Caracterização e infecção de células tronco mesenquimais e células semelhantes a neurônios, derivadas de cordão umbilical bovino pelo herpesvírus bovino Tipo 5 (BoHV-5)
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://hdl.handle.net/11449/121916 |
Resumo: | Bovine Herpesvirus type 5 (BoHV-5) infection results in non- suppurative meningoencephalitis which underlies significant economic losses in South America. Mesenchymal stem cells (MSCs) are found in fetal tissues, such as the umbilical cord. The goal of this research was to characterize the isolation and viability of bovine umbilical cord MSCs for in vitro differentiation into neuron-like cells (NLC) to be used as a model for experimental infection with BoHV-5. MSCs were isolated from Wharton's jelly and differentiated in vitro into adipogenic, chondrogenic, and osteogenic cells as well as NLC. The cells lines were characterized molecularly and immunophenotypically, using PCR, immunocytochemistry, and flow cytometry. The set of primers and panel of primary antibody used in the experiments confirmed the isolation of mesenchymal stem cells and their differentiation into neuronal-like cells. After adaptation and differentiation, the cells were infected with BoHV-5, exhibiting typical cytopathic effect and increased viral replication rate 72 h post-infection. Viral particles were identified in the NLC and in the extracellular environment. by in situ hybridization These results corroborate the use of MSCs as a source of NLC which can be used to study the pathogenesis of the experimental in vitro infection wtih BoHV-5 |
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Caracterização e infecção de células tronco mesenquimais e células semelhantes a neurônios, derivadas de cordão umbilical bovino pelo herpesvírus bovino Tipo 5 (BoHV-5)Geleia de WhartonNeuroniosVirus do herpes em animaisBovinoCelulas - Cultura e meios de culturaBovino - InfecçõesBoHV-5Bovine Herpesvirus type 5 (BoHV-5) infection results in non- suppurative meningoencephalitis which underlies significant economic losses in South America. Mesenchymal stem cells (MSCs) are found in fetal tissues, such as the umbilical cord. The goal of this research was to characterize the isolation and viability of bovine umbilical cord MSCs for in vitro differentiation into neuron-like cells (NLC) to be used as a model for experimental infection with BoHV-5. MSCs were isolated from Wharton's jelly and differentiated in vitro into adipogenic, chondrogenic, and osteogenic cells as well as NLC. The cells lines were characterized molecularly and immunophenotypically, using PCR, immunocytochemistry, and flow cytometry. The set of primers and panel of primary antibody used in the experiments confirmed the isolation of mesenchymal stem cells and their differentiation into neuronal-like cells. After adaptation and differentiation, the cells were infected with BoHV-5, exhibiting typical cytopathic effect and increased viral replication rate 72 h post-infection. Viral particles were identified in the NLC and in the extracellular environment. by in situ hybridization These results corroborate the use of MSCs as a source of NLC which can be used to study the pathogenesis of the experimental in vitro infection wtih BoHV-5Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)FAPESP: 2010/50626-5Universidade Estadual Paulista (Unesp)Cardoso, Tereza Cristina [UNESP]Luvizotto, Maria Cecília Rui [UNESP]Universidade Estadual Paulista (Unesp)Ferrari, Heitor Flávio [UNESP]2015-04-09T12:28:10Z2015-04-09T12:28:10Z2013-06-27info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesis94 f.application/pdfFERRARI, Heitor Flávio. Caracterização e infecção de células tronco mesenquimais e células semelhantes a neurônios, derivadas de cordão umbilical bovino pelo herpesvírus bovino Tipo 5 (BoHV-5). 2013. 94 f. Tese (doutorado) - Universidade Estadual Paulista Júlio de Mesquita Filho, Faculdade de Medicina Veterinária, 2013.http://hdl.handle.net/11449/121916000814098000814098.pdf33004021075P84584674909952477Alephreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPporinfo:eu-repo/semantics/openAccess2024-01-10T06:28:16Zoai:repositorio.unesp.br:11449/121916Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:38:23.877365Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Caracterização e infecção de células tronco mesenquimais e células semelhantes a neurônios, derivadas de cordão umbilical bovino pelo herpesvírus bovino Tipo 5 (BoHV-5) |
title |
Caracterização e infecção de células tronco mesenquimais e células semelhantes a neurônios, derivadas de cordão umbilical bovino pelo herpesvírus bovino Tipo 5 (BoHV-5) |
