Minimum Lignin and Xylan Removal to Improve Cellulose Accessibility

Detalhes bibliográficos
Autor(a) principal: Shimizu, Felipe Lange [UNESP]
Data de Publicação: 2020
Outros Autores: de Azevedo, Gabriel Oliveira [UNESP], Coelho, Luciana Fontes [UNESP], Pagnocca, Fernando Carlos [UNESP], Brienzo, Michel [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1007/s12155-020-10120-z
http://hdl.handle.net/11449/200289
Resumo: The lignocellulosic biomass, such as provided by the sugarcane, is an abundant source of raw materials for energy production. Milling and pretreatments can be employed to alter the structure of the materials, remove lignin, and hemicellulose. This pretreatment effect exposes the cellulose and raises its accessibility, which is one of the most important properties to ensure enzymatic digestibility. However, the biomass generated from the sugarcane has different physicochemical characteristics, giving different responses to the pretreatments. In this context, this study aimed to verify the effects of lignin and hemicellulose removal from the sugarcane biomass (external fraction, node, internode, and leaf) on cellulose accessibility. Each fraction was pretreated with acid (5, 10, and 20% w/w, at 121 °C/30 min), alkali (5, 10, 20, and 30% NaOH w/w) and oxidative (0.5, 1, 2, and 3 h charged with 30% sodium chlorite). Accessibility was determined by dye adsorption of Direct Orange (external specific surface) and Direct Blue (internal specific surface). Enzymatic hydrolysis was used to verify the effects of pretreatments and cellulose accessibility on the glucose yield. Delignification by sodium chlorite (oxidative) resulted in lignin removal, with almost complete removal from leaf samples. Accessibility determined indicated that pretreatments that are more aggressive improved cellulose accessibility. The less recalcitrant fraction, the internode, showed 1333.3 mg/g of Direct Orange adsorbed and 746.3 mg/g of Direct Blue. Glucose yield during enzymatic hydrolysis improved with higher cellulose accessibility. Lignin and xylan removal (down to 10% and 1%, respectively) resulted in higher glucose yield, with delignified internode samples showing almost complete cellulose conversion. Hemicellulose and lignin removal by the pretreatments directly influenced cellulose accessibility, resulting in better enzymatic hydrolysis across all fractions. This study successfully showed that lignin and hemicellulose removal of 15% and 10%, respectively, resulting in at least 60% of glucose yield, reaching desired accessibility levels based on dye adsorption of 2079.6 mg/g.
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spelling Minimum Lignin and Xylan Removal to Improve Cellulose AccessibilityAccessibilityAlkaline pretreatmentDiluted acid pretreatmentEnzymatic hydrolysisOxidative pretreatmentThe lignocellulosic biomass, such as provided by the sugarcane, is an abundant source of raw materials for energy production. Milling and pretreatments can be employed to alter the structure of the materials, remove lignin, and hemicellulose. This pretreatment effect exposes the cellulose and raises its accessibility, which is one of the most important properties to ensure enzymatic digestibility. However, the biomass generated from the sugarcane has different physicochemical characteristics, giving different responses to the pretreatments. In this context, this study aimed to verify the effects of lignin and hemicellulose removal from the sugarcane biomass (external fraction, node, internode, and leaf) on cellulose accessibility. Each fraction was pretreated with acid (5, 10, and 20% w/w, at 121 °C/30 min), alkali (5, 10, 20, and 30% NaOH w/w) and oxidative (0.5, 1, 2, and 3 h charged with 30% sodium chlorite). Accessibility was determined by dye adsorption of Direct Orange (external specific surface) and Direct Blue (internal specific surface). Enzymatic hydrolysis was used to verify the effects of pretreatments and cellulose accessibility on the glucose yield. Delignification by sodium chlorite (oxidative) resulted in lignin removal, with almost complete removal from leaf samples. Accessibility determined indicated that pretreatments that are more aggressive improved cellulose accessibility. The less recalcitrant fraction, the internode, showed 1333.3 mg/g of Direct Orange adsorbed and 746.3 mg/g of Direct Blue. Glucose yield during enzymatic hydrolysis improved with higher cellulose accessibility. Lignin and xylan removal (down to 10% and 1%, respectively) resulted in higher glucose yield, with delignified internode samples showing almost complete cellulose conversion. Hemicellulose and lignin removal by the pretreatments directly influenced cellulose accessibility, resulting in better enzymatic hydrolysis across all fractions. This study successfully showed that lignin and hemicellulose removal of 15% and 10%, respectively, resulting in at least 60% of glucose yield, reaching desired accessibility levels based on dye adsorption of 2079.6 mg/g.