Characterization of Gnrh/Gnih elements in the olfacto-retinal system and ovary during zebrafish ovarian maturation

Detalhes bibliográficos
Autor(a) principal: Corchuelo, Sheryll [UNESP]
Data de Publicação: 2017
Outros Autores: Martinez, Emanuel R.M. [UNESP], Butzge, Arno J. [UNESP], Doretto, Lucas B. [UNESP], Ricci, Juliana M.B. [UNESP], Valentin, Fernanda N. [UNESP], Nakaghi, Laura S.O. [UNESP], Somoza, Gustavo M., Nóbrega, Rafael H. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.mce.2017.04.002
http://hdl.handle.net/11449/174471
Resumo: Gonadotropin releasing hormone (GnRH) is one of the key players of brain-pituitary-gonad axis, exerting overall control over vertebrate reproduction. In zebrafish, two variants were characterized and named as Gnrh2 and Gnrh3. In this species, Gnrh3, the hypohysiotropic form, is expressed by neurons of the olfactory-retinal system, where it is related with food detection, intra/interspecific recognition, visual acuity and retinal processing modulation. Previous studies have reported the presence of Gnrh receptors in the zebrafish retina, but not yet in the zebrafish olfactory epithelium. The current study analyzed the presence of gnrh2 and gnrh3, their receptors (gnrhr 1,2,3 and 4) and gnih (gonadotropin inhibitory hormone) transcripts, as well as the Gnrh3 protein in the olfactory epithelium (OE), olfactory bulb (OB), retina and ovary during zebrafish ovarian maturation. We found an increase of gnrh receptors transcripts in the OE at the final stages of ovarian maturation. In the OE, Gnrh3 protein was detected in the olfactory receptor neurons cilia and in the olfactory nerve fibers. Interestingly, in the OB, we found an inverse expression pattern between gnih and gnrh3. In the retina, gnrhr4 mRNA was found in the nuclei of amacrine, bipolar, and ganglion cells next to Gnrh3 positive fibers. In the ovary, gnrh3, gnrhr2 and gnrhr4 transcripts were found in perinucleolar oocytes, while gnih in oocytes at the cortical alveolus stage. Our results suggested that Gnrh/Gnih elements are involved in the neuromodulation of the sensorial system particularly at the final stages of maturation, playing also a paracrine role in the ovary.
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spelling Characterization of Gnrh/Gnih elements in the olfacto-retinal system and ovary during zebrafish ovarian maturationGnihGnrhOlfactory bulbOvarian maturationSensorial systemsZebrafishGonadotropin releasing hormone (GnRH) is one of the key players of brain-pituitary-gonad axis, exerting overall control over vertebrate reproduction. In zebrafish, two variants were characterized and named as Gnrh2 and Gnrh3. In this species, Gnrh3, the hypohysiotropic form, is expressed by neurons of the olfactory-retinal system, where it is related with food detection, intra/interspecific recognition, visual acuity and retinal processing modulation. Previous studies have reported the presence of Gnrh receptors in the zebrafish retina, but not yet in the zebrafish olfactory epithelium. The current study analyzed the presence of gnrh2 and gnrh3, their receptors (gnrhr 1,2,3 and 4) and gnih (gonadotropin inhibitory hormone) transcripts, as well as the Gnrh3 protein in the olfactory epithelium (OE), olfactory bulb (OB), retina and ovary during zebrafish ovarian maturation. We found an increase of gnrh receptors transcripts in the OE at the final stages of ovarian maturation. In the OE, Gnrh3 protein was detected in the olfactory receptor neurons cilia and in the olfactory nerve fibers. Interestingly, in the OB, we found an inverse expression pattern between gnih and gnrh3. In the retina, gnrhr4 mRNA was found in the nuclei of amacrine, bipolar, and ganglion cells next to Gnrh3 positive fibers. In the ovary, gnrh3, gnrhr2 and gnrhr4 transcripts were found in perinucleolar oocytes, while gnih in oocytes at the cortical alveolus stage. Our results suggested that Gnrh/Gnih elements are involved in the neuromodulation of the sensorial system particularly at the final stages of maturation, playing also a paracrine role in the ovary.