Efeito antimicrobiano e citotóxico do óleo essencial de Cymbopogon citratus sobre células odontoblastóides
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://www.robrac.org.br/seer/index.php/ROBRAC/article/view/438 http://hdl.handle.net/11449/125591 |
Resumo: | The aim of this study was to evaluate the antimicrobial and cytotoxic effect of essential oil (EO) of lemon grass (Cymbopogon citratus). From the agar diffusion method, different concentrations of EO (0.135%, 0.2% and 1%), and control solutions (chlorhexidine (Chx), distilled water (Ad) and cereal alcohol (Ac)) were applied on cultures of Candida albicans (C.a), Streptococcus mutans (S.m), Streptococcus sobrinus (S.sob) and Lactobacillus acidophilus (L.a). For C.a, S.m and S.sob, the largest inhibition zones in descending order were: Chx, Ac and EO 1%, while the latter two were statistically similar (Mann-Whitney, p> 0.05). For L.a, the largest inhibition halo was observed for the Chx, followed by EO at 1%, 0.2%, 0.135% and Ac. For evaluation of cytotoxicity, the following groups were set: G1: 0,1% EO; G2: pure EO; G3 (positive control): H2 O2 ; G4: cereal alcohol; and G5 (negative control): culture medium – DMEM. The solutions were applied on the cultured MDPC-23 cells, which were plated (30,000 cells/cm2 ) in wells of 24 well-dishes. Cell metabolism was evaluated by MTT assay. Considering G5 (negative control) as 100% of cell metabolism, it was observed for G1, G2, G3 and G4 a percentage reduction in cell metabolism of 29.6%, 82%, 81.2% and 33.4%, respectively. It was concluded that the low concentration of 0,1% OE (C. citratus) was able to inhibit the growth of the strains tested as well as caused mild cytotoxicity to the cultured MDPC-23 cells. |
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Efeito antimicrobiano e citotóxico do óleo essencial de Cymbopogon citratus sobre células odontoblastóidesAntimicrobial and cytotoxic effects of the essential oil of Cymbopogon citratus on odontoblast-like cellsCytotoxicityAntimicrobial activityEssential oilMedicinal plantCitotoxicidadeAtividade antimicrobianaÓleo essencialPlantas medicinaisThe aim of this study was to evaluate the antimicrobial and cytotoxic effect of essential oil (EO) of lemon grass (Cymbopogon citratus). From the agar diffusion method, different concentrations of EO (0.135%, 0.2% and 1%), and control solutions (chlorhexidine (Chx), distilled water (Ad) and cereal alcohol (Ac)) were applied on cultures of Candida albicans (C.a), Streptococcus mutans (S.m), Streptococcus sobrinus (S.sob) and Lactobacillus acidophilus (L.a). For C.a, S.m and S.sob, the largest inhibition zones in descending order were: Chx, Ac and EO 1%, while the latter two were statistically similar (Mann-Whitney, p> 0.05). For L.a, the largest inhibition halo was observed for the Chx, followed by EO at 1%, 0.2%, 0.135% and Ac. For evaluation of cytotoxicity, the following groups were set: G1: 0,1% EO; G2: pure EO; G3 (positive control): H2 O2 ; G4: cereal alcohol; and G5 (negative control): culture medium – DMEM. The solutions were applied on the cultured MDPC-23 cells, which were plated (30,000 cells/cm2 ) in wells of 24 well-dishes. Cell metabolism was evaluated by MTT assay. Considering G5 (negative control) as 100% of cell metabolism, it was observed for G1, G2, G3 and G4 a percentage reduction in cell metabolism of 29.6%, 82%, 81.2% and 33.4%, respectively. It was concluded that the low concentration of 0,1% OE (C. citratus) was able to inhibit the growth of the strains tested as well as caused mild cytotoxicity to the cultured MDPC-23 cells.O objetivo deste trabalho foi avaliar a atividade antimicrobiana e o efeito citotóxico do óleo essencial (OE) de capim-limão (Cymbopogon citratus). A partir do método