Effects of calcium-phosphate, laser and adhesive on dentin permeability and bond strength

Detalhes bibliográficos
Autor(a) principal: Benitez Sellan, Pablo Lenin [UNESP]
Data de Publicação: 2020
Outros Autores: Zanatta, Rayssa Ferreira, Gomes Torres, Carlos Rocha [UNESP], Tian, Fu-cong, Bergeron, Brian E., Niu, Li-na, Pucci, Cesar Rogério [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.heliyon.2020.e03925
http://hdl.handle.net/11449/200373
Resumo: Objectives: The present study examined a technique for reducing dentin permeability through the application of a calcium phosphate (CaP)-based desensitiser with a laser-assisted process and evaluated adhesive-dentin bond strength. Methods: Thirty dentin discs were divided into two groups according to whether the selected desensitiser (TeethMate; Kuraray Noritake) was used prior to dentin bonding. Each group was subdivided into three subgroups (n = 5): A- Adhesive (Single Bond Universal, 3M ESPE), AL- Adhesive + Laser (Nd:YAG 60 mJ) and LAL- Laser + Adhesive + Laser. Dentin permeability values (%) were recorded before and after desensitiser application. Resin composites were placed over the bonded specimens; the latter were aged prior to microtensile bond strength evaluation. Gelatinolytic activity within the hybrid layers was examined with in-situ zymography using confocal laser scanning microscopy. Data were analysed with ANOVA and Tukey test (α = 0.05). Results: Significant differences in dentin permeability were identified for all groups (p = 0.00). Both laser treatment (p = 0.182) and desensitiser application (p = 0.687) did not significantly improve dentin bond strength. Ultrastructure of the resin-dentin interface identified presence of calcium phosphate within dentinal tubules. Laser treatment did not affect hybrid layer ultrastructure. Both treatment modalities (intratubular CaP occlusion and laser) had no influence on gelatinolytic activity within hybrid layers. Conclusion: Although intratubular CaP occlusion and laser treatment were effective in reducing dentin permeability, they did not affect bond strength, interfacial ultrastructure and gelatinolytic activity within hybrid layers. Clinical relevance: Treatment of etched dentin with Nd:YAG Laser at 60 mJ does not adversely affect collagen ultrastructure and gelatinolytic activity within the hybrid layer. The application of a calcium phosphate-based desensitiser to etch dentin does not affect dentin bond strength.
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spelling Effects of calcium-phosphate, laser and adhesive on dentin permeability and bond strengthBiomedical engineeringBond strengthCalcium phosphateDentin permeabilityMaterials scienceNd:YAG laserObjectives: The present study examined a technique for reducing dentin permeability through the application of a calcium phosphate (CaP)-based desensitiser with a laser-assisted process and evaluated adhesive-dentin bond strength. Methods: Thirty dentin discs were divided into two groups according to whether the selected desensitiser (TeethMate; Kuraray Noritake) was used prior to dentin bonding. Each group was subdivided into three subgroups (n = 5): A- Adhesive (Single Bond Universal, 3M ESPE), AL- Adhesive + Laser (Nd:YAG 60 mJ) and LAL- Laser + Adhesive + Laser. Dentin permeability values (%) were recorded before and after desensitiser application. Resin composites were placed over the bonded specimens; the latter were aged prior to microtensile bond strength evaluation. Gelatinolytic activity within the hybrid layers was examined with in-situ zymography using confocal laser scanning microscopy. Data were analysed with ANOVA and Tukey test (α = 0.05). Results: Significant differences in dentin permeability were identified for all groups (p = 0.00). Both laser treatment (p = 0.182) and desensitiser application (p = 0.687) did not significantly improve dentin bond strength. Ultrastructure of the resin-dentin interface identified presence of calcium phosphate within dentinal tubules. Laser treatment did not affect hybrid layer ultrastructure. Both treatment modalities (intratubular CaP occlusion and laser) had no influence on gelatinolytic activity within hybrid layers. Conclusion: Although intratubular CaP occlusion and laser treatment were effective in reducing dentin permeability, they did not affect bond strength, interfacial ultrastructure and gelatinolytic activity within hybrid layers. Clinical relevance: Treatment of etched dentin with Nd:YAG Laser at 60 mJ does not adversely affect collagen ultrastructure and gelatinolytic activity within the hybrid layer. The application of a calcium phosphate-based desensitiser to etch dentin does not affect dentin bond strength.Materials science; Biomedical engineering; Bond strength; Calcium phosphate; Dentin permeability; Nd:YAG laser.Department of Restorative Dentistry Institute of Science and Technology São Paulo State University-UNESP, São Jose dos CamposDepartment of Restorative Dentistry School of Dentistry University of TaubatéDepartment of Endodontics The Dental College of Georgia Augusta UniversityState Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi Key Laboratory of Stomatology Department of Prosthodontics School of Stomatology The Fourth Military Medical UniversitySchool of Dentistry Universidad Espiritu SantoDepartment of Restorative Dentistry Institute of Science and Technology São Paulo State University-UNESP, São Jose dos CamposUniversidade Estadual Paulista (Unesp)University of TaubatéAugusta UniversityThe Fourth Military Medical UniversityUniversidad Espiritu SantoBenitez Sellan, Pablo Lenin [UNESP]Zanatta, Rayssa FerreiraGomes Torres, Carlos Rocha [UNESP]Tian, Fu-congBergeron, Brian E.Niu, Li-naPucci, Cesar Rogério [UNESP]2020-12-12T02:04:52Z2020-12-12T02:04:52Z2020-05-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.heliyon.2020.e03925Heliyon, v. 6, n. 5, 2020.2405-8440http://hdl.handle.net/11449/20037310.1016/j.heliyon.2020.e039252-s2.0-8508425496117540206528748500000-0003-4830-0400Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengHeliyoninfo:eu-repo/semantics/openAccess2021-10-23T12:31:47Zoai:repositorio.unesp.br:11449/200373Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:07:51.095361Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Effects of calcium-phosphate, laser and adhesive on dentin permeability and bond strength
title Effects of calcium-phosphate, laser and adhesive on dentin permeability and bond strength
spellingShingle Effects of calcium-phosphate, laser and adhesive on dentin permeability and bond strength
Benitez Sellan, Pablo Lenin [UNESP]
Biomedical engineering
Bond strength
Calcium phosphate
Dentin permeability
Materials science
Nd:YAG laser
title_short Effects of calcium-phosphate, laser and adhesive on dentin permeability and bond strength
title_full Effects of calcium-phosphate, laser and adhesive on dentin permeability and bond strength
title_fullStr Effects of calcium-phosphate, laser and adhesive on dentin permeability and bond strength
title_full_unstemmed Effects of calcium-phosphate, laser and adhesive on dentin permeability and bond strength
title_sort Effects of calcium-phosphate, laser and adhesive on dentin permeability and bond strength
author Benitez Sellan, Pablo Lenin [UNESP]
author_facet Benitez Sellan, Pablo Lenin [UNESP]
Zanatta, Rayssa Ferreira
Gomes Torres, Carlos Rocha [UNESP]
Tian, Fu-cong
Bergeron, Brian E.
Niu, Li-na
Pucci, Cesar Rogério [UNESP]
author_role author
author2 Zanatta, Rayssa Ferreira
Gomes Torres, Carlos Rocha [UNESP]
Tian, Fu-cong
Bergeron, Brian E.
Niu, Li-na
Pucci, Cesar Rogério [UNESP]
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
University of Taubaté
Augusta University
The Fourth Military Medical University
Universidad Espiritu Santo
dc.contributor.author.fl_str_mv Benitez Sellan, Pablo Lenin [UNESP]
Zanatta, Rayssa Ferreira
Gomes Torres, Carlos Rocha [UNESP]
Tian, Fu-cong
Bergeron, Brian E.
Niu, Li-na
Pucci, Cesar Rogério [UNESP]
dc.subject.por.fl_str_mv Biomedical engineering
Bond strength
Calcium phosphate
Dentin permeability
Materials science
Nd:YAG laser
topic Biomedical engineering
Bond strength
Calcium phosphate
Dentin permeability
Materials science
Nd:YAG laser
description Objectives: The present study examined a technique for reducing dentin permeability through the application of a calcium phosphate (CaP)-based desensitiser with a laser-assisted process and evaluated adhesive-dentin bond strength. Methods: Thirty dentin discs were divided into two groups according to whether the selected desensitiser (TeethMate; Kuraray Noritake) was used prior to dentin bonding. Each group was subdivided into three subgroups (n = 5): A- Adhesive (Single Bond Universal, 3M ESPE), AL- Adhesive + Laser (Nd:YAG 60 mJ) and LAL- Laser + Adhesive + Laser. Dentin permeability values (%) were recorded before and after desensitiser application. Resin composites were placed over the bonded specimens; the latter were aged prior to microtensile bond strength evaluation. Gelatinolytic activity within the hybrid layers was examined with in-situ zymography using confocal laser scanning microscopy. Data were analysed with ANOVA and Tukey test (α = 0.05). Results: Significant differences in dentin permeability were identified for all groups (p = 0.00). Both laser treatment (p = 0.182) and desensitiser application (p = 0.687) did not significantly improve dentin bond strength. Ultrastructure of the resin-dentin interface identified presence of calcium phosphate within dentinal tubules. Laser treatment did not affect hybrid layer ultrastructure. Both treatment modalities (intratubular CaP occlusion and laser) had no influence on gelatinolytic activity within hybrid layers. Conclusion: Although intratubular CaP occlusion and laser treatment were effective in reducing dentin permeability, they did not affect bond strength, interfacial ultrastructure and gelatinolytic activity within hybrid layers. Clinical relevance: Treatment of etched dentin with Nd:YAG Laser at 60 mJ does not adversely affect collagen ultrastructure and gelatinolytic activity within the hybrid layer. The application of a calcium phosphate-based desensitiser to etch dentin does not affect dentin bond strength.
publishDate 2020
dc.date.none.fl_str_mv 2020-12-12T02:04:52Z
2020-12-12T02:04:52Z
2020-05-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.heliyon.2020.e03925
Heliyon, v. 6, n. 5, 2020.
2405-8440
http://hdl.handle.net/11449/200373
10.1016/j.heliyon.2020.e03925
2-s2.0-85084254961
1754020652874850
0000-0003-4830-0400
url http://dx.doi.org/10.1016/j.heliyon.2020.e03925
http://hdl.handle.net/11449/200373
identifier_str_mv Heliyon, v. 6, n. 5, 2020.
2405-8440
10.1016/j.heliyon.2020.e03925
2-s2.0-85084254961
1754020652874850
0000-0003-4830-0400
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Heliyon
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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