Chromosomal segments may explain the antibody response cooperation for canine leishmaniasis pathogenesis
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.vetpar.2020.109276 http://hdl.handle.net/11449/209810 |
Resumo: | Visceral leishmaniasis (VL) is marked by hyperactivation of a humoral response secreting high quantity of immunoglobulins (Igs) that are inaccessible to intracellular parasites. Here we investigated the contributions of the antibody response to the canine leishmaniasis pathogenesis. Using correlation and genome-wide association analysis, we investigated the relationship of anti-Leishmania infantum immunoglobulin classes levels with parasite burden, clinical response, renal/hepatic biochemical, and oxidative stress markers in dogs from endemic areas of VL. Immunoglobulin G (IgG) and IgA were positively correlated with parasite burden on lymph node and blood. Increased IgG, IgA and IgE levels were associated with severe canine leishmaniasis (CanL) whereas IgM was elevated in uninfected exposed dogs. Correlations of IgM, IgG and IgA with creatinine, urea, AST and ALT levels in the serum were suggested an involvement of those Igs with renal and hepatic changes. The correlogram of oxidative radicals and antioxidants revealed a likely relationship of IgM, IgG and IgA with oxidative stress and lipid pemxidation in the blood, suggested as mechanisms mediating tissue damage and CanL worsening. The gene mapping on chromosomal segments associated with the quantitative variation of immunoglobulin classes identified genetic signatures involved with reactive oxygen species generation, phagolysosome maturation and rupture, free iron availability, Thl/Th2 differenciation and, immunoglobulin clearance. The findings demonstrated the roles of the antibody response as resistance or susceptibility markers and mediators of CanL pathogenesis. In addition we pinpointed candidate genes as potential targets for the therapy against the damage caused by exacerbated antibody response and parasitism in VL. |
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Chromosomal segments may explain the antibody response cooperation for canine leishmaniasis pathogenesisGWASCanine leishmaniasisAntiboby responseOxidative stressRenal-hepatic changesVisceral leishmaniasis (VL) is marked by hyperactivation of a humoral response secreting high quantity of immunoglobulins (Igs) that are inaccessible to intracellular parasites. Here we investigated the contributions of the antibody response to the canine leishmaniasis pathogenesis. Using correlation and genome-wide association analysis, we investigated the relationship of anti-Leishmania infantum immunoglobulin classes levels with parasite burden, clinical response, renal/hepatic biochemical, and oxidative stress markers in dogs from endemic areas of VL. Immunoglobulin G (IgG) and IgA were positively correlated with parasite burden on lymph node and blood. Increased IgG, IgA and IgE levels were associated with severe canine leishmaniasis (CanL) whereas IgM was elevated in uninfected exposed dogs. Correlations of IgM, IgG and IgA with creatinine, urea, AST and ALT levels in the serum were suggested an involvement of those Igs with renal and hepatic changes. The correlogram of oxidative radicals and antioxidants revealed a likely relationship of IgM, IgG and IgA with oxidative stress and lipid pemxidation in the blood, suggested as mechanisms mediating tissue damage and CanL worsening. The gene mapping on chromosomal segments associated with the quantitative variation of immunoglobulin classes identified genetic signatures involved with reactive oxygen species generation, phagolysosome maturation and rupture, free iron availability, Thl/Th2 differenciation and, immunoglobulin clearance. The findings demonstrated the roles of the antibody response as resistance or susceptibility markers and mediators of CanL pathogenesis. In addition we pinpointed candidate genes as potential targets for the therapy against the damage caused by exacerbated antibody response and parasitism in VL.