Mortality of Conotrachelus humeropictus in response to combined application of the nematode Steinernema brazilense and the fungus Beauveria bassiana

Detalhes bibliográficos
Autor(a) principal: Simi, Lucas Detogni [UNESP]
Data de Publicação: 2018
Outros Autores: Leite, Luís Garrigós, Trevisan, Olzeno, Costa, José Nilton Medeiros, Oliveira, Leandro Ezequiel, Schmidt, Fabio Silber, Bueno, Roselaine Nunes Da Silva, Batista, Antonio
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/1808-1657000092016
http://hdl.handle.net/11449/212031
Resumo: The fruit borer Conotrachelus humeropictus is one of the most important pests of the cupuaçu crop, and can promote yield losses of around 50%. The present study involved the selection of Beauveria bassiana isolates on Conotrachelus psidii larvae, which was used as test insect due to its taxonomic closeness to C. humeropictus. In order to assess their interaction using combined application, we studied the ability of the nematode to carry conidia of B. bassiana and Metarhizium anisopliae to soil layers. This study also aimed to establish a biological control method for cupuaçu borer with the use of the fungus B. bassiana at 10 kg/ha and the entomopathogenic nematode Steinernema brazilense at 1, 3, 9, and 27 IJ/cm2, applied as single and combined treatments. In the selection of B. bassiana strains for C. psidii, the IBCB 276 and IBCB 165 isolates were the most promising ones, causing 86 and 84% mortality, respectively. The IBCB 276 strain was applied in the field to control C. humeropictus. In the study of carriage of conidia in soil, the nematode S. brazilense was found to be capable of carrying conidia of B. bassiana to deep 7 to 10 cm soil layers. This was not observed with M. anisopliae, found only in the topsoil. In field trials against C. humeropictus larvae, B. bassiana promoted 15.6% mortality. The nematode promoted 60.0% mortality at the highest concentration used, and their combined use with B. bassiana (10 kg/ha) increased mortality to 65.6%, with an additive interaction observed between the two entomopathogens.
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spelling Mortality of Conotrachelus humeropictus in response to combined application of the nematode Steinernema brazilense and the fungus Beauveria bassianaMortalidade de Conotrachelus humeropictus pela aplicação combinada do nematoide Steinernema brazilense com o fungo entomopatogênico Beauveria bassianabiological controlmicrobial controlinteractioncombined usecontrole biológicocontrole microbianointeraçãouso combinadoThe fruit borer Conotrachelus humeropictus is one of the most important pests of the cupuaçu crop, and can promote yield losses of around 50%. The present study involved the selection of Beauveria bassiana isolates on Conotrachelus psidii larvae, which was used as test insect due to its taxonomic closeness to C. humeropictus. In order to assess their interaction using combined application, we studied the ability of the nematode to carry conidia of B. bassiana and Metarhizium anisopliae to soil layers. This study also aimed to establish a biological control method for cupuaçu borer with the use of the fungus B. bassiana at 10 kg/ha and the entomopathogenic nematode Steinernema brazilense at 1, 3, 9, and 27 IJ/cm2, applied as single and combined treatments. In the selection of B. bassiana strains for C. psidii, the IBCB 276 and IBCB 165 isolates were the most promising ones, causing 86 and 84% mortality, respectively. The IBCB 276 strain was applied in the field to control C. humeropictus. In the study of carriage of conidia in soil, the nematode S. brazilense was found to be capable of carrying conidia of B. bassiana to deep 7 to 10 cm soil layers. This was not observed with M. anisopliae, found only in the topsoil. In field trials against C. humeropictus larvae, B. bassiana promoted 15.6% mortality. The nematode promoted 60.0% mortality at the highest concentration used, and their combined use with B. bassiana (10 kg/ha) increased mortality to 65.6%, with an additive interaction observed between the two entomopathogens.A broca-do-cupuaçu, Conotrachelus humeropictus, é