Plasma membrane permeabilization to explain erythrosine B phototoxicity on in vitro breast cancer cell models
Autor(a) principal: | |
---|---|
Data de Publicação: | 2021 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.jphotobiol.2021.112297 http://hdl.handle.net/11449/222361 |
Resumo: | Lipid oxidation is ubiquitous in cell life under oxygen and essential for photodynamic therapy (PDT) of carcinomas. However, the mechanisms underlying lipid oxidation in rather complex systems such as plasma membranes remain elusive. Herein, Langmuir monolayers were assembled with the lipid extract of glandular breast cancer (MCF7) cells and used to probe the molecular interactions allowing adsorption of the photosensitizer (PS) erythrosine B and subsequent photooxidation outcomes. Surface pressure (π) versus area (cm2/mL) isotherms of MCF7 lipid extract shifted to larger areas upon erythrosine incorporation, driven by secondary interactions that affected the orientation of the carbonyl groups and lipid chain organization. Light-irradiation increased the surface area of the MCF7 lipid extract monolayer containing erythrosine owing to the lipid hydroperoxidation, which may further undergo decomposition, resulting in the chain cleavage of phospholipids and membrane permeabilization. Incorporation of erythrosine by MCF7 cells induced slight toxic effects on in vitro assays, differently of the severe phototoxicity caused by light-irradiation, which significantly decreased cell viability by more than 75% at 2.5 × 10−6 mol/L of erythrosine incubated for 3 and 24 h, reaching nearly 90% at 48 h of incubation. The origin of the phototoxic effects is in the rupture of the plasma membrane shown by the frontal (FSC) and side (SSC) light scattering of flow cytometry. Consistent with hydroperoxide decomposition, membrane permeabilization was also confirmed by cleaved lipids detected in mass spectrometry and subsidizes the necrotic pathway of cell death. |
id |
UNSP_18f726cc0c76e83742b47c47c862c319 |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/222361 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Plasma membrane permeabilization to explain erythrosine B phototoxicity on in vitro breast cancer cell modelsErythrosine BGlandular breast cancer (MCF7) cellsLangmuir filmsPhotodynamic therapy (PDT)Lipid oxidation is ubiquitous in cell life under oxygen and essential for photodynamic therapy (PDT) of carcinomas. However, the mechanisms underlying lipid oxidation in rather complex systems such as plasma membranes remain elusive. Herein, Langmuir monolayers were assembled with the lipid extract of glandular breast cancer (MCF7) cells and used to probe the molecular interactions allowing adsorption of the photosensitizer (PS) erythrosine B and subsequent photooxidation outcomes. Surface pressure (π) versus area (cm2/mL) isotherms of MCF7 lipid extract shifted to larger areas upon erythrosine incorporation, driven by secondary interactions that affected the orientation of the carbonyl groups and lipid chain organization. Light-irradiation increased the surface area of the MCF7 lipid extract monolayer containing erythrosine owing to the lipid hydroperoxidation, which may further undergo decomposition, resulting in the chain cleavage of phospholipids and membrane permeabilization. Incorporation of erythrosine by MCF7 cells induced slight toxic effects on in vitro assays, differently of the severe phototoxicity caused by light-irradiation, which significantly decreased cell viability by more than 75% at 2.5 × 10−6 mol/L of erythrosine incubated for 3 and 24 h, reaching nearly 90% at 48 h of incubation. The origin of the phototoxic effects is in the rupture of the plasma membrane shown by the frontal (FSC) and side (SSC) light scattering of flow cytometry. Consistent with hydroperoxide decomposition, membrane permeabilization was also confirmed by cleaved lipids detected in mass spectrometry and subsidizes the necrotic pathway of cell death.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)São Paulo State University (UNESP) School of Sciences Humanities and LanguagesIFSC São Carlos Institute of Physics University of São Paulo (USP)INNOVARE Biomarkers Laboratory School of Pharmaceutical Sciences University of CampinasSão Paulo State University (UNESP) Institute of Biosciences Letters and Exact SciencesSão Paulo State University (UNESP) School of Sciences Humanities and LanguagesSão Paulo State University (UNESP) Institute of Biosciences Letters and Exact SciencesFAPESP: 2016/20576-2FAPESP: 2018/14692-5FAPESP: 2018/16713-0FAPESP: 2018/22214-6CNPq: 403713/2016-1FAPESP: EMU 2013/14262-7Universidade Estadual Paulista (UNESP)Universidade de São Paulo (USP)Universidade Estadual de Campinas (UNICAMP)Bistaffa, Maria J. [UNESP]Camacho, Sabrina A. [UNESP]Melo, Carlos F.O.R. [UNESP]Catharino, Rodrigo R.Toledo, Karina A. [UNESP]Aoki, Pedro H.B. [UNESP]2022-04-28T19:44:14Z2022-04-28T19:44:14Z2021-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.jphotobiol.2021.112297Journal of Photochemistry and Photobiology B: Biology, v. 223.1873-26821011-1344http://hdl.handle.net/11449/22236110.1016/j.jphotobiol.2021.1122972-s2.0-85114337337Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Photochemistry and Photobiology B: Biologyinfo:eu-repo/semantics/openAccess2022-04-28T19:44:14Zoai:repositorio.unesp.br:11449/222361Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462022-04-28T19:44:14Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Plasma membrane permeabilization to explain erythrosine B phototoxicity on in vitro breast cancer cell models |
