semantics/openAccess2022-03-14T22:05:50Zoai:repositorio.unesp.br:11449/200289Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:24:48.563592Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Minimum Lignin and Xylan Removal to Improve Cellulose Accessibility |
title |
Minimum Lignin and Xylan Removal to Improve Cellulose Accessibility |
spellingShingle |
Minimum Lignin and Xylan Removal to Improve Cellulose Accessibility Shimizu, Felipe Lange [UNESP] Accessibility Alkaline pretreatment Diluted acid pretreatment Enzymatic hydrolysis Oxidative pretreatment |
title_short |
Minimum Lignin and Xylan Removal to Improve Cellulose Accessibility |
title_full |
Minimum Lignin and Xylan Removal to Improve Cellulose Accessibility |
title_fullStr |
Minimum Lignin and Xylan Removal to Improve Cellulose Accessibility |
title_full_unstemmed |
Minimum Lignin and Xylan Removal to Improve Cellulose Accessibility |
title_sort |
Minimum Lignin and Xylan Removal to Improve Cellulose Accessibility |
author |
Shimizu, Felipe Lange [UNESP] |
author_facet |
Shimizu, Felipe Lange [UNESP] de Azevedo, Gabriel Oliveira [UNESP] Coelho, Luciana Fontes [UNESP] Pagnocca, Fernando Carlos [UNESP] Brienzo, Michel [UNESP] |
author_role |
author |
author2 |
de Azevedo, Gabriel Oliveira [UNESP] Coelho, Luciana Fontes [UNESP] Pagnocca, Fernando Carlos [UNESP] Brienzo, Michel [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Shimizu, Felipe Lange [UNESP] de Azevedo, Gabriel Oliveira [UNESP] Coelho, Luciana Fontes [UNESP] Pagnocca, Fernando Carlos [UNESP] Brienzo, Michel [UNESP] |
dc.subject.por.fl_str_mv |
Accessibility Alkaline pretreatment Diluted acid pretreatment Enzymatic hydrolysis Oxidative pretreatment |
topic |
Accessibility Alkaline pretreatment Diluted acid pretreatment Enzymatic hydrolysis Oxidative pretreatment |
description |
The lignocellulosic biomass, such as provided by the sugarcane, is an abundant source of raw materials for energy production. Milling and pretreatments can be employed to alter the structure of the materials, remove lignin, and hemicellulose. This pretreatment effect exposes the cellulose and raises its accessibility, which is one of the most important properties to ensure enzymatic digestibility. However, the biomass generated from the sugarcane has different physicochemical characteristics, giving different responses to the pretreatments. In this context, this study aimed to verify the effects of lignin and hemicellulose removal from the sugarcane biomass (external fraction, node, internode, and leaf) on cellulose accessibility. Each fraction was pretreated with acid (5, 10, and 20% w/w, at 121 °C/30 min), alkali (5, 10, 20, and 30% NaOH w/w) and oxidative (0.5, 1, 2, and 3 h charged with 30% sodium chlorite). Accessibility was determined by dye adsorption of Direct Orange (external specific surface) and Direct Blue (internal specific surface). Enzymatic hydrolysis was used to verify the effects of pretreatments and cellulose accessibility on the glucose yield. Delignification by sodium chlorite (oxidative) resulted in lignin removal, with almost complete removal from leaf samples. Accessibility determined indicated that pretreatments that are more aggressive improved cellulose accessibility. The less recalcitrant fraction, the internode, showed 1333.3 mg/g of Direct Orange adsorbed and 746.3 mg/g of Direct Blue. Glucose yield during enzymatic hydrolysis improved with higher cellulose accessibility. Lignin and xylan removal (down to 10% and 1%, respectively) resulted in higher glucose yield, with delignified internode samples showing almost complete cellulose conversion. Hemicellulose and lignin removal by the pretreatments directly influenced cellulose accessibility, resulting in better enzymatic hydrolysis across all fractions. This study successfully showed that lignin and hemicellulose removal of 15% and 10%, respectively, resulting in at least 60% of glucose yield, reaching desired accessibility levels based on dye adsorption of 2079.6 mg/g. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T02:02:44Z 2020-12-12T02:02:44Z 2020-09-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s12155-020-10120-z Bioenergy Research, v. 13, n. 3, p. 775-785, 2020. 1939-1242 1939-1234 http://hdl.handle.net/11449/200289 10.1007/s12155-020-10120-z 2-s2.0-85083373186 8251885707409794 |
url |
http://dx.doi.org/10.1007/s12155-020-10120-z http://hdl.handle.net/11449/200289 |
identifier_str_mv |
Bioenergy Research, v. 13, n. 3, p. 775-785, 2020. 1939-1242 1939-1234 10.1007/s12155-020-10120-z 2-s2.0-85083373186 8251885707409794 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Bioenergy Research |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
775-785 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128806991429632 |