Influence of a probiotic soy product on fecal microbiota and its association with cardiovascular risk factors in a animal model
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://link.springer.com/article/10.1186%2F1476-511X-10-126 http://hdl.handle.net/11449/133699 |
Resumo: | Background: Previous work showed that daily ingestion of an aqueous soy extract fermented with Enterococcus faecium CRL 183 and Lactobacillus helveticus 416, supplemented or not with isoflavones, reduced the total cholesterol and non-HDL-cholesterol levels, increased the high-density lipoprotein (HDL) concentration and inhibited the raising of autoantibody against oxidized low-density lipoprotein (ox-LDL Ab) and the development of atherosclerotic lesions. Objective: The aim of this study was to characterize the fecal microbiota in order to investigate the possible correlation between fecal microbiota, serum lipid parameters and atherosclerotic lesion development in rabbits with induced hypercholesterolemia, that ingested the aqueous soy extract fermented with Enterococcus faecium CRL 183 and Lactobacillus helveticus 416. Methods: The rabbits were randomly allocated to five experimental groups (n = 6): control (C), hypercholesterolemic (H), hypercholesterolemic plus unfermented soy product (HUF), hypercholesterolemic plus fermented soy product (HF) and hypercholesterolemic plus isoflavone-supplemented fermented soy product (HIF). Lipid parameters and microbiota composition were analyzed on days 0 and 60 of the treatment and the atherosclerotic lesions were quantified at the end of the experiment. The fecal microbiota was characterized by enumerating the Lactobacillus spp., Bifidobacterium spp., Enterococcus spp., Enterobacteria and Clostridium spp. populations. Results: After 60 days of the experiment, intake of the probiotic soy product was correlated with significant increases (P < 0.05) on Lactobacillus spp., Bifidobacterium spp. and Enterococcus spp. and a decrease in the Enterobacteria population. A strong correlation was observed between microbiota composition and lipid profile. Populations of Enterococcus spp., Lactobacillus spp. and Bifidobacterium spp. were negatively correlated with total cholesterol, non-HDL-cholesterol, autoantibodies against oxidized LDL (oxLDL Ab) and lesion size. HDL-C levels were positively correlated with Lactobacillus spp., Bifidobacterium spp., and Enterococcus spp. populations. Conclusion: In conclusion, daily ingestion of the probiotic soy product, supplemented or not with isoflavones, may contribute to a beneficial balance of the fecal microbiota and this modulation is associated with an improved cholesterol profile and inhibition of atherosclerotic lesion development. |
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Influence of a probiotic soy product on fecal microbiota and its association with cardiovascular risk factors in a animal modelProbioticsEnterococcus faecium CRL 183Fecal microbiotaLipid parametersBackground: Previous work showed that daily ingestion of an aqueous soy extract fermented with Enterococcus faecium CRL 183 and Lactobacillus helveticus 416, supplemented or not with isoflavones, reduced the total cholesterol and non-HDL-cholesterol levels, increased the high-density lipoprotein (HDL) concentration and inhibited the raising of autoantibody against oxidized low-density lipoprotein (ox-LDL Ab) and the development of atherosclerotic lesions. Objective: The aim of this study was to characterize the fecal microbiota in order to investigate the possible correlation between fecal microbiota, serum lipid parameters and atherosclerotic lesion development in rabbits with induced hypercholesterolemia, that ingested the aqueous soy extract fermented with Enterococcus faecium CRL 183 and Lactobacillus helveticus 416. Methods: The rabbits were randomly allocated to five experimental groups (n = 6): control (C), hypercholesterolemic (H), hypercholesterolemic plus unfermented soy product (HUF), hypercholesterolemic plus fermented soy product (HF) and hypercholesterolemic plus isoflavone-supplemented fermented soy product (HIF). Lipid parameters and microbiota composition were analyzed on days 0 and 60 of the treatment and the atherosclerotic lesions were quantified at the end of the experiment. The fecal microbiota was characterized by enumerating the Lactobacillus