Validação de método de análise quantitativa para a triancinolona a partir de complexo ternário por espectrofotometria de UV-Vis

Detalhes bibliográficos
Autor(a) principal: Aquino, G. D A
Data de Publicação: 2011
Outros Autores: Stopilha, R. T., Pedrosa, M. F F, Santos, K. S C R, Egito, E. S T, Oliveira, Anselmo Gomes de [UNESP], Silva-Junior, A. A.
Tipo de documento: Artigo
Idioma: eng
por
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://serv-bib.fcfar.unesp.br/seer/index.php/Cien_Farm/article/view/1366
http://hdl.handle.net/11449/72500
Resumo: Triamcinolone (TRI), a drug widely used in the treatment of ocular inflammatory diseases, is practically insoluble in water, which limits its use in eye drops. Cyclodextrins (CDs) have been used to increase the solubility or dissolution rate of drugs. The purpose of the present study was to validate a UV-Vis spectrophotometric method for quantitative analysis of TRI in inclusion complexes with beta-cyclodextrin (B-CD) associated with triethanolamine (TEA) (ternary complex). The proposed analytical method was validated with respect to the parameters established by the Brazilian regulatory National Agency of Sanitary Monitoring (ANVISA). The analytical measurements of absorbance were made at 242nm, at room temperature, in a 1-cm path-length cuvette. The precision and accuracy studies were performed at five concentration levels (4, 8, 12, 18 and 20μg.mL -1). The B-CD associated with TEA did not provoke any alteration in the photochemical behavior of TRI. The results for the measured analytical parameters showed the success of the method. The standard curve was linear (r2 > 0.999) in the concentration range from 2 to 24 μg.mL -1. The method achieved good precision levels in the inter-day (relative standard deviation-RSD <3.4%) and reproducibility (RSD <3.8%) tests. The accuracy was about 80% and the pH changes introduced in the robustness study did not reveal any relevant interference at any of the studied concentrations. The experimental results demonstrate a simple, rapid and affordable UV-Vis spectrophotometric method that could be applied to the quantitation of TRI in this ternary complex.
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spelling Validação de método de análise quantitativa para a triancinolona a partir de complexo ternário por espectrofotometria de UV-VisValidation of quantitative analysis method for triamcinolone in ternary complexes by UV-Vis spectrophotometryBeta-cyclodextrinTernary complexesTriamcinoloneUV- Vis spectrophotometryValidationbeta cyclodextrinternary complex factortriamcinolonetriethanolamineaccuracyanalytic methodpHphotochemistryquantitative analysisroom temperatureultraviolet spectrophotometryvalidation studyTriamcinolone (TRI), a drug widely used in the treatment of ocular inflammatory diseases, is practically insoluble in water, which limits its use in eye drops. Cyclodextrins (CDs) have been used to increase the solubility or dissolution rate of drugs. The purpose of the present study was to validate a UV-Vis spectrophotometric method for quantitative analysis of TRI in inclusion complexes with beta-cyclodextrin (B-CD) associated with triethanolamine (TEA) (ternary complex). The proposed analytical method was validated with respect to the parameters established by the Brazilian regulatory National Agency of Sanitary Monitoring (ANVISA). The analytical measurements of absorbance were made at 242nm, at room temperature, in a 1-cm path-length cuvette. The precision and accuracy studies were performed at five concentration levels (4, 8, 12, 18 and 20μg.mL -1). The B-CD associated with TEA did not provoke any alteration in the photochemical behavior of TRI. The results for the measured analytical parameters showed the success of the method. The standard curve was linear (r2 > 0.999) in the concentration range from 2 to 24 μg.mL -1. The method achieved good precision levels in the inter-day (relative standard deviation-RSD <3.4%) and reproducibility (RSD <3.8%) tests. The accuracy was about 80% and the pH changes introduced in the robustness study did not reveal any relevant interference at any of the studied concentrations. The experimental results demonstrate a simple, rapid and affordable UV-Vis spectrophotometric method that could be applied to the quantitation of TRI in this ternary complex.Laboratório de Tecnologia e Biotecnologia Farmacêutica TecBioFar Departamento de Farmácia UFRN, Av. Cel. Gustavo Cordeiro de Farias, SN, Natal, RN, CEP 59180-010Ciências Farmacêuticas UFRN, Natal, RNCiências Farmacêuticas Faculdade de Ciências Farmacêuticas Universidade Estadual Paulista-Unesp, Araraquara, SPCiências Farmacêuticas Faculdade de Ciências Farmacêuticas Universidade Estadual Paulista-Unesp, Araraquara, SPTecBioFarUniversidade Federal do Rio Grande do Norte (UFRN)Universidade Estadual Paulista (Unesp)Aquino, G. D AStopilha, R. T.Pedrosa, M. F FSantos, K. S C REgito, E. S TOliveira, Anselmo Gomes de [UNESP]Silva-Junior, A. A.2014-05-27T11:25:55Z2014-05-27T11:25:55Z2011-06-28info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article35-40application/pdfhttp://serv-bib.fcfar.unesp.br/seer/index.php/Cien_Farm/article/view/1366Revista de Ciencias Farmaceuticas Basica e Aplicada, v. 32, n. 1, p. 35-40, 2011.1808-4532http://hdl.handle.net/11449/725002-s2.0-799595324372-s2.0-79959532437.pdf9114495952533044Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengporRevista de Ciências Farmacêuticas Básica e Aplicada0,131info:eu-repo/semantics/openAccess2024-06-24T13:46:34Zoai:repositorio.unesp.br:11449/72500Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-06T00:01:24.190343Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Validação de método de análise quantitativa para a triancinolona a partir de complexo ternário por espectrofotometria de UV-Vis
Validation of quantitative analysis method for triamcinolone in ternary complexes by UV-Vis spectrophotometry
title Validação de método de análise quantitativa para a triancinolona a partir de complexo ternário por espectrofotometria de UV-Vis
spellingShingle Validação de método de análise quantitativa para a triancinolona a partir de complexo ternário por espectrofotometria de UV-Vis
Aquino, G. D A
Beta-cyclodextrin
Ternary complexes
Triamcinolone
UV- Vis spectrophotometry
Validation
beta cyclodextrin
ternary complex factor
triamcinolone
triethanolamine
accuracy
analytic method
pH
photochemistry
quantitative analysis
room temperature
ultraviolet spectrophotometry
validation study
title_short Validação de método de análise quantitativa para a triancinolona a partir de complexo ternário por espectrofotometria de UV-Vis
title_full Validação de método de análise quantitativa para a triancinolona a partir de complexo ternário por espectrofotometria de UV-Vis
title_fullStr Validação de método de análise quantitativa para a triancinolona a partir de complexo ternário por espectrofotometria de UV-Vis
title_full_unstemmed Validação de método de análise quantitativa para a triancinolona a partir de complexo ternário por espectrofotometria de UV-Vis
title_sort Validação de método de análise quantitativa para a triancinolona a partir de complexo ternário por espectrofotometria de UV-Vis
author Aquino, G. D A
author_facet Aquino, G. D A
Stopilha, R. T.
