Immune reconstitution in HIV-1 infected patients treated for two years with highly active antiretroviral therapy

Detalhes bibliográficos
Autor(a) principal: Almeida, Ricardo Augusto Monteiro de Barros [UNESP]
Data de Publicação: 2006
Outros Autores: Souza, L. R. [UNESP], Calvi, Sueli Aparecida [UNESP], Ikoma, M. R V, Silva, V. A., Curi, P. R. [UNESP], Meira, D. A. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/S1678-91992006000100008
http://hdl.handle.net/11449/68852
Resumo: The aim of this paper was to evaluate the immune reconstitution of HIV-1 patients subjected to highly active antiretroviral therapy (HAART) for two years or more according to CD 45RA and CD 45RO cell count; determination of IL-2, IFN-γ, IL-4, IL-10 and TNF-α serum levels; CD 4 + T and CD 8 + T lymphocyte count; and plasma viral load (VL) determination. For this purpose, a cross sectional study was carried out in the Tropical Diseases Area, Botucatu School of Medicine, São Paulo State University, UNESP, Botucatu, São Paulo, Brazil. Between June 2001 and April 2002, 37 HIV-1 infected patients were evaluated, 13 with treatment indication but untreated (G1), 9 subjected to HAART for 5-7 months (G2), and 15 treated for two years or more (G3); both treated groups used medication regularly and without failure. Forty-nine normal individuals were studied as controls (GC-1 and GC-2). There was a tendency (p<0.10) for the predominance of two nucleoside reverse transcriptase inhibitors (NRTI) associated with one non-nucleoside reverse transcriptase inhibitor (NNRTI) regimen in G2; and two NRTI associated with a protease inhibitor (PI) in G3. Statistical differences between groups were seen for CD 45RA (G1<[G3=GC-2]; p<0.05) and CD 45RO (G1<GC-2<G3; p<0.01) cells, and CD 4 + T lymphocyte count (G1<G3; G2-intermediate; p<0.05), VL determination (G1>[G2=G3]; p<0.001), TNF-α serum determination ([G1>G3; G2=intermediate]>GC-1; p<0.001), IL-2 (G1<[G2=G3=GC-1]; p<0.01), IFN-γ ([G1=GC-1]>[GC-2=G3]; p<0.001), IL-4 and IL-10 ([G1=G2=G3]>GC-1; p<0.001), serum cytokine profiles, with a higher proportion of subtype 2 in G1 and mature subtype 0 in G2 and G3 (p<0.005). There was no statistical difference for CD 8 + T lymphocyte counts (G1=G2=G3; p<0.50). Consistency was seen between positive correlations of profile 1 definer cytokines (IL-2 and IFN-γ), CD 45RA and CD 45RO cells, and CD 4 + T lymphocyte counts and between positive correlations of profile 2 definer cytokines (IL-4 and IL-10) with TNF-α, and VL. The negative correlations were also consistent as they expressed the inverse of the positives. The variables with the highest number of correlations were IL-2, IFN-γ, and VL, followed by CD 45RA and CD 45RO cells, and IL-10. The variables with the lowest number of correlations were CD 4 + T and CD 8 + T lymphocytes. The results express the partial but important immune reconstitution in HIV-1 infected individuals with the interference of HAART and the importance of cytokines especially IL-2 and IFN-γ, and CD 45RA and CD 45RO cells as surrogate markers of this reconstitution.
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spelling Immune reconstitution in HIV-1 infected patients treated for two years with highly active antiretroviral therapyAntiretroviralCD 45RACd 45ROCytokinesHAARTHIVImmune reconstitutionHuman immunodeficiency virusHuman immunodeficiency virus 1The aim of this paper was to evaluate the immune reconstitution of HIV-1 patients subjected to highly active antiretroviral therapy (HAART) for two years or more according to CD 45RA and CD 45RO cell count; determination of IL-2, IFN-γ, IL-4, IL-10 and TNF-α serum levels; CD 4 + T and CD 8 + T lymphocyte count; and plasma viral load (VL) determination. For this purpose, a cross sectional study was carried out in the Tropical Diseases Area, Botucatu School of Medicine, São Paulo State University, UNESP, Botucatu, São Paulo, Brazil. Between June 2001 and April 2002, 37 HIV-1 infected patients were evaluated, 13 with treatment indication but untreated (G1), 9 subjected to HAART for 5-7 months (G2), and 15 treated for two years or more (G3); both treated groups used medication regularly and without failure. Forty-nine normal individuals were studied as controls (GC-1 and GC-2). There was a tendency (p<0.10) for the predominance of two nucleoside reverse transcriptase inhibitors (NRTI) associated with one non-nucleoside reverse transcriptase inhibitor (NNRTI) regimen in G2; and two NRTI associated with a protease inhibitor (PI) in G3. Statistical differences between groups were seen for CD 45RA (G1<[G3=GC-2]; p<0.05) and CD 45RO (G1<GC-2<G3; p<0.01) cells, and CD 4 + T lymphocyte count (G1<G3; G2-intermediate; p<0.05), VL determination (G1>[G2=G3]; p<0.001), TNF-α serum determination ([G1>G3; G2=intermediate]>GC-1; p<0.001), IL-2 (G1<[G2=G3=GC-1]; p<0.01), IFN-γ ([G1=GC-1]>[GC-2=G3]; p<0.001), IL-4 and IL-10 ([G1=G2=G3]>GC-1; p<0.001), serum cytokine profiles, with a higher proportion of subtype 2 in G1 and mature subtype 0 in G2 and G3 (p<0.005). There was no statistical difference for CD 8 + T lymphocyte counts (G1=G2=G3; p<0.50). Consistency was seen between positive correlations of profile 1 definer cytokines (IL-2 and IFN-γ), CD 45RA and CD 45RO cells, and CD 4 + T lymphocyte counts and between positive correlations of profile 2 definer cytokines (IL-4 and IL-10) with TNF-α, and VL. The negative correlations were also consistent as they expressed the inverse of the positives. The variables with the highest number of correlations were IL-2, IFN-γ, and VL, followed by CD 45RA and CD 45RO cells, and IL-10. The variables with the lowest number of correlations were CD 4 + T and CD 8 + T lymphocytes. The results express the partial but important immune reconstitution in HIV-1 infected individuals with the interference of HAART and the importance of cytokines especially IL-2 and IFN-γ, and CD 45RA and CD 45RO cells as surrogate markers of this reconstitution.Department of Tropical Diseases and Imaging Diagnosis Botucatu School of Medicine São Paulo State University, Botucatu, São PauloApplied Immunology Laboratory of Tropical Diseases Botucatu School of Medicine São Paulo State University, Botucatu, São PauloBotucatu Hemocenter, Botucatu, São PauloBotucatu School of Veterinary Medicine and Animal Husbandry São Paulo State University UNESP, Botucatu, São PauloDepartamento de Doenças Tropicais e Diagnóstico Por Imagem Faculdade de Medicina de Botucatu UNESP, Distrito de Rubião Jr., S/N, 18618-000, Botucatu, SPDepartment of Tropical Diseases and Imaging Diagnosis Botucatu School of Medicine São Paulo State University, Botucatu, São PauloApplied Immunology Laboratory of Tropical Diseases Botucatu School of Medicine São Paulo State University, Botucatu, São PauloBotucatu School of Veterinary Medicine and Animal Husbandry São Paulo State University UNESP, Botucatu, São PauloDepartamento de Doenças Tropicais e Diagnóstico Por Imagem Faculdade de Medicina de Botucatu UNESP, Distrito de Rubião Jr., S/N, 18618-000, Botucatu, SPUniversidade Estadual Paulista (Unesp)Almeida, Ricardo Augusto Monteiro de Barros [UNESP]Souza, L. R. [UNESP]Calvi, Sueli Aparecida [UNESP]Ikoma, M. R VSilva, V. A.Curi, P. R. [UNESP]Meira, D. A. [UNESP]2014-05-27T11:21:51Z2014-05-27T11:21:51Z2006-04-26info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article91-109application/pdfhttp://dx.doi.org/10.1590/S1678-91992006000100008Journal of Venomous Animals and Toxins Including Tropical Diseases, v. 12, n. 1, p. 91-109, 2006.1678-9199http://hdl.handle.net/11449/6885210.1590/S1678-91992006000100008S1678-91992006000100008WOS:0002462810000082-s2.0-336458844282-s2.0-33645884428.pdf217945002269905902081384501218980000-0003-0567-6221Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Venomous Animals and Toxins Including Tropical Diseases1.7820,573info:eu-repo/semantics/openAccess2023-10-08T06:04:16Zoai:repositorio.unesp.br:11449/68852Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-10-08T06:04:16Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Immune reconstitution in HIV-1 infected patients treated for two years with highly active antiretroviral therapy
title Immune reconstitution in HIV-1 infected patients treated for two years with highly active antiretroviral therapy
spellingShingle Immune reconstitution in HIV-1 infected patients treated for two years with highly active antiretroviral therapy
Almeida, Ricardo Augusto Monteiro de Barros [UNESP]
Antiretroviral
CD 45RA
Cd 45RO
Cytokines
HAART
HIV
Immune reconstitution
Human immunodeficiency virus
Human immunodeficiency virus 1
title_short Immune reconstitution in HIV-1 infected patients treated for two years with highly active antiretroviral therapy
title_full Immune reconstitution in HIV-1 infected patients treated for two years with highly active antiretroviral therapy
title_fullStr Immune reconstitution in HIV-1 infected patients treated for two years with highly active antiretroviral therapy
title_full_unstemmed Immune reconstitution in HIV-1 infected patients treated for two years with highly active antiretroviral therapy
title_sort Immune reconstitution in HIV-1 infected patients treated for two years with highly active antiretroviral therapy
author Almeida, Ricardo Augusto Monteiro de Barros [UNESP]
author_facet Almeida, Ricardo Augusto Monteiro de Barros [UNESP]
Souza, L. R. [UNESP]
Calvi, Sueli Aparecida [UNESP]
Ikoma, M. R V
Silva, V. A.