de difusão em ágar, diferentes concentrações de OE (0,1%; 0,2% e 1%), e soluções controle (clorexidina (Chx), água destilada (Ad) e álcool de cereais (Ac) foram aplicados sobre culturas de Candida albicans (C.a), Streptococos mutans (S.m), Streptococos sobrinus (S.sob) e Lactobacilus acidofilus (L.a). Para C.a, S.m e S.sob, os maiores halos de inibição, em ordem decrescente foram: Chx, Ac e óleo 1%, sendo os dois últimos semelhantes estatisticamente (Mann-Whitney, p>0,05). Para L.a, o maior halo de inibição foi observado para a Chx, seguido do óleo a 1%, 0,2%, 0,1% e Ac. Para avaliação da citotoxicidade foram determinados os seguintes grupos: OE a 0,1%; G2: OE puro; G3 (controle positivo): H2 O2 ; G4: álcool de cereais (Ac); e G5 (controle negativo): meio de cultura (DMEM). As soluções foram aplicadas sobre cultura de células MDPC-23 (30.000 células/cm2 ) semeadas em placas de 24 wells. O metabolismo celular foi avaliado pelo teste do MTT. Considerando G5 como 100% de metabolismo celular, foi observado para os grupos G1, G2, G3, e G4 uma redução percentual no metabolismo das células de 29,6%; 82%; 81,2%; e 33,4%, respectivamente. Concluiu-se que o OE a 0,1% foi capaz de inibir o crescimento das cepas avaliadas e de causar discreta citotoxicidade sobre cé- lulas odontoblastóides MDPC-23.Universidade Estadual Paulista Júlio de Mesquita Filho, Departamento de Clínica Infantil, Faculdade de Odontologia de Araraquara, Araraquara, Rua Humaitá, 1680, Centro, CEP 14801-903, SP, BrasilUniversidade Estadual Paulista Júlio de Mesquita Filho, Departamento de Clínica Infantil, Faculdade de Odontologia de Araraquara, Araraquara, Rua Humaitá, 1680, Centro, CEP 14801-903, SP, BrasilDepartamento de Princípios Ativos Naturais e Toxicologia da Faculdade de Ciências Farmacêuticas de AraraquaraDepartamento de Fisiologia e Patologia da Faculdade de Odontologia de AraraquaraUniversidade Estadual Paulista (Unesp)Vargas, Fernanda S. [UNESP]Oliveira, Camila Fávero de [UNESP]Giro, Elisa Maria Aparecida [UNESP]Sacramento, Luis V. S. [UNESP]Spolidório, Denise Madalena Palomari [UNESP]Costa, Carlos Alberto de Souza [UNESP]2015-08-06T16:12:33Z2015-08-06T16:12:33Z2010info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article101-107application/pdfhttp://www.robrac.org.br/seer/index.php/ROBRAC/article/view/438ROBRAC, v. 19, n. 49, p. 101-107, 2010.1981-3708http://hdl.handle.net/11449/125591ISSN1981-3708-2010-19-49-101-107.pdf2948162075170172009703168206365237069310615345324517484241515548Currículo Lattesreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPporROBRACinfo:eu-repo/semantics/openAccess2024-06-24T14:52:03Zoai:repositorio.unesp.br:11449/125591Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-06T00:01:49.764169Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Efeito antimicrobiano e citotóxico do óleo essencial de Cymbopogon citratus sobre células odontoblastóides Antimicrobial and cytotoxic effects of the essential oil of Cymbopogon citratus on odontoblast-like cells |
title |
Efeito antimicrobiano e citotóxico do óleo essencial de Cymbopogon citratus sobre células odontoblastóides |
spellingShingle |
Efeito antimicrobiano e citotóxico do óleo essencial de Cymbopogon citratus sobre células odontoblastóides Vargas, Fernanda S. [UNESP] Cytotoxicity Antimicrobial activity Essential oil Medicinal plant Citotoxicidade Atividade antimicrobiana Óleo essencial Plantas medicinais |
title_short |
Efeito antimicrobiano e citotóxico do óleo essencial de Cymbopogon citratus sobre células odontoblastóides |
title_full |
Efeito antimicrobiano e citotóxico do óleo essencial de Cymbopogon citratus sobre células odontoblastóides |
title_fullStr |
Efeito antimicrobiano e citotóxico do óleo essencial de Cymbopogon citratus sobre células odontoblastóides |
