Terpinen-4-ol and carvacrol affect multi-species biofilm composition

• Autor(a) principal: Maquera Huacho, Patricia Milagros [UNESP]
• Data de Publicação: 2019
• Outros Autores: Rodriguez Herrero, Esteban, Verspecht, Tim, Pauwels, Martine, Marcantonio, Elcio [UNESP], Palomari Spolidorio, Denise Madalena [UNESP], Teughels, Wim
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://dx.doi.org/10.1080/08927014.2019.1630386; http://hdl.handle.net/11449/190473
• topic: carvacrol; multi-species biofilm; peri-implantitis; periodontal disease; Terpinen-4-ol

The aim of this study was to investigate the cytotoxic activity and inhibitory effect of terpinen-4-ol (T4ol) and carvacrol against single- and multi-species biofilms. The toxicity of each compound was tested on oral keratinocytes and evaluated by XTT assay. Inhibition and eradication of single-species biofilms were analyzed by crystal violet assay and the effect on multi-species biofilm composition was evaluated by qPCR. T4ol and carvacrol did not affect the epithelial cell viability, in contrast to chlorhexidine, which showed a high cytotoxic effect. Inhibition and eradication of single-species biofilms treated with T4ol and carvacrol were observed. The same inhibitory effect was observed for multi-species biofilms, especially on periodontal pathogens. In conclusion, specific concentrations of T4ol and carvacrol without toxicity towards the epithelial cells reduced the numbers of periodontal pathogens in single- and multi-species biofilms.