Efeito do plasma rico em glicose na apoptose de neutrófilos de cães

• Autor(a) principal: Ramos Nogueira, Maria Carolina [UNESP]
• Data de Publicação: 2013
• Outros Autores: Pereira, Priscila Preve [UNESP], Martins Almeida, Breno Fernando [UNESP], Bosco, Anelise Maria [UNESP], Barbosa, Tatiana Sousa [UNESP], Ciarlini, Paulo César [UNESP]
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://hdl.handle.net/11449/232230
• topic: Hyperglycemia; Immunosuppression; Polymorphonuclear

Hyperglycemia is considered the main cause of increased mortality in diabetic humans, to promote the acceleration of neutrophil apoptosis, essential for the functioning of the immune system. Thus, there is an immunosuppressive effect that contributes to the increased susceptibility of patients to serious bacterial infections. In canine species there is no similar studies on the immunosuppressive effect of hyperglycemia, so that it tested the hypothesis that excess glucose accelerates neutrophil apoptosis in dogs. To this end, replaced the plasma of blood samples from 10 healthy dogs plasma autologous, homologous counterpart normoglycemia and hyperglycemia. All samples were incubated for two and four hours.The glucose measurements were performed using kinetic glucose oxidase and neutrophil apoptotic index calculated by the morphometric method. In all tests the apoptotic index increased after two and four hours. After four hours, the apoptotic index of the samples incubated with homologous plasma hyperglycemia was significantly higher (p >0.05) to the samples incubated with homologous and autologous normoglycemia. It was concluded that plasma hyperglycemia in vitro increases the rate of neutrophil apoptosis in dogs, strengthening the hypothesis that dogs with hyperglycemic disease may also suffer immunosuppression neutrophil dysfunction.