Characterization of Gnrh/Gnih elements in the olfacto-retinal system and ovary during zebrafish ovarian maturation
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.mce.2017.04.002 http://hdl.handle.net/11449/174471 |
Resumo: | Gonadotropin releasing hormone (GnRH) is one of the key players of brain-pituitary-gonad axis, exerting overall control over vertebrate reproduction. In zebrafish, two variants were characterized and named as Gnrh2 and Gnrh3. In this species, Gnrh3, the hypohysiotropic form, is expressed by neurons of the olfactory-retinal system, where it is related with food detection, intra/interspecific recognition, visual acuity and retinal processing modulation. Previous studies have reported the presence of Gnrh receptors in the zebrafish retina, but not yet in the zebrafish olfactory epithelium. The current study analyzed the presence of gnrh2 and gnrh3, their receptors (gnrhr 1,2,3 and 4) and gnih (gonadotropin inhibitory hormone) transcripts, as well as the Gnrh3 protein in the olfactory epithelium (OE), olfactory bulb (OB), retina and ovary during zebrafish ovarian maturation. We found an increase of gnrh receptors transcripts in the OE at the final stages of ovarian maturation. In the OE, Gnrh3 protein was detected in the olfactory receptor neurons cilia and in the olfactory nerve fibers. Interestingly, in the OB, we found an inverse expression pattern between gnih and gnrh3. In the retina, gnrhr4 mRNA was found in the nuclei of amacrine, bipolar, and ganglion cells next to Gnrh3 positive fibers. In the ovary, gnrh3, gnrhr2 and gnrhr4 transcripts were found in perinucleolar oocytes, while gnih in oocytes at the cortical alveolus stage. Our results suggested that Gnrh/Gnih elements are involved in the neuromodulation of the sensorial system particularly at the final stages of maturation, playing also a paracrine role in the ovary. |
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Characterization of Gnrh/Gnih elements in the olfacto-retinal system and ovary during zebrafish ovarian maturationGnihGnrhOlfactory bulbOvarian maturationSensorial systemsZebrafishGonadotropin releasing hormone (GnRH) is one of the key players of brain-pituitary-gonad axis, exerting overall control over vertebrate reproduction. In zebrafish, two variants were characterized and named as Gnrh2 and Gnrh3. In this species, Gnrh3, the hypohysiotropic form, is expressed by neurons of the olfactory-retinal system, where it is related with food detection, intra/interspecific recognition, visual acuity and retinal processing modulation. Previous studies have reported the presence of Gnrh receptors in the zebrafish retina, but not yet in the zebrafish olfactory epithelium. The current study analyzed the presence of gnrh2 and gnrh3, their receptors (gnrhr 1,2,3 and 4) and gnih (gonadotropin inhibitory hormone) transcripts, as well as the Gnrh3 protein in the olfactory epithelium (OE), olfactory bulb (OB), retina and ovary during zebrafish ovarian maturation. We found an increase of gnrh receptors transcripts in the OE at the final stages of ovarian maturation. In the OE, Gnrh3 protein was detected in the olfactory receptor neurons cilia and in the olfactory nerve fibers. Interestingly, in the OB, we found an inverse expression pattern between gnih and gnrh3. In the retina, gnrhr4 mRNA was found in the nuclei of amacrine, bipolar, and ganglion cells next to Gnrh3 positive fibers. In the ovary, gnrh3, gnrhr2 and gnrhr4 transcripts were found in perinucleolar oocytes, while gnih in oocytes at the cortical alveolus stage. Our results suggested that Gnrh/Gnih elements are involved in the neuromodulation of the sensorial system particularly at the final stages of maturation, playing also a paracrine role in the ovary.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Aquaculture Center of São Paulo State University (CAUNESP)Department of Animal Morphology and Physiology São Paulo State University (UNESP)Laboratorio de Ictiofisiología y Acuicultura Instituto de Investigaciones Biotecnológicas-Instituto Tecnológico de Chascomús (CONICET-UNSAM)Reproductive and Molecular Biology Group Department of Morphology Institute of Bioscience of Botucatu São Paulo State UniversityAquaculture Center of São Paulo State University (CAUNESP)Department of Animal Morphology and Physiology São Paulo State University (UNESP)Reproductive and Molecular Biology Group Department of Morphology Institute of Bioscience of Botucatu São Paulo State UniversityFAPESP: 2012/00423-6FAPESP: 2014/02481-9Universidade Estadual Paulista (Unesp)Instituto de Investigaciones Biotecnológicas-Instituto Tecnológico de Chascomús (CONICET-UNSAM)Corchuelo, Sheryll [UNESP]Martinez, Emanuel R.M. [UNESP]Butzge, Arno J. [UNESP]Doretto, Lucas B. [UNESP]Ricci, Juliana M.B. [UNESP]Valentin, Fernanda N. [UNESP]Nakaghi, Laura S.O. [UNESP]Somoza, Gustavo M.Nóbrega, Rafael H. [UNESP]2018-12-11T17:11:16Z2018-12-11T17:11:16Z2017-07-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1-13application/pdfhttp://dx.doi.org/10.1016/j.mce.2017.04.002Molecular and Cellular Endocrinology, v. 450, p. 1-13.1872-80570303-7207http://hdl.handle.net/11449/17447110.1016/j.mce.2017.04.0022-s2.0-850175406362-s2.0-85017540636.pdf05157085852539850000-0001-9796-5076Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMolecular and Cellular Endocrinology1,629info:eu-repo/semantics/openAccess2024-04-09T15:43:16Zoai:repositorio.unesp.br:11449/174471Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:54:43.211658Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Characterization of Gnrh/Gnih elements in the olfacto-retinal system and ovary during zebrafish ovarian maturation |
