Validação de método de análise quantitativa para a triancinolona a partir de complexo ternário por espectrofotometria de UV-Vis
Autor(a) principal: | |
---|---|
Data de Publicação: | 2011 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng por |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://serv-bib.fcfar.unesp.br/seer/index.php/Cien_Farm/article/view/1366 http://hdl.handle.net/11449/72500 |
Resumo: | Triamcinolone (TRI), a drug widely used in the treatment of ocular inflammatory diseases, is practically insoluble in water, which limits its use in eye drops. Cyclodextrins (CDs) have been used to increase the solubility or dissolution rate of drugs. The purpose of the present study was to validate a UV-Vis spectrophotometric method for quantitative analysis of TRI in inclusion complexes with beta-cyclodextrin (B-CD) associated with triethanolamine (TEA) (ternary complex). The proposed analytical method was validated with respect to the parameters established by the Brazilian regulatory National Agency of Sanitary Monitoring (ANVISA). The analytical measurements of absorbance were made at 242nm, at room temperature, in a 1-cm path-length cuvette. The precision and accuracy studies were performed at five concentration levels (4, 8, 12, 18 and 20μg.mL -1). The B-CD associated with TEA did not provoke any alteration in the photochemical behavior of TRI. The results for the measured analytical parameters showed the success of the method. The standard curve was linear (r2 > 0.999) in the concentration range from 2 to 24 μg.mL -1. The method achieved good precision levels in the inter-day (relative standard deviation-RSD <3.4%) and reproducibility (RSD <3.8%) tests. The accuracy was about 80% and the pH changes introduced in the robustness study did not reveal any relevant interference at any of the studied concentrations. The experimental results demonstrate a simple, rapid and affordable UV-Vis spectrophotometric method that could be applied to the quantitation of TRI in this ternary complex. |
id |
UNSP_1cdfcce20f819380ebe5766aee7c44f0 |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/72500 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Validação de método de análise quantitativa para a triancinolona a partir de complexo ternário por espectrofotometria de UV-VisValidation of quantitative analysis method for triamcinolone in ternary complexes by UV-Vis spectrophotometryBeta-cyclodextrinTernary complexesTriamcinoloneUV- Vis spectrophotometryValidationbeta cyclodextrinternary complex factortriamcinolonetriethanolamineaccuracyanalytic methodpHphotochemistryquantitative analysisroom temperatureultraviolet spectrophotometryvalidation studyTriamcinolone (TRI), a drug widely used in the treatment of ocular inflammatory diseases, is practically insoluble in water, which limits its use in eye drops. Cyclodextrins (CDs) have been used to increase the solubility or dissolution rate of drugs. The purpose of the present study was to validate a UV-Vis spectrophotometric method for quantitative analysis of TRI in inclusion complexes with beta-cyclodextrin (B-CD) associated with triethanolamine (TEA) (ternary complex). The proposed analytical method was validated with respect to the parameters established by the Brazilian regulatory National Agency of Sanitary Monitoring (ANVISA). The analytical measurements of absorbance were made at 242nm, at room temperature, in a 1-cm path-length cuvette. The precision and accuracy studies were performed at five concentration levels (4, 8, 12, 18 and 20μg.mL -1). The B-CD associated with TEA did not provoke any alteration in the photochemical behavior of TRI. The results for the measured analytical parameters showed the success of the method. The standard curve was linear (r2 > 0.999) in the concentration range from 2 to 24 μg.mL -1. The method achieved good precision levels in the inter-day (relative standard deviation-RSD <3.4%) and reproducibility (RSD <3.8%) tests. The accuracy was about 80% and the pH changes introduced in the robustness study did not reveal any relevant interference at any of the studied concentrations. The experimental results demonstrate a simple, rapid and affordable UV-Vis spectrophotometric method that could be applied to the quantitation of TRI in this ternary complex.Laboratório de Tecnologia e Biotecnologia Farmacêutica TecBioFar Departamento de Farmácia UFRN, Av. Cel. Gustavo Cordeiro de Farias, SN, Natal, RN, CEP 59180-010Ciências Farmacêuticas UFRN, Natal, RNCiências Farmacêuticas Faculdade de Ciências Farmacêuticas Universidade Estadual Paulista-Unesp, Araraquara, SPCiências Farmacêuticas Faculdade de Ciências Farmacêuticas Universidade Estadual Paulista-Unesp, Araraquara, SPTecBioFarUniversidade Federal do Rio Grande do Norte (UFRN)Universidade Estadual Paulista (Unesp)Aquino, G. D AStopilha, R. T.Pedrosa, M. F FSantos, K. S C REgito, E. S TOliveira, Anselmo Gomes de [UNESP]Silva-Junior, A. A.2014-05-27T11:25:55Z2014-05-27T11:25:55Z2011-06-28info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article35-40application/pdfhttp://serv-bib.fcfar.unesp.br/seer/index.php/Cien_Farm/article/view/1366Revista de Ciencias Farmaceuticas Basica e Aplicada, v. 32, n. 1, p. 35-40, 2011.1808-4532http://hdl.handle.net/11449/725002-s2.0-799595324372-s2.0-79959532437.pdf9114495952533044Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengporRevista de Ciências Farmacêuticas Básica e Aplicada0,131info:eu-repo/semantics/openAccess2024-06-24T13:46:34Zoai:repositorio.unesp.br:11449/72500Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-06T00:01:24.190343Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Validação de método de análise quantitativa para a triancinolona a partir de complexo ternário por espectrofotometria de UV-Vis Validation of quantitative analysis method for triamcinolone in ternary complexes by UV-Vis spectrophotometry |