uma das principais pragas da cultura, podendo ocasionar perdas de 50% na produção. O presente trabalho envolveu uma seleção prévia de isolados de Beauveria bassiana em larvas de Conotrachelus psidii, utilizado como inseto teste por ser próximo taxonomicamente à C. humeropictus. Com o objetivo de avaliar causa-efeitos da interação na aplicação combinada, foi estudada a capacidade do nematoide carrear conídios de B. bassiana e Metarhizium anisopliae pelas camadas de solo. O trabalho também objetivou avaliar o controle biológico da broca-do-cupuaçu, através do uso do fungo B. bassiana 10 kg/ha, e do nematoide entomopatogênico Steinernema brazilense 1, 3, 9 e 27 JI/cm2, aplicados em tratamentos isolados e em combinação. Na seleção de isolados de B. bassiana para C. psidii, os isolados IBCB 276 e IBCB 165 foram os mais promissores, causando, respectivamente, 86 e 84% de mortalidade confirmada. O isolado IBCB 276 foi aplicado em campo para controlar C. humeropictus. No estudo de carreamento de conídios, o nematoide S. brazilense foi capaz de carrear conídios de B. bassiana para a camada de 7 a 10 cm de profundidade no solo. A mesma ação não foi observada com M. anisopliae, encontrado somente na camada superficial do solo. Nos ensaios de campo contra C. humeropictus, B. bassiana promoveu 15,6% de mortalidade. O nematoide promoveu 60,0% de mortalidade na maior concentração utilizada e, na combinação dessa concentração do NEP com B. bassiana (10 kg/ha), a mortalidade atingiu 65,6%, observando-se interação aditiva entre os dois entomopatógenos.Universidade Estadual Paulista “Júlio de Mesquita Filho”, Campus de BotucatuInstituto BiológicoEMBRAPA Rondônia, CEPLAC - Comissão Executiva do Plano da Lavoura CacaueiraCEPLAC - Comissão Executiva do Plano da Lavoura CacaueiraBio Controle, Métodos de controle de pragas LtdaUniversidade Estadual Paulista “Júlio de Mesquita Filho”, Campus de BotucatuInstituto BiológicoUniversidade Estadual Paulista (Unesp)Instituto BiológicoEMBRAPA RondôniaCEPLAC - Comissão Executiva do Plano da Lavoura CacaueiraBio ControleSimi, Lucas Detogni [UNESP]Leite, Luís GarrigósTrevisan, OlzenoCosta, José Nilton MedeirosOliveira, Leandro EzequielSchmidt, Fabio SilberBueno, Roselaine Nunes Da SilvaBatista, Antonio2021-07-14T10:33:24Z2021-07-14T10:33:24Z2018-08-23info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article-application/pdfhttp://dx.doi.org/10.1590/1808-1657000092016Arquivos do Instituto Biológico. Instituto Biológico, v. 85, p. -, 2018.0020-36531808-1657http://hdl.handle.net/11449/21203110.1590/1808-1657000092016S1808-16572018000100207S1808-16572018000100207.pdfSciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengArquivos do Instituto Biológicoinfo:eu-repo/semantics/openAccess2024-01-26T06:34:39Zoai:repositorio.unesp.br:11449/212031Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-06T00:02:29.503234Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Mortality of Conotrachelus humeropictus in response to combined application of the nematode Steinernema brazilense and the fungus Beauveria bassiana
Mortalidade de Conotrachelus humeropictus pela aplicação combinada do nematoide Steinernema brazilense com o fungo entomopatogênico Beauveria bassiana
title Mortality of Conotrachelus humeropictus in response to combined application of the nematode Steinernema brazilense and the fungus Beauveria bassiana
spellingShingle Mortality of Conotrachelus humeropictus in response to combined application of the nematode Steinernema brazilense and the fungus Beauveria bassiana
Simi, Lucas Detogni [UNESP]
biological control
microbial control
interaction
combined use
controle biológico
controle microbiano
interação
uso combinado
title_short Mortality of Conotrachelus humeropictus in response to combined application of the nematode Steinernema brazilense and the fungus Beauveria bassiana
title_full Mortality of Conotrachelus humeropictus in response to combined application of the nematode Steinernema brazilense and the fungus Beauveria bassiana
title_fullStr Mortality of Conotrachelus humeropictus in response to combined application of the nematode Steinernema brazilense and the fungus Beauveria bassiana
