Hyptis suaveolens (L.) Poit protects colon from TNBS-induced inflammation via immunomodulatory, antioxidant and anti-proliferative mechanisms
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.jep.2020.113153 http://hdl.handle.net/11449/208021 |
Resumo: | Ethnopharmacological relevance: In folk medicine Hyptis suaveolens (Lamiaceae) has been reported to relieve respiratory and gastrointestinal infections, indigestion, cold, pain, fever, cramps, skin diseases, gastric ulcer and inflammatory disorders. This study investigated the effects and the mechanisms of action of Hyptis suaveolens (L.) Poit (Lamiaceae) ethanol extract (Hs-EtOH) and hexane phase (Hs-HexF) against intestinal inflammation. Material and methods: Acute and relapse TNBS-induced ulcerative colitis protocols were used to evaluate intestinal anti-inflammatory activity. Damage evaluations, biochemical, histological and immunostaining parameters were determined. Results: Both extracts decreased macroscopic colonic inflammation and the area of lesion induced by TNBS. Nevertheless, only Hs-HexF was able to reduce colonic wall thickness, edema and diffuse inflammatory cell infiltration and to prevent GSH depletion in the acute model of ulcerative colitis. In the chronic phase with relapse of colonic ulceration, yet again only Hs-HexF significantly attenuated inflammatory parameters and presented a decrease in nitrite/nitrate, MDA, MPO, IL-1-β and TNF-α and increased levels of SOD, CAT, GSH and IL-10. Hs-HexF also significantly reduced positive cells immunostained for PCNA. Conclusion: The data indicate intestinal anti-inflammatory activity for H. suaveolens, due to the participation of the antioxidant system, decreased neutrophil infiltration and cytokine modulation, as well as, owing to regulation of cell proliferation. |
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Hyptis suaveolens (L.) Poit protects colon from TNBS-induced inflammation via immunomodulatory, antioxidant and anti-proliferative mechanismsHyptis suaveolensIntestinal anti-inflammatory activityMedicinal plantEthnopharmacological relevance: In folk medicine Hyptis suaveolens (Lamiaceae) has been reported to relieve respiratory and gastrointestinal infections, indigestion, cold, pain, fever, cramps, skin diseases, gastric ulcer and inflammatory disorders. This study investigated the effects and the mechanisms of action of Hyptis suaveolens (L.) Poit (Lamiaceae) ethanol extract (Hs-EtOH) and hexane phase (Hs-HexF) against intestinal inflammation. Material and methods: Acute and relapse TNBS-induced ulcerative colitis protocols were used to evaluate intestinal anti-inflammatory activity. Damage evaluations, biochemical, histological and immunostaining parameters were determined. Results: Both extracts decreased macroscopic colonic inflammation and the area of lesion induced by TNBS. Nevertheless, only Hs-HexF was able to reduce colonic wall thickness, edema and diffuse inflammatory cell infiltration and to prevent GSH depletion in the acute model of ulcerative colitis. In the chronic phase with relapse of colonic ulceration, yet again only Hs-HexF significantly attenuated inflammatory parameters and presented a decrease in nitrite/nitrate, MDA, MPO, IL-1-β and TNF-α and increased levels of SOD, CAT, GSH and IL-10. Hs-HexF also significantly reduced positive cells immunostained for PCNA. Conclusion: The data indicate intestinal anti-inflammatory activity for H. suaveolens, due to the participation of the antioxidant system, decreased neutrophil infiltration and cytokine modulation, as well as, owing to regulation of cell proliferation.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Postgraduate Program in Natural and Synthetic Bioactive Products Health Sciences Center Universidade Federal da Paraíba (UFPB)Laboratory of Natural Products Universidade Federal do Ceará (UFC)Department of Biophysics and Pharmacology Universidade Federal do Rio Grande Norte (UFRN)Department of Morphology Histology and Basic Pathology Federal University of Rio Grande do NorteLaboratory of Histology UNIPEX-FMB Universidade de São Paulo (UNESP)Laboratory of Histology UNIPEX-FMB Universidade de São Paulo (UNESP)CNPq: 14/2014Universidade Federal da Paraíba (UFPB)Universidade Federal do Ceará (UFC)Universidade Federal do Rio Grande Norte (UFRN)Federal University of Rio Grande do NorteUniversidade Estadual Paulista (Unesp)Machado, Flávia Danniele FrotaFormiga, Rodrigo de OliveiraLima, Gedson Rodrigues de Moraisde Jesus, Neyres Zínia TaveiraAlves Júnior, Edvaldo BalbinoMarinho, Alexsandro FernandesTavares, Josean FechineSantos, Flávia AlmeidaViana, Ana Flávia Seraine CustódioAraújo, Aurigena Antunesde Araújo Júnior, Raimundo FernandesPellizzon, Cláudia Helena [UNESP]Batista, Leônia Maria2021-06-25T11:05:01Z2021-06-25T11:05:01Z2021-01-30info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.jep.2020.113153Journal of Ethnopharmacology, v. 265.1872-75730378-8741http://hdl.handle.net/11449/20802110.1016/j.jep.2020.1131532-s2.0-8509206723900193937798010690000-0002-4494-4180Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Ethnopharmacologyinfo:eu-repo/semantics/openAccess2021-10-27T11:20:52Zoai:repositorio.unesp.br:11449/208021Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-27T11:20:52Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Hyptis suaveolens (L.) Poit protects colon from TNBS-induced inflammation via immunomodulatory, antioxidant and anti-proliferative mechanisms |