Pedrosa, M. F F
Santos, K. S C R
Egito, E. S T
Oliveira, Anselmo Gomes de [UNESP]
Silva-Junior, A. A.
author_role author
author2 Stopilha, R. T.
Pedrosa, M. F F
Santos, K. S C R
Egito, E. S T
Oliveira, Anselmo Gomes de [UNESP]
Silva-Junior, A. A.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv TecBioFar
Universidade Federal do Rio Grande do Norte (UFRN)
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Aquino, G. D A
Stopilha, R. T.
Pedrosa, M. F F
Santos, K. S C R
Egito, E. S T
Oliveira, Anselmo Gomes de [UNESP]
Silva-Junior, A. A.
dc.subject.por.fl_str_mv Beta-cyclodextrin
Ternary complexes
Triamcinolone
UV- Vis spectrophotometry
Validation
beta cyclodextrin
ternary complex factor
triamcinolone
triethanolamine
accuracy
analytic method
pH
photochemistry
quantitative analysis
room temperature
ultraviolet spectrophotometry
validation study
topic Beta-cyclodextrin
Ternary complexes
Triamcinolone
UV- Vis spectrophotometry
Validation
beta cyclodextrin
ternary complex factor
triamcinolone
triethanolamine
accuracy
analytic method
pH
photochemistry
quantitative analysis
room temperature
ultraviolet spectrophotometry
validation study
description Triamcinolone (TRI), a drug widely used in the treatment of ocular inflammatory diseases, is practically insoluble in water, which limits its use in eye drops. Cyclodextrins (CDs) have been used to increase the solubility or dissolution rate of drugs. The purpose of the present study was to validate a UV-Vis spectrophotometric method for quantitative analysis of TRI in inclusion complexes with beta-cyclodextrin (B-CD) associated with triethanolamine (TEA) (ternary complex). The proposed analytical method was validated with respect to the parameters established by the Brazilian regulatory National Agency of Sanitary Monitoring (ANVISA). The analytical measurements of absorbance were made at 242nm, at room temperature, in a 1-cm path-length cuvette. The precision and accuracy studies were performed at five concentration levels (4, 8, 12, 18 and 20μg.mL -1). The B-CD associated with TEA did not provoke any alteration in the photochemical behavior of TRI. The results for the measured analytical parameters showed the success of the method. The standard curve was linear (r2 > 0.999) in the concentration range from 2 to 24 μg.mL -1. The method achieved good precision levels in the inter-day (relative standard deviation-RSD <3.4%) and reproducibility (RSD <3.8%) tests. The accuracy was about 80% and the pH changes introduced in the robustness study did not reveal any relevant interference at any of the studied concentrations. The experimental results demonstrate a simple, rapid and affordable UV-Vis spectrophotometric method that could be applied to the quantitation of TRI in this ternary complex.
publishDate 2011
dc.date.none.fl_str_mv 2011-06-28
2014-05-27T11:25:55Z
2014-05-27T11:25:55Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://serv-bib.fcfar.unesp.br/seer/index.php/Cien_Farm/article/view/1366
Revista de Ciencias Farmaceuticas Basica e Aplicada, v. 32, n. 1, p. 35-40, 2011.
1808-4532
http://hdl.handle.net/11449/72500
2-s2.0-79959532437
2-s2.0-79959532437.pdf
9114495952533044
url http://serv-bib.fcfar.unesp.br/seer/index.php/Cien_Farm/article/view/1366
http://hdl.handle.net/11449/72500
identifier_str_mv Revista de Ciencias Farmaceuticas Basica e Aplicada, v. 32, n. 1, p. 35-40, 2011.
1808-4532
2-s2.0-79959532437
2-s2.0-79959532437.pdf
9114495952533044
dc.language.iso.fl_str_mv eng
por
language eng
por
dc.relation.none.fl_str_mv Revista de Ciências Farmacêuticas Básica e Aplicada
0,131
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 35-40
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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