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Hospital das Clinicas, Faculdade de Medicina da Universidade de Sao Paulo (HC-FMUSP), BrazilUniv Sao Paulo, Fac Med, Lab Patol Doencas Infecciosas, BR-01246903 Sao Paulo, BrazilUniv Estadual Paulista, Fac Ciencias Agr & Vet, Dept Med Vet Prevent & Reprod Anim, BR-14884900 Jaboticabal, SP, BrazilUniv Salvador, Escola Saude, BR-41720200 Salvador, BA, BrazilUniv Estadual Paulista, Fac Med Vet Aracatuba, Dept Apoio Prod & Saude Anim, BR-16015050 Aracatuba, SP, BrazilUniv Estadual Paulista, Fac Med Vet, Dept Clin Cirurgia & Reprod Anim, BR-16015050 Aracatuba, SP, BrazilUniv Sao Paulo, Fac Med Vet & Zootecnia, Dept Clin, BR-05508270 Sao Paulo, BrazilUniv Estadual Paulista, Fac Ciencias Agr & Vet, Dept Med Vet Prevent & Reprod Anim, BR-14884900 Jaboticabal, SP, BrazilUniv Estadual Paulista, Fac Med Vet Aracatuba, Dept Apoio Prod & Saude Anim, BR-16015050 Aracatuba, SP, BrazilUniv Estadual Paulista, Fac Med Vet, Dept Clin Cirurgia & Reprod Anim, BR-16015050 Aracatuba, SP, BrazilFAPESP: 2012/50285-9FAPESP: 2012/05847-9FAPESP: 2014/01095-8CNPq: 476479/2012-6Elsevier B.V.Universidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)Univ SalvadorBatista, Luis F. S.Torrecilha, Rafaela B. P. [UNESP]Silva, Rafaela B.Utsunomiya, Yuri T. [UNESP]Silva, Thais B. F.Tomokane, Thaise Y.Pacheco, Acacio D. [UNESP]Bosco, Anelise M. [UNESP]Paulan, Silvana C. [UNESP]Rossi, Claudio N.Costa, Gustavo N. O. [UNESP]Marcondes, Mary [UNESP]Ciarlini, Paulo C. [UNESP]Nunes, Caris M. [UNESP]Matta, Vania L. R.Laurenti, Marcia D.2021-06-25T12:30:02Z2021-06-25T12:30:02Z2020-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article12http://dx.doi.org/10.1016/j.vetpar.2020.109276Veterinary Parasitology. Amsterdam: Elsevier, v. 288, 12 p., 2020.0304-4017http://hdl.handle.net/11449/20981010.1016/j.vetpar.2020.109276WOS:000600787900002Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengVeterinary Parasitologyinfo:eu-repo/semantics/openAccess2024-09-04T19:16:18Zoai:repositorio.unesp.br:11449/209810Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-04T19:16:18Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Chromosomal segments may explain the antibody response cooperation for canine leishmaniasis pathogenesis |
title |
Chromosomal segments may explain the antibody response cooperation for canine leishmaniasis pathogenesis |
spellingShingle |
Chromosomal segments may explain the antibody response cooperation for canine leishmaniasis pathogenesis Batista, Luis F. S. GWAS Canine leishmaniasis Antiboby response Oxidative stress Renal-hepatic changes |
title_short |
Chromosomal segments may explain the antibody response cooperation for canine leishmaniasis pathogenesis |
title_full |
Chromosomal segments may explain the antibody response cooperation for canine leishmaniasis pathogenesis |
title_fullStr |
Chromosomal segments may explain the antibody response cooperation for canine leishmaniasis pathogenesis |
title_full_unstemmed |
Chromosomal segments may explain the antibody response cooperation for canine leishmaniasis pathogenesis |
title_sort |
Chromosomal segments may explain the antibody response cooperation for canine leishmaniasis pathogenesis |
author |
Batista, Luis F. S. |
author_facet |
Batista, Luis F. S. Torrecilha, Rafaela B. P. [UNESP] Silva, Rafaela B. Utsunomiya, Yuri T. [UNESP] Silva, Thais B. F. Tomokane, Thaise Y. Pacheco, Acacio D. [UNESP] Bosco, Anelise M. [UNESP] Paulan, Silvana C. [UNESP] Rossi, Claudio N. Costa, Gustavo N. O. [UNESP] Marcondes, Mary [UNESP] Ciarlini, Paulo C. [UNESP] Nunes, Caris M. [UNESP] Matta, Vania L. R. Laurenti, Marcia D. |
