Influência da extensão da doença periodontal no perfil sanguíneo de ratos Wistar

Detalhes bibliográficos
Autor(a) principal: Guimarães, Gustav [UNESP]
Data de Publicação: 2016
Tipo de documento: Tese
Idioma: por
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://hdl.handle.net/11449/144733
Resumo: The aim of this study was to evaluate the influence of the periodontal disease extension on the blood profile of Wistar rats. Thirty rats were divided into 3 groups of 10 animals each: C control rats; DP1- rats with periodontal disease in 3 upper molars; DP2 - rats with periodontal disease in 3 upper and 3 lower molars. Periodontal disease was induced by ligature around the tooth according to each group. After 30 days, the animals were killed and 5ml of blood was collected for analyzes of blood count parameters, creatinine, triglycerides and cholesterol. Then the animals were sacrificed and the jaws removed and processed for histopathological analysis to characterize, in H.E. staining, the profile of periodontal disease in each animal. The results from the hematologic tissue were analyzed statistically by ANOVA and Tukey test, with 5% significance level. It can be observed in groups DP1 and DP2 presence of chronic inflammatory infiltrates, connective tissue attachment loss and alveolar bone cortical structure loss, presence of resorptions, presence of biofilm and bone sequestration. As for the blood profile, it is can observe a statistically significant difference between the control group and the groups DP1 and DP2 to the amount of leukocytes and lymphocytes (p <0.05). Further, DP2 group had a higher amount of leukocytes and neutrophils compared to DP1 and control group (p <0.05). It can be concluded that the presence of periodontal disease increases the amount of leukocytes, neutrophils, and lymphocytes in the blood of Wistar rats, and the extent of periodontal disease affects the quantification of leukocytes and neutrophils.
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spelling Influência da extensão da doença periodontal no perfil sanguíneo de ratos WistarInfluence of the periodontal disease extension on blood profile of Wistar ratsPeriodontal diseasesBlood cell countCreatinineBlood cellsDoença periodontalContagem de células sanguíneasCreatininaCélulas sanguíneasThe aim of this study was to evaluate the influence of the periodontal disease extension on the blood profile of Wistar rats. Thirty rats were divided into 3 groups of 10 animals each: C control rats; DP1- rats with periodontal disease in 3 upper molars; DP2 - rats with periodontal disease in 3 upper and 3 lower molars. Periodontal disease was induced by ligature around the tooth according to each group. After 30 days, the animals were killed and 5ml of blood was collected for analyzes of blood count parameters, creatinine, triglycerides and cholesterol. Then the animals were sacrificed and the jaws removed and processed for histopathological analysis to characterize, in H.E. staining, the profile of periodontal disease in each animal. The results from the hematologic tissue were analyzed statistically by ANOVA and Tukey test, with 5% significance level. It can be observed in groups DP1 and DP2 presence of chronic inflammatory infiltrates, connective tissue attachment loss and alveolar bone cortical structure loss, presence of resorptions, presence of biofilm and bone sequestration. As for the blood profile, it is can observe a statistically significant difference between the control group and the groups DP1 and DP2 to the amount of leukocytes and lymphocytes (p <0.05). Further, DP2 group had a higher amount of leukocytes and neutrophils compared to DP1 and control group (p <0.05). It can be concluded that the presence of periodontal disease increases the amount of leukocytes, neutrophils, and lymphocytes in the blood of Wistar rats, and the extent of periodontal disease affects the quantification of leukocytes and neutrophils.O objetivo deste trabalho foi investigar a influência da extensão da doença periodontal no perfil sanguíneo de ratos Wistar. Foram utilizados 30 ratos divididos em 3 grupos de 10 animais: C- ratos controle; DP1- ratos com doença periodontal em 3 molares superiores; DP2 – ratos com doença periodontal em 3 molares superiores e 3 inferiores. A doença periodontal foi induzida por meio da confecção de ligadura em torno do colo dentário dos molares selecionados de acordo com cada grupo. Após 30 dias, os animais foram anestesiados e, por meio de uma punção cardíaca, foi coletado 5ml de sangue para as análises dos parâmetros do hemograma, creatinina, triglicérides e colesterol. Em seguida, os animais foram sacrificados e as maxilas removidas e processadas para análise histopatológica em coloração de H.E. para caracterizar o perfil da doença periodontal em cada animal. Os resultados obtidos a partir do tecido hematológico foram analisados estaticamente por meio dos testes de ANOVA e Tukey, com nível de significância de 5%. Pode-se observar nos grupos DP1 e DP2 presença de infiltrado inflamatório