Selective isolation of agents of chromoblastomycosis from insect-associated environmental sources

Detalhes bibliográficos
Autor(a) principal: Lima, Bruna Jacomel Favoreto de Souza
Data de Publicação: 2020
Outros Autores: Voidaleski, Morgana Ferreira, Gomes, Renata Rodrigues, Fornari, Gheniffer, Soares, Jade Mariane Barbosa, Bombassaro, Amanda, Schneider, Gabriela Xavier, Soley, Bruna da Silva, de Azevedo, Conceição de Maria Pedrozo e Silva, Menezes, Cristiano, Moreno, Leandro Ferreira, Attili-Angelis, Derlene [UNESP], Klisiowicz, Débora do Rocio, de Hoog, Sybren, Vicente, Vânia Aparecida
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
DOI: 10.1016/j.funbio.2020.02.002
Texto Completo: http://dx.doi.org/10.1016/j.funbio.2020.02.002
http://hdl.handle.net/11449/200102
Resumo: Chromoblastomycosis is a neglected disease characterized by cutaneous, subcutaneous or disseminated lesions. It is considered an occupational infectious disease that affects mostly rural workers exposed to contaminated soil and vegetal matter. Lesions mostly arise after a traumatic inoculation of herpotrichiellaceous fungi from the Chaetothyriales order. However, the environmental niche of the agents of the disease remains obscure. Its association with insects has been predicted in a few studies. Therefore, the present work aimed to analyze if social insects, specifically ants, bees, and termites, provide a suitable habitat for the fungi concerned. The mineral oil flotation method was used to isolate the microorganisms. Nine isolates were recovered and phylogenetic analysis identified two strains as potential agents of chromoblastomycosis, i.e., Fonsecaea pedrosoi CMRP 3076, obtained from a termite nest (n = 1) and Rhinocladiella similis CMRP 3079 from an ant exoskeleton (n = 1). In addition, we also identified Fonsecaea brasiliensis CMRP 3445 from termites (n = 1), Exophiala xenobiotica CMRP 3077 from ant exoskeleton (n = 1), Cyphellophoraceae CMRP 3103 from bees (n = 1), Cladosporium sp. CMRP 3119 from bees (n = 1), Hawksworthiomyces sp. CMRP 3102 from termites (n = 1), and Cryptendoxyla sp. from termites (n = 2). The environmental isolate of F. pedrosoi CMRP 3076 was tested in two animal models, Tenebrio molitor and Wistar rat, for its pathogenic potential with fungal retention in T. molitor tissue. In the Wistar rat, the cells resembling muriform cells were observed 30 d after inoculation.
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spelling Selective isolation of agents of chromoblastomycosis from insect-associated environmental sourcesAtta laevigataBlack yeastsFonsecaea pedrosoiMelipona flavolineataNasutitermes sp.Scaptotrigona posticaChromoblastomycosis is a neglected disease characterized by cutaneous, subcutaneous or disseminated lesions. It is considered an occupational infectious disease that affects mostly rural workers exposed to contaminated soil and vegetal matter. Lesions mostly arise after a traumatic inoculation of herpotrichiellaceous fungi from the Chaetothyriales order. However, the environmental niche of the agents of the disease remains obscure. Its association with insects has been predicted in a few studies. Therefore, the present work aimed to analyze if social insects, specifically ants, bees, and termites, provide a suitable habitat for the fungi concerned. The mineral oil flotation method was used to isolate the microorganisms. Nine isolates were recovered and phylogenetic analysis identified two strains as potential agents of chromoblastomycosis, i.e., Fonsecaea pedrosoi CMRP 3076, obtained from a termite nest (n = 1) and Rhinocladiella similis CMRP 3079 from an ant exoskeleton (n = 1). In addition, we also identified Fonsecaea brasiliensis CMRP 3445 from termites (n = 1), Exophiala xenobiotica CMRP 3077 from ant exoskeleton (n = 1), Cyphellophoraceae CMRP 3103 from bees (n = 1), Cladosporium sp. CMRP 3119 from bees (n = 1), Hawksworthiomyces sp. CMRP 3102 from termites (n = 1), and Cryptendoxyla sp. from termites (n = 2). The environmental isolate of F. pedrosoi CMRP 3076 was tested in two animal models, Tenebrio molitor and Wistar rat, for its pathogenic potential with fungal retention in T. molitor tissue. In the Wistar rat, the