Effects of long-term fluoride exposure are associated with oxidative biochemistry impairment and global proteomic modulation, but not genotoxicity, in parotid glands of mice

Detalhes bibliográficos
Autor(a) principal: Miranda, Giza Hellen Nonato
Data de Publicação: 2022
Outros Autores: De Oliveira Lima, Leidiane Alencar, Bittencourt, Leonardo Oliveira, Dos Santos, Savio Monteiro, De Souza, Michel Platini Caldas, Nogueira, Lygia Sega, De Oliveira, Edivaldo Herculano Corrêa, Monteiro, Marta Chagas, Dionizio, Aline, Leite, Aline Lima, Pessan, Juliano Pelim [UNESP], Buzalaf, Marilia Afonso Rabelo, Lima, Rafael Rodrigues
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1371/journal.pone.0261252
http://hdl.handle.net/11449/231610
Resumo: Background Fluoride has become widely used in dentistry because of its effectiveness in caries control. However, evidence indicates that excessive intake interferes with the metabolic processes of different tissues. Thus, this study aimed to investigate the effects of long-term exposure to F on the parotid salivary gland of mice, from the analysis of oxidative, proteomic and genotoxic parameters. Materials and methods The animals received deionized water containing 0, 10 or 50 mg/L of F, as sodium fluoride, for 60 days. After, parotid glands were collected for analysis of oxidative biochemistry, global proteomic profile, genotoxicity assessment and histopathological analyses. Results The results revealed that exposure to fluoride interfered in the biochemical homeostasis of the parotid gland, with increased levels of thiobarbituric acid reactive species and reduced glutathione in the exposed groups; as well as promoted alteration of the glandular proteomic profile in these groups, especially in structural proteins and proteins related to oxidative stress. However, genotoxic assessment demonstrated that exposure to fluoride did not interfere with DNA integrity in these concentrations and durations of exposure. Also, it was not observed histopathological alterations in parotid gland. Conclusions Thus, our results suggest that long-term exposure to fluoride promoted modulation of the proteomic and biochemical profile in the parotid glands, without inducing damage to the genetic component. These findings reinforce the importance of rationalizing the use of fluorides to maximize their preventative effects while minimizing the environmental risks.
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spelling Effects of long-term fluoride exposure are associated with oxidative biochemistry impairment and global proteomic modulation, but not genotoxicity, in parotid glands of miceBackground Fluoride has become widely used in dentistry because of its effectiveness in caries control. However, evidence indicates that excessive intake interferes with the metabolic processes of different tissues. Thus, this study aimed to investigate the effects of long-term exposure to F on the parotid salivary gland of mice, from the analysis of oxidative, proteomic and genotoxic parameters. Materials and methods The animals received deionized water containing 0, 10 or 50 mg/L of F, as sodium fluoride, for 60 days. After, parotid glands were collected for analysis of oxidative biochemistry, global proteomic profile, genotoxicity assessment and histopathological analyses. Results The results revealed that exposure to fluoride interfered in the biochemical homeostasis of the parotid gland, with increased levels of thiobarbituric acid reactive species and reduced glutathione in the exposed groups; as well as promoted alteration of the glandular proteomic profile in these groups, especially in structural proteins and proteins related to oxidative stress. However, genotoxic assessment demonstrated that exposure to fluoride did not interfere with DNA integrity in these concentrations and durations of exposure. Also, it was not observed histopathological alterations in parotid gland. Conclusions Thus, our results suggest that long-term exposure to fluoride promoted modulation of the proteomic and biochemical profile in the parotid glands, without inducing damage to the genetic component. These findings reinforce the importance of rationalizing the use of fluorides to maximize their preventative effects while minimizing the environmental risks.Laboratory of Functional and Structural Biology Institute of Biological Sciences Federal University of Para, PALaboratory of Clinical Immunology and Oxidative Stress Pharmacy Faculty Institute of Health Science Federal University of Para, PAEvandro Chagas Institute, PADepartment of Biological Sciences Bauru Dental School University of São Paulo, SPDepartment of Chemistry University of Nebraska-LincolnDepartment of Preventive and Restorative Dentistry School of Dentistry São Paulo State University (UNESP), SPDepartment of Preventive and Restorative Dentistry School of Dentistry São Paulo State University (UNESP), SPFederal University of ParaEvandro Chagas InstituteUniversidade de São Paulo (USP)University of Nebraska-LincolnUniversidade Estadual Paulista (UNESP)Miranda, Giza Hellen NonatoDe Oliveira Lima, Leidiane AlencarBittencourt, Leonardo OliveiraDos Santos, Savio MonteiroDe Souza, Michel Platini CaldasNogueira, Lygia SegaDe Oliveira, Edivaldo Herculano CorrêaMonteiro, Marta ChagasDionizio, AlineLeite, Aline LimaPessan, Juliano Pelim [UNESP]Buzalaf, Marilia Afonso RabeloLima, Rafael Rodrigues2022-04-29T08:46:34Z2022-04-29T08:46:34Z2022-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1371/journal.pone.0261252PLoS ONE, v. 17, n. 1 January, 2022.1932-6203http://hdl.handle.net/11449/23161010.1371/journal.pone.02612522-s2.0-85123743047Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPLoS ONEinfo:eu-repo/semantics/openAccess2024-04-23T15:23:39Zoai:repositorio.unesp.br:11449/231610Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:12:30.389579Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Effects of long-term fluoride exposure are associated with oxidative biochemistry impairment and global proteomic modulation, but not genotoxicity, in parotid glands of mice