spp., Bifidobacterium spp., Enterococcus spp., Enterobacteria and Clostridium spp. populations. Results: After 60 days of the experiment, intake of the probiotic soy product was correlated with significant increases (P < 0.05) on Lactobacillus spp., Bifidobacterium spp. and Enterococcus spp. and a decrease in the Enterobacteria population. A strong correlation was observed between microbiota composition and lipid profile. Populations of Enterococcus spp., Lactobacillus spp. and Bifidobacterium spp. were negatively correlated with total cholesterol, non-HDL-cholesterol, autoantibodies against oxidized LDL (oxLDL Ab) and lesion size. HDL-C levels were positively correlated with Lactobacillus spp., Bifidobacterium spp., and Enterococcus spp. populations. Conclusion: In conclusion, daily ingestion of the probiotic soy product, supplemented or not with isoflavones, may contribute to a beneficial balance of the fecal microbiota and this modulation is associated with an improved cholesterol profile and inhibition of atherosclerotic lesion development.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Food and Nutrition, School of Pharmaceutical Sciences, Sao Paulo State University (UNESP), Araraquara, SP, BrazilDepartment of Clinical and Toxicological Analyses, School of Pharmaceutical Sciences, University of Sao Paulo (USP), Sao Paulo, BrazilDepartment of Clinical Analysis, School of Pharmaceutical Sciences, Sao Paulo State University (UNESP) Araraquara, SP, BrazilDepartment of Food and Nutrition, School of Pharmaceutical Sciences, Sao Paulo State University (UNESP), Araraquara, SP, BrazilDepartment of Clinical and Toxicological Analyses, School of Pharmaceutical Sciences, University of Sao Paulo (USP), Sao Paulo, BrazilDepartment of Clinical Analysis, School of Pharmaceutical Sciences, Sao Paulo State University (UNESP) Araraquara, SP, Brazil.Universidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Cavallini, Daniela Cardoso Umbelino [UNESP]Suzuki, Juliana Y. [UNESP]Abdalla, Dulcinéia S PVendramini, Regina C. [UNESP]Pauly-silveira, Nadiége D [UNESP]Roselino, Mariana N [UNESP]Pinto, Roseli Ap. [UNESP]Rossi, Elizeu Antonio [UNESP]2016-01-28T16:56:13Z2016-01-28T16:56:13Z2011info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1-9application/pdfhttp://link.springer.com/article/10.1186%2F1476-511X-10-126Lipids in health and disease, v. 10, n. 126, p. 1-9, 2011.1476-511Xhttp://hdl.handle.net/11449/13369910.1186/1476-511X-10-126ISSN1476-511X-2011-10-126-1-9.pdf463159824137286132428585357637939905593337419625854642079994778376055682388874691194360710417891Currículo Lattesreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengLipids in health and disease2.663info:eu-repo/semantics/openAccess2024-06-24T14:51:41Zoai:repositorio.unesp.br:11449/133699Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:25:25.428468Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Influence of a probiotic soy product on fecal microbiota and its association with cardiovascular risk factors in a animal model |
title |
Influence of a probiotic soy product on fecal microbiota and its association with cardiovascular risk factors in a animal model |
spellingShingle |
Influence of a probiotic soy product on fecal microbiota and its association with cardiovascular risk factors in a animal model Cavallini, Daniela Cardoso Umbelino [UNESP] Probiotics Enterococcus faecium CRL 183 Fecal microbiota Lipid parameters |
title_short |
Influence of a probiotic soy product on fecal microbiota and its association with cardiovascular risk factors in a animal model |
title_full |
Influence of a probiotic soy product on fecal microbiota and its association with cardiovascular risk factors in a animal model |
title_fullStr |
Influence of a probiotic soy product on fecal microbiota and its association with cardiovascular risk factors in a animal model |
title_full_unstemmed |
Influence of a probiotic soy product on fecal microbiota and its association with cardiovascular risk factors in a animal model |
title_sort |
Influence of a probiotic soy product on fecal microbiota and its association with cardiovascular risk factors in a animal model |
author |
Cavallini, Daniela Cardoso Umbelino [UNESP] |
author_facet |
Cavallini, Daniela Cardoso Umbelino [UNESP] Suzuki, Juliana Y. [UNESP] Abdalla, Dulcinéia S P Vendramini, Regina C. [UNESP] Pauly-silveira, Nadiége D [UNESP] Roselino, Mariana N [UNESP] Pinto, Roseli Ap. [UNESP] Rossi, Elizeu Antonio [UNESP] |