title |
Hyptis suaveolens (L.) Poit protects colon from TNBS-induced inflammation via immunomodulatory, antioxidant and anti-proliferative mechanisms |
spellingShingle |
Hyptis suaveolens (L.) Poit protects colon from TNBS-induced inflammation via immunomodulatory, antioxidant and anti-proliferative mechanisms Machado, Flávia Danniele Frota Hyptis suaveolens Intestinal anti-inflammatory activity Medicinal plant |
title_short |
Hyptis suaveolens (L.) Poit protects colon from TNBS-induced inflammation via immunomodulatory, antioxidant and anti-proliferative mechanisms |
title_full |
Hyptis suaveolens (L.) Poit protects colon from TNBS-induced inflammation via immunomodulatory, antioxidant and anti-proliferative mechanisms |
title_fullStr |
Hyptis suaveolens (L.) Poit protects colon from TNBS-induced inflammation via immunomodulatory, antioxidant and anti-proliferative mechanisms |
title_full_unstemmed |
Hyptis suaveolens (L.) Poit protects colon from TNBS-induced inflammation via immunomodulatory, antioxidant and anti-proliferative mechanisms |
title_sort |
Hyptis suaveolens (L.) Poit protects colon from TNBS-induced inflammation via immunomodulatory, antioxidant and anti-proliferative mechanisms |
author |
Machado, Flávia Danniele Frota |
author_facet |
Machado, Flávia Danniele Frota Formiga, Rodrigo de Oliveira Lima, Gedson Rodrigues de Morais de Jesus, Neyres Zínia Taveira Alves Júnior, Edvaldo Balbino Marinho, Alexsandro Fernandes Tavares, Josean Fechine Santos, Flávia Almeida Viana, Ana Flávia Seraine Custódio Araújo, Aurigena Antunes de Araújo Júnior, Raimundo Fernandes Pellizzon, Cláudia Helena [UNESP] Batista, Leônia Maria |
author_role |
author |
author2 |
Formiga, Rodrigo de Oliveira Lima, Gedson Rodrigues de Morais de Jesus, Neyres Zínia Taveira Alves Júnior, Edvaldo Balbino Marinho, Alexsandro Fernandes Tavares, Josean Fechine Santos, Flávia Almeida Viana, Ana Flávia Seraine Custódio Araújo, Aurigena Antunes de Araújo Júnior, Raimundo Fernandes Pellizzon, Cláudia Helena [UNESP] Batista, Leônia Maria |
author2_role |
author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal da Paraíba (UFPB) Universidade Federal do Ceará (UFC) Universidade Federal do Rio Grande Norte (UFRN) Federal University of Rio Grande do Norte Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Machado, Flávia Danniele Frota Formiga, Rodrigo de Oliveira Lima, Gedson Rodrigues de Morais de Jesus, Neyres Zínia Taveira Alves Júnior, Edvaldo Balbino Marinho, Alexsandro Fernandes Tavares, Josean Fechine Santos, Flávia Almeida Viana, Ana Flávia Seraine Custódio Araújo, Aurigena Antunes de Araújo Júnior, Raimundo Fernandes Pellizzon, Cláudia Helena [UNESP] Batista, Leônia Maria |
dc.subject.por.fl_str_mv |
Hyptis suaveolens Intestinal anti-inflammatory activity Medicinal plant |
topic |
Hyptis suaveolens Intestinal anti-inflammatory activity Medicinal plant |
description |
Ethnopharmacological relevance: In folk medicine Hyptis suaveolens (Lamiaceae) has been reported to relieve respiratory and gastrointestinal infections, indigestion, cold, pain, fever, cramps, skin diseases, gastric ulcer and inflammatory disorders. This study investigated the effects and the mechanisms of action of Hyptis suaveolens (L.) Poit (Lamiaceae) ethanol extract (Hs-EtOH) and hexane phase (Hs-HexF) against intestinal inflammation. Material and methods: Acute and relapse TNBS-induced ulcerative colitis protocols were used to evaluate intestinal anti-inflammatory activity. Damage evaluations, biochemical, histological and immunostaining parameters were determined. Results: Both extracts decreased macroscopic colonic inflammation and the area of lesion induced by TNBS. Nevertheless, only Hs-HexF was able to reduce colonic wall thickness, edema and diffuse inflammatory cell infiltration and to prevent GSH depletion in the acute model of ulcerative colitis. In the chronic phase with relapse of colonic ulceration, yet again only Hs-HexF significantly attenuated inflammatory parameters and presented a decrease in nitrite/nitrate, MDA, MPO, IL-1-β and TNF-α and increased levels of SOD, CAT, GSH and IL-10. Hs-HexF also significantly reduced positive cells immunostained for PCNA. Conclusion: The data indicate intestinal anti-inflammatory activity for H. suaveolens, due to the participation of the antioxidant system, decreased neutrophil infiltration and cytokine modulation, as well as, owing to regulation of cell proliferation. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-25T11:05:01Z 2021-06-25T11:05:01Z 2021-01-30 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.jep.2020.113153 Journal of Ethnopharmacology, v. 265. 1872-7573 0378-8741 http://hdl.handle.net/11449/208021 10.1016/j.jep.2020.113153 2-s2.0-85092067239 0019393779801069 0000-0002-4494-4180 |
url |
http://dx.doi.org/10.1016/j.jep.2020.113153 http://hdl.handle.net/11449/208021 |
identifier_str_mv |
Journal of Ethnopharmacology, v. 265. 1872-7573 0378-8741 10.1016/j.jep.2020.113153 2-s2.0-85092067239 0019393779801069 0000-0002-4494-4180 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Ethnopharmacology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1803047319404281856 |