Natural Theileria equi infection in captive Tapirus terrestris (Perissodactyla: Tapiridae) in the Brazilian Amazon
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2020 |
| Outros Autores: | , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da UNESP |
| Texto Completo: | http://dx.doi.org/10.1016/j.ttbdis.2020.101452 http://hdl.handle.net/11449/198776 |
Resumo: | The objective of this study was to detect natural Theileria equi infection in captive tapirs (Tapirus terrestris) in the Brazilian Amazon. Samples from 19 captive tapirs were collected from zoological and botanical gardens and conservation parks in the Pará (n = 18) and Amazonas (n = 1) states. Whole-blood samples were collected for subsequent screening of T. equi DNA by PCR using the BEC-UF2 and EQUI-R primer set. Microscopic analyses of blood smears revealed T. equi trophozoites in 37% (7/19) of the animals examined, and T. equi DNA was detected in 58% (11/19) of the blood samples analyzed. Sequencing of amplified PCR products revealed an identity with T. equi isolates obtained from horses and waterbuck available in GenBank. In conclusion, T. equi infection occurs in captive tapirs in the Brazilian Amazon, and these mammals could potentially act as reservoirs. |
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Natural Theileria equi infection in captive Tapirus terrestris (Perissodactyla: Tapiridae) in the Brazilian AmazonBrazilTapirus terrestrisTheileria equiThe objective of this study was to detect natural Theileria equi infection in captive tapirs (Tapirus terrestris) in the Brazilian Amazon. Samples from 19 captive tapirs were collected from zoological and botanical gardens and conservation parks in the Pará (n = 18) and Amazonas (n = 1) states. Whole-blood samples were collected for subsequent screening of T. equi DNA by PCR using the BEC-UF2 and EQUI-R primer set. Microscopic analyses of blood smears revealed T. equi trophozoites in 37% (7/19) of the animals examined, and T. equi DNA was detected in 58% (11/19) of the blood samples analyzed. Sequencing of amplified PCR products revealed an identity with T. equi isolates obtained from horses and waterbuck available in GenBank. In conclusion, T. equi infection occurs in captive tapirs in the Brazilian Amazon, and these mammals could potentially act as reservoirs.Laboratory of Animal Parasitology Institute of Veterinary Medicine Federal University of Pará (UFPA)Department of Veterinary Clinic and Surgery School of Agricultural and Veterinary Sciences São Paulo State University (UNESP)Department of Veterinary Clinic School of Veterinary Medicine and Zootechnics Federal University of Tocantins (UFT)Department of Veterinary Clinic and Surgery School of Agricultural and Veterinary Sciences São Paulo State University (UNESP)Universidade Federal do Pará (UFPA)Universidade Estadual Paulista (Unesp)Federal University of Tocantins (UFT)de Souza Gonçalves, Thamirysde Nazaré Leite Barros, FláviaInoue, Larissa Saoride Farias, Diana Mariados Santos Lima, JaquelineNobre, Adriana VasconcelosAzenha Aidar, Eveline Simões [UNESP]Ferreira Diniz, Rafael RomeuGering, Ana PaulaScofield, Alessandra2020-12-12T01:21:43Z2020-12-12T01:21:43Z2020-07-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.ttbdis.2020.101452Ticks and Tick-borne Diseases, v. 11, n. 4, 2020.1877-96031877-959Xhttp://hdl.handle.net/11449/19877610.1016/j.ttbdis.2020.1014522-s2.0-85083896344Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengTicks and Tick-borne Diseasesinfo:eu-repo/semantics/openAccess2021-10-22T20:18:29Zoai:repositorio.unesp.br:11449/198776Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T14:07:55.741969Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
| dc.title.none.fl_str_mv |
Natural Theileria equi infection in captive Tapirus terrestris (Perissodactyla: Tapiridae) in the Brazilian Amazon |
| title |
Natural Theileria equi infection in captive Tapirus terrestris (Perissodactyla: Tapiridae) in the Brazilian Amazon |
| spellingShingle |
