Selective isolation of agents of chromoblastomycosis from insect-associated environmental sources
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
DOI: | 10.1016/j.funbio.2020.02.002 |
Texto Completo: | http://dx.doi.org/10.1016/j.funbio.2020.02.002 http://hdl.handle.net/11449/200102 |
Resumo: | Chromoblastomycosis is a neglected disease characterized by cutaneous, subcutaneous or disseminated lesions. It is considered an occupational infectious disease that affects mostly rural workers exposed to contaminated soil and vegetal matter. Lesions mostly arise after a traumatic inoculation of herpotrichiellaceous fungi from the Chaetothyriales order. However, the environmental niche of the agents of the disease remains obscure. Its association with insects has been predicted in a few studies. Therefore, the present work aimed to analyze if social insects, specifically ants, bees, and termites, provide a suitable habitat for the fungi concerned. The mineral oil flotation method was used to isolate the microorganisms. Nine isolates were recovered and phylogenetic analysis identified two strains as potential agents of chromoblastomycosis, i.e., Fonsecaea pedrosoi CMRP 3076, obtained from a termite nest (n = 1) and Rhinocladiella similis CMRP 3079 from an ant exoskeleton (n = 1). In addition, we also identified Fonsecaea brasiliensis CMRP 3445 from termites (n = 1), Exophiala xenobiotica CMRP 3077 from ant exoskeleton (n = 1), Cyphellophoraceae CMRP 3103 from bees (n = 1), Cladosporium sp. CMRP 3119 from bees (n = 1), Hawksworthiomyces sp. CMRP 3102 from termites (n = 1), and Cryptendoxyla sp. from termites (n = 2). The environmental isolate of F. pedrosoi CMRP 3076 was tested in two animal models, Tenebrio molitor and Wistar rat, for its pathogenic potential with fungal retention in T. molitor tissue. In the Wistar rat, the cells resembling muriform cells were observed 30 d after inoculation. |
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Selective isolation of agents of chromoblastomycosis from insect-associated environmental sourcesAtta laevigataBlack yeastsFonsecaea pedrosoiMelipona flavolineataNasutitermes sp.Scaptotrigona posticaChromoblastomycosis is a neglected disease characterized by cutaneous, subcutaneous or disseminated lesions. It is considered an occupational infectious disease that affects mostly rural workers exposed to contaminated soil and vegetal matter. Lesions mostly arise after a traumatic inoculation of herpotrichiellaceous fungi from the Chaetothyriales order. However, the environmental niche of the agents of the disease remains obscure. Its association with insects has been predicted in a few studies. Therefore, the present work aimed to analyze if social insects, specifically ants, bees, and termites, provide a suitable habitat for the fungi concerned. The mineral oil flotation method was used to isolate the microorganisms. Nine isolates were recovered and phylogenetic analysis identified two strains as potential agents of chromoblastomycosis, i.e., Fonsecaea pedrosoi CMRP 3076, obtained from a termite nest (n = 1) and Rhinocladiella similis CMRP 3079 from an ant exoskeleton (n = 1). In addition, we also identified Fonsecaea brasiliensis CMRP 3445 from termites (n = 1), Exophiala xenobiotica CMRP 3077 from ant exoskeleton (n = 1), Cyphellophoraceae CMRP 3103 from bees (n = 1), Cladosporium sp. CMRP 3119 from bees (n = 1), Hawksworthiomyces sp. CMRP 3102 from termites (n = 1), and Cryptendoxyla sp. from termites (n = 2). The environmental isolate of F. pedrosoi CMRP 3076 was tested in two animal models, Tenebrio molitor and Wistar rat, for its pathogenic potential with fungal retention in T. molitor tissue. In the Wistar rat, the cells resembling muriform cells were observed 30 d after inoculation.National Council for Scientific ResearchFederal Agency for Scientific OrganizationsConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Graduate Program in Microbiology Parasitology and Pathology Federal University of ParanáUniversity Center Campo RealFederal University of ParanáGraduate