Overproduction and properties of lipase by a wild strain of Burkholderia lata LBBIO-BL02 using chicken fat
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s13213-014-0928-6 http://hdl.handle.net/11449/171857 |
Resumo: | In this work, we report the overproduction of lipases by a new wild strain of Burkholderia lata (LBBIO-BL02) in submerged fermentation to seek an economically attractive bioprocess. The best fermentation medium composition was containing chicken fat (12.5 mL/L) and ammonium phosphate (15 g/L) at 35 ºC and pH 7.0, resulting in a lipase titer of 1137.82 U/mL and 2146.83 U/mg. The lipase characterization exhibited maximum activity at 55 °C and pH 8.0. The lipase retained 100, 93 and 85 % of its maximum activity at 45, 50 and 60 °C, respectively, and 78, 82 and 100 % at pH 2.2, 3 and 10, respectively. The enzyme was successfully immobilized on Celite by adsorption and showed a promising future for various organic syntheses because of its stability in organic solvents. All the results shows that Burkholderia lata LBBIO-BL02 is a superior lipase-producing bacterium and its enzyme showed good potential for industrial and biotechnology application. |
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Overproduction and properties of lipase by a wild strain of Burkholderia lata LBBIO-BL02 using chicken fatBioprocess designCeliteChicken fatImmobilizationLipaseOverproductionIn this work, we report the overproduction of lipases by a new wild strain of Burkholderia lata (LBBIO-BL02) in submerged fermentation to seek an economically attractive bioprocess. The best fermentation medium composition was containing chicken fat (12.5 mL/L) and ammonium phosphate (15 g/L) at 35 ºC and pH 7.0, resulting in a lipase titer of 1137.82 U/mL and 2146.83 U/mg. The lipase characterization exhibited maximum activity at 55 °C and pH 8.0. The lipase retained 100, 93 and 85 % of its maximum activity at 45, 50 and 60 °C, respectively, and 78, 82 and 100 % at pH 2.2, 3 and 10, respectively. The enzyme was successfully immobilized on Celite by adsorption and showed a promising future for various organic syntheses because of its stability in organic solvents. All the results shows that Burkholderia lata LBBIO-BL02 is a superior lipase-producing bacterium and its enzyme showed good potential for industrial and biotechnology application.Departamento de Ciências Biológicas Laboratório de Biotecnologia Industrial Faculdade de Ciências e Letras UNESP - Universidade Estadual Paulista Campus de AssisDepartamento de Bioquímica e Microbiologia Instituto de Biociências UNESP – Universidade Estadual Paulista, Campus de Rio ClaroLaboratório de Bioquímica e Bioprocessos Depto. de Ciências Biológicas Universidade Estadual Paulista (UNESP), Campus de Assis, Av. Dom Antônio, 2100Departamento de Ciências Biológicas Laboratório de Biotecnologia Industrial Faculdade de Ciências e Letras UNESP - Universidade Estadual Paulista Campus de AssisDepartamento de Bioquímica e Microbiologia Instituto de Biociências UNESP – Universidade Estadual Paulista, Campus de Rio ClaroLaboratório de Bioquímica e Bioprocessos Depto. de Ciências Biológicas Universidade Estadual Paulista (UNESP), Campus de Assis, Av. Dom Antônio, 2100Universidade Estadual Paulista (Unesp)Oliveira, Bruno H. [UNESP]Santos, Rachid É. [UNESP]Loiola, Luiz E. A. [UNESP]Nascimento, Valeria M. G. [UNESP]2018-12-11T16:57:28Z2018-12-11T16:57:28Z2015-06-26info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article865-877application/pdfhttp://dx.doi.org/10.1007/s13213-014-0928-6Annals of Microbiology, v. 65, n. 2, p. 865-877, 2015.1869-20441590-4261http://hdl.handle.net/11449/17185710.1007/s13213-014-0928-62-s2.0-849298442552-s2.0-84929844255.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAnnals of Microbiology0,479info:eu-repo/semantics/openAccess2024-06-13T17:38:42Zoai:repositorio.unesp.br:11449/171857Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:14:16.292946Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Overproduction and properties of lipase by a wild strain of Burkholderia lata LBBIO-BL02 using chicken fat |