Natural Theileria equi infection in captive Tapirus terrestris (Perissodactyla: Tapiridae) in the Brazilian Amazon de Souza Gonçalves, Thamirys Brazil Tapirus terrestris Theileria equi |
| title_short |
Natural Theileria equi infection in captive Tapirus terrestris (Perissodactyla: Tapiridae) in the Brazilian Amazon |
| title_full |
Natural Theileria equi infection in captive Tapirus terrestris (Perissodactyla: Tapiridae) in the Brazilian Amazon |
| title_fullStr |
Natural Theileria equi infection in captive Tapirus terrestris (Perissodactyla: Tapiridae) in the Brazilian Amazon |
| title_full_unstemmed |
Natural Theileria equi infection in captive Tapirus terrestris (Perissodactyla: Tapiridae) in the Brazilian Amazon |
| title_sort |
Natural Theileria equi infection in captive Tapirus terrestris (Perissodactyla: Tapiridae) in the Brazilian Amazon |
| author |
de Souza Gonçalves, Thamirys |
| author_facet |
de Souza Gonçalves, Thamirys de Nazaré Leite Barros, Flávia Inoue, Larissa Saori de Farias, Diana Maria dos Santos Lima, Jaqueline Nobre, Adriana Vasconcelos Azenha Aidar, Eveline Simões [UNESP] Ferreira Diniz, Rafael Romeu Gering, Ana Paula Scofield, Alessandra |
| author_role |
author |
| author2 |
de Nazaré Leite Barros, Flávia Inoue, Larissa Saori de Farias, Diana Maria dos Santos Lima, Jaqueline Nobre, Adriana Vasconcelos Azenha Aidar, Eveline Simões [UNESP] Ferreira Diniz, Rafael Romeu Gering, Ana Paula Scofield, Alessandra |
| author2_role |
author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Universidade Federal do Pará (UFPA) Universidade Estadual Paulista (Unesp) Federal University of Tocantins (UFT) |
| dc.contributor.author.fl_str_mv |
de Souza Gonçalves, Thamirys de Nazaré Leite Barros, Flávia Inoue, Larissa Saori de Farias, Diana Maria dos Santos Lima, Jaqueline Nobre, Adriana Vasconcelos Azenha Aidar, Eveline Simões [UNESP] Ferreira Diniz, Rafael Romeu Gering, Ana Paula Scofield, Alessandra |
| dc.subject.por.fl_str_mv |
Brazil Tapirus terrestris Theileria equi |
| topic |
Brazil Tapirus terrestris Theileria equi |
| description |
The objective of this study was to detect natural Theileria equi infection in captive tapirs (Tapirus terrestris) in the Brazilian Amazon. Samples from 19 captive tapirs were collected from zoological and botanical gardens and conservation parks in the Pará (n = 18) and Amazonas (n = 1) states. Whole-blood samples were collected for subsequent screening of T. equi DNA by PCR using the BEC-UF2 and EQUI-R primer set. Microscopic analyses of blood smears revealed T. equi trophozoites in 37% (7/19) of the animals examined, and T. equi DNA was detected in 58% (11/19) of the blood samples analyzed. Sequencing of amplified PCR products revealed an identity with T. equi isolates obtained from horses and waterbuck available in GenBank. In conclusion, T. equi infection occurs in captive tapirs in the Brazilian Amazon, and these mammals could potentially act as reservoirs. |
| publishDate |
2020 |
| dc.date.none.fl_str_mv |
2020-12-12T01:21:43Z 2020-12-12T01:21:43Z 2020-07-01 |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.ttbdis.2020.101452 Ticks and Tick-borne Diseases, v. 11, n. 4, 2020. 1877-9603 1877-959X http://hdl.handle.net/11449/198776 10.1016/j.ttbdis.2020.101452 2-s2.0-85083896344 |
| url |
http://dx.doi.org/10.1016/j.ttbdis.2020.101452 http://hdl.handle.net/11449/198776 |
| identifier_str_mv |
Ticks and Tick-borne Diseases, v. 11, n. 4, 2020. 1877-9603 1877-959X 10.1016/j.ttbdis.2020.101452 2-s2.0-85083896344 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
Ticks and Tick-borne Diseases |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
| instname_str |
Universidade Estadual Paulista (UNESP) |
| instacron_str |
UNESP |
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UNESP |
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Repositório Institucional da UNESP |
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Repositório Institucional da UNESP |
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Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
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