Alterations of protein expression in conditions of copper-deprivation for Paracoccidioides lutzii in the presence of extracellular matrix components

Detalhes bibliográficos
Autor(a) principal: Oliveira, Haroldo Cesar de [UNESP]
Data de Publicação: 2014
Outros Autores: Silva, Julhiany de Fatima da [UNESP], Matsumoto, Marcelo Teruyuki [UNESP], Marcos, Caroline Maria [UNESP], Silva, Roberta Peres da [UNESP], Moraes da Silva, Rosangela Aparecida [UNESP], Veneziano Labate, Monica Teresa, Labate, Carlos Alberto, Fusco Almeida, Ana Marisa [UNESP], Mendes-Giannini, Maria José Soares [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://www.biomedcentral.com/1471-2180/14/302
http://hdl.handle.net/11449/130155
Resumo: Background: Paracoccidioides spp is a fungi genus and the agent of paracoccidioidomycosis. The strategies of infection used by these pathogens involve the expression of proteins related to adaptation to the host, particularly regarding the uptake of micronutrients. This study analyzed the adhesion of Paracoccidioides lutzii during conditions of copper (Cu) and iron (Fe) deprivation, while also evaluating the proteins expressed in conditions of Cu depletion in the presence of four extracellular matrix (ECM) components (laminin, fibronectin and types I and IV collagen).Results: We cultured the P. lutzii in a chemically defined media without Cu and Fe. The fungus was then placed in contact with different ECM components and adhesion was evaluated. A significant increase in binding to all ECM components was observed when the fungus was cultured without Cu; which might be related to some adhesins expression. A proteomic assay was developed and revealed 39 proteins expressed that are involved in processes such as virulence, protein synthesis, metabolism, energy, transcription, transport, stress response and the cell cycle when the fungus was interacting with the ECM components. The up-regulated expression of two important adhesins, enolase and 14-3-3, was observed at the fungal cell wall during the interaction with the ECM components, indicating the role of these proteins in the Paracoccidioides-host interaction.Conclusions: This study is important for determining prospective proteins that may be involved in the interaction of Paracoccidioides with a host. Understanding the adaptive response to different growth conditions, elucidating the processes of adhesion and cell invasion, and identifying the proteins that are differentially expressed during the fungus-host interaction may help elucidate mechanisms used for survival and growth of Paracoccidioides in various human tissues.
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spelling Alterations of protein expression in conditions of copper-deprivation for Paracoccidioides lutzii in the presence of extracellular matrix componentsParacoccidioides sppCopperAdhesionProtein expressionParacoccidioidomycosisBackground: Paracoccidioides spp is a fungi genus and the agent of paracoccidioidomycosis. The strategies of infection used by these pathogens involve the expression of proteins related to adaptation to the host, particularly regarding the uptake of micronutrients. This study analyzed the adhesion of Paracoccidioides lutzii during conditions of copper (Cu) and iron (Fe) deprivation, while also evaluating the proteins expressed in conditions of Cu depletion in the presence of four extracellular matrix (ECM) components (laminin, fibronectin and types I and IV collagen).Results: We cultured the P. lutzii in a chemically defined media without Cu and Fe. The fungus was then placed in contact with different ECM components and adhesion was evaluated. A significant increase in binding to all ECM components was observed when the fungus was cultured without Cu; which might be related to some adhesins expression. A proteomic assay was developed and revealed 39 proteins expressed that are involved in processes such as virulence, protein synthesis, metabolism, energy, transcription, transport, stress response and the cell cycle when the fungus was interacting with the ECM components. The