title |
Validação de método de análise quantitativa para a triancinolona a partir de complexo ternário por espectrofotometria de UV-Vis |
spellingShingle |
Validação de método de análise quantitativa para a triancinolona a partir de complexo ternário por espectrofotometria de UV-Vis Aquino, G. D A Beta-cyclodextrin Ternary complexes Triamcinolone UV- Vis spectrophotometry Validation beta cyclodextrin ternary complex factor triamcinolone triethanolamine accuracy analytic method pH photochemistry quantitative analysis room temperature ultraviolet spectrophotometry validation study |
title_short |
Validação de método de análise quantitativa para a triancinolona a partir de complexo ternário por espectrofotometria de UV-Vis |
title_full |
Validação de método de análise quantitativa para a triancinolona a partir de complexo ternário por espectrofotometria de UV-Vis |
title_fullStr |
Validação de método de análise quantitativa para a triancinolona a partir de complexo ternário por espectrofotometria de UV-Vis |
title_full_unstemmed |
Validação de método de análise quantitativa para a triancinolona a partir de complexo ternário por espectrofotometria de UV-Vis |
title_sort |
Validação de método de análise quantitativa para a triancinolona a partir de complexo ternário por espectrofotometria de UV-Vis |
author |
Aquino, G. D A |
author_facet |
Aquino, G. D A Stopilha, R. T. Pedrosa, M. F F Santos, K. S C R Egito, E. S T Oliveira, Anselmo Gomes de [UNESP] Silva-Junior, A. A. |
author_role |
author |
author2 |
Stopilha, R. T. Pedrosa, M. F F Santos, K. S C R Egito, E. S T Oliveira, Anselmo Gomes de [UNESP] Silva-Junior, A. A. |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
TecBioFar Universidade Federal do Rio Grande do Norte (UFRN) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Aquino, G. D A Stopilha, R. T. Pedrosa, M. F F Santos, K. S C R Egito, E. S T Oliveira, Anselmo Gomes de [UNESP] Silva-Junior, A. A. |
dc.subject.por.fl_str_mv |
Beta-cyclodextrin Ternary complexes Triamcinolone UV- Vis spectrophotometry Validation beta cyclodextrin ternary complex factor triamcinolone triethanolamine accuracy analytic method pH photochemistry quantitative analysis room temperature ultraviolet spectrophotometry validation study |
topic |
Beta-cyclodextrin Ternary complexes Triamcinolone UV- Vis spectrophotometry Validation beta cyclodextrin ternary complex factor triamcinolone triethanolamine accuracy analytic method pH photochemistry quantitative analysis room temperature ultraviolet spectrophotometry validation study |
description |
Triamcinolone (TRI), a drug widely used in the treatment of ocular inflammatory diseases, is practically insoluble in water, which limits its use in eye drops. Cyclodextrins (CDs) have been used to increase the solubility or dissolution rate of drugs. The purpose of the present study was to validate a UV-Vis spectrophotometric method for quantitative analysis of TRI in inclusion complexes with beta-cyclodextrin (B-CD) associated with triethanolamine (TEA) (ternary complex). The proposed analytical method was validated with respect to the parameters established by the Brazilian regulatory National Agency of Sanitary Monitoring (ANVISA). The analytical measurements of absorbance were made at 242nm, at room temperature, in a 1-cm path-length cuvette. The precision and accuracy studies were performed at five concentration levels (4, 8, 12, 18 and 20μg.mL -1). The B-CD associated with TEA did not provoke any alteration in the photochemical behavior of TRI. The results for the measured analytical parameters showed the success of the method. The standard curve was linear (r2 > 0.999) in the concentration range from 2 to 24 μg.mL -1. The method achieved good precision levels in the inter-day (relative standard deviation-RSD <3.4%) and reproducibility (RSD <3.8%) tests. The accuracy was about 80% and the pH changes introduced in the robustness study did not reveal any relevant interference at any of the studied concentrations. The experimental results demonstrate a simple, rapid and affordable UV-Vis spectrophotometric method that could be applied to the quantitation of TRI in this ternary complex. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-06-28 2014-05-27T11:25:55Z 2014-05-27T11:25:55Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://serv-bib.fcfar.unesp.br/seer/index.php/Cien_Farm/article/view/1366 Revista de Ciencias Farmaceuticas Basica e Aplicada, v. 32, n. 1, p. 35-40, 2011. 1808-4532 http://hdl.handle.net/11449/72500 2-s2.0-79959532437 2-s2.0-79959532437.pdf 9114495952533044 |
url |
http://serv-bib.fcfar.unesp.br/seer/index.php/Cien_Farm/article/view/1366 http://hdl.handle.net/11449/72500 |
identifier_str_mv |
Revista de Ciencias Farmaceuticas Basica e Aplicada, v. 32, n. 1, p. 35-40, 2011. 1808-4532 2-s2.0-79959532437 2-s2.0-79959532437.pdf 9114495952533044 |
dc.language.iso.fl_str_mv |
eng por |
language |
eng por |
dc.relation.none.fl_str_mv |
Revista de Ciências Farmacêuticas Básica e Aplicada 0,131 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
35-40 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129573549768704 |