title_full_unstemmed |
Efeito antimicrobiano e citotóxico do óleo essencial de Cymbopogon citratus sobre células odontoblastóides |
title_sort |
Efeito antimicrobiano e citotóxico do óleo essencial de Cymbopogon citratus sobre células odontoblastóides |
author |
Vargas, Fernanda S. [UNESP] |
author_facet |
Vargas, Fernanda S. [UNESP] Oliveira, Camila Fávero de [UNESP] Giro, Elisa Maria Aparecida [UNESP] Sacramento, Luis V. S. [UNESP] Spolidório, Denise Madalena Palomari [UNESP] Costa, Carlos Alberto de Souza [UNESP] |
author_role |
author |
author2 |
Oliveira, Camila Fávero de [UNESP] Giro, Elisa Maria Aparecida [UNESP] Sacramento, Luis V. S. [UNESP] Spolidório, Denise Madalena Palomari [UNESP] Costa, Carlos Alberto de Souza [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Vargas, Fernanda S. [UNESP] Oliveira, Camila Fávero de [UNESP] Giro, Elisa Maria Aparecida [UNESP] Sacramento, Luis V. S. [UNESP] Spolidório, Denise Madalena Palomari [UNESP] Costa, Carlos Alberto de Souza [UNESP] |
dc.subject.por.fl_str_mv |
Cytotoxicity Antimicrobial activity Essential oil Medicinal plant Citotoxicidade Atividade antimicrobiana Óleo essencial Plantas medicinais |
topic |
Cytotoxicity Antimicrobial activity Essential oil Medicinal plant Citotoxicidade Atividade antimicrobiana Óleo essencial Plantas medicinais |
description |
The aim of this study was to evaluate the antimicrobial and cytotoxic effect of essential oil (EO) of lemon grass (Cymbopogon citratus). From the agar diffusion method, different concentrations of EO (0.135%, 0.2% and 1%), and control solutions (chlorhexidine (Chx), distilled water (Ad) and cereal alcohol (Ac)) were applied on cultures of Candida albicans (C.a), Streptococcus mutans (S.m), Streptococcus sobrinus (S.sob) and Lactobacillus acidophilus (L.a). For C.a, S.m and S.sob, the largest inhibition zones in descending order were: Chx, Ac and EO 1%, while the latter two were statistically similar (Mann-Whitney, p> 0.05). For L.a, the largest inhibition halo was observed for the Chx, followed by EO at 1%, 0.2%, 0.135% and Ac. For evaluation of cytotoxicity, the following groups were set: G1: 0,1% EO; G2: pure EO; G3 (positive control): H2 O2 ; G4: cereal alcohol; and G5 (negative control): culture medium – DMEM. The solutions were applied on the cultured MDPC-23 cells, which were plated (30,000 cells/cm2 ) in wells of 24 well-dishes. Cell metabolism was evaluated by MTT assay. Considering G5 (negative control) as 100% of cell metabolism, it was observed for G1, G2, G3 and G4 a percentage reduction in cell metabolism of 29.6%, 82%, 81.2% and 33.4%, respectively. It was concluded that the low concentration of 0,1% OE (C. citratus) was able to inhibit the growth of the strains tested as well as caused mild cytotoxicity to the cultured MDPC-23 cells. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010 2015-08-06T16:12:33Z 2015-08-06T16:12:33Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.robrac.org.br/seer/index.php/ROBRAC/article/view/438 ROBRAC, v. 19, n. 49, p. 101-107, 2010. 1981-3708 http://hdl.handle.net/11449/125591 ISSN1981-3708-2010-19-49-101-107.pdf 2948162075170172 0097031682063652 3706931061534532 4517484241515548 |
url |
http://www.robrac.org.br/seer/index.php/ROBRAC/article/view/438 http://hdl.handle.net/11449/125591 |
identifier_str_mv |
ROBRAC, v. 19, n. 49, p. 101-107, 2010. 1981-3708 ISSN1981-3708-2010-19-49-101-107.pdf 2948162075170172 0097031682063652 3706931061534532 4517484241515548 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
ROBRAC |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
101-107 application/pdf |
dc.source.none.fl_str_mv |
Currículo Lattes reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808129574321520640 |