Avaliação do metabolismo oxidativo em cepa Y de Trypanosoma cruzi tratadas com o composto derivado do benzofuroxânico LAPDESF - BZFS

• Autor(a) principal: Silva, Flávia Alves de Jesus [UNESP]
• Data de Publicação: 2015
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://hdl.handle.net/11449/143027
• topic: Trypanosoma cruzi; Stress oxidativo; Enzimas; Tripanossomose; Superoxido desmutase; Doenças parasitarias; Química farmacêutica; Western blotting

The American trypanosomiasis, also known for Chagas disease, and transmitted for infected triatomine by etiological agent Trypanosoma cruzi. Since it was first described in 1909, this disease has expanded the world infecting millions of people, mostly in Latin America, where it is endemic. Currently only two drugs are available for Chagas disease treatment, nifurtimox, which is not used in Brazil, and benznidazole, which had its much reduced industrial production. However, both drugs have limited actions. Thus, the search for new compounds and to understand the mechanism of resistance to trypanocidal drugs available and that are still in the research phase, have been the subject of several studies. Some studies report an increase in the production of enzymes that action in cellular defense, which probably could be responsible for parasite resistance. Some enzymes have important functions in the survival and growth of parasites: superoxide dismutase (SOD), metalloenzyme that eliminates superoxide radicals to convert them hydrogen peroxide and molecular oxygen; old yellow enzyme (OYE) a NADPH- flavin oxidoreductase that may be involved in the reduction of trypanocidal substances and peroxiredoxin (Prx), which catalyzes the reduction of peroxides. By means of Western blotting analyzed for changes in expression level of SOD, Prx and OYE in strains of T. cruzi treated or not with a compound derived benzofuroxânico, LAPDESFBZFS ( 6 - (( methyl) benzo [c ] (2- carbamoilidrazona ) -1 -N-oxide -1,2,5- oxadiazol ). The CI50 (cytotoxicity index) of this compound was 6.88 µg/mL, and promoted changes in the expression of PRX and OYE enzymes, probably by promoting oxidative stress in epimastigotes .