Effect of follicular fluid supplementation during in vitro maturation on total cell number in bovine blastocysts produced in vitro
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S1516-35982014000300003 http://hdl.handle.net/11449/113075 |
Resumo: | This study evaluated the influence of follicular fluid (FF) added to the maturation medium on the quality of bovine embryos produced in vitro. In the first experiment, oocytes were matured in media containing five different FF concentrations with different maturation times and classified according to meiotic progression and migration of cortical granules. In the second experiment, oocytes matured in the same media were fertilized at three different maturation times; thereafter, cleavage and blastocyst rates were evaluated. In the third experiment, oocytes were matured in media containing three different FF concentrations at two different maturation times, and embryo quality, inferred by the ratio of inner cell mass and trophectoderm cells compared with total cell number, was evaluated. Higher FF concentration (75 - 100% FF) slowed meiotic progression and CG migration (control - 78.13% vs. treated - 52.58% and control - 52.7% vs. treated - 11.59%, respectively, at 24 h of maturation). Also, FF at concentration of 75% or 100% had a negative influence on cleavage and blastocyst rates (control - 90.13% vs. treated - 82.64% and control - 35.73% vs. treated - 11.57%, respectively, at 24 h of maturation). The 50% FF resulted in embryos with increased inner cell mass numbers (control - 29.91 vs. treated - 35.49, at 24 h of maturation) and total cell numbers (control - 109.53 vs. treated - 120.67, at 26 h of maturation). Even though higher concentration of FF added to the maturation medium reduced embryonic development rates, in lower concentrations, FF slowed the meiotic progression and migration of CG and contributed to increases in inner cell mass number. Thus, FF added to the maturation medium enhances the number of cells in bovine embryos produced in vitro, especially for inner cell mass. |
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Repositório Institucional da UNESP |
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2946 |
spelling |
Effect of follicular fluid supplementation during in vitro maturation on total cell number in bovine blastocysts produced in vitroembryo developmentinner cell massIn vitro fertilizationOocytesThis study evaluated the influence of follicular fluid (FF) added to the maturation medium on the quality of bovine embryos produced in vitro. In the first experiment, oocytes were matured in media containing five different FF concentrations with different maturation times and classified according to meiotic progression and migration of cortical granules. In the second experiment, oocytes matured in the same media were fertilized at three different maturation times; thereafter, cleavage and blastocyst rates were evaluated. In the third experiment, oocytes were matured in media containing three different FF concentrations at two different maturation times, and embryo quality, inferred by the ratio of inner cell mass and trophectoderm cells compared with total cell number, was evaluated. Higher FF concentration (75 - 100% FF) slowed meiotic progression and CG migration (control - 78.13% vs. treated - 52.58% and control - 52.7% vs. treated - 11.59%, respectively, at 24 h of maturation). Also, FF at concentration of 75% or 100% had a negative influence on cleavage and blastocyst rates (control - 90.13% vs. treated - 82.64% and control - 35.73% vs. treated - 11.57%, respectively, at 24 h of maturation). The 50% FF resulted in embryos with increased inner cell mass numbers (control - 29.91 vs. treated - 35.49, at 24 h of maturation) and total cell numbers (control - 109.53 vs. treated - 120.67, at 26 h of maturation). Even though higher concentration of FF added to the maturation medium reduced embryonic development rates, in lower concentrations, FF slowed the meiotic progression and migration of CG and contributed to increases in inner cell mass number. Thus, FF added to the maturation medium enhances the number of cells in bovine embryos produced in vitro, especially for inner cell mass.Univ Estadual Paulista, Dept Med Vet Prevent & Reprod Anim, Jaboticabal, SP, BrazilUniv Estadual Sudoeste Bahia, Lab Reprod Caprinos & Ovinos, Vitoria Da Conquista, BA, BrazilUniv Sao Paulo, Dept Ciencias Bas, Pirassununga, SP, BrazilUniv Estadual Paulista, Dept Med Vet Prevent & Reprod Anim, Jaboticabal, SP, BrazilRevista Brasileira Zootecnia Brazilian Journal Animal SciUniversidade Estadual Paulista (Unesp)Univ Estadual Sudoeste BahiaUniversidade de São Paulo (USP)Coelho Cruz, Maria Helena [UNESP]Saraiva, Naiara Zoccal [UNESP]Cruz, Jurandir Ferreira daOliveira, Clara Slade [UNESP]Del Collado, Maite [UNESP]Fernandes, HugoCastro, Fernanda Cavallari deGarcia, Joaquim Mansano [UNESP]2014-12-03T13:11:24Z2014-12-03T13:11:24Z2014-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article120-126application/pdfhttp://dx.doi.org/10.1590/S1516-35982014000300003Revista Brasileira de Zootecnia-brazilian Journal of Animal Science. Vicosa-mg: Revista Brasileira Zootecnia Brazilian Journal Animal Sci, v. 43, n. 3, p. 120-126, 2014.1806-9290http://hdl.handle.net/11449/113075S1516-35982014000300003WOS:000332924000003S1516-35982014000300003.pdf2251116139872527Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengRevista Brasileira de Zootecnia = Brazilian Journal of Animal Science0.735info:eu-repo/semantics/openAccess2024-06-06T18:09:21Zoai:repositorio.unesp.br:11449/113075Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T16:28:26.559337Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Effect of follicular fluid supplementation during in vitro maturation on total cell number in bovine blastocysts produced in vitro |