author_role |
author |
author2 |
Torrecilha, Rafaela B. P. [UNESP] Silva, Rafaela B. Utsunomiya, Yuri T. [UNESP] Silva, Thais B. F. Tomokane, Thaise Y. Pacheco, Acacio D. [UNESP] Bosco, Anelise M. [UNESP] Paulan, Silvana C. [UNESP] Rossi, Claudio N. Costa, Gustavo N. O. [UNESP] Marcondes, Mary [UNESP] Ciarlini, Paulo C. [UNESP] Nunes, Caris M. [UNESP] Matta, Vania L. R. Laurenti, Marcia D. |
author2_role |
author author author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Universidade Estadual Paulista (Unesp) Univ Salvador |
dc.contributor.author.fl_str_mv |
Batista, Luis F. S. Torrecilha, Rafaela B. P. [UNESP] Silva, Rafaela B. Utsunomiya, Yuri T. [UNESP] Silva, Thais B. F. Tomokane, Thaise Y. Pacheco, Acacio D. [UNESP] Bosco, Anelise M. [UNESP] Paulan, Silvana C. [UNESP] Rossi, Claudio N. Costa, Gustavo N. O. [UNESP] Marcondes, Mary [UNESP] Ciarlini, Paulo C. [UNESP] Nunes, Caris M. [UNESP] Matta, Vania L. R. Laurenti, Marcia D. |
dc.subject.por.fl_str_mv |
GWAS Canine leishmaniasis Antiboby response Oxidative stress Renal-hepatic changes |
topic |
GWAS Canine leishmaniasis Antiboby response Oxidative stress Renal-hepatic changes |
description |
Visceral leishmaniasis (VL) is marked by hyperactivation of a humoral response secreting high quantity of immunoglobulins (Igs) that are inaccessible to intracellular parasites. Here we investigated the contributions of the antibody response to the canine leishmaniasis pathogenesis. Using correlation and genome-wide association analysis, we investigated the relationship of anti-Leishmania infantum immunoglobulin classes levels with parasite burden, clinical response, renal/hepatic biochemical, and oxidative stress markers in dogs from endemic areas of VL. Immunoglobulin G (IgG) and IgA were positively correlated with parasite burden on lymph node and blood. Increased IgG, IgA and IgE levels were associated with severe canine leishmaniasis (CanL) whereas IgM was elevated in uninfected exposed dogs. Correlations of IgM, IgG and IgA with creatinine, urea, AST and ALT levels in the serum were suggested an involvement of those Igs with renal and hepatic changes. The correlogram of oxidative radicals and antioxidants revealed a likely relationship of IgM, IgG and IgA with oxidative stress and lipid pemxidation in the blood, suggested as mechanisms mediating tissue damage and CanL worsening. The gene mapping on chromosomal segments associated with the quantitative variation of immunoglobulin classes identified genetic signatures involved with reactive oxygen species generation, phagolysosome maturation and rupture, free iron availability, Thl/Th2 differenciation and, immunoglobulin clearance. The findings demonstrated the roles of the antibody response as resistance or susceptibility markers and mediators of CanL pathogenesis. In addition we pinpointed candidate genes as potential targets for the therapy against the damage caused by exacerbated antibody response and parasitism in VL. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-01 2021-06-25T12:30:02Z 2021-06-25T12:30:02Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.vetpar.2020.109276 Veterinary Parasitology. Amsterdam: Elsevier, v. 288, 12 p., 2020. 0304-4017 http://hdl.handle.net/11449/209810 10.1016/j.vetpar.2020.109276 WOS:000600787900002 |
url |
http://dx.doi.org/10.1016/j.vetpar.2020.109276 http://hdl.handle.net/11449/209810 |
identifier_str_mv |
Veterinary Parasitology. Amsterdam: Elsevier, v. 288, 12 p., 2020. 0304-4017 10.1016/j.vetpar.2020.109276 WOS:000600787900002 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Veterinary Parasitology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
12 |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
_version_ |
1810021421011697664 |