In vitro effect of triamcinolone and platelet-rich plasma on cytokine levels of elbow lateral epicondylitis-derived cells

Detalhes bibliográficos
Autor(a) principal: de Melo Viveiros, Márcio Eduardo [UNESP]
Data de Publicação: 2022
Outros Autores: Viveiros, Magda Massae Hata [UNESP], da Silva, Márcia Guimarães [UNESP], Rainho, Cláudia Aparecida [UNESP], Schellini, Silvana Artioli [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1186/s13018-022-02990-0
http://hdl.handle.net/11449/230393
Resumo: Background: The pathogenesis and treatment of lateral elbow epicondylitis (LEE) are still controversial. The purpose of the current study was to evaluate the production of inflammatory cytokines by LEE-derived cells and to compare the anti-inflammatory effect of triamcinolone acetonide with platelet-rich plasma (PRP) on cytokines production in primary culture of these cells. Methods: Third passage cells from primary cultures of LEE were assessed for the production of the cytokines IL-1β, IL-6, IL-8, IL-10 and TNF-α by immune-enzymatic assay (ELISA), after the treatment with 1, 10 and 100 μM triamcinolone compared to no treated controls at the time points 6, 12, 18, 24, 48, 72 and 96 h, and to PRP at 48, 72 and 96 h. Results: The cytokines IL-6 and IL-8 were produced in high concentrations by LEE cells. One, 10 and 100 μM triamcinolone induced significant decrease in the production of IL-6 and IL-8 at 48, 72 and 96 h, adding the time point 12 h for IL-8. Compared to controls, PRP caused a significant increase in the production of IL-6 and IL-8 and there was a significant increase in IL-10 production with the use of 100 μM triamcinolone at 48 h. The production of IL1-β and TNF-α was very low and did not change when the cultures were treated with triamcinolone or PRP. Conclusion: LEE-derived cells produce IL-6 and IL-8, confirming the inflammatory nature of this condition. While triamcinolone inhibited the production of IL-6 and IL-8 by LEE cells, PRP induced an increase in these cytokines compared with controls.
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spelling In vitro effect of triamcinolone and platelet-rich plasma on cytokine levels of elbow lateral epicondylitis-derived cellsCytokinesInflammationLateral elbow epicondylitisPRPTriamcinoloneBackground: The pathogenesis and treatment of lateral elbow epicondylitis (LEE) are still controversial. The purpose of the current study was to evaluate the production of inflammatory cytokines by LEE-derived cells and to compare the anti-inflammatory effect of triamcinolone acetonide with platelet-rich plasma (PRP) on cytokines production in primary culture of these cells. Methods: Third passage cells from primary cultures of LEE were assessed for the production of the cytokines IL-1β, IL-6, IL-8, IL-10 and TNF-α by immune-enzymatic assay (ELISA), after the treatment with 1, 10 and 100 μM triamcinolone compared to no treated controls at the time points 6, 12, 18, 24, 48, 72 and 96 h, and to PRP at 48, 72 and 96 h. Results: The cytokines IL-6 and IL-8 were produced in high concentrations by LEE cells. One, 10 and 100 μM triamcinolone induced significant decrease in the production of IL-6 and IL-8 at 48, 72 and 96 h, adding the time point 12 h for IL-8. Compared to controls, PRP caused a significant increase in the production of IL-6 and IL-8 and there was a significant increase in IL-10 production with the use of 100 μM triamcinolone at 48 h. The production of IL1-β and TNF-α was very low and did not change when the cultures were treated with triamcinolone or PRP. Conclusion: LEE-derived cells produce IL-6 and IL-8, confirming the inflammatory nature of this condition. While triamcinolone inhibited the production of IL-6 and IL-8 by LEE cells, PRP induced an increase in these cytokines compared with controls.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Orthopedics and Traumatology School of Medicine of Botucatu São Paulo State University – UNESP, SPDepartment of Ophthalmology School of Medicine of Botucatu São Paulo State University – UNESP, SPDepartment of Pathology School of Medicine of Botucatu São Paulo State University – UNESP, SPDepartment of Chemical and Biological Sciences Institute of Biosciences of Botucatu São Paulo State University – UNESP, SPDepartment of Orthopedics and Traumatology School of Medicine of Botucatu São Paulo State University – UNESP, SPDepartment of Ophthalmology School of Medicine of Botucatu São Paulo State University – UNESP, SPDepartment of Pathology School of Medicine of Botucatu São Paulo State University – UNESP, SPDepartment of Chemical and Biological Sciences Institute of Biosciences of Botucatu São Paulo State University – UNESP, SPFAPESP: 2019/13468-7Universidade Estadual Paulista (UNESP)de Melo Viveiros, Márcio Eduardo [UNESP]Viveiros, Magda Massae Hata [UNESP]da Silva, Márcia Guimarães [UNESP]Rainho, Cláudia Aparecida [UNESP]Schellini, Silvana Artioli [UNESP]2022-04-29T08:39:35Z2022-04-29T08:39:35Z2022-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1186/s13018-022-02990-0Journal of Orthopaedic Surgery and Research, v. 17, n. 1, 2022.1749-799Xhttp://hdl.handle.net/11449/23039310.1186/s13018-022-02990-02-s2.0-85124680382Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Orthopaedic Surgery and Researchinfo:eu-repo/semantics/openAccess2024-09-03T13:18:32Zoai:repositorio.unesp.br:11449/230393Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-03T13:18:32Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv In vitro effect of triamcinolone and platelet-rich plasma on cytokine levels of elbow lateral epicondylitis-derived cells
