Prevalence of nasal carriers of methicillin-resistant Staphylococcus aureus in primary health care units in Brazil
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S1678-9946202163014 http://hdl.handle.net/11449/210106 |
Resumo: | Nasal carriage of Staphylococcus aureus by healthcare workers is of great clinical importance as it facilitates the contamination of medical devices and cross-transmission. However, studies regarding the epidemiology and dissemination of S. aureus and Methicillin-resistant S. aureus (MRSA) within the Primary Health Care in Brazil are scarce. The current study aimed to detect and characterize S. aureus and MRSA strains from the nasal cavities of 63 healthcare working in primary health care units in order to determine the prevalence of S. aureus and MRSA, biofilm formation and resistance profile of these isolates. PCR reactions were performed for detecting mecA, icaA and ical) genes. The phenotypic antimicrobial susceptibility was assessed by the disk diffusion method and biofilm formation by the Congo Red Agar (CRA) method. The MRSA isolates were typed for the Staphylococcal Cassette Chromosome mec (SCCmec). The prevalence of nasal carriage of S. aureus was 74.6%. of which 72.3% were MRSA carrying SCCmec type I (24.4%), III (34.1%), IV (36.6%). Two (4.9%) isolates presented a non-typeable cassette by the performed technique. The antimicrobial susceptibility evaluation evidenced penicillin resistance in 66.1% of S. aureus, erythromycin resistance in 49.2%. while 37.3% were resistant to oxacillin, 28.8% to cefoxitin, 5.1% to levofloxacin and 5.1% to clindamycin. All isolates were biofilm producers and 96.6% of the strains contained the ica biofilm-forming genes (icaA and/or ical)). We have demonstrated a high prevalence of S. aureus and MRSA carriage among health care working in Primary health Care units, the presence of SCCmec types I, III and IV, in addition to their high ability to form biofilm, factors that possibly contribute to the dissemination and persistence of these pathogens within the primary care services. These observations highlight the importance of broadening the perspective of Health Care-Associated Infections prevention, including all health care levels, which are currently little explored. In addition, the dynamics and resistance mechanisms of S. aureus transmission still need to be further clarified to enable the implementation of more effective prevention measures. |
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Prevalence of nasal carriers of methicillin-resistant Staphylococcus aureus in primary health care units in BrazilHealthcare workersFamily Health StrategyCross-transmissionMethicillin-resistantBiofilmOperon icaSCCmecNasal carriage of Staphylococcus aureus by healthcare workers is of great clinical importance as it facilitates the contamination of medical devices and cross-transmission. However, studies regarding the epidemiology and dissemination of S. aureus and Methicillin-resistant S. aureus (MRSA) within the Primary Health Care in Brazil are scarce. The current study aimed to detect and characterize S. aureus and MRSA strains from the nasal cavities of 63 healthcare working in primary health care units in order to determine the prevalence of S. aureus and MRSA, biofilm formation and resistance profile of these isolates. PCR reactions were performed for detecting mecA, icaA and ical) genes. The phenotypic antimicrobial susceptibility was assessed by the disk diffusion method and biofilm formation by the Congo Red Agar (CRA) method. The MRSA isolates were typed for the Staphylococcal Cassette Chromosome mec (SCCmec). The prevalence of nasal carriage of S. aureus was 74.6%. of which 72.3% were MRSA carrying SCCmec type I (24.4%), III (34.1%), IV (36.6%). Two (4.9%) isolates presented a non-typeable cassette by the performed technique. The antimicrobial susceptibility evaluation evidenced penicillin resistance in 66.1% of S. aureus, erythromycin resistance in 49.2%. while 37.3% were resistant to oxacillin, 28.8% to cefoxitin, 5.1% to levofloxacin and 5.1% to clindamycin. All isolates were biofilm producers and 96.6% of the strains contained the ica biofilm-forming genes (icaA and/or ical)). We have demonstrated a high prevalence of S. aureus and MRSA carriage