title |
Characterization of Gnrh/Gnih elements in the olfacto-retinal system and ovary during zebrafish ovarian maturation |
spellingShingle |
Characterization of Gnrh/Gnih elements in the olfacto-retinal system and ovary during zebrafish ovarian maturation Corchuelo, Sheryll [UNESP] Gnih Gnrh Olfactory bulb Ovarian maturation Sensorial systems Zebrafish |
title_short |
Characterization of Gnrh/Gnih elements in the olfacto-retinal system and ovary during zebrafish ovarian maturation |
title_full |
Characterization of Gnrh/Gnih elements in the olfacto-retinal system and ovary during zebrafish ovarian maturation |
title_fullStr |
Characterization of Gnrh/Gnih elements in the olfacto-retinal system and ovary during zebrafish ovarian maturation |
title_full_unstemmed |
Characterization of Gnrh/Gnih elements in the olfacto-retinal system and ovary during zebrafish ovarian maturation |
title_sort |
Characterization of Gnrh/Gnih elements in the olfacto-retinal system and ovary during zebrafish ovarian maturation |
author |
Corchuelo, Sheryll [UNESP] |
author_facet |
Corchuelo, Sheryll [UNESP] Martinez, Emanuel R.M. [UNESP] Butzge, Arno J. [UNESP] Doretto, Lucas B. [UNESP] Ricci, Juliana M.B. [UNESP] Valentin, Fernanda N. [UNESP] Nakaghi, Laura S.O. [UNESP] Somoza, Gustavo M. Nóbrega, Rafael H. [UNESP] |
author_role |
author |
author2 |
Martinez, Emanuel R.M. [UNESP] Butzge, Arno J. [UNESP] Doretto, Lucas B. [UNESP] Ricci, Juliana M.B. [UNESP] Valentin, Fernanda N. [UNESP] Nakaghi, Laura S.O. [UNESP] Somoza, Gustavo M. Nóbrega, Rafael H. [UNESP] |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Instituto de Investigaciones Biotecnológicas-Instituto Tecnológico de Chascomús (CONICET-UNSAM) |
dc.contributor.author.fl_str_mv |
Corchuelo, Sheryll [UNESP] Martinez, Emanuel R.M. [UNESP] Butzge, Arno J. [UNESP] Doretto, Lucas B. [UNESP] Ricci, Juliana M.B. [UNESP] Valentin, Fernanda N. [UNESP] Nakaghi, Laura S.O. [UNESP] Somoza, Gustavo M. Nóbrega, Rafael H. [UNESP] |
dc.subject.por.fl_str_mv |
Gnih Gnrh Olfactory bulb Ovarian maturation Sensorial systems Zebrafish |
topic |
Gnih Gnrh Olfactory bulb Ovarian maturation Sensorial systems Zebrafish |
description |
Gonadotropin releasing hormone (GnRH) is one of the key players of brain-pituitary-gonad axis, exerting overall control over vertebrate reproduction. In zebrafish, two variants were characterized and named as Gnrh2 and Gnrh3. In this species, Gnrh3, the hypohysiotropic form, is expressed by neurons of the olfactory-retinal system, where it is related with food detection, intra/interspecific recognition, visual acuity and retinal processing modulation. Previous studies have reported the presence of Gnrh receptors in the zebrafish retina, but not yet in the zebrafish olfactory epithelium. The current study analyzed the presence of gnrh2 and gnrh3, their receptors (gnrhr 1,2,3 and 4) and gnih (gonadotropin inhibitory hormone) transcripts, as well as the Gnrh3 protein in the olfactory epithelium (OE), olfactory bulb (OB), retina and ovary during zebrafish ovarian maturation. We found an increase of gnrh receptors transcripts in the OE at the final stages of ovarian maturation. In the OE, Gnrh3 protein was detected in the olfactory receptor neurons cilia and in the olfactory nerve fibers. Interestingly, in the OB, we found an inverse expression pattern between gnih and gnrh3. In the retina, gnrhr4 mRNA was found in the nuclei of amacrine, bipolar, and ganglion cells next to Gnrh3 positive fibers. In the ovary, gnrh3, gnrhr2 and gnrhr4 transcripts were found in perinucleolar oocytes, while gnih in oocytes at the cortical alveolus stage. Our results suggested that Gnrh/Gnih elements are involved in the neuromodulation of the sensorial system particularly at the final stages of maturation, playing also a paracrine role in the ovary. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-07-15 2018-12-11T17:11:16Z 2018-12-11T17:11:16Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.mce.2017.04.002 Molecular and Cellular Endocrinology, v. 450, p. 1-13. 1872-8057 0303-7207 http://hdl.handle.net/11449/174471 10.1016/j.mce.2017.04.002 2-s2.0-85017540636 2-s2.0-85017540636.pdf 0515708585253985 0000-0001-9796-5076 |
url |
http://dx.doi.org/10.1016/j.mce.2017.04.002 http://hdl.handle.net/11449/174471 |
identifier_str_mv |
Molecular and Cellular Endocrinology, v. 450, p. 1-13. 1872-8057 0303-7207 10.1016/j.mce.2017.04.002 2-s2.0-85017540636 2-s2.0-85017540636.pdf 0515708585253985 0000-0001-9796-5076 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Molecular and Cellular Endocrinology 1,629 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1-13 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808129372692938752 |