title |
Effect of follicular fluid supplementation during in vitro maturation on total cell number in bovine blastocysts produced in vitro |
spellingShingle |
Effect of follicular fluid supplementation during in vitro maturation on total cell number in bovine blastocysts produced in vitro Coelho Cruz, Maria Helena [UNESP] embryo development inner cell mass In vitro fertilization Oocytes |
title_short |
Effect of follicular fluid supplementation during in vitro maturation on total cell number in bovine blastocysts produced in vitro |
title_full |
Effect of follicular fluid supplementation during in vitro maturation on total cell number in bovine blastocysts produced in vitro |
title_fullStr |
Effect of follicular fluid supplementation during in vitro maturation on total cell number in bovine blastocysts produced in vitro |
title_full_unstemmed |
Effect of follicular fluid supplementation during in vitro maturation on total cell number in bovine blastocysts produced in vitro |
title_sort |
Effect of follicular fluid supplementation during in vitro maturation on total cell number in bovine blastocysts produced in vitro |
author |
Coelho Cruz, Maria Helena [UNESP] |
author_facet |
Coelho Cruz, Maria Helena [UNESP] Saraiva, Naiara Zoccal [UNESP] Cruz, Jurandir Ferreira da Oliveira, Clara Slade [UNESP] Del Collado, Maite [UNESP] Fernandes, Hugo Castro, Fernanda Cavallari de Garcia, Joaquim Mansano [UNESP] |
author_role |
author |
author2 |
Saraiva, Naiara Zoccal [UNESP] Cruz, Jurandir Ferreira da Oliveira, Clara Slade [UNESP] Del Collado, Maite [UNESP] Fernandes, Hugo Castro, Fernanda Cavallari de Garcia, Joaquim Mansano [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Univ Estadual Sudoeste Bahia Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Coelho Cruz, Maria Helena [UNESP] Saraiva, Naiara Zoccal [UNESP] Cruz, Jurandir Ferreira da Oliveira, Clara Slade [UNESP] Del Collado, Maite [UNESP] Fernandes, Hugo Castro, Fernanda Cavallari de Garcia, Joaquim Mansano [UNESP] |
dc.subject.por.fl_str_mv |
embryo development inner cell mass In vitro fertilization Oocytes |
topic |
embryo development inner cell mass In vitro fertilization Oocytes |
description |
This study evaluated the influence of follicular fluid (FF) added to the maturation medium on the quality of bovine embryos produced in vitro. In the first experiment, oocytes were matured in media containing five different FF concentrations with different maturation times and classified according to meiotic progression and migration of cortical granules. In the second experiment, oocytes matured in the same media were fertilized at three different maturation times; thereafter, cleavage and blastocyst rates were evaluated. In the third experiment, oocytes were matured in media containing three different FF concentrations at two different maturation times, and embryo quality, inferred by the ratio of inner cell mass and trophectoderm cells compared with total cell number, was evaluated. Higher FF concentration (75 - 100% FF) slowed meiotic progression and CG migration (control - 78.13% vs. treated - 52.58% and control - 52.7% vs. treated - 11.59%, respectively, at 24 h of maturation). Also, FF at concentration of 75% or 100% had a negative influence on cleavage and blastocyst rates (control - 90.13% vs. treated - 82.64% and control - 35.73% vs. treated - 11.57%, respectively, at 24 h of maturation). The 50% FF resulted in embryos with increased inner cell mass numbers (control - 29.91 vs. treated - 35.49, at 24 h of maturation) and total cell numbers (control - 109.53 vs. treated - 120.67, at 26 h of maturation). Even though higher concentration of FF added to the maturation medium reduced embryonic development rates, in lower concentrations, FF slowed the meiotic progression and migration of CG and contributed to increases in inner cell mass number. Thus, FF added to the maturation medium enhances the number of cells in bovine embryos produced in vitro, especially for inner cell mass. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-12-03T13:11:24Z 2014-12-03T13:11:24Z 2014-03-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S1516-35982014000300003 Revista Brasileira de Zootecnia-brazilian Journal of Animal Science. Vicosa-mg: Revista Brasileira Zootecnia Brazilian Journal Animal Sci, v. 43, n. 3, p. 120-126, 2014. 1806-9290 http://hdl.handle.net/11449/113075 S1516-35982014000300003 WOS:000332924000003 S1516-35982014000300003.pdf 2251116139872527 |
url |
http://dx.doi.org/10.1590/S1516-35982014000300003 http://hdl.handle.net/11449/113075 |
identifier_str_mv |
Revista Brasileira de Zootecnia-brazilian Journal of Animal Science. Vicosa-mg: Revista Brasileira Zootecnia Brazilian Journal Animal Sci, v. 43, n. 3, p. 120-126, 2014. 1806-9290 S1516-35982014000300003 WOS:000332924000003 S1516-35982014000300003.pdf 2251116139872527 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Revista Brasileira de Zootecnia = Brazilian Journal of Animal Science 0.735 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
120-126 application/pdf |
dc.publisher.none.fl_str_mv |
Revista Brasileira Zootecnia Brazilian Journal Animal Sci |
publisher.none.fl_str_mv |
Revista Brasileira Zootecnia Brazilian Journal Animal Sci |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128657668964352 |