among health care working in Primary health Care units, the presence of SCCmec types I, III and IV, in addition to their high ability to form biofilm, factors that possibly contribute to the dissemination and persistence of these pathogens within the primary care services. These observations highlight the importance of broadening the perspective of Health Care-Associated Infections prevention, including all health care levels, which are currently little explored. In addition, the dynamics and resistance mechanisms of S. aureus transmission still need to be further clarified to enable the implementation of more effective prevention measures.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Univ Oeste Paulista, Rua Jose Bongiovani 700, BR-19050920 Presidente Prudente, SP, BrazilUniv Estadual Paulista, Dept Ciencias Quim & Biol, Botucatu, SP, BrazilInst Lauro Souza Lima, Dept Patol, Bauru, SP, BrazilUniv Estadual Paulista, Dept Ciencias Quim & Biol, Botucatu, SP, BrazilFAPESP: 2017/01104-5Inst Medicina Tropical Sao PauloUniv Oeste PaulistaUniversidade Estadual Paulista (Unesp)Inst Lauro Souza LimaRodrigues dos Santos Goes, Isabella CarolinaRomero, Leticia Calixto [UNESP]Turra, Ana JuliaGotardi, Maria AngelicaSilva de Oliveira Rodrigues, Talyssa FernandesSantos, Lalessa de Oliveiradas Dores, Jessica CristinaNascimento, Michel Ulloffo doCavalleri, Andressa CortesPinheiro-Hubinger, Luiza [UNESP]Winkelstroter Eller, Lizziane KretliPereira, Valeria Cataneli2021-06-25T12:39:56Z2021-06-25T12:39:56Z2021-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article9application/pdfhttp://dx.doi.org/10.1590/S1678-9946202163014Revista Do Instituto De Medicina Tropical De Sao Paulo. Sao Paulo: Inst Medicina Tropical Sao Paulo, v. 63, 9 p., 2021.0036-4665http://hdl.handle.net/11449/21010610.1590/S1678-9946202163014S0036-46652021000100208WOS:000626299300002S0036-46652021000100208.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengRevista Do Instituto De Medicina Tropical De Sao Pauloinfo:eu-repo/semantics/openAccess2023-12-28T06:21:07Zoai:repositorio.unesp.br:11449/210106Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:32:30.220244Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Prevalence of nasal carriers of methicillin-resistant Staphylococcus aureus in primary health care units in Brazil |
title |
Prevalence of nasal carriers of methicillin-resistant Staphylococcus aureus in primary health care units in Brazil |
spellingShingle |
Prevalence of nasal carriers of methicillin-resistant Staphylococcus aureus in primary health care units in Brazil Rodrigues dos Santos Goes, Isabella Carolina Healthcare workers Family Health Strategy Cross-transmission Methicillin-resistant Biofilm Operon ica SCCmec |
title_short |
Prevalence of nasal carriers of methicillin-resistant Staphylococcus aureus in primary health care units in Brazil |
title_full |
Prevalence of nasal carriers of methicillin-resistant Staphylococcus aureus in primary health care units in Brazil |
title_fullStr |
Prevalence of nasal carriers of methicillin-resistant Staphylococcus aureus in primary health care units in Brazil |
title_full_unstemmed |
Prevalence of nasal carriers of methicillin-resistant Staphylococcus aureus in primary health care units in Brazil |
title_sort |
Prevalence of nasal carriers of methicillin-resistant Staphylococcus aureus in primary health care units in Brazil |
author |
Rodrigues dos Santos Goes, Isabella Carolina |
author_facet |
Rodrigues dos Santos Goes, Isabella Carolina Romero, Leticia Calixto [UNESP] Turra, Ana Julia Gotardi, Maria Angelica Silva de Oliveira Rodrigues, Talyssa Fernandes Santos, Lalessa de Oliveira das Dores, Jessica Cristina Nascimento, Michel Ulloffo do Cavalleri, Andressa Cortes Pinheiro-Hubinger, Luiza [UNESP] Winkelstroter Eller, Lizziane Kretli Pereira, Valeria Cataneli |
author_role |
author |
author2 |
Romero, Leticia Calixto [UNESP] Turra, Ana Julia Gotardi, Maria Angelica Silva de Oliveira Rodrigues, Talyssa Fernandes Santos, Lalessa de Oliveira das Dores, Jessica Cristina Nascimento, Michel Ulloffo do Cavalleri, Andressa Cortes Pinheiro-Hubinger, Luiza [UNESP] Winkelstroter Eller, Lizziane Kretli Pereira, Valeria Cataneli |
author2_role |
