Toxicity of β-(1→3,1→6)-ᴅ-glucans produced by diaporthe sp. endophytes on metarhizium anisopliae (metschnikoff) sorokin assessed by conidia germination speed parameter
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.14393/BJ-v36n5a2020-47749 http://hdl.handle.net/11449/199274 |
Resumo: | We have previously reported that β-(1→3,1→6)-ᴅ-glucans produced by endophytes Diaporthe sp. G27-60 and G65-65 (GenBank accession codes JF766998 and JF767007, respectively) are promising anti-proliferation agents against human breast carcinoma (MCF-7) and hepatocellular carcinoma (HepG2-C3A) cells. However, the literature fails to describe the effects of Diaporthe exopolysaccharides (EPS) on eukaryotic healthy cells. The fungus Metarhizium anisopliae has been employed as model-system to evaluate the toxicity of pharmaceutical and agricultural-interest substances, taking into account, among other parameters, the speed of conidia germination. Current study verified the effect of different concentrations of Diaporthe β-glucans on the germination speed of M. anisopliae. Conidia were incubated with β-glucans treatments (50, 200 and 400 μg/mL) at 28ºC, sampled during 24 h and analyzed by light microscopy. At the end of a 24-h incubation, the amount of germinated conidia reached ≈99% for controls and ranged between 97.7 and 98.6% for treatments. Bayesian analysis indicated that Diaporthe glucans had no toxicity on M. anisopliae and the curve of germination occurred as expected for this fungal strain. Considering the validity of filamentous fungi as model-systems, results are important data on the toxicity of endophytic EPS on healthy cells and may be associated with our previous results obtained for these polymers against tumor cells. |
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Toxicity of β-(1→3,1→6)-ᴅ-glucans produced by diaporthe sp. endophytes on metarhizium anisopliae (metschnikoff) sorokin assessed by conidia germination speed parameterToxicidade de β-(1→ 3,1→ 6)-ᴅ-glucanas produzidas por endófitos diaporthe sp. sobre metarhizium anisopliae (metschnikoff) sorokin avaliada pelo parâmetro de velocidade de germinação dos conídiosBayesian analysisEndophytic fungiExopolysaccharideFungal model-systemWe have previously reported that β-(1→3,1→6)-ᴅ-glucans produced by endophytes Diaporthe sp. G27-60 and G65-65 (GenBank accession codes JF766998 and JF767007, respectively) are promising anti-proliferation agents against human breast carcinoma (MCF-7) and hepatocellular carcinoma (HepG2-C3A) cells. However, the literature fails to describe the effects of Diaporthe exopolysaccharides (EPS) on eukaryotic healthy cells. The fungus Metarhizium anisopliae has been employed as model-system to evaluate the toxicity of pharmaceutical and agricultural-interest substances, taking into account, among other parameters, the speed of conidia germination. Current study verified the effect of different concentrations of Diaporthe β-glucans on the germination speed of M. anisopliae. Conidia were incubated with β-glucans treatments (50, 200 and 400 μg/mL) at 28ºC, sampled during 24 h and analyzed by light microscopy. At the end of a 24-h incubation, the amount of germinated conidia reached ≈99% for controls and ranged between 97.7 and 98.6% for treatments. Bayesian analysis indicated that Diaporthe glucans had no toxicity on M. anisopliae and the curve of germination occurred as expected for this fungal strain. Considering the validity of filamentous fungi as model-systems, results are important data on the toxicity of endophytic EPS on healthy cells and may be associated with our previous results obtained for these polymers against tumor cells.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Universidade Estadual de Maringá (UEM)Universidade Católica Dom Bosco (UCDB)University of Georgia (UGA)Faculdade de Ciências e Tecnologia Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP)Escola Superior de Agricultura Luiz de Queiroz (ESALQ/USP)Núcleo Permanente Docente do Programa de Pós-graduação em Biotecnologia Ambiental da Universidade Estadual de Maringá (UEM)Faculdade de Ciências e Tecnologia Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP)CNPq: 311534/2014-7CNPq: 447265/2014-8Universidade Estadual de Maringá (UEM)Universidade Católica Dom Bosco (UCDB)University of Georgia (UGA)Universidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Orlandelli, Ravely Casarottide ALMEIDA, Tiago TognolliLourenco, Daniela Andressa LinoVasconcelos, Ana Flora Dalberto [UNESP]CORRADI DA SILVA, Maria de Lourdes [UNESP]de AZEVEDO, João LúcioPamphile, João Alencar2020-12-12T01:35:25Z2020-12-12T01:35:25Z2020-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1742-1749http://dx.doi.org/10.14393/BJ-v36n5a2020-47749Bioscience Journal, v. 36, n. 5, p. 1742-1749, 2020.1981-31631516-3725http://hdl.handle.net/11449/19927410.14393/BJ-v36n5a2020-477492-s2.0-85089559721Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBioscience Journalinfo:eu-repo/semantics/openAccess2021-10-23T06:37:36Zoai:repositorio.unesp.br:11449/199274Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:13:16.254586Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Toxicity of β-(1→3,1→6)-ᴅ-glucans produced by diaporthe sp. endophytes on metarhizium anisopliae (metschnikoff) sorokin assessed by conidia germination speed parameter Toxicidade de β-(1→ 3,1→ 6)-ᴅ-glucanas produzidas por endófitos diaporthe sp. sobre metarhizium anisopliae (metschnikoff) sorokin avaliada pelo parâmetro de velocidade de germinação dos conídios |