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Bioenergy Research Institute (IPBEN) Universidade Estadual Paulista (UNESP)Biochemistry and Microbiology Department Universidade Estadual Paulista (UNESP)Bioenergy Research Institute (IPBEN) Universidade Estadual Paulista (UNESP)Biochemistry and Microbiology Department Universidade Estadual Paulista (UNESP)CNPq: 401900/2016-9Universidade Estadual Paulista (Unesp)Shimizu, Felipe Lange [UNESP]de Azevedo, Gabriel Oliveira [UNESP]Coelho, Luciana Fontes [UNESP]Pagnocca, Fernando Carlos [UNESP]Brienzo, Michel [UNESP]2020-12-12T02:02:44Z2020-12-12T02:02:44Z2020-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article775-785http://dx.doi.org/10.1007/s12155-020-10120-zBioenergy Research, v. 13, n. 3, p. 775-785, 2020.1939-12421939-1234http://hdl.handle.net/11449/20028910.1007/s12155-020-10120-z2-s2.0-850833731868251885707409794Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBioenergy Researchinfo:eu-repo/semantics/openAccess2022-03-14T22:05:50Zoai:repositorio.unesp.br:11449/200289Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:24:48.563592Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Minimum Lignin and Xylan Removal to Improve Cellulose Accessibility
title Minimum Lignin and Xylan Removal to Improve Cellulose Accessibility
spellingShingle Minimum Lignin and Xylan Removal to Improve Cellulose Accessibility
Shimizu, Felipe Lange [UNESP]
Accessibility
Alkaline pretreatment
Diluted acid pretreatment
Enzymatic hydrolysis
Oxidative pretreatment
title_short Minimum Lignin and Xylan Removal to Improve Cellulose Accessibility
title_full Minimum Lignin and Xylan Removal to Improve Cellulose Accessibility
title_fullStr Minimum Lignin and Xylan Removal to Improve Cellulose Accessibility
title_full_unstemmed Minimum Lignin and Xylan Removal to Improve Cellulose Accessibility
title_sort Minimum Lignin and Xylan Removal to Improve Cellulose Accessibility
author Shimizu, Felipe Lange [UNESP]
author_facet Shimizu, Felipe Lange [UNESP]
de Azevedo, Gabriel Oliveira [UNESP]
Coelho, Luciana Fontes [UNESP]
Pagnocca, Fernando Carlos [UNESP]
Brienzo, Michel [UNESP]
author_role author
author2 de Azevedo, Gabriel Oliveira [UNESP]
Coelho, Luciana Fontes [UNESP]
Pagnocca, Fernando Carlos [UNESP]
Brienzo, Michel [UNESP]
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Shimizu, Felipe Lange [UNESP]
de Azevedo, Gabriel Oliveira [UNESP]
Coelho, Luciana Fontes [UNESP]
Pagnocca, Fernando Carlos [UNESP]
Brienzo, Michel [UNESP]
dc.subject.por.fl_str_mv Accessibility
Alkaline pretreatment
Diluted acid pretreatment
Enzymatic hydrolysis
Oxidative pretreatment
topic Accessibility
Alkaline pretreatment
Diluted acid pretreatment
Enzymatic hydrolysis
Oxidative pretreatment
description The lignocellulosic biomass, such as provided by the sugarcane, is an abundant source of raw materials for energy production. Milling and pretreatments can be employed to alter the structure of the materials, remove lignin, and hemicellulose. This pretreatment effect exposes the cellulose and raises its accessibility, which is one of the most important properties to ensure enzymatic digestibility. However, the biomass generated from the sugarcane has different physicochemical characteristics, giving different responses to the pretreatments. In this context, this study aimed to verify the effects of lignin and hemicellulose removal from the sugarcane biomass (external fraction, node, internode, and leaf) on cellulose accessibility. Each fraction was pretreated with acid (5, 10, and 20% w/w, at 121 °C/30 min), alkali (5, 10, 20, and 30% NaOH w/w) and oxidative (0.5, 1, 2, and 3 h charged with 30% sodium chlorite). Accessibility was determined by dye adsorption of Direct Orange (external specific surface) and Direct Blue (internal specific surface). Enzymatic hydrolysis was used to verify the effects of pretreatments and cellulose accessibility on the glucose yield. Delignification by sodium chlorite (oxidative) resulted in lignin removal, with almost complete removal from leaf samples. Accessibility determined indicated that pretreatments that are more aggressive improved cellulose accessibility. The less recalcitrant fraction, the internode, showed 1333.3 mg/g of Direct Orange adsorbed and 746.3 mg/g of Direct Blue. Glucose yield during enzymatic hydrolysis improved with higher cellulose accessibility. Lignin and xylan removal (down to 10% and 1%, respectively) resulted in higher glucose yield, with delignified internode samples showing almost complete cellulose conversion. Hemicellulose and lignin removal by the pretreatments directly influenced cellulose accessibility, resulting in better enzymatic hydrolysis across all fractions. This study successfully showed that lignin and hemicellulose removal of 15% and 10%, respectively, resulting in at least 60% of glucose yield, reaching desired accessibility levels based on dye adsorption of 2079.6 mg/g.
publishDate 2020
dc.date.none.fl_str_mv 2020-12-12T02:02:44Z
2020-12-12T02:02:44Z
2020-09-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1007/s12155-020-10120-z
Bioenergy Research, v. 13, n. 3, p. 775-785, 2020.
1939-1242
1939-1234
http://hdl.handle.net/11449/200289
10.1007/s12155-020-10120-z
2-s2.0-85083373186
8251885707409794
url http://dx.doi.org/10.1007/s12155-020-10120-z
http://hdl.handle.net/11449/200289
identifier_str_mv Bioenergy Research, v. 13, n. 3, p. 775-785, 2020.
1939-1242
1939-1234
10.1007/s12155-020-10120-z
2-s2.0-85083373186
8251885707409794
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Bioenergy Research
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 775-785
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
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