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Aquaculture Center of São Paulo State University (CAUNESP)Department of Animal Morphology and Physiology São Paulo State University (UNESP)Laboratorio de Ictiofisiología y Acuicultura Instituto de Investigaciones Biotecnológicas-Instituto Tecnológico de Chascomús (CONICET-UNSAM)Reproductive and Molecular Biology Group Department of Morphology Institute of Bioscience of Botucatu São Paulo State UniversityAquaculture Center of São Paulo State University (CAUNESP)Department of Animal Morphology and Physiology São Paulo State University (UNESP)Reproductive and Molecular Biology Group Department of Morphology Institute of Bioscience of Botucatu São Paulo State UniversityFAPESP: 2012/00423-6FAPESP: 2014/02481-9Universidade Estadual Paulista (Unesp)Instituto de Investigaciones Biotecnológicas-Instituto Tecnológico de Chascomús (CONICET-UNSAM)Corchuelo, Sheryll [UNESP]Martinez, Emanuel R.M. [UNESP]Butzge, Arno J. [UNESP]Doretto, Lucas B. [UNESP]Ricci, Juliana M.B. [UNESP]Valentin, Fernanda N. [UNESP]Nakaghi, Laura S.O. [UNESP]Somoza, Gustavo M.Nóbrega, Rafael H. [UNESP]2018-12-11T17:11:16Z2018-12-11T17:11:16Z2017-07-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1-13application/pdfhttp://dx.doi.org/10.1016/j.mce.2017.04.002Molecular and Cellular Endocrinology, v. 450, p. 1-13.1872-80570303-7207http://hdl.handle.net/11449/17447110.1016/j.mce.2017.04.0022-s2.0-850175406362-s2.0-85017540636.pdf05157085852539850000-0001-9796-5076Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMolecular and Cellular Endocrinology1,629info:eu-repo/semantics/openAccess2024-04-09T15:43:16Zoai:repositorio.unesp.br:11449/174471Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:54:43.211658Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Characterization of Gnrh/Gnih elements in the olfacto-retinal system and ovary during zebrafish ovarian maturation
title Characterization of Gnrh/Gnih elements in the olfacto-retinal system and ovary during zebrafish ovarian maturation
spellingShingle Characterization of Gnrh/Gnih elements in the olfacto-retinal system and ovary during zebrafish ovarian maturation
Corchuelo, Sheryll [UNESP]
Gnih
Gnrh
Olfactory bulb
Ovarian maturation
Sensorial systems
Zebrafish
title_short Characterization of Gnrh/Gnih elements in the olfacto-retinal system and ovary during zebrafish ovarian maturation
title_full Characterization of Gnrh/Gnih elements in the olfacto-retinal system and ovary during zebrafish ovarian maturation
title_fullStr Characterization of Gnrh/Gnih elements in the olfacto-retinal system and ovary during zebrafish ovarian maturation
title_full_unstemmed Characterization of Gnrh/Gnih elements in the olfacto-retinal system and ovary during zebrafish ovarian maturation
title_sort Characterization of Gnrh/Gnih elements in the olfacto-retinal system and ovary during zebrafish ovarian maturation
author Corchuelo, Sheryll [UNESP]
author_facet Corchuelo, Sheryll [UNESP]
Martinez, Emanuel R.M. [UNESP]
Butzge, Arno J. [UNESP]
Doretto, Lucas B. [UNESP]
Ricci, Juliana M.B. [UNESP]
Valentin, Fernanda N. [UNESP]
Nakaghi, Laura S.O. [UNESP]
Somoza, Gustavo M.
