Identificação de espécies e genótipos de Cryptosporidium em bovinos leiteiros no Brasil
Autor(a) principal: | |
---|---|
Data de Publicação: | 2012 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng por |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://hdl.handle.net/11449/227219 |
Resumo: | In this study, we identified Cryptosporidium species and genotypes present in dairy cattle in the central region of São Paulo state, Brazil. Fecal specimens were collected from 200 animals (100 calves and 100 cows) in ten dairy farms. Fecal samples were examined using microscopic examination (ME), enzyme immunoassay (EIA) and polymerase chain reaction (PCR). Cryptosporidium species and genotypes were determined by restriction fragment length polymorphism (RFLP) or DNA sequencing analysis of the SSU-rRNA and GP60 genes. The occurrence of Cryptosporidium spp. infection was 14% (28/200). The occurrence in calves (26%) was significantly higher than in cows (2%). Of the 27 Cryptosporidium-positive specimens submitted to genotyping, C. andersoni was identified in 23 (85.1%), C. bovis in three (11.1%), and the zoonotic C. parvum subtype IIaA15G2R1 in one (3.7%). The study demonstrates that Cryptosporidium spp. infection was common and widespread in dairy cattle in this region and that calves have a high prevalence of C. andersoni. Furthermore, the presence of C. parvum subtype IIaA15G2R1 indicates that dairy calves from this region should be considered a potential source of zoonotic Cryptosporidium oocysts. |
id |
UNSP_189204e52333de5c5b3ad2006964ed82 |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/227219 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Identificação de espécies e genótipos de Cryptosporidium em bovinos leiteiros no BrasilIdentification of Cryptosporidium species and genotypes in dairy cattle in BrazilCattleCryptosporidium spp.GenotypingPCR-RFLPSequencingIn this study, we identified Cryptosporidium species and genotypes present in dairy cattle in the central region of São Paulo state, Brazil. Fecal specimens were collected from 200 animals (100 calves and 100 cows) in ten dairy farms. Fecal samples were examined using microscopic examination (ME), enzyme immunoassay (EIA) and polymerase chain reaction (PCR). Cryptosporidium species and genotypes were determined by restriction fragment length polymorphism (RFLP) or DNA sequencing analysis of the SSU-rRNA and GP60 genes. The occurrence of Cryptosporidium spp. infection was 14% (28/200). The occurrence in calves (26%) was significantly higher than in cows (2%). Of the 27 Cryptosporidium-positive specimens submitted to genotyping, C. andersoni was identified in 23 (85.1%), C. bovis in three (11.1%), and the zoonotic C. parvum subtype IIaA15G2R1 in one (3.7%). The study demonstrates that Cryptosporidium spp. infection was common and widespread in dairy cattle in this region and that calves have a high prevalence of C. andersoni. Furthermore, the presence of C. parvum subtype IIaA15G2R1 indicates that dairy calves from this region should be considered a potential source of zoonotic Cryptosporidium oocysts.Laboratório de Enfermidades Parasitárias Departamento de Clínica Veterinária Universidade Estadual Paulista - UNESP, Botucatu, SPLaboratório de Diagnóstico Molecular Departamento de Microbiologia-Imunologia Instituto de Biociências, Universidade Estadual Paulista - UNESP, Botucatu, SPLaboratório de Enfermidades Parasitárias Departamento de Clínica Veterinária Universidade Estadual Paulista - UNESP, Botucatu, SPLaboratório de Diagnóstico Molecular Departamento de Microbiologia-Imunologia Instituto de Biociências, Universidade Estadual Paulista - UNESP, Botucatu, SPUniversidade Estadual Paulista (UNESP)Paz e Silva, Flavio Medeiros [UNESP]Lopes, Raimundo Souza [UNESP]Araújo-Junior, João Pessoa [UNESP]2022-04-29T07:12:10Z2022-04-29T07:12:10Z2012-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article22-28Revista Brasileira de Parasitologia Veterinaria, v. 22, n. 1, p. 22-28, 2012.0103-846Xhttp://hdl.handle.net/11449/2272192-s2.0-84885476538Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengporRevista Brasileira de Parasitologia Veterinariainfo:eu-repo/semantics/openAccess2022-04-29T07:12:10Zoai:repositorio.unesp.br:11449/227219Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T13:57:01.651873Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Identificação de espécies e genótipos de Cryptosporidium em bovinos leiteiros no Brasil Identification of Cryptosporidium species and genotypes in dairy cattle in Brazil |