up-regulated expression of two important adhesins, enolase and 14-3-3, was observed at the fungal cell wall during the interaction with the ECM components, indicating the role of these proteins in the Paracoccidioides-host interaction.Conclusions: This study is important for determining prospective proteins that may be involved in the interaction of Paracoccidioides with a host. Understanding the adaptive response to different growth conditions, elucidating the processes of adhesion and cell invasion, and identifying the proteins that are differentially expressed during the fungus-host interaction may help elucidate mechanisms used for survival and growth of Paracoccidioides in various human tissues.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)National Council for Scientific and Technological DevelopmentPADCFCF-Sao Paulo State UniversityUniv Estadual Paulista, UNESP, Lab Micol Clin, Fac Ciencias Farmaceut,Dept Anal Clin, Araraquara, SP, BrazilUniv Sao Paulo, Escola Super Agr Luiz de Queiroz, Dept Genet, Lab Multiusuarios Ctr Genom Func Aplicada Agr &A, Sao Paulo, BrazilUniv Estadual Paulista, UNESP, Lab Micol Clin, Fac Ciencias Farmaceut,Dept Anal Clin, Araraquara, SP, BrazilFAPESP: 2008/56476-5FAPESP: 2009/52046-9FAPESP: 2011/18038-9National Council for Scientific and Technological Development: 473119/2010-2Biomed Central LtdUniversidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Oliveira, Haroldo Cesar de [UNESP]Silva, Julhiany de Fatima da [UNESP]Matsumoto, Marcelo Teruyuki [UNESP]Marcos, Caroline Maria [UNESP]Silva, Roberta Peres da [UNESP]Moraes da Silva, Rosangela Aparecida [UNESP]Veneziano Labate, Monica TeresaLabate, Carlos AlbertoFusco Almeida, Ana Marisa [UNESP]Mendes-Giannini, Maria José Soares [UNESP]2015-11-03T15:29:43Z2015-11-03T15:29:43Z2014-12-13info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1-16application/pdfhttp://www.biomedcentral.com/1471-2180/14/302Bmc Microbiology. London: Biomed Central Ltd, v. 14, 16 p., 2014.1471-2180http://hdl.handle.net/11449/13015510.1186/s12866-014-0302-7WOS:000348166400001WOS000348166400001.pdf0000-0002-8059-0826Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBmc Microbiology2.8291,242info:eu-repo/semantics/openAccess2024-06-21T15:18:58Zoai:repositorio.unesp.br:11449/130155Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:26:38.377286Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Alterations of protein expression in conditions of copper-deprivation for Paracoccidioides lutzii in the presence of extracellular matrix components
title Alterations of protein expression in conditions of copper-deprivation for Paracoccidioides lutzii in the presence of extracellular matrix components
spellingShingle Alterations of protein expression in conditions of copper-deprivation for Paracoccidioides lutzii in the presence of extracellular matrix components
Oliveira, Haroldo Cesar de [UNESP]
Paracoccidioides spp
Copper
Adhesion
Protein expression
Paracoccidioidomycosis
title_short Alterations of protein expression in conditions of copper-deprivation for Paracoccidioides lutzii in the presence of extracellular matrix components
title_full Alterations of protein expression in conditions of copper-deprivation for Paracoccidioides lutzii in the presence of extracellular matrix components
title_fullStr Alterations of protein expression in conditions of copper-deprivation for Paracoccidioides lutzii in the presence of extracellular matrix components
title_full_unstemmed Alterations of protein expression in conditions of copper-deprivation for Paracoccidioides lutzii in the presence of extracellular matrix components
title_sort Alterations of protein expression in conditions of copper-deprivation for Paracoccidioides lutzii in the presence of extracellular matrix components
author Oliveira, Haroldo Cesar de [UNESP]
author_facet Oliveira, Haroldo Cesar de [UNESP]
Silva, Julhiany de Fatima da [UNESP]
Matsumoto, Marcelo Teruyuki [UNESP]
Marcos, Caroline Maria [UNESP]
Silva, Roberta Peres da [UNESP]
Moraes da Silva, Rosangela Aparecida [UNESP]
Veneziano Labate, Monica Teresa