title Effects of long-term fluoride exposure are associated with oxidative biochemistry impairment and global proteomic modulation, but not genotoxicity, in parotid glands of mice
spellingShingle Effects of long-term fluoride exposure are associated with oxidative biochemistry impairment and global proteomic modulation, but not genotoxicity, in parotid glands of mice
Miranda, Giza Hellen Nonato
title_short Effects of long-term fluoride exposure are associated with oxidative biochemistry impairment and global proteomic modulation, but not genotoxicity, in parotid glands of mice
title_full Effects of long-term fluoride exposure are associated with oxidative biochemistry impairment and global proteomic modulation, but not genotoxicity, in parotid glands of mice
title_fullStr Effects of long-term fluoride exposure are associated with oxidative biochemistry impairment and global proteomic modulation, but not genotoxicity, in parotid glands of mice
title_full_unstemmed Effects of long-term fluoride exposure are associated with oxidative biochemistry impairment and global proteomic modulation, but not genotoxicity, in parotid glands of mice
title_sort Effects of long-term fluoride exposure are associated with oxidative biochemistry impairment and global proteomic modulation, but not genotoxicity, in parotid glands of mice
author Miranda, Giza Hellen Nonato
author_facet Miranda, Giza Hellen Nonato
De Oliveira Lima, Leidiane Alencar
Bittencourt, Leonardo Oliveira
Dos Santos, Savio Monteiro
De Souza, Michel Platini Caldas
Nogueira, Lygia Sega
De Oliveira, Edivaldo Herculano Corrêa
Monteiro, Marta Chagas
Dionizio, Aline
Leite, Aline Lima
Pessan, Juliano Pelim [UNESP]
Buzalaf, Marilia Afonso Rabelo
Lima, Rafael Rodrigues
author_role author
author2 De Oliveira Lima, Leidiane Alencar
Bittencourt, Leonardo Oliveira
Dos Santos, Savio Monteiro
De Souza, Michel Platini Caldas
Nogueira, Lygia Sega
De Oliveira, Edivaldo Herculano Corrêa
Monteiro, Marta Chagas
Dionizio, Aline
Leite, Aline Lima
Pessan, Juliano Pelim [UNESP]
Buzalaf, Marilia Afonso Rabelo
Lima, Rafael Rodrigues
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Federal University of Para
Evandro Chagas Institute
Universidade de São Paulo (USP)
University of Nebraska-Lincoln
Universidade Estadual Paulista (UNESP)
dc.contributor.author.fl_str_mv Miranda, Giza Hellen Nonato
De Oliveira Lima, Leidiane Alencar
Bittencourt, Leonardo Oliveira
Dos Santos, Savio Monteiro
De Souza, Michel Platini Caldas
Nogueira, Lygia Sega
De Oliveira, Edivaldo Herculano Corrêa
Monteiro, Marta Chagas
Dionizio, Aline
Leite, Aline Lima
Pessan, Juliano Pelim [UNESP]
Buzalaf, Marilia Afonso Rabelo
Lima, Rafael Rodrigues
description Background Fluoride has become widely used in dentistry because of its effectiveness in caries control. However, evidence indicates that excessive intake interferes with the metabolic processes of different tissues. Thus, this study aimed to investigate the effects of long-term exposure to F on the parotid salivary gland of mice, from the analysis of oxidative, proteomic and genotoxic parameters. Materials and methods The animals received deionized water containing 0, 10 or 50 mg/L of F, as sodium fluoride, for 60 days. After, parotid glands were collected for analysis of oxidative biochemistry, global proteomic profile, genotoxicity assessment and histopathological analyses. Results The results revealed that exposure to fluoride interfered in the biochemical homeostasis of the parotid gland, with increased levels of thiobarbituric acid reactive species and reduced glutathione in the exposed groups; as well as promoted alteration of the glandular proteomic profile in these groups, especially in structural proteins and proteins related to oxidative stress. However, genotoxic assessment demonstrated that exposure to fluoride did not interfere with DNA integrity in these concentrations and durations of exposure. Also, it was not observed histopathological alterations in parotid gland. Conclusions Thus, our results suggest that long-term exposure to fluoride promoted modulation of the proteomic and biochemical profile in the parotid glands, without inducing damage to the genetic component. These findings reinforce the importance of rationalizing the use of fluorides to maximize their preventative effects while minimizing the environmental risks.
publishDate 2022
dc.date.none.fl_str_mv 2022-04-29T08:46:34Z
2022-04-29T08:46:34Z
2022-01-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1371/journal.pone.0261252
PLoS ONE, v. 17, n. 1 January, 2022.
1932-6203
http://hdl.handle.net/11449/231610
10.1371/journal.pone.0261252
2-s2.0-85123743047
url http://dx.doi.org/10.1371/journal.pone.0261252
http://hdl.handle.net/11449/231610
identifier_str_mv PLoS ONE, v. 17, n. 1 January, 2022.
1932-6203
10.1371/journal.pone.0261252
2-s2.0-85123743047
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv PLoS ONE
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repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
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