crônico, perda de inserção conjuntiva, perda de estrutura óssea cortical e alveolar, presença de reabsorções dentárias, presença de biofilme e sequestro ósseo. Quanto ao perfil sanguíneo, pode-se observar diferença estatisticamente significante entre o grupo controle e os grupos DP1 e DP2 em relação à quantidade de leucócitos e linfócitos (p<0,05). Ainda, o grupo DP2 apresentou maior quantidade de leucócitos e neutrófilos em relação ao grupo DP1 e controle (p<0,05). Pode-se concluir que a presença da doença periodontal eleva a quantidade de leucócitos, neutrófilo e linfócitos no sangue de ratos Wistar, e que, a extensão da doença periodontal influencia na quantidade de leucócitos e neutrófilos.Universidade Estadual Paulista (Unesp)Cintra, Luciano Tavares Angelo [UNESP]Universidade Estadual Paulista (Unesp)Guimarães, Gustav [UNESP]2016-11-30T10:55:34Z2016-11-30T10:55:34Z2016-11-22info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisapplication/pdfhttp://hdl.handle.net/11449/14473300087639633004021073P59235743081667362porinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESP2024-01-29T06:31:16Zoai:repositorio.unesp.br:11449/144733Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-01-29T06:31:16Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Influência da extensão da doença periodontal no perfil sanguíneo de ratos Wistar
Influence of the periodontal disease extension on blood profile of Wistar rats
title Influência da extensão da doença periodontal no perfil sanguíneo de ratos Wistar
spellingShingle Influência da extensão da doença periodontal no perfil sanguíneo de ratos Wistar
Guimarães, Gustav [UNESP]
Periodontal diseases
Blood cell count
Creatinine
Blood cells
Doença periodontal
Contagem de células sanguíneas
Creatinina
Células sanguíneas
title_short Influência da extensão da doença periodontal no perfil sanguíneo de ratos Wistar
title_full Influência da extensão da doença periodontal no perfil sanguíneo de ratos Wistar
title_fullStr Influência da extensão da doença periodontal no perfil sanguíneo de ratos Wistar
title_full_unstemmed Influência da extensão da doença periodontal no perfil sanguíneo de ratos Wistar
title_sort Influência da extensão da doença periodontal no perfil sanguíneo de ratos Wistar
author Guimarães, Gustav [UNESP]
author_facet Guimarães, Gustav [UNESP]
author_role author
dc.contributor.none.fl_str_mv Cintra, Luciano Tavares Angelo [UNESP]
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Guimarães, Gustav [UNESP]
dc.subject.por.fl_str_mv Periodontal diseases
Blood cell count
Creatinine
Blood cells
Doença periodontal
Contagem de células sanguíneas
Creatinina
Células sanguíneas
topic Periodontal diseases
Blood cell count
Creatinine
Blood cells
Doença periodontal
Contagem de células sanguíneas
Creatinina
Células sanguíneas
description The aim of this study was to evaluate the influence of the periodontal disease extension on the blood profile of Wistar rats. Thirty rats were divided into 3 groups of 10 animals each: C control rats; DP1- rats with periodontal disease in 3 upper molars; DP2 - rats with periodontal disease in 3 upper and 3 lower molars. Periodontal disease was induced by ligature around the tooth according to each group. After 30 days, the animals were killed and 5ml of blood was collected for analyzes of blood count parameters, creatinine, triglycerides and cholesterol. Then the animals were sacrificed and the jaws removed and processed for histopathological analysis to characterize, in H.E. staining, the profile of periodontal disease in each animal. The results from the hematologic tissue were analyzed statistically by ANOVA and Tukey test, with 5% significance level. It can be observed in groups DP1 and DP2 presence of chronic inflammatory infiltrates, connective tissue attachment loss and alveolar bone cortical structure loss, presence of resorptions, presence of biofilm and bone sequestration. As for the blood profile, it is can observe a statistically significant difference between the control group and the groups DP1 and DP2 to the amount of leukocytes and lymphocytes (p <0.05). Further, DP2 group had a higher amount of leukocytes and neutrophils compared to DP1 and control group (p <0.05). It can be concluded that the presence of periodontal disease increases the amount of leukocytes, neutrophils, and lymphocytes in the blood of Wistar rats, and the extent of periodontal disease affects the quantification of leukocytes and neutrophils.
publishDate 2016
dc.date.none.fl_str_mv 2016-11-30T10:55:34Z
2016-11-30T10:55:34Z
2016-11-22
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/doctoralThesis
format doctoralThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/11449/144733
000876396
33004021073P5
9235743081667362
url http://hdl.handle.net/11449/144733
identifier_str_mv 000876396
33004021073P5
9235743081667362
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Estadual Paulista (Unesp)
publisher.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1803650386260656128

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