Dry matter intake, performance, carcass traits and expression of genes of muscle protein metabolism in cattle fed increasing levels of de-oiled wet distillers grains

Detalhes bibliográficos
Autor(a) principal: Ferreira, Mateus [UNESP]
Data de Publicação: 2020
Outros Autores: Niehues, Maria Betania [UNESP], Tomaz, Lais Aquino [UNESP], Baldassini, Welder [UNESP], Ladeira, Marcio, Arrigoni, Mario [UNESP], Martins, Cyntia Ludovico [UNESP], Gionbelli, Tathyane, Paulino, Pedro, Machado Neto, Otavio R. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.anifeedsci.2020.114627
http://hdl.handle.net/11449/210527
Resumo: The supply of we distillers grains (WDG) has been growing in Brazil, with the increase in corn ethanol production. Thus, the aim of this study was to evaluate the effect of de-oiled corn WDG in the diet of feedlot cattle on DMI, performance, carcass traits, meat quality and expression of genes involved in the muscle protein metabolism. One hundred non-castrated F1 Angus-Nellore bulls, aged 20-24 months, with average IBW of 369.58 +/- 49.17 kg, were used in a randomized complete block design. The animals were fed diets containing 0, 150, 300 and 450 g/kg de-oiled WDG (40 g/kg ofEE) replacing corn and soybean meal. After 129 days on feed, the animals were slaughtered and LT muscle samples were collected for molecular biology assays. Muscle protein metabolism genes such as eIF4, IGFR1, mTOR, GSK3f3 and P70S6K were investigated by RTqPCR. Data were analyzed using polynomial contrasts (linear, quadratic and control versus WDG). The inclusion of by-product improved DMI and FBW (P < 0.05). A tendency (P < 0.10) to a quadratic effect was observed on DMI and FBW, with greater values for animals fed diet with 150 and 300 g/kg of WDG. The animals fed WDG presented greater HCW and CCW (P < 0.05), and there was a quadratic effect (P < 0.05) for the REA, with greater values for the animals fed diet with 300 g/kg of WDG. Moreover, no differences were observed in BFT and carcass pH, and the meat quality traits were similar (P > 0.05) among treatments (SF, CL, ILC, MFI, and WHC). However, the inclusion of WDG led to a reduction in the expression of eIF4, mTOR and IGFR1 genes (P < 0.05). Overall, meat quality traits were not affected by nutritional treatments. Although increased levels of de-oiled WDG influenced DMI, FBW, carcass weights and REA (an indicator of muscle deposition) in response to feeding, it did not produce concerted changes in genes of LT related to muscle protein metabolism.
id UNSP_191296c583e3fc0232e963244f7c6368
oai_identifier_str oai:repositorio.unesp.br:11449/210527
network_acronym_str UNSP
network_name_str Repositório Institucional da UNESP
repository_id_str 2946
spelling Dry matter intake, performance, carcass traits and expression of genes of muscle protein metabolism in cattle fed increasing levels of de-oiled wet distillers grainsAnimal nutritionbeef cattlefeedlotmeat qualitymolecular biologyThe supply of we distillers grains (WDG) has been growing in Brazil, with the increase in corn ethanol production. Thus, the aim of this study was to evaluate the effect of de-oiled corn WDG in the diet of feedlot cattle on DMI, performance, carcass traits, meat quality and expression of genes involved in the muscle protein metabolism. One hundred non-castrated F1 Angus-Nellore bulls, aged 20-24 months, with average IBW of 369.58 +/- 49.17 kg, were used in a randomized complete block design. The animals were fed diets containing 0, 150, 300 and 450 g/kg de-oiled WDG (40 g/kg ofEE) replacing corn and soybean meal. After 129 days on feed, the animals were slaughtered and LT muscle samples were collected for molecular biology assays. Muscle protein metabolism genes such as eIF4, IGFR1, mTOR, GSK3f3 and P70S6K were investigated by RTqPCR. Data were analyzed using polynomial contrasts (linear, quadratic and control versus WDG). The inclusion of