spellingShingle |
Caracterização e infecção de células tronco mesenquimais e células semelhantes a neurônios, derivadas de cordão umbilical bovino pelo herpesvírus bovino Tipo 5 (BoHV-5) Ferrari, Heitor Flávio [UNESP] Geleia de Wharton Neuronios Virus do herpes em animais Bovino Celulas - Cultura e meios de cultura Bovino - Infecções BoHV-5 |
title_short |
Caracterização e infecção de células tronco mesenquimais e células semelhantes a neurônios, derivadas de cordão umbilical bovino pelo herpesvírus bovino Tipo 5 (BoHV-5) |
title_full |
Caracterização e infecção de células tronco mesenquimais e células semelhantes a neurônios, derivadas de cordão umbilical bovino pelo herpesvírus bovino Tipo 5 (BoHV-5) |
title_fullStr |
Caracterização e infecção de células tronco mesenquimais e células semelhantes a neurônios, derivadas de cordão umbilical bovino pelo herpesvírus bovino Tipo 5 (BoHV-5) |
title_full_unstemmed |
Caracterização e infecção de células tronco mesenquimais e células semelhantes a neurônios, derivadas de cordão umbilical bovino pelo herpesvírus bovino Tipo 5 (BoHV-5) |
title_sort |
Caracterização e infecção de células tronco mesenquimais e células semelhantes a neurônios, derivadas de cordão umbilical bovino pelo herpesvírus bovino Tipo 5 (BoHV-5) |
author |
Ferrari, Heitor Flávio [UNESP] |
author_facet |
Ferrari, Heitor Flávio [UNESP] |
author_role |
author |
dc.contributor.none.fl_str_mv |
Cardoso, Tereza Cristina [UNESP] Luvizotto, Maria Cecília Rui [UNESP] Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Ferrari, Heitor Flávio [UNESP] |
dc.subject.por.fl_str_mv |
Geleia de Wharton Neuronios Virus do herpes em animais Bovino Celulas - Cultura e meios de cultura Bovino - Infecções BoHV-5 |
topic |
Geleia de Wharton Neuronios Virus do herpes em animais Bovino Celulas - Cultura e meios de cultura Bovino - Infecções BoHV-5 |
description |
Bovine Herpesvirus type 5 (BoHV-5) infection results in non- suppurative meningoencephalitis which underlies significant economic losses in South America. Mesenchymal stem cells (MSCs) are found in fetal tissues, such as the umbilical cord. The goal of this research was to characterize the isolation and viability of bovine umbilical cord MSCs for in vitro differentiation into neuron-like cells (NLC) to be used as a model for experimental infection with BoHV-5. MSCs were isolated from Wharton's jelly and differentiated in vitro into adipogenic, chondrogenic, and osteogenic cells as well as NLC. The cells lines were characterized molecularly and immunophenotypically, using PCR, immunocytochemistry, and flow cytometry. The set of primers and panel of primary antibody used in the experiments confirmed the isolation of mesenchymal stem cells and their differentiation into neuronal-like cells. After adaptation and differentiation, the cells were infected with BoHV-5, exhibiting typical cytopathic effect and increased viral replication rate 72 h post-infection. Viral particles were identified in the NLC and in the extracellular environment. by in situ hybridization These results corroborate the use of MSCs as a source of NLC which can be used to study the pathogenesis of the experimental in vitro infection wtih BoHV-5 |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-06-27 2015-04-09T12:28:10Z 2015-04-09T12:28:10Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
FERRARI, Heitor Flávio. Caracterização e infecção de células tronco mesenquimais e células semelhantes a neurônios, derivadas de cordão umbilical bovino pelo herpesvírus bovino Tipo 5 (BoHV-5). 2013. 94 f. Tese (doutorado) - Universidade Estadual Paulista Júlio de Mesquita Filho, Faculdade de Medicina Veterinária, 2013. http://hdl.handle.net/11449/121916 000814098 000814098.pdf 33004021075P8 4584674909952477 |
identifier_str_mv |
FERRARI, Heitor Flávio. Caracterização e infecção de células tronco mesenquimais e células semelhantes a neurônios, derivadas de cordão umbilical bovino pelo herpesvírus bovino Tipo 5 (BoHV-5). 2013. 94 f. Tese (doutorado) - Universidade Estadual Paulista Júlio de Mesquita Filho, Faculdade de Medicina Veterinária, 2013. 000814098 000814098.pdf 33004021075P8 4584674909952477 |
url |
http://hdl.handle.net/11449/121916 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
94 f. application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
publisher.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.source.none.fl_str_mv |
Aleph reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129445968478208 |