Toxicity of Diflubenzuron and Temephos on Freshwater Fishes: Ecotoxicological Assays with Oreochromis niloticus and Hyphessobrycon eques
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s11270-019-4128-7 http://hdl.handle.net/11449/188807 |
Resumo: | Diflubenzuron (DFB) is a larvicide widely used to control Aedes aegypti populations as an alternative to organophosphates (OPs), with a specific mechanism of action for insects by inhibiting their chitin synthesis. However, DFB is used extensively in urban and rural environments, having the aquatic environment as the major receptor. Thus, the present study aimed to investigate the toxicity of DFB-based formulation and compare it with the toxicity of the OP temephos (TMP)-based formulation, a larvicide still used to control A. aegypti, on freshwater fishes Oreochromis niloticus and Hyphessobrycon eques. Organisms were submitted to acute (48 h) and prolonged (7 days) exposures, in the presence and absence of organic sediment, seeking interactions between chemical and sediment. Histopathological analyses were performed on O. niloticus gills and liver. According to 48-h median lethal concentration (LC50), DFB- and TMP-based formulations were classified as harmful and toxic to fish, respectively, following the Globally Harmonized System of Classification (GHS). After prolonged exposure to sublethal concentrations, DFB-based formulation decreased H. eques body weight at concentrations 272-fold lower than its LC50. Ultrastructural responses of O. niloticus indicated edemas and aneurisms on gills, and hepatocyte hypertrophy and vascular congestion of the liver. TMP-based formulation also induced pyknotic nuclei, which may lead to irreversible necrosis. The addition of organic sediment did not alter the larvicide toxicity, suggesting that larvicides remained available to the organisms. Altogether, these results suggest that as an insect-specific pesticide, DFB still induces mortality and tissue damage in fishes; thus, both larvicides pose risks to fishes. |
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Toxicity of Diflubenzuron and Temephos on Freshwater Fishes: Ecotoxicological Assays with Oreochromis niloticus and Hyphessobrycon equesAedes aegyptiLarvicidesMortalityTissue damageDiflubenzuron (DFB) is a larvicide widely used to control Aedes aegypti populations as an alternative to organophosphates (OPs), with a specific mechanism of action for insects by inhibiting their chitin synthesis. However, DFB is used extensively in urban and rural environments, having the aquatic environment as the major receptor. Thus, the present study aimed to investigate the toxicity of DFB-based formulation and compare it with the toxicity of the OP temephos (TMP)-based formulation, a larvicide still used to control A. aegypti, on freshwater fishes Oreochromis niloticus and Hyphessobrycon eques. Organisms were submitted to acute (48 h) and prolonged (7 days) exposures, in the presence and absence of organic sediment, seeking interactions between chemical and sediment. Histopathological analyses were performed on O. niloticus gills and liver. According to 48-h median lethal concentration (LC50), DFB- and TMP-based formulations were classified as harmful and toxic to fish, respectively, following the Globally Harmonized System of Classification (GHS). After prolonged exposure to sublethal concentrations, DFB-based formulation decreased H. eques body weight at concentrations 272-fold lower than its LC50. Ultrastructural responses of O. niloticus indicated edemas and aneurisms on gills, and hepatocyte hypertrophy and vascular congestion of the liver. TMP-based formulation also induced pyknotic nuclei, which may lead to irreversible necrosis. The addition of organic sediment did not alter the larvicide toxicity, suggesting that larvicides remained available to the organisms. Altogether, these results suggest that as an insect-specific pesticide, DFB still induces mortality and tissue damage in fishes; thus, both larvicides pose risks to fishes.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)School of Pharmaceutical Sciences of Ribeirão Preto (FCFRP) University of São Paulo (USP)School of Agricultural and Veterinary Sciences (FCAV) Aquaculture Centre of São Paulo State University (CAUNESP) São Paulo State University (UNESP)University Center of Educational Foundation of Barretos (UNIFEB)School of Agricultural and Veterinary Sciences (FCAV) Aquaculture Centre of São Paulo State University (CAUNESP) São Paulo State University (UNESP)CNPq: 152513/2010-8Universidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)University Center of Educational Foundation of Barretos (UNIFEB)Abe, Flavia R.Machado, Angela A. [UNESP]Coleone, Ana C. [UNESP]da Cruz, ClaudineiMachado-Neto, Joaquim G. [UNESP]2019-10-06T16:19:51Z2019-10-06T16:19:51Z2019-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1007/s11270-019-4128-7Water, Air, and Soil Pollution, v. 230, n. 3, 2019.1573-29320049-6979http://hdl.handle.net/11449/18880710.1007/s11270-019-4128-72-s2.0-85062508756Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengWater, Air, and Soil Pollutioninfo:eu-repo/semantics/openAccess2024-04-09T15:29:58Zoai:repositorio.unesp.br:11449/188807Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:20:02.778418Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Toxicity of Diflubenzuron and Temephos on Freshwater Fishes: Ecotoxicological Assays with Oreochromis niloticus and Hyphessobrycon eques |