title_full_unstemmed Mortality of Conotrachelus humeropictus in response to combined application of the nematode Steinernema brazilense and the fungus Beauveria bassiana
title_sort Mortality of Conotrachelus humeropictus in response to combined application of the nematode Steinernema brazilense and the fungus Beauveria bassiana
author Simi, Lucas Detogni [UNESP]
author_facet Simi, Lucas Detogni [UNESP]
Leite, Luís Garrigós
Trevisan, Olzeno
Costa, José Nilton Medeiros
Oliveira, Leandro Ezequiel
Schmidt, Fabio Silber
Bueno, Roselaine Nunes Da Silva
Batista, Antonio
author_role author
author2 Leite, Luís Garrigós
Trevisan, Olzeno
Costa, José Nilton Medeiros
Oliveira, Leandro Ezequiel
Schmidt, Fabio Silber
Bueno, Roselaine Nunes Da Silva
Batista, Antonio
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Instituto Biológico
EMBRAPA Rondônia
CEPLAC - Comissão Executiva do Plano da Lavoura Cacaueira
Bio Controle
dc.contributor.author.fl_str_mv Simi, Lucas Detogni [UNESP]
Leite, Luís Garrigós
Trevisan, Olzeno
Costa, José Nilton Medeiros
Oliveira, Leandro Ezequiel
Schmidt, Fabio Silber
Bueno, Roselaine Nunes Da Silva
Batista, Antonio
dc.subject.por.fl_str_mv biological control
microbial control
interaction
combined use
controle biológico
controle microbiano
interação
uso combinado
topic biological control
microbial control
interaction
combined use
controle biológico
controle microbiano
interação
uso combinado
description The fruit borer Conotrachelus humeropictus is one of the most important pests of the cupuaçu crop, and can promote yield losses of around 50%. The present study involved the selection of Beauveria bassiana isolates on Conotrachelus psidii larvae, which was used as test insect due to its taxonomic closeness to C. humeropictus. In order to assess their interaction using combined application, we studied the ability of the nematode to carry conidia of B. bassiana and Metarhizium anisopliae to soil layers. This study also aimed to establish a biological control method for cupuaçu borer with the use of the fungus B. bassiana at 10 kg/ha and the entomopathogenic nematode Steinernema brazilense at 1, 3, 9, and 27 IJ/cm2, applied as single and combined treatments. In the selection of B. bassiana strains for C. psidii, the IBCB 276 and IBCB 165 isolates were the most promising ones, causing 86 and 84% mortality, respectively. The IBCB 276 strain was applied in the field to control C. humeropictus. In the study of carriage of conidia in soil, the nematode S. brazilense was found to be capable of carrying conidia of B. bassiana to deep 7 to 10 cm soil layers. This was not observed with M. anisopliae, found only in the topsoil. In field trials against C. humeropictus larvae, B. bassiana promoted 15.6% mortality. The nematode promoted 60.0% mortality at the highest concentration used, and their combined use with B. bassiana (10 kg/ha) increased mortality to 65.6%, with an additive interaction observed between the two entomopathogens.
publishDate 2018
dc.date.none.fl_str_mv 2018-08-23
2021-07-14T10:33:24Z
2021-07-14T10:33:24Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/1808-1657000092016
Arquivos do Instituto Biológico. Instituto Biológico, v. 85, p. -, 2018.
0020-3653
1808-1657
http://hdl.handle.net/11449/212031
10.1590/1808-1657000092016
S1808-16572018000100207
S1808-16572018000100207.pdf
url http://dx.doi.org/10.1590/1808-1657000092016
http://hdl.handle.net/11449/212031
identifier_str_mv Arquivos do Instituto Biológico. Instituto Biológico, v. 85, p. -, 2018.
0020-3653
1808-1657
10.1590/1808-1657000092016
S1808-16572018000100207
S1808-16572018000100207.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Arquivos do Instituto Biológico
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv -
application/pdf
dc.publisher.none.fl_str_mv Instituto Biológico
publisher.none.fl_str_mv Instituto Biológico
dc.source.none.fl_str_mv SciELO
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
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repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
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