Labate, Carlos Alberto
Fusco Almeida, Ana Marisa [UNESP]
Mendes-Giannini, Maria José Soares [UNESP]
author_role author
author2 Silva, Julhiany de Fatima da [UNESP]
Matsumoto, Marcelo Teruyuki [UNESP]
Marcos, Caroline Maria [UNESP]
Silva, Roberta Peres da [UNESP]
Moraes da Silva, Rosangela Aparecida [UNESP]
Veneziano Labate, Monica Teresa
Labate, Carlos Alberto
Fusco Almeida, Ana Marisa [UNESP]
Mendes-Giannini, Maria José Soares [UNESP]
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Universidade de São Paulo (USP)
dc.contributor.author.fl_str_mv Oliveira, Haroldo Cesar de [UNESP]
Silva, Julhiany de Fatima da [UNESP]
Matsumoto, Marcelo Teruyuki [UNESP]
Marcos, Caroline Maria [UNESP]
Silva, Roberta Peres da [UNESP]
Moraes da Silva, Rosangela Aparecida [UNESP]
Veneziano Labate, Monica Teresa
Labate, Carlos Alberto
Fusco Almeida, Ana Marisa [UNESP]
Mendes-Giannini, Maria José Soares [UNESP]
dc.subject.por.fl_str_mv Paracoccidioides spp
Copper
Adhesion
Protein expression
Paracoccidioidomycosis
topic Paracoccidioides spp
Copper
Adhesion
Protein expression
Paracoccidioidomycosis
description Background: Paracoccidioides spp is a fungi genus and the agent of paracoccidioidomycosis. The strategies of infection used by these pathogens involve the expression of proteins related to adaptation to the host, particularly regarding the uptake of micronutrients. This study analyzed the adhesion of Paracoccidioides lutzii during conditions of copper (Cu) and iron (Fe) deprivation, while also evaluating the proteins expressed in conditions of Cu depletion in the presence of four extracellular matrix (ECM) components (laminin, fibronectin and types I and IV collagen).Results: We cultured the P. lutzii in a chemically defined media without Cu and Fe. The fungus was then placed in contact with different ECM components and adhesion was evaluated. A significant increase in binding to all ECM components was observed when the fungus was cultured without Cu; which might be related to some adhesins expression. A proteomic assay was developed and revealed 39 proteins expressed that are involved in processes such as virulence, protein synthesis, metabolism, energy, transcription, transport, stress response and the cell cycle when the fungus was interacting with the ECM components. The up-regulated expression of two important adhesins, enolase and 14-3-3, was observed at the fungal cell wall during the interaction with the ECM components, indicating the role of these proteins in the Paracoccidioides-host interaction.Conclusions: This study is important for determining prospective proteins that may be involved in the interaction of Paracoccidioides with a host. Understanding the adaptive response to different growth conditions, elucidating the processes of adhesion and cell invasion, and identifying the proteins that are differentially expressed during the fungus-host interaction may help elucidate mechanisms used for survival and growth of Paracoccidioides in various human tissues.
publishDate 2014
dc.date.none.fl_str_mv 2014-12-13
2015-11-03T15:29:43Z
2015-11-03T15:29:43Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.biomedcentral.com/1471-2180/14/302
Bmc Microbiology. London: Biomed Central Ltd, v. 14, 16 p., 2014.
1471-2180
http://hdl.handle.net/11449/130155
10.1186/s12866-014-0302-7
WOS:000348166400001
WOS000348166400001.pdf
0000-0002-8059-0826
url http://www.biomedcentral.com/1471-2180/14/302
http://hdl.handle.net/11449/130155
identifier_str_mv Bmc Microbiology. London: Biomed Central Ltd, v. 14, 16 p., 2014.
1471-2180
10.1186/s12866-014-0302-7
WOS:000348166400001
WOS000348166400001.pdf
0000-0002-8059-0826
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Bmc Microbiology
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1,242
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eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 1-16
application/pdf
dc.publisher.none.fl_str_mv Biomed Central Ltd
publisher.none.fl_str_mv Biomed Central Ltd
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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