cells resembling muriform cells were observed 30 d after inoculation.National Council for Scientific ResearchFederal Agency for Scientific OrganizationsConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Graduate Program in Microbiology Parasitology and Pathology Federal University of ParanáUniversity Center Campo RealFederal University of ParanáGraduate Program in Pharmacology UFPRMedical Department Federal University of MaranhãoEmbrapa Amazônia Oriental CPATUWesterdijk Fungal Biodiversity InstituteDepartment of Biochemistry and Microbiology Institute of Biological Sciences UNESP-São Paulo State UniversityDivision of Microbial Resources CPQBA University of CampinasCenter of Expertise in Mycology of Radboud University Medical Center Canisius Wilhelmina HospitalDepartment of Biochemistry and Microbiology Institute of Biological Sciences UNESP-São Paulo State UniversityCNPq: 312811/2018–7CAPES: BrazilCAPES: Finance Code 001, BrasiliaFederal University of ParanáUniversity Center Campo RealUniversidade Federal do Paraná (UFPR)Federal University of MaranhãoEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA)Westerdijk Fungal Biodiversity InstituteUniversidade Estadual Paulista (Unesp)Universidade Estadual de Campinas (UNICAMP)Canisius Wilhelmina HospitalLima, Bruna Jacomel Favoreto de SouzaVoidaleski, Morgana FerreiraGomes, Renata RodriguesFornari, GhenifferSoares, Jade Mariane BarbosaBombassaro, AmandaSchneider, Gabriela XavierSoley, Bruna da Silvade Azevedo, Conceição de Maria Pedrozo e SilvaMenezes, CristianoMoreno, Leandro FerreiraAttili-Angelis, Derlene [UNESP]Klisiowicz, Débora do Rociode Hoog, SybrenVicente, Vânia Aparecida2020-12-12T01:57:45Z2020-12-12T01:57:45Z2020-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article194-204http://dx.doi.org/10.1016/j.funbio.2020.02.002Fungal Biology, v. 124, n. 3-4, p. 194-204, 2020.1878-6146http://hdl.handle.net/11449/20010210.1016/j.funbio.2020.02.0022-s2.0-85079896094Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengFungal Biologyinfo:eu-repo/semantics/openAccess2021-10-23T12:14:55Zoai:repositorio.unesp.br:11449/200102Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:48:57.380940Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Selective isolation of agents of chromoblastomycosis from insect-associated environmental sources
title Selective isolation of agents of chromoblastomycosis from insect-associated environmental sources
spellingShingle Selective isolation of agents of chromoblastomycosis from insect-associated environmental sources
Selective isolation of agents of chromoblastomycosis from insect-associated environmental sources
Lima, Bruna Jacomel Favoreto de Souza
Atta laevigata
Black yeasts
Fonsecaea pedrosoi
Melipona flavolineata
Nasutitermes sp.
Scaptotrigona postica
Lima, Bruna Jacomel Favoreto de Souza
Atta laevigata
Black yeasts
Fonsecaea pedrosoi
Melipona flavolineata
Nasutitermes sp.
Scaptotrigona postica
title_short Selective isolation of agents of chromoblastomycosis from insect-associated environmental sources
title_full Selective isolation of agents of chromoblastomycosis from insect-associated environmental sources
title_fullStr Selective isolation of agents of chromoblastomycosis from insect-associated environmental sources
Selective isolation of agents of chromoblastomycosis from insect-associated environmental sources
title_full_unstemmed Selective isolation of agents of chromoblastomycosis from insect-associated environmental sources
Selective isolation of agents of chromoblastomycosis from insect-associated environmental sources
title_sort Selective isolation of agents of chromoblastomycosis from insect-associated environmental sources
author Lima, Bruna Jacomel Favoreto de Souza
author_facet Lima, Bruna Jacomel Favoreto de Souza
Lima, Bruna Jacomel Favoreto de Souza
Voidaleski, Morgana Ferreira
Gomes, Renata Rodrigues
Fornari, Gheniffer
Soares, Jade Mariane Barbosa
Bombassaro, Amanda
Schneider, Gabriela Xavier
Soley, Bruna da Silva
de Azevedo, Conceição de Maria Pedrozo e Silva
Menezes, Cristiano
Moreno, Leandro Ferreira
Attili-Angelis, Derlene [UNESP]
Klisiowicz, Débora do Rocio
de Hoog, Sybren
Vicente, Vânia Aparecida
Voidaleski, Morgana Ferreira