title |
Plasma membrane permeabilization to explain erythrosine B phototoxicity on in vitro breast cancer cell models |
spellingShingle |
Plasma membrane permeabilization to explain erythrosine B phototoxicity on in vitro breast cancer cell models Bistaffa, Maria J. [UNESP] Erythrosine B Glandular breast cancer (MCF7) cells Langmuir films Photodynamic therapy (PDT) |
title_short |
Plasma membrane permeabilization to explain erythrosine B phototoxicity on in vitro breast cancer cell models |
title_full |
Plasma membrane permeabilization to explain erythrosine B phototoxicity on in vitro breast cancer cell models |
title_fullStr |
Plasma membrane permeabilization to explain erythrosine B phototoxicity on in vitro breast cancer cell models |
title_full_unstemmed |
Plasma membrane permeabilization to explain erythrosine B phototoxicity on in vitro breast cancer cell models |
title_sort |
Plasma membrane permeabilization to explain erythrosine B phototoxicity on in vitro breast cancer cell models |
author |
Bistaffa, Maria J. [UNESP] |
author_facet |
Bistaffa, Maria J. [UNESP] Camacho, Sabrina A. [UNESP] Melo, Carlos F.O.R. [UNESP] Catharino, Rodrigo R. Toledo, Karina A. [UNESP] Aoki, Pedro H.B. [UNESP] |
author_role |
author |
author2 |
Camacho, Sabrina A. [UNESP] Melo, Carlos F.O.R. [UNESP] Catharino, Rodrigo R. Toledo, Karina A. [UNESP] Aoki, Pedro H.B. [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Universidade de São Paulo (USP) Universidade Estadual de Campinas (UNICAMP) |
dc.contributor.author.fl_str_mv |
Bistaffa, Maria J. [UNESP] Camacho, Sabrina A. [UNESP] Melo, Carlos F.O.R. [UNESP] Catharino, Rodrigo R. Toledo, Karina A. [UNESP] Aoki, Pedro H.B. [UNESP] |
dc.subject.por.fl_str_mv |
Erythrosine B Glandular breast cancer (MCF7) cells Langmuir films Photodynamic therapy (PDT) |
topic |
Erythrosine B Glandular breast cancer (MCF7) cells Langmuir films Photodynamic therapy (PDT) |
description |
Lipid oxidation is ubiquitous in cell life under oxygen and essential for photodynamic therapy (PDT) of carcinomas. However, the mechanisms underlying lipid oxidation in rather complex systems such as plasma membranes remain elusive. Herein, Langmuir monolayers were assembled with the lipid extract of glandular breast cancer (MCF7) cells and used to probe the molecular interactions allowing adsorption of the photosensitizer (PS) erythrosine B and subsequent photooxidation outcomes. Surface pressure (π) versus area (cm2/mL) isotherms of MCF7 lipid extract shifted to larger areas upon erythrosine incorporation, driven by secondary interactions that affected the orientation of the carbonyl groups and lipid chain organization. Light-irradiation increased the surface area of the MCF7 lipid extract monolayer containing erythrosine owing to the lipid hydroperoxidation, which may further undergo decomposition, resulting in the chain cleavage of phospholipids and membrane permeabilization. Incorporation of erythrosine by MCF7 cells induced slight toxic effects on in vitro assays, differently of the severe phototoxicity caused by light-irradiation, which significantly decreased cell viability by more than 75% at 2.5 × 10−6 mol/L of erythrosine incubated for 3 and 24 h, reaching nearly 90% at 48 h of incubation. The origin of the phototoxic effects is in the rupture of the plasma membrane shown by the frontal (FSC) and side (SSC) light scattering of flow cytometry. Consistent with hydroperoxide decomposition, membrane permeabilization was also confirmed by cleaved lipids detected in mass spectrometry and subsidizes the necrotic pathway of cell death. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-10-01 2022-04-28T19:44:14Z 2022-04-28T19:44:14Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.jphotobiol.2021.112297 Journal of Photochemistry and Photobiology B: Biology, v. 223. 1873-2682 1011-1344 http://hdl.handle.net/11449/222361 10.1016/j.jphotobiol.2021.112297 2-s2.0-85114337337 |
url |
http://dx.doi.org/10.1016/j.jphotobiol.2021.112297 http://hdl.handle.net/11449/222361 |
identifier_str_mv |
Journal of Photochemistry and Photobiology B: Biology, v. 223. 1873-2682 1011-1344 10.1016/j.jphotobiol.2021.112297 2-s2.0-85114337337 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Photochemistry and Photobiology B: Biology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1803650347872288768 |