Nóbrega, Rafael H. [UNESP]
author_role author
author2 Martinez, Emanuel R.M. [UNESP]
Butzge, Arno J. [UNESP]
Doretto, Lucas B. [UNESP]
Ricci, Juliana M.B. [UNESP]
Valentin, Fernanda N. [UNESP]
Nakaghi, Laura S.O. [UNESP]
Somoza, Gustavo M.
Nóbrega, Rafael H. [UNESP]
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Instituto de Investigaciones Biotecnológicas-Instituto Tecnológico de Chascomús (CONICET-UNSAM)
dc.contributor.author.fl_str_mv Corchuelo, Sheryll [UNESP]
Martinez, Emanuel R.M. [UNESP]
Butzge, Arno J. [UNESP]
Doretto, Lucas B. [UNESP]
Ricci, Juliana M.B. [UNESP]
Valentin, Fernanda N. [UNESP]
Nakaghi, Laura S.O. [UNESP]
Somoza, Gustavo M.
Nóbrega, Rafael H. [UNESP]
dc.subject.por.fl_str_mv Gnih
Gnrh
Olfactory bulb
Ovarian maturation
Sensorial systems
Zebrafish
topic Gnih
Gnrh
Olfactory bulb
Ovarian maturation
Sensorial systems
Zebrafish
description Gonadotropin releasing hormone (GnRH) is one of the key players of brain-pituitary-gonad axis, exerting overall control over vertebrate reproduction. In zebrafish, two variants were characterized and named as Gnrh2 and Gnrh3. In this species, Gnrh3, the hypohysiotropic form, is expressed by neurons of the olfactory-retinal system, where it is related with food detection, intra/interspecific recognition, visual acuity and retinal processing modulation. Previous studies have reported the presence of Gnrh receptors in the zebrafish retina, but not yet in the zebrafish olfactory epithelium. The current study analyzed the presence of gnrh2 and gnrh3, their receptors (gnrhr 1,2,3 and 4) and gnih (gonadotropin inhibitory hormone) transcripts, as well as the Gnrh3 protein in the olfactory epithelium (OE), olfactory bulb (OB), retina and ovary during zebrafish ovarian maturation. We found an increase of gnrh receptors transcripts in the OE at the final stages of ovarian maturation. In the OE, Gnrh3 protein was detected in the olfactory receptor neurons cilia and in the olfactory nerve fibers. Interestingly, in the OB, we found an inverse expression pattern between gnih and gnrh3. In the retina, gnrhr4 mRNA was found in the nuclei of amacrine, bipolar, and ganglion cells next to Gnrh3 positive fibers. In the ovary, gnrh3, gnrhr2 and gnrhr4 transcripts were found in perinucleolar oocytes, while gnih in oocytes at the cortical alveolus stage. Our results suggested that Gnrh/Gnih elements are involved in the neuromodulation of the sensorial system particularly at the final stages of maturation, playing also a paracrine role in the ovary.
publishDate 2017
dc.date.none.fl_str_mv 2017-07-15
2018-12-11T17:11:16Z
2018-12-11T17:11:16Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.mce.2017.04.002
Molecular and Cellular Endocrinology, v. 450, p. 1-13.
1872-8057
0303-7207
http://hdl.handle.net/11449/174471
10.1016/j.mce.2017.04.002
2-s2.0-85017540636
2-s2.0-85017540636.pdf
0515708585253985
0000-0001-9796-5076
url http://dx.doi.org/10.1016/j.mce.2017.04.002
http://hdl.handle.net/11449/174471
identifier_str_mv Molecular and Cellular Endocrinology, v. 450, p. 1-13.
1872-8057
0303-7207
10.1016/j.mce.2017.04.002
2-s2.0-85017540636
2-s2.0-85017540636.pdf
0515708585253985
0000-0001-9796-5076
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Molecular and Cellular Endocrinology
1,629
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 1-13
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
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