Curi, P. R. [UNESP]
Meira, D. A. [UNESP]
author_role author
author2 Souza, L. R. [UNESP]
Calvi, Sueli Aparecida [UNESP]
Ikoma, M. R V
Silva, V. A.
Curi, P. R. [UNESP]
Meira, D. A. [UNESP]
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Almeida, Ricardo Augusto Monteiro de Barros [UNESP]
Souza, L. R. [UNESP]
Calvi, Sueli Aparecida [UNESP]
Ikoma, M. R V
Silva, V. A.
Curi, P. R. [UNESP]
Meira, D. A. [UNESP]
dc.subject.por.fl_str_mv Antiretroviral
CD 45RA
Cd 45RO
Cytokines
HAART
HIV
Immune reconstitution
Human immunodeficiency virus
Human immunodeficiency virus 1
topic Antiretroviral
CD 45RA
Cd 45RO
Cytokines
HAART
HIV
Immune reconstitution
Human immunodeficiency virus
Human immunodeficiency virus 1
description The aim of this paper was to evaluate the immune reconstitution of HIV-1 patients subjected to highly active antiretroviral therapy (HAART) for two years or more according to CD 45RA and CD 45RO cell count; determination of IL-2, IFN-γ, IL-4, IL-10 and TNF-α serum levels; CD 4 + T and CD 8 + T lymphocyte count; and plasma viral load (VL) determination. For this purpose, a cross sectional study was carried out in the Tropical Diseases Area, Botucatu School of Medicine, São Paulo State University, UNESP, Botucatu, São Paulo, Brazil. Between June 2001 and April 2002, 37 HIV-1 infected patients were evaluated, 13 with treatment indication but untreated (G1), 9 subjected to HAART for 5-7 months (G2), and 15 treated for two years or more (G3); both treated groups used medication regularly and without failure. Forty-nine normal individuals were studied as controls (GC-1 and GC-2). There was a tendency (p<0.10) for the predominance of two nucleoside reverse transcriptase inhibitors (NRTI) associated with one non-nucleoside reverse transcriptase inhibitor (NNRTI) regimen in G2; and two NRTI associated with a protease inhibitor (PI) in G3. Statistical differences between groups were seen for CD 45RA (G1<[G3=GC-2]; p<0.05) and CD 45RO (G1<GC-2<G3; p<0.01) cells, and CD 4 + T lymphocyte count (G1<G3; G2-intermediate; p<0.05), VL determination (G1>[G2=G3]; p<0.001), TNF-α serum determination ([G1>G3; G2=intermediate]>GC-1; p<0.001), IL-2 (G1<[G2=G3=GC-1]; p<0.01), IFN-γ ([G1=GC-1]>[GC-2=G3]; p<0.001), IL-4 and IL-10 ([G1=G2=G3]>GC-1; p<0.001), serum cytokine profiles, with a higher proportion of subtype 2 in G1 and mature subtype 0 in G2 and G3 (p<0.005). There was no statistical difference for CD 8 + T lymphocyte counts (G1=G2=G3; p<0.50). Consistency was seen between positive correlations of profile 1 definer cytokines (IL-2 and IFN-γ), CD 45RA and CD 45RO cells, and CD 4 + T lymphocyte counts and between positive correlations of profile 2 definer cytokines (IL-4 and IL-10) with TNF-α, and VL. The negative correlations were also consistent as they expressed the inverse of the positives. The variables with the highest number of correlations were IL-2, IFN-γ, and VL, followed by CD 45RA and CD 45RO cells, and IL-10. The variables with the lowest number of correlations were CD 4 + T and CD 8 + T lymphocytes. The results express the partial but important immune reconstitution in HIV-1 infected individuals with the interference of HAART and the importance of cytokines especially IL-2 and IFN-γ, and CD 45RA and CD 45RO cells as surrogate markers of this reconstitution.
publishDate 2006
dc.date.none.fl_str_mv 2006-04-26
2014-05-27T11:21:51Z
2014-05-27T11:21:51Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S1678-91992006000100008
Journal of Venomous Animals and Toxins Including Tropical Diseases, v. 12, n. 1, p. 91-109, 2006.
1678-9199
http://hdl.handle.net/11449/68852
10.1590/S1678-91992006000100008
S1678-91992006000100008
WOS:000246281000008
2-s2.0-33645884428
2-s2.0-33645884428.pdf
2179450022699059
0208138450121898
0000-0003-0567-6221
url http://dx.doi.org/10.1590/S1678-91992006000100008
http://hdl.handle.net/11449/68852
identifier_str_mv Journal of Venomous Animals and Toxins Including Tropical Diseases, v. 12, n. 1, p. 91-109, 2006.
1678-9199
10.1590/S1678-91992006000100008
S1678-91992006000100008
WOS:000246281000008
2-s2.0-33645884428
2-s2.0-33645884428.pdf
2179450022699059
0208138450121898
0000-0003-0567-6221
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal of Venomous Animals and Toxins Including Tropical Diseases
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dc.format.none.fl_str_mv 91-109
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dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
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