Gomes, Renata Rodrigues
Fornari, Gheniffer
Soares, Jade Mariane Barbosa
Bombassaro, Amanda
Schneider, Gabriela Xavier
Soley, Bruna da Silva
de Azevedo, Conceição de Maria Pedrozo e Silva
Menezes, Cristiano
Moreno, Leandro Ferreira
Attili-Angelis, Derlene [UNESP]
Klisiowicz, Débora do Rocio
de Hoog, Sybren
Vicente, Vânia Aparecida
author_role author
author2 Voidaleski, Morgana Ferreira
Gomes, Renata Rodrigues
Fornari, Gheniffer
Soares, Jade Mariane Barbosa
Bombassaro, Amanda
Schneider, Gabriela Xavier
Soley, Bruna da Silva
de Azevedo, Conceição de Maria Pedrozo e Silva
Menezes, Cristiano
Moreno, Leandro Ferreira
Attili-Angelis, Derlene [UNESP]
Klisiowicz, Débora do Rocio
de Hoog, Sybren
Vicente, Vânia Aparecida
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Federal University of Paraná
University Center Campo Real
Universidade Federal do Paraná (UFPR)
Federal University of Maranhão
Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
Westerdijk Fungal Biodiversity Institute
Universidade Estadual Paulista (Unesp)
Universidade Estadual de Campinas (UNICAMP)
Canisius Wilhelmina Hospital
dc.contributor.author.fl_str_mv Lima, Bruna Jacomel Favoreto de Souza
Voidaleski, Morgana Ferreira
Gomes, Renata Rodrigues
Fornari, Gheniffer
Soares, Jade Mariane Barbosa
Bombassaro, Amanda
Schneider, Gabriela Xavier
Soley, Bruna da Silva
de Azevedo, Conceição de Maria Pedrozo e Silva
Menezes, Cristiano
Moreno, Leandro Ferreira
Attili-Angelis, Derlene [UNESP]
Klisiowicz, Débora do Rocio
de Hoog, Sybren
Vicente, Vânia Aparecida
dc.subject.por.fl_str_mv Atta laevigata
Black yeasts
Fonsecaea pedrosoi
Melipona flavolineata
Nasutitermes sp.
Scaptotrigona postica
topic Atta laevigata
Black yeasts
Fonsecaea pedrosoi
Melipona flavolineata
Nasutitermes sp.
Scaptotrigona postica
description Chromoblastomycosis is a neglected disease characterized by cutaneous, subcutaneous or disseminated lesions. It is considered an occupational infectious disease that affects mostly rural workers exposed to contaminated soil and vegetal matter. Lesions mostly arise after a traumatic inoculation of herpotrichiellaceous fungi from the Chaetothyriales order. However, the environmental niche of the agents of the disease remains obscure. Its association with insects has been predicted in a few studies. Therefore, the present work aimed to analyze if social insects, specifically ants, bees, and termites, provide a suitable habitat for the fungi concerned. The mineral oil flotation method was used to isolate the microorganisms. Nine isolates were recovered and phylogenetic analysis identified two strains as potential agents of chromoblastomycosis, i.e., Fonsecaea pedrosoi CMRP 3076, obtained from a termite nest (n = 1) and Rhinocladiella similis CMRP 3079 from an ant exoskeleton (n = 1). In addition, we also identified Fonsecaea brasiliensis CMRP 3445 from termites (n = 1), Exophiala xenobiotica CMRP 3077 from ant exoskeleton (n = 1), Cyphellophoraceae CMRP 3103 from bees (n = 1), Cladosporium sp. CMRP 3119 from bees (n = 1), Hawksworthiomyces sp. CMRP 3102 from termites (n = 1), and Cryptendoxyla sp. from termites (n = 2). The environmental isolate of F. pedrosoi CMRP 3076 was tested in two animal models, Tenebrio molitor and Wistar rat, for its pathogenic potential with fungal retention in T. molitor tissue. In the Wistar rat, the cells resembling muriform cells were observed 30 d after inoculation.
publishDate 2020
dc.date.none.fl_str_mv 2020-12-12T01:57:45Z
2020-12-12T01:57:45Z
2020-03-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.funbio.2020.02.002
Fungal Biology, v. 124, n. 3-4, p. 194-204, 2020.
1878-6146
http://hdl.handle.net/11449/200102
10.1016/j.funbio.2020.02.002
2-s2.0-85079896094
url http://dx.doi.org/10.1016/j.funbio.2020.02.002
http://hdl.handle.net/11449/200102
identifier_str_mv Fungal Biology, v. 124, n. 3-4, p. 194-204, 2020.
1878-6146
10.1016/j.funbio.2020.02.002
2-s2.0-85079896094
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Fungal Biology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 194-204
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
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dc.identifier.doi.none.fl_str_mv 10.1016/j.funbio.2020.02.002