by-product improved DMI and FBW (P < 0.05). A tendency (P < 0.10) to a quadratic effect was observed on DMI and FBW, with greater values for animals fed diet with 150 and 300 g/kg of WDG. The animals fed WDG presented greater HCW and CCW (P < 0.05), and there was a quadratic effect (P < 0.05) for the REA, with greater values for the animals fed diet with 300 g/kg of WDG. Moreover, no differences were observed in BFT and carcass pH, and the meat quality traits were similar (P > 0.05) among treatments (SF, CL, ILC, MFI, and WHC). However, the inclusion of WDG led to a reduction in the expression of eIF4, mTOR and IGFR1 genes (P < 0.05). Overall, meat quality traits were not affected by nutritional treatments. Although increased levels of de-oiled WDG influenced DMI, FBW, carcass weights and REA (an indicator of muscle deposition) in response to feeding, it did not produce concerted changes in genes of LT related to muscle protein metabolism.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Sao Paulo State Univ, Sch Agr & Veterinarian Sci, BR-14884900 Jaboticabal, SP, BrazilSao Paulo State Univ, Sch Vet Med & Anim Sci, BR-18618681 Botucatu, SP, BrazilUniv Fed Lavras, Anim Sci Dept, BR-37200000 Lavras, MG, BrazilCargill Anim Nutr, BR-13091611 Campinas, SP, BrazilSao Paulo State Univ, Sch Agr & Veterinarian Sci, BR-14884900 Jaboticabal, SP, BrazilSao Paulo State Univ, Sch Vet Med & Anim Sci, BR-18618681 Botucatu, SP, BrazilFAPESP: 2016/04478-0FAPESP: 2017/22923-4FAPESP: 2018/00981-5CAPES: 001Elsevier B.V.Universidade Estadual Paulista (Unesp)Universidade Federal de Lavras (UFLA)Cargill Anim NutrFerreira, Mateus [UNESP]Niehues, Maria Betania [UNESP]Tomaz, Lais Aquino [UNESP]Baldassini, Welder [UNESP]Ladeira, MarcioArrigoni, Mario [UNESP]Martins, Cyntia Ludovico [UNESP]Gionbelli, TathyanePaulino, PedroMachado Neto, Otavio R. [UNESP]2021-06-25T19:36:24Z2021-06-25T19:36:24Z2020-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article11http://dx.doi.org/10.1016/j.anifeedsci.2020.114627Animal Feed Science And Technology. Amsterdam: Elsevier, v. 269, 11 p., 2020.0377-8401http://hdl.handle.net/11449/21052710.1016/j.anifeedsci.2020.114627WOS:000582674200012Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAnimal Feed Science And Technologyinfo:eu-repo/semantics/openAccess2024-09-09T13:00:56Zoai:repositorio.unesp.br:11449/210527Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-09T13:00:56Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Dry matter intake, performance, carcass traits and expression of genes of muscle protein metabolism in cattle fed increasing levels of de-oiled wet distillers grains
title Dry matter intake, performance, carcass traits and expression of genes of muscle protein metabolism in cattle fed increasing levels of de-oiled wet distillers grains
spellingShingle Dry matter intake, performance, carcass traits and expression of genes of muscle protein metabolism in cattle fed increasing levels of de-oiled wet distillers grains
Ferreira, Mateus [UNESP]
Animal nutrition
beef cattle
feedlot
meat quality
molecular biology
title_short Dry matter intake, performance, carcass traits and expression of genes of muscle protein metabolism in cattle fed increasing levels of de-oiled wet distillers grains
title_full Dry matter intake, performance, carcass traits and expression of genes of muscle protein metabolism in cattle fed increasing levels of de-oiled wet distillers grains
title_fullStr Dry matter intake, performance, carcass traits and expression of genes of muscle protein metabolism in cattle fed increasing levels of de-oiled wet distillers grains
title_full_unstemmed Dry matter intake, performance, carcass traits and expression of genes of muscle protein metabolism in cattle fed increasing levels of de-oiled wet distillers grains
title_sort Dry matter intake, performance, carcass traits and expression of genes of muscle protein metabolism in cattle fed increasing levels of de-oiled wet distillers grains
