Efeitos do treinamento físico aeróbio sobre o processo inflamatório e apoptótico em neurônios hipotalâmicos de controle da fome em camundongos obesos induzidos por dieta hiperlipídica

• Autor(a) principal: Marinho, Rodolfo [UNESP]
• Data de Publicação: 2015
• Tipo de documento: Tese
• Idioma: por
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://hdl.handle.net/11449/139386
• topic: Physical education and training; Educação fisica; Obesidade; Apoptose; Inflamação; Neuronios

Objective: To investigate the role of physical training (PT) in regulating the inflammatory processes, endoplasmic reticulum stress and especially apoptosis in hypothalamic neurons responsible for controlling hunger in diet induce obese mice. Methods: Forty-five Swiss mice were distributed into 3 groups of 15 animals each (n = 15): Control - sedentary animals fed a standard diet for 16 weeks; Obese - sedentary animals that received high fat diet (HFD) for 8 weeks (OB); Obese trained (OBT) - animals that received HFD for 8 weeks, and concomitantly, were subjected to a PT protocol. At the beginning and end of the experiment, morphophysiological variables, food intake and molecular biology were evaluated. The TF protocol consisted of treadmill running for 1 h, 5 days / week for 8 weeks, at 60% of Pmax of the animals. Twenty-four hours after the last session of the PT, the extraction of hypothalamus was done for analysis of the level, phosphorylation and / or association of, JAK2, STAT3, TNF-α, IL- 6, IL-1β, JNK, IKK, TLR4 and MyDD88, Bcl-2, Bax, Casp9, Casp8, FADD, APAF1, PERK, eIF2α, NPY and POMC by immunoblot, immunoprecipitation and/or RT-PCR. The TUNEL technique was performed to evaluated apoptosis. In addition, the activation of macrophages was analyzed by labeling with F4/80. Results: DHL was able to increase total body weight, Lee's index, blood glucose and total cholesterol levels and LDL in obese animals compared to the control animals. Rather, PT was able to attenuate these aspects. PT increased phosphorylation of the proteins of leptin signaling pathway (p- JAK2/p-STAT3) in hypothalamic neurons of obese mice compared to not exercised mice. These findings were accompanied by lower content of TNF-α, IL-6, IL-1β and TLR4 and reduced phosphorylation of JNK and IKK trained in the obese animals compared to the obese animals which have remained inactive during the same period. In the same way, it can be observed more macrophage...