author_role |
author |
author2 |
Suzuki, Juliana Y. [UNESP] Abdalla, Dulcinéia S P Vendramini, Regina C. [UNESP] Pauly-silveira, Nadiége D [UNESP] Roselino, Mariana N [UNESP] Pinto, Roseli Ap. [UNESP] Rossi, Elizeu Antonio [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Cavallini, Daniela Cardoso Umbelino [UNESP] Suzuki, Juliana Y. [UNESP] Abdalla, Dulcinéia S P Vendramini, Regina C. [UNESP] Pauly-silveira, Nadiége D [UNESP] Roselino, Mariana N [UNESP] Pinto, Roseli Ap. [UNESP] Rossi, Elizeu Antonio [UNESP] |
dc.subject.por.fl_str_mv |
Probiotics Enterococcus faecium CRL 183 Fecal microbiota Lipid parameters |
topic |
Probiotics Enterococcus faecium CRL 183 Fecal microbiota Lipid parameters |
description |
Background: Previous work showed that daily ingestion of an aqueous soy extract fermented with Enterococcus faecium CRL 183 and Lactobacillus helveticus 416, supplemented or not with isoflavones, reduced the total cholesterol and non-HDL-cholesterol levels, increased the high-density lipoprotein (HDL) concentration and inhibited the raising of autoantibody against oxidized low-density lipoprotein (ox-LDL Ab) and the development of atherosclerotic lesions. Objective: The aim of this study was to characterize the fecal microbiota in order to investigate the possible correlation between fecal microbiota, serum lipid parameters and atherosclerotic lesion development in rabbits with induced hypercholesterolemia, that ingested the aqueous soy extract fermented with Enterococcus faecium CRL 183 and Lactobacillus helveticus 416. Methods: The rabbits were randomly allocated to five experimental groups (n = 6): control (C), hypercholesterolemic (H), hypercholesterolemic plus unfermented soy product (HUF), hypercholesterolemic plus fermented soy product (HF) and hypercholesterolemic plus isoflavone-supplemented fermented soy product (HIF). Lipid parameters and microbiota composition were analyzed on days 0 and 60 of the treatment and the atherosclerotic lesions were quantified at the end of the experiment. The fecal microbiota was characterized by enumerating the Lactobacillus spp., Bifidobacterium spp., Enterococcus spp., Enterobacteria and Clostridium spp. populations. Results: After 60 days of the experiment, intake of the probiotic soy product was correlated with significant increases (P < 0.05) on Lactobacillus spp., Bifidobacterium spp. and Enterococcus spp. and a decrease in the Enterobacteria population. A strong correlation was observed between microbiota composition and lipid profile. Populations of Enterococcus spp., Lactobacillus spp. and Bifidobacterium spp. were negatively correlated with total cholesterol, non-HDL-cholesterol, autoantibodies against oxidized LDL (oxLDL Ab) and lesion size. HDL-C levels were positively correlated with Lactobacillus spp., Bifidobacterium spp., and Enterococcus spp. populations. Conclusion: In conclusion, daily ingestion of the probiotic soy product, supplemented or not with isoflavones, may contribute to a beneficial balance of the fecal microbiota and this modulation is associated with an improved cholesterol profile and inhibition of atherosclerotic lesion development. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011 2016-01-28T16:56:13Z 2016-01-28T16:56:13Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://link.springer.com/article/10.1186%2F1476-511X-10-126 Lipids in health and disease, v. 10, n. 126, p. 1-9, 2011. 1476-511X http://hdl.handle.net/11449/133699 10.1186/1476-511X-10-126 ISSN1476-511X-2011-10-126-1-9.pdf 4631598241372861 3242858535763793 9905593337419625 8546420799947783 7605568238887469 1194360710417891 |
url |
http://link.springer.com/article/10.1186%2F1476-511X-10-126 http://hdl.handle.net/11449/133699 |
identifier_str_mv |
Lipids in health and disease, v. 10, n. 126, p. 1-9, 2011. 1476-511X 10.1186/1476-511X-10-126 ISSN1476-511X-2011-10-126-1-9.pdf 4631598241372861 3242858535763793 9905593337419625 8546420799947783 7605568238887469 1194360710417891 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Lipids in health and disease 2.663 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1-9 application/pdf |
dc.source.none.fl_str_mv |
Currículo Lattes reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128930450767872 |