Program in Pharmacology UFPRMedical Department Federal University of MaranhãoEmbrapa Amazônia Oriental CPATUWesterdijk Fungal Biodiversity InstituteDepartment of Biochemistry and Microbiology Institute of Biological Sciences UNESP-São Paulo State UniversityDivision of Microbial Resources CPQBA University of CampinasCenter of Expertise in Mycology of Radboud University Medical Center Canisius Wilhelmina HospitalDepartment of Biochemistry and Microbiology Institute of Biological Sciences UNESP-São Paulo State UniversityCNPq: 312811/2018–7CAPES: BrazilCAPES: Finance Code 001, BrasiliaFederal University of ParanáUniversity Center Campo RealUniversidade Federal do Paraná (UFPR)Federal University of MaranhãoEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA)Westerdijk Fungal Biodiversity InstituteUniversidade Estadual Paulista (Unesp)Universidade Estadual de Campinas (UNICAMP)Canisius Wilhelmina HospitalLima, Bruna Jacomel Favoreto de SouzaVoidaleski, Morgana FerreiraGomes, Renata RodriguesFornari, GhenifferSoares, Jade Mariane BarbosaBombassaro, AmandaSchneider, Gabriela XavierSoley, Bruna da Silvade Azevedo, Conceição de Maria Pedrozo e SilvaMenezes, CristianoMoreno, Leandro FerreiraAttili-Angelis, Derlene [UNESP]Klisiowicz, Débora do Rociode Hoog, SybrenVicente, Vânia Aparecida2020-12-12T01:57:45Z2020-12-12T01:57:45Z2020-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article194-204http://dx.doi.org/10.1016/j.funbio.2020.02.002Fungal Biology, v. 124, n. 3-4, p. 194-204, 2020.1878-6146http://hdl.handle.net/11449/20010210.1016/j.funbio.2020.02.0022-s2.0-85079896094Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengFungal Biologyinfo:eu-repo/semantics/openAccess2021-10-23T12:14:55Zoai:repositorio.unesp.br:11449/200102Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:48:57.380940Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Selective isolation of agents of chromoblastomycosis from insect-associated environmental sources |
title |
Selective isolation of agents of chromoblastomycosis from insect-associated environmental sources |
spellingShingle |
Selective isolation of agents of chromoblastomycosis from insect-associated environmental sources Selective isolation of agents of chromoblastomycosis from insect-associated environmental sources Lima, Bruna Jacomel Favoreto de Souza Atta laevigata Black yeasts Fonsecaea pedrosoi Melipona flavolineata Nasutitermes sp. Scaptotrigona postica Lima, Bruna Jacomel Favoreto de Souza Atta laevigata Black yeasts Fonsecaea pedrosoi Melipona flavolineata Nasutitermes sp. Scaptotrigona postica |
title_short |
Selective isolation of agents of chromoblastomycosis from insect-associated environmental sources |
title_full |
Selective isolation of agents of chromoblastomycosis from insect-associated environmental sources |
title_fullStr |
Selective isolation of agents of chromoblastomycosis from insect-associated environmental sources Selective isolation of agents of chromoblastomycosis from insect-associated environmental sources |
title_full_unstemmed |
Selective isolation of agents of chromoblastomycosis from insect-associated environmental sources Selective isolation of agents of chromoblastomycosis from insect-associated environmental sources |
title_sort |
Selective isolation of agents of chromoblastomycosis from insect-associated environmental sources |
author |
Lima, Bruna Jacomel Favoreto de Souza |
author_facet |
Lima, Bruna Jacomel Favoreto de Souza Lima, Bruna Jacomel Favoreto de Souza Voidaleski, Morgana Ferreira Gomes, Renata Rodrigues Fornari, Gheniffer Soares, Jade Mariane Barbosa Bombassaro, Amanda Schneider, Gabriela Xavier Soley, Bruna da Silva de Azevedo, Conceição de Maria Pedrozo e Silva Menezes, Cristiano Moreno, Leandro Ferreira Attili-Angelis, Derlene [UNESP] Klisiowicz, Débora do Rocio de Hoog, Sybren Vicente, Vânia Aparecida Voidaleski, Morgana Ferreira Gomes, Renata Rodrigues Fornari, Gheniffer Soares, Jade Mariane Barbosa Bombassaro, Amanda Schneider, Gabriela Xavier Soley, Bruna da Silva de Azevedo, Conceição de Maria Pedrozo e Silva Menezes, Cristiano Moreno, Leandro Ferreira Attili-Angelis, Derlene [UNESP] Klisiowicz, Débora do Rocio de Hoog, Sybren Vicente, Vânia Aparecida |