title In vitro effect of triamcinolone and platelet-rich plasma on cytokine levels of elbow lateral epicondylitis-derived cells
spellingShingle In vitro effect of triamcinolone and platelet-rich plasma on cytokine levels of elbow lateral epicondylitis-derived cells
de Melo Viveiros, Márcio Eduardo [UNESP]
Cytokines
Inflammation
Lateral elbow epicondylitis
PRP
Triamcinolone
title_short In vitro effect of triamcinolone and platelet-rich plasma on cytokine levels of elbow lateral epicondylitis-derived cells
title_full In vitro effect of triamcinolone and platelet-rich plasma on cytokine levels of elbow lateral epicondylitis-derived cells
title_fullStr In vitro effect of triamcinolone and platelet-rich plasma on cytokine levels of elbow lateral epicondylitis-derived cells
title_full_unstemmed In vitro effect of triamcinolone and platelet-rich plasma on cytokine levels of elbow lateral epicondylitis-derived cells
title_sort In vitro effect of triamcinolone and platelet-rich plasma on cytokine levels of elbow lateral epicondylitis-derived cells
author de Melo Viveiros, Márcio Eduardo [UNESP]
author_facet de Melo Viveiros, Márcio Eduardo [UNESP]
Viveiros, Magda Massae Hata [UNESP]
da Silva, Márcia Guimarães [UNESP]
Rainho, Cláudia Aparecida [UNESP]
Schellini, Silvana Artioli [UNESP]
author_role author
author2 Viveiros, Magda Massae Hata [UNESP]
da Silva, Márcia Guimarães [UNESP]
Rainho, Cláudia Aparecida [UNESP]
Schellini, Silvana Artioli [UNESP]
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
dc.contributor.author.fl_str_mv de Melo Viveiros, Márcio Eduardo [UNESP]
Viveiros, Magda Massae Hata [UNESP]
da Silva, Márcia Guimarães [UNESP]
Rainho, Cláudia Aparecida [UNESP]
Schellini, Silvana Artioli [UNESP]
dc.subject.por.fl_str_mv Cytokines
Inflammation
Lateral elbow epicondylitis
PRP
Triamcinolone
topic Cytokines
Inflammation
Lateral elbow epicondylitis
PRP
Triamcinolone
description Background: The pathogenesis and treatment of lateral elbow epicondylitis (LEE) are still controversial. The purpose of the current study was to evaluate the production of inflammatory cytokines by LEE-derived cells and to compare the anti-inflammatory effect of triamcinolone acetonide with platelet-rich plasma (PRP) on cytokines production in primary culture of these cells. Methods: Third passage cells from primary cultures of LEE were assessed for the production of the cytokines IL-1β, IL-6, IL-8, IL-10 and TNF-α by immune-enzymatic assay (ELISA), after the treatment with 1, 10 and 100 μM triamcinolone compared to no treated controls at the time points 6, 12, 18, 24, 48, 72 and 96 h, and to PRP at 48, 72 and 96 h. Results: The cytokines IL-6 and IL-8 were produced in high concentrations by LEE cells. One, 10 and 100 μM triamcinolone induced significant decrease in the production of IL-6 and IL-8 at 48, 72 and 96 h, adding the time point 12 h for IL-8. Compared to controls, PRP caused a significant increase in the production of IL-6 and IL-8 and there was a significant increase in IL-10 production with the use of 100 μM triamcinolone at 48 h. The production of IL1-β and TNF-α was very low and did not change when the cultures were treated with triamcinolone or PRP. Conclusion: LEE-derived cells produce IL-6 and IL-8, confirming the inflammatory nature of this condition. While triamcinolone inhibited the production of IL-6 and IL-8 by LEE cells, PRP induced an increase in these cytokines compared with controls.
publishDate 2022
dc.date.none.fl_str_mv 2022-04-29T08:39:35Z
2022-04-29T08:39:35Z
2022-12-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1186/s13018-022-02990-0
Journal of Orthopaedic Surgery and Research, v. 17, n. 1, 2022.
1749-799X
http://hdl.handle.net/11449/230393
10.1186/s13018-022-02990-0
2-s2.0-85124680382
url http://dx.doi.org/10.1186/s13018-022-02990-0
http://hdl.handle.net/11449/230393
identifier_str_mv Journal of Orthopaedic Surgery and Research, v. 17, n. 1, 2022.
1749-799X
10.1186/s13018-022-02990-0
2-s2.0-85124680382
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal of Orthopaedic Surgery and Research
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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