title |
Toxicity of β-(1→3,1→6)-ᴅ-glucans produced by diaporthe sp. endophytes on metarhizium anisopliae (metschnikoff) sorokin assessed by conidia germination speed parameter |
spellingShingle |
Toxicity of β-(1→3,1→6)-ᴅ-glucans produced by diaporthe sp. endophytes on metarhizium anisopliae (metschnikoff) sorokin assessed by conidia germination speed parameter Orlandelli, Ravely Casarotti Bayesian analysis Endophytic fungi Exopolysaccharide Fungal model-system |
title_short |
Toxicity of β-(1→3,1→6)-ᴅ-glucans produced by diaporthe sp. endophytes on metarhizium anisopliae (metschnikoff) sorokin assessed by conidia germination speed parameter |
title_full |
Toxicity of β-(1→3,1→6)-ᴅ-glucans produced by diaporthe sp. endophytes on metarhizium anisopliae (metschnikoff) sorokin assessed by conidia germination speed parameter |
title_fullStr |
Toxicity of β-(1→3,1→6)-ᴅ-glucans produced by diaporthe sp. endophytes on metarhizium anisopliae (metschnikoff) sorokin assessed by conidia germination speed parameter |
title_full_unstemmed |
Toxicity of β-(1→3,1→6)-ᴅ-glucans produced by diaporthe sp. endophytes on metarhizium anisopliae (metschnikoff) sorokin assessed by conidia germination speed parameter |
title_sort |
Toxicity of β-(1→3,1→6)-ᴅ-glucans produced by diaporthe sp. endophytes on metarhizium anisopliae (metschnikoff) sorokin assessed by conidia germination speed parameter |
author |
Orlandelli, Ravely Casarotti |
author_facet |
Orlandelli, Ravely Casarotti de ALMEIDA, Tiago Tognolli Lourenco, Daniela Andressa Lino Vasconcelos, Ana Flora Dalberto [UNESP] CORRADI DA SILVA, Maria de Lourdes [UNESP] de AZEVEDO, João Lúcio Pamphile, João Alencar |
author_role |
author |
author2 |
de ALMEIDA, Tiago Tognolli Lourenco, Daniela Andressa Lino Vasconcelos, Ana Flora Dalberto [UNESP] CORRADI DA SILVA, Maria de Lourdes [UNESP] de AZEVEDO, João Lúcio Pamphile, João Alencar |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual de Maringá (UEM) Universidade Católica Dom Bosco (UCDB) University of Georgia (UGA) Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Orlandelli, Ravely Casarotti de ALMEIDA, Tiago Tognolli Lourenco, Daniela Andressa Lino Vasconcelos, Ana Flora Dalberto [UNESP] CORRADI DA SILVA, Maria de Lourdes [UNESP] de AZEVEDO, João Lúcio Pamphile, João Alencar |
dc.subject.por.fl_str_mv |
Bayesian analysis Endophytic fungi Exopolysaccharide Fungal model-system |
topic |
Bayesian analysis Endophytic fungi Exopolysaccharide Fungal model-system |
description |
We have previously reported that β-(1→3,1→6)-ᴅ-glucans produced by endophytes Diaporthe sp. G27-60 and G65-65 (GenBank accession codes JF766998 and JF767007, respectively) are promising anti-proliferation agents against human breast carcinoma (MCF-7) and hepatocellular carcinoma (HepG2-C3A) cells. However, the literature fails to describe the effects of Diaporthe exopolysaccharides (EPS) on eukaryotic healthy cells. The fungus Metarhizium anisopliae has been employed as model-system to evaluate the toxicity of pharmaceutical and agricultural-interest substances, taking into account, among other parameters, the speed of conidia germination. Current study verified the effect of different concentrations of Diaporthe β-glucans on the germination speed of M. anisopliae. Conidia were incubated with β-glucans treatments (50, 200 and 400 μg/mL) at 28ºC, sampled during 24 h and analyzed by light microscopy. At the end of a 24-h incubation, the amount of germinated conidia reached ≈99% for controls and ranged between 97.7 and 98.6% for treatments. Bayesian analysis indicated that Diaporthe glucans had no toxicity on M. anisopliae and the curve of germination occurred as expected for this fungal strain. Considering the validity of filamentous fungi as model-systems, results are important data on the toxicity of endophytic EPS on healthy cells and may be associated with our previous results obtained for these polymers against tumor cells. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T01:35:25Z 2020-12-12T01:35:25Z 2020-09-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.14393/BJ-v36n5a2020-47749 Bioscience Journal, v. 36, n. 5, p. 1742-1749, 2020. 1981-3163 1516-3725 http://hdl.handle.net/11449/199274 10.14393/BJ-v36n5a2020-47749 2-s2.0-85089559721 |
url |
http://dx.doi.org/10.14393/BJ-v36n5a2020-47749 http://hdl.handle.net/11449/199274 |
identifier_str_mv |
Bioscience Journal, v. 36, n. 5, p. 1742-1749, 2020. 1981-3163 1516-3725 10.14393/BJ-v36n5a2020-47749 2-s2.0-85089559721 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
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Bioscience Journal |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1742-1749 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128482349154304 |