title |
Overproduction and properties of lipase by a wild strain of Burkholderia lata LBBIO-BL02 using chicken fat |
spellingShingle |
Overproduction and properties of lipase by a wild strain of Burkholderia lata LBBIO-BL02 using chicken fat Oliveira, Bruno H. [UNESP] Bioprocess design Celite Chicken fat Immobilization Lipase Overproduction |
title_short |
Overproduction and properties of lipase by a wild strain of Burkholderia lata LBBIO-BL02 using chicken fat |
title_full |
Overproduction and properties of lipase by a wild strain of Burkholderia lata LBBIO-BL02 using chicken fat |
title_fullStr |
Overproduction and properties of lipase by a wild strain of Burkholderia lata LBBIO-BL02 using chicken fat |
title_full_unstemmed |
Overproduction and properties of lipase by a wild strain of Burkholderia lata LBBIO-BL02 using chicken fat |
title_sort |
Overproduction and properties of lipase by a wild strain of Burkholderia lata LBBIO-BL02 using chicken fat |
author |
Oliveira, Bruno H. [UNESP] |
author_facet |
Oliveira, Bruno H. [UNESP] Santos, Rachid É. [UNESP] Loiola, Luiz E. A. [UNESP] Nascimento, Valeria M. G. [UNESP] |
author_role |
author |
author2 |
Santos, Rachid É. [UNESP] Loiola, Luiz E. A. [UNESP] Nascimento, Valeria M. G. [UNESP] |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Oliveira, Bruno H. [UNESP] Santos, Rachid É. [UNESP] Loiola, Luiz E. A. [UNESP] Nascimento, Valeria M. G. [UNESP] |
dc.subject.por.fl_str_mv |
Bioprocess design Celite Chicken fat Immobilization Lipase Overproduction |
topic |
Bioprocess design Celite Chicken fat Immobilization Lipase Overproduction |
description |
In this work, we report the overproduction of lipases by a new wild strain of Burkholderia lata (LBBIO-BL02) in submerged fermentation to seek an economically attractive bioprocess. The best fermentation medium composition was containing chicken fat (12.5 mL/L) and ammonium phosphate (15 g/L) at 35 ºC and pH 7.0, resulting in a lipase titer of 1137.82 U/mL and 2146.83 U/mg. The lipase characterization exhibited maximum activity at 55 °C and pH 8.0. The lipase retained 100, 93 and 85 % of its maximum activity at 45, 50 and 60 °C, respectively, and 78, 82 and 100 % at pH 2.2, 3 and 10, respectively. The enzyme was successfully immobilized on Celite by adsorption and showed a promising future for various organic syntheses because of its stability in organic solvents. All the results shows that Burkholderia lata LBBIO-BL02 is a superior lipase-producing bacterium and its enzyme showed good potential for industrial and biotechnology application. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-06-26 2018-12-11T16:57:28Z 2018-12-11T16:57:28Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s13213-014-0928-6 Annals of Microbiology, v. 65, n. 2, p. 865-877, 2015. 1869-2044 1590-4261 http://hdl.handle.net/11449/171857 10.1007/s13213-014-0928-6 2-s2.0-84929844255 2-s2.0-84929844255.pdf |
url |
http://dx.doi.org/10.1007/s13213-014-0928-6 http://hdl.handle.net/11449/171857 |
identifier_str_mv |
Annals of Microbiology, v. 65, n. 2, p. 865-877, 2015. 1869-2044 1590-4261 10.1007/s13213-014-0928-6 2-s2.0-84929844255 2-s2.0-84929844255.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Annals of Microbiology 0,479 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
865-877 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808129300325466112 |