title |
Identificação de espécies e genótipos de Cryptosporidium em bovinos leiteiros no Brasil |
spellingShingle |
Identificação de espécies e genótipos de Cryptosporidium em bovinos leiteiros no Brasil Paz e Silva, Flavio Medeiros [UNESP] Cattle Cryptosporidium spp. Genotyping PCR-RFLP Sequencing |
title_short |
Identificação de espécies e genótipos de Cryptosporidium em bovinos leiteiros no Brasil |
title_full |
Identificação de espécies e genótipos de Cryptosporidium em bovinos leiteiros no Brasil |
title_fullStr |
Identificação de espécies e genótipos de Cryptosporidium em bovinos leiteiros no Brasil |
title_full_unstemmed |
Identificação de espécies e genótipos de Cryptosporidium em bovinos leiteiros no Brasil |
title_sort |
Identificação de espécies e genótipos de Cryptosporidium em bovinos leiteiros no Brasil |
author |
Paz e Silva, Flavio Medeiros [UNESP] |
author_facet |
Paz e Silva, Flavio Medeiros [UNESP] Lopes, Raimundo Souza [UNESP] Araújo-Junior, João Pessoa [UNESP] |
author_role |
author |
author2 |
Lopes, Raimundo Souza [UNESP] Araújo-Junior, João Pessoa [UNESP] |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Paz e Silva, Flavio Medeiros [UNESP] Lopes, Raimundo Souza [UNESP] Araújo-Junior, João Pessoa [UNESP] |
dc.subject.por.fl_str_mv |
Cattle Cryptosporidium spp. Genotyping PCR-RFLP Sequencing |
topic |
Cattle Cryptosporidium spp. Genotyping PCR-RFLP Sequencing |
description |
In this study, we identified Cryptosporidium species and genotypes present in dairy cattle in the central region of São Paulo state, Brazil. Fecal specimens were collected from 200 animals (100 calves and 100 cows) in ten dairy farms. Fecal samples were examined using microscopic examination (ME), enzyme immunoassay (EIA) and polymerase chain reaction (PCR). Cryptosporidium species and genotypes were determined by restriction fragment length polymorphism (RFLP) or DNA sequencing analysis of the SSU-rRNA and GP60 genes. The occurrence of Cryptosporidium spp. infection was 14% (28/200). The occurrence in calves (26%) was significantly higher than in cows (2%). Of the 27 Cryptosporidium-positive specimens submitted to genotyping, C. andersoni was identified in 23 (85.1%), C. bovis in three (11.1%), and the zoonotic C. parvum subtype IIaA15G2R1 in one (3.7%). The study demonstrates that Cryptosporidium spp. infection was common and widespread in dairy cattle in this region and that calves have a high prevalence of C. andersoni. Furthermore, the presence of C. parvum subtype IIaA15G2R1 indicates that dairy calves from this region should be considered a potential source of zoonotic Cryptosporidium oocysts. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-01-01 2022-04-29T07:12:10Z 2022-04-29T07:12:10Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Revista Brasileira de Parasitologia Veterinaria, v. 22, n. 1, p. 22-28, 2012. 0103-846X http://hdl.handle.net/11449/227219 2-s2.0-84885476538 |
identifier_str_mv |
Revista Brasileira de Parasitologia Veterinaria, v. 22, n. 1, p. 22-28, 2012. 0103-846X 2-s2.0-84885476538 |
url |
http://hdl.handle.net/11449/227219 |
dc.language.iso.fl_str_mv |
eng por |
language |
eng por |
dc.relation.none.fl_str_mv |
Revista Brasileira de Parasitologia Veterinaria |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
22-28 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128294319554560 |