author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Univ Oeste Paulista Universidade Estadual Paulista (Unesp) Inst Lauro Souza Lima |
dc.contributor.author.fl_str_mv |
Rodrigues dos Santos Goes, Isabella Carolina Romero, Leticia Calixto [UNESP] Turra, Ana Julia Gotardi, Maria Angelica Silva de Oliveira Rodrigues, Talyssa Fernandes Santos, Lalessa de Oliveira das Dores, Jessica Cristina Nascimento, Michel Ulloffo do Cavalleri, Andressa Cortes Pinheiro-Hubinger, Luiza [UNESP] Winkelstroter Eller, Lizziane Kretli Pereira, Valeria Cataneli |
dc.subject.por.fl_str_mv |
Healthcare workers Family Health Strategy Cross-transmission Methicillin-resistant Biofilm Operon ica SCCmec |
topic |
Healthcare workers Family Health Strategy Cross-transmission Methicillin-resistant Biofilm Operon ica SCCmec |
description |
Nasal carriage of Staphylococcus aureus by healthcare workers is of great clinical importance as it facilitates the contamination of medical devices and cross-transmission. However, studies regarding the epidemiology and dissemination of S. aureus and Methicillin-resistant S. aureus (MRSA) within the Primary Health Care in Brazil are scarce. The current study aimed to detect and characterize S. aureus and MRSA strains from the nasal cavities of 63 healthcare working in primary health care units in order to determine the prevalence of S. aureus and MRSA, biofilm formation and resistance profile of these isolates. PCR reactions were performed for detecting mecA, icaA and ical) genes. The phenotypic antimicrobial susceptibility was assessed by the disk diffusion method and biofilm formation by the Congo Red Agar (CRA) method. The MRSA isolates were typed for the Staphylococcal Cassette Chromosome mec (SCCmec). The prevalence of nasal carriage of S. aureus was 74.6%. of which 72.3% were MRSA carrying SCCmec type I (24.4%), III (34.1%), IV (36.6%). Two (4.9%) isolates presented a non-typeable cassette by the performed technique. The antimicrobial susceptibility evaluation evidenced penicillin resistance in 66.1% of S. aureus, erythromycin resistance in 49.2%. while 37.3% were resistant to oxacillin, 28.8% to cefoxitin, 5.1% to levofloxacin and 5.1% to clindamycin. All isolates were biofilm producers and 96.6% of the strains contained the ica biofilm-forming genes (icaA and/or ical)). We have demonstrated a high prevalence of S. aureus and MRSA carriage among health care working in Primary health Care units, the presence of SCCmec types I, III and IV, in addition to their high ability to form biofilm, factors that possibly contribute to the dissemination and persistence of these pathogens within the primary care services. These observations highlight the importance of broadening the perspective of Health Care-Associated Infections prevention, including all health care levels, which are currently little explored. In addition, the dynamics and resistance mechanisms of S. aureus transmission still need to be further clarified to enable the implementation of more effective prevention measures. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-25T12:39:56Z 2021-06-25T12:39:56Z 2021-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S1678-9946202163014 Revista Do Instituto De Medicina Tropical De Sao Paulo. Sao Paulo: Inst Medicina Tropical Sao Paulo, v. 63, 9 p., 2021. 0036-4665 http://hdl.handle.net/11449/210106 10.1590/S1678-9946202163014 S0036-46652021000100208 WOS:000626299300002 S0036-46652021000100208.pdf |
url |
http://dx.doi.org/10.1590/S1678-9946202163014 http://hdl.handle.net/11449/210106 |
identifier_str_mv |
Revista Do Instituto De Medicina Tropical De Sao Paulo. Sao Paulo: Inst Medicina Tropical Sao Paulo, v. 63, 9 p., 2021. 0036-4665 10.1590/S1678-9946202163014 S0036-46652021000100208 WOS:000626299300002 S0036-46652021000100208.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
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Revista Do Instituto De Medicina Tropical De Sao Paulo |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
9 application/pdf |
dc.publisher.none.fl_str_mv |
Inst Medicina Tropical Sao Paulo |
publisher.none.fl_str_mv |
Inst Medicina Tropical Sao Paulo |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
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UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808129332527235072 |