author Ferreira, Mateus [UNESP]
author_facet Ferreira, Mateus [UNESP]
Niehues, Maria Betania [UNESP]
Tomaz, Lais Aquino [UNESP]
Baldassini, Welder [UNESP]
Ladeira, Marcio
Arrigoni, Mario [UNESP]
Martins, Cyntia Ludovico [UNESP]
Gionbelli, Tathyane
Paulino, Pedro
Machado Neto, Otavio R. [UNESP]
author_role author
author2 Niehues, Maria Betania [UNESP]
Tomaz, Lais Aquino [UNESP]
Baldassini, Welder [UNESP]
Ladeira, Marcio
Arrigoni, Mario [UNESP]
Martins, Cyntia Ludovico [UNESP]
Gionbelli, Tathyane
Paulino, Pedro
Machado Neto, Otavio R. [UNESP]
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Universidade Federal de Lavras (UFLA)
Cargill Anim Nutr
dc.contributor.author.fl_str_mv Ferreira, Mateus [UNESP]
Niehues, Maria Betania [UNESP]
Tomaz, Lais Aquino [UNESP]
Baldassini, Welder [UNESP]
Ladeira, Marcio
Arrigoni, Mario [UNESP]
Martins, Cyntia Ludovico [UNESP]
Gionbelli, Tathyane
Paulino, Pedro
Machado Neto, Otavio R. [UNESP]
dc.subject.por.fl_str_mv Animal nutrition
beef cattle
feedlot
meat quality
molecular biology
topic Animal nutrition
beef cattle
feedlot
meat quality
molecular biology
description The supply of we distillers grains (WDG) has been growing in Brazil, with the increase in corn ethanol production. Thus, the aim of this study was to evaluate the effect of de-oiled corn WDG in the diet of feedlot cattle on DMI, performance, carcass traits, meat quality and expression of genes involved in the muscle protein metabolism. One hundred non-castrated F1 Angus-Nellore bulls, aged 20-24 months, with average IBW of 369.58 +/- 49.17 kg, were used in a randomized complete block design. The animals were fed diets containing 0, 150, 300 and 450 g/kg de-oiled WDG (40 g/kg ofEE) replacing corn and soybean meal. After 129 days on feed, the animals were slaughtered and LT muscle samples were collected for molecular biology assays. Muscle protein metabolism genes such as eIF4, IGFR1, mTOR, GSK3f3 and P70S6K were investigated by RTqPCR. Data were analyzed using polynomial contrasts (linear, quadratic and control versus WDG). The inclusion of by-product improved DMI and FBW (P < 0.05). A tendency (P < 0.10) to a quadratic effect was observed on DMI and FBW, with greater values for animals fed diet with 150 and 300 g/kg of WDG. The animals fed WDG presented greater HCW and CCW (P < 0.05), and there was a quadratic effect (P < 0.05) for the REA, with greater values for the animals fed diet with 300 g/kg of WDG. Moreover, no differences were observed in BFT and carcass pH, and the meat quality traits were similar (P > 0.05) among treatments (SF, CL, ILC, MFI, and WHC). However, the inclusion of WDG led to a reduction in the expression of eIF4, mTOR and IGFR1 genes (P < 0.05). Overall, meat quality traits were not affected by nutritional treatments. Although increased levels of de-oiled WDG influenced DMI, FBW, carcass weights and REA (an indicator of muscle deposition) in response to feeding, it did not produce concerted changes in genes of LT related to muscle protein metabolism.
publishDate 2020
dc.date.none.fl_str_mv 2020-11-01
2021-06-25T19:36:24Z
2021-06-25T19:36:24Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.anifeedsci.2020.114627
Animal Feed Science And Technology. Amsterdam: Elsevier, v. 269, 11 p., 2020.
0377-8401
http://hdl.handle.net/11449/210527
10.1016/j.anifeedsci.2020.114627
WOS:000582674200012
url http://dx.doi.org/10.1016/j.anifeedsci.2020.114627
http://hdl.handle.net/11449/210527
identifier_str_mv Animal Feed Science And Technology. Amsterdam: Elsevier, v. 269, 11 p., 2020.
0377-8401
10.1016/j.anifeedsci.2020.114627
WOS:000582674200012
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Animal Feed Science And Technology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 11
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
_version_ 1813546572951060480