title |
Toxicity of Diflubenzuron and Temephos on Freshwater Fishes: Ecotoxicological Assays with Oreochromis niloticus and Hyphessobrycon eques |
spellingShingle |
Toxicity of Diflubenzuron and Temephos on Freshwater Fishes: Ecotoxicological Assays with Oreochromis niloticus and Hyphessobrycon eques Abe, Flavia R. Aedes aegypti Larvicides Mortality Tissue damage |
title_short |
Toxicity of Diflubenzuron and Temephos on Freshwater Fishes: Ecotoxicological Assays with Oreochromis niloticus and Hyphessobrycon eques |
title_full |
Toxicity of Diflubenzuron and Temephos on Freshwater Fishes: Ecotoxicological Assays with Oreochromis niloticus and Hyphessobrycon eques |
title_fullStr |
Toxicity of Diflubenzuron and Temephos on Freshwater Fishes: Ecotoxicological Assays with Oreochromis niloticus and Hyphessobrycon eques |
title_full_unstemmed |
Toxicity of Diflubenzuron and Temephos on Freshwater Fishes: Ecotoxicological Assays with Oreochromis niloticus and Hyphessobrycon eques |
title_sort |
Toxicity of Diflubenzuron and Temephos on Freshwater Fishes: Ecotoxicological Assays with Oreochromis niloticus and Hyphessobrycon eques |
author |
Abe, Flavia R. |
author_facet |
Abe, Flavia R. Machado, Angela A. [UNESP] Coleone, Ana C. [UNESP] da Cruz, Claudinei Machado-Neto, Joaquim G. [UNESP] |
author_role |
author |
author2 |
Machado, Angela A. [UNESP] Coleone, Ana C. [UNESP] da Cruz, Claudinei Machado-Neto, Joaquim G. [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Universidade Estadual Paulista (Unesp) University Center of Educational Foundation of Barretos (UNIFEB) |
dc.contributor.author.fl_str_mv |
Abe, Flavia R. Machado, Angela A. [UNESP] Coleone, Ana C. [UNESP] da Cruz, Claudinei Machado-Neto, Joaquim G. [UNESP] |
dc.subject.por.fl_str_mv |
Aedes aegypti Larvicides Mortality Tissue damage |
topic |
Aedes aegypti Larvicides Mortality Tissue damage |
description |
Diflubenzuron (DFB) is a larvicide widely used to control Aedes aegypti populations as an alternative to organophosphates (OPs), with a specific mechanism of action for insects by inhibiting their chitin synthesis. However, DFB is used extensively in urban and rural environments, having the aquatic environment as the major receptor. Thus, the present study aimed to investigate the toxicity of DFB-based formulation and compare it with the toxicity of the OP temephos (TMP)-based formulation, a larvicide still used to control A. aegypti, on freshwater fishes Oreochromis niloticus and Hyphessobrycon eques. Organisms were submitted to acute (48 h) and prolonged (7 days) exposures, in the presence and absence of organic sediment, seeking interactions between chemical and sediment. Histopathological analyses were performed on O. niloticus gills and liver. According to 48-h median lethal concentration (LC50), DFB- and TMP-based formulations were classified as harmful and toxic to fish, respectively, following the Globally Harmonized System of Classification (GHS). After prolonged exposure to sublethal concentrations, DFB-based formulation decreased H. eques body weight at concentrations 272-fold lower than its LC50. Ultrastructural responses of O. niloticus indicated edemas and aneurisms on gills, and hepatocyte hypertrophy and vascular congestion of the liver. TMP-based formulation also induced pyknotic nuclei, which may lead to irreversible necrosis. The addition of organic sediment did not alter the larvicide toxicity, suggesting that larvicides remained available to the organisms. Altogether, these results suggest that as an insect-specific pesticide, DFB still induces mortality and tissue damage in fishes; thus, both larvicides pose risks to fishes. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-06T16:19:51Z 2019-10-06T16:19:51Z 2019-03-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s11270-019-4128-7 Water, Air, and Soil Pollution, v. 230, n. 3, 2019. 1573-2932 0049-6979 http://hdl.handle.net/11449/188807 10.1007/s11270-019-4128-7 2-s2.0-85062508756 |
url |
http://dx.doi.org/10.1007/s11270-019-4128-7 http://hdl.handle.net/11449/188807 |
identifier_str_mv |
Water, Air, and Soil Pollution, v. 230, n. 3, 2019. 1573-2932 0049-6979 10.1007/s11270-019-4128-7 2-s2.0-85062508756 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Water, Air, and Soil Pollution |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808128921039798272 |