author_role |
author |
author2 |
Voidaleski, Morgana Ferreira Gomes, Renata Rodrigues Fornari, Gheniffer Soares, Jade Mariane Barbosa Bombassaro, Amanda Schneider, Gabriela Xavier Soley, Bruna da Silva de Azevedo, Conceição de Maria Pedrozo e Silva Menezes, Cristiano Moreno, Leandro Ferreira Attili-Angelis, Derlene [UNESP] Klisiowicz, Débora do Rocio de Hoog, Sybren Vicente, Vânia Aparecida |
author2_role |
author author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Federal University of Paraná University Center Campo Real Universidade Federal do Paraná (UFPR) Federal University of Maranhão Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) Westerdijk Fungal Biodiversity Institute Universidade Estadual Paulista (Unesp) Universidade Estadual de Campinas (UNICAMP) Canisius Wilhelmina Hospital |
dc.contributor.author.fl_str_mv |
Lima, Bruna Jacomel Favoreto de Souza Voidaleski, Morgana Ferreira Gomes, Renata Rodrigues Fornari, Gheniffer Soares, Jade Mariane Barbosa Bombassaro, Amanda Schneider, Gabriela Xavier Soley, Bruna da Silva de Azevedo, Conceição de Maria Pedrozo e Silva Menezes, Cristiano Moreno, Leandro Ferreira Attili-Angelis, Derlene [UNESP] Klisiowicz, Débora do Rocio de Hoog, Sybren Vicente, Vânia Aparecida |
dc.subject.por.fl_str_mv |
Atta laevigata Black yeasts Fonsecaea pedrosoi Melipona flavolineata Nasutitermes sp. Scaptotrigona postica |
topic |
Atta laevigata Black yeasts Fonsecaea pedrosoi Melipona flavolineata Nasutitermes sp. Scaptotrigona postica |
description |
Chromoblastomycosis is a neglected disease characterized by cutaneous, subcutaneous or disseminated lesions. It is considered an occupational infectious disease that affects mostly rural workers exposed to contaminated soil and vegetal matter. Lesions mostly arise after a traumatic inoculation of herpotrichiellaceous fungi from the Chaetothyriales order. However, the environmental niche of the agents of the disease remains obscure. Its association with insects has been predicted in a few studies. Therefore, the present work aimed to analyze if social insects, specifically ants, bees, and termites, provide a suitable habitat for the fungi concerned. The mineral oil flotation method was used to isolate the microorganisms. Nine isolates were recovered and phylogenetic analysis identified two strains as potential agents of chromoblastomycosis, i.e., Fonsecaea pedrosoi CMRP 3076, obtained from a termite nest (n = 1) and Rhinocladiella similis CMRP 3079 from an ant exoskeleton (n = 1). In addition, we also identified Fonsecaea brasiliensis CMRP 3445 from termites (n = 1), Exophiala xenobiotica CMRP 3077 from ant exoskeleton (n = 1), Cyphellophoraceae CMRP 3103 from bees (n = 1), Cladosporium sp. CMRP 3119 from bees (n = 1), Hawksworthiomyces sp. CMRP 3102 from termites (n = 1), and Cryptendoxyla sp. from termites (n = 2). The environmental isolate of F. pedrosoi CMRP 3076 was tested in two animal models, Tenebrio molitor and Wistar rat, for its pathogenic potential with fungal retention in T. molitor tissue. In the Wistar rat, the cells resembling muriform cells were observed 30 d after inoculation. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T01:57:45Z 2020-12-12T01:57:45Z 2020-03-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.funbio.2020.02.002 Fungal Biology, v. 124, n. 3-4, p. 194-204, 2020. 1878-6146 http://hdl.handle.net/11449/200102 10.1016/j.funbio.2020.02.002 2-s2.0-85079896094 |
url |
http://dx.doi.org/10.1016/j.funbio.2020.02.002 http://hdl.handle.net/11449/200102 |
identifier_str_mv |
Fungal Biology, v. 124, n. 3-4, p. 194-204, 2020. 1878-6146 10.1016/j.funbio.2020.02.002 2-s2.0-85079896094 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Fungal Biology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
194-204 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1822218374576340992 |
dc.identifier.doi.none.fl_str_mv |
10.1016/j.funbio.2020.02.002 |