Beta-lactamase detection in Staphylococcus aureus and coagulase-negative Staphylococcus isolated from bovine mastitis

• Autor(a) principal: Robles, Bruno F. [UNESP]
• Data de Publicação: 2014
• Outros Autores: Nóbrega, Diego B. [UNESP], Guimarães, Felipe F. [UNESP], Wanderley, Guido G. [UNESP], Langoni, Hélio [UNESP]
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNESP
• DOI: 10.1590/S0100-736X2014000400004
• Texto Completo: http://dx.doi.org/10.1590/S0100-736X2014000400004; http://hdl.handle.net/11449/109470
• topic: Beta-lactamase; blaZ; Staphylococcus coagulase negativa; Staphylococcus aureus; Beta-lactamase; blaZ; coagulase-negative Staphylococcus; Staphylococcus aureus

The objectives of the study were to evaluate the presence/production of beta-lactamases by both phenotypic and genotypic methods, verify whether results are dependent of bacteria type (Staphylococcus aureus versus coagulase-negative Staphylococcus - CNS) and verify the agreement between tests. A total of 200 bacteria samples from 21 different herds were enrolled, being 100 CNS and 100 S. aureus. Beta-lactamase presence/detection was performed by different tests (PCR, clover leaf test - CLT, Nitrocefin disk, and in vitro resistance to penicillin). Results of all tests were not dependent of bacteria type (CNS or S. aureus). Several S. aureus beta-lactamase producing isolates were from the same herd. Phenotypic tests excluding in vitro resistance to penicillin showed a strong association measured by the kappa coefficient for both bacteria species. Nitrocefin and CLT are more reliable tests for detecting beta-lactamase production in staphylococci.