Validação de radioimunoensaio para quantificação de leptina plasmática bovina
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://www.revistas.usp.br/bjvras/article/view/26843 http://hdl.handle.net/11449/72186 |
Resumo: | Due necessity of better understanding leptin and reproduction relations, a specific radioimmunoassay (RIA) to bovine leptin was avalidated. First, an antibody production protocol was developed using recombinant equine leptin inoculated in a rabbit, that results in 28,05% of maximum binding (MB) 105 days after the protocol beginning. The tests of validations verified parallelism between standard curve and dilutions of high and low controls (P < 0,01). Antibody against equine leptin showed specificity to bovine leptin (P < 0,01). The recuperation tax of bovine leptin by antibody against recombinant equine leptin was from 98,4 to 101,6% (P < 0, 01). When the samples were stored in ambient temperature or refrigerated to 4°C, ligation stability was verified (P > 0,2), however, temperatures above 37°C impaired the bovine leptin recuperation. The use of assay buffer with or without bovine plasma did not show any difference (P > 0,3). These results showed that the antibody produced in rabbit against equine leptin were able to detect plasmatic bovine leptin, and that the RIA to bovine leptin quantification had adequate characteristics to the development of a valid assay. |
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Validação de radioimunoensaio para quantificação de leptina plasmática bovinaImunoradioassay validation to plasmatic bovine leptin quantificationAntibodyLeptinRadioimmunoassayValidationBovinaeEquidaeOryctolagus cuniculusDue necessity of better understanding leptin and reproduction relations, a specific radioimmunoassay (RIA) to bovine leptin was avalidated. First, an antibody production protocol was developed using recombinant equine leptin inoculated in a rabbit, that results in 28,05% of maximum binding (MB) 105 days after the protocol beginning. The tests of validations verified parallelism between standard curve and dilutions of high and low controls (P < 0,01). Antibody against equine leptin showed specificity to bovine leptin (P < 0,01). The recuperation tax of bovine leptin by antibody against recombinant equine leptin was from 98,4 to 101,6% (P < 0, 01). When the samples were stored in ambient temperature or refrigerated to 4°C, ligation stability was verified (P > 0,2), however, temperatures above 37°C impaired the bovine leptin recuperation. The use of assay buffer with or without bovine plasma did not show any difference (P > 0,3). These results showed that the antibody produced in rabbit against equine leptin were able to detect plasmatic bovine leptin, and that the RIA to bovine leptin quantification had adequate characteristics to the development of a valid assay.Médica VeterináriaLaboratório de Fisiologia e Endocrinologia Animal Universidade Estadual Paulista, Araçatuba-SPLaboratório de Fisiologia e Endocrinologia Animal Universidade Estadual Paulista, Araçatuba-SPMédica VeterináriaUniversidade Estadual Paulista (Unesp)Beltran, Maria Paula [UNESP]Nogueira, Guilherme de Paula2014-05-27T11:25:24Z2014-05-27T11:25:24Z2010-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article5-12application/pdfhttp://www.revistas.usp.br/bjvras/article/view/26843Brazilian Journal of Veterinary Research and Animal Science, v. 47, n. 1, p. 5-12, 2010.1413-95961678-4456http://hdl.handle.net/11449/721862-s2.0-786494277622-s2.0-78649427762.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPporBrazilian Journal of Veterinary Research and Animal Science0,2250,225info:eu-repo/semantics/openAccess2023-10-03T06:02:00Zoai:repositorio.unesp.br:11449/72186Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T13:50:43.458442Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Validação de radioimunoensaio para quantificação de leptina plasmática bovina Imunoradioassay validation to plasmatic bovine leptin quantification |
title |
Validação de radioimunoensaio para quantificação de leptina plasmática bovina |
spellingShingle |
Validação de radioimunoensaio para quantificação de leptina plasmática bovina Beltran, Maria Paula [UNESP] Antibody Leptin Radioimmunoassay Validation Bovinae Equidae Oryctolagus cuniculus |
title_short |
Validação de radioimunoensaio para quantificação de leptina plasmática bovina |
title_full |
Validação de radioimunoensaio para quantificação de leptina plasmática bovina |
title_fullStr |
Validação de radioimunoensaio para quantificação de leptina plasmática bovina |
title_full_unstemmed |
Validação de radioimunoensaio para quantificação de leptina plasmática bovina |
title_sort |
Validação de radioimunoensaio para quantificação de leptina plasmática bovina |
author |
Beltran, Maria Paula [UNESP] |
author_facet |
Beltran, Maria Paula [UNESP] Nogueira, Guilherme de Paula |
author_role |
author |
author2 |
Nogueira, Guilherme de Paula |
author2_role |
author |
dc.contributor.none.fl_str_mv |
Médica Veterinária Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Beltran, Maria Paula [UNESP] Nogueira, Guilherme de Paula |
dc.subject.por.fl_str_mv |
Antibody Leptin Radioimmunoassay Validation Bovinae Equidae Oryctolagus cuniculus |
topic |
Antibody Leptin Radioimmunoassay Validation Bovinae Equidae Oryctolagus cuniculus |
description |
Due necessity of better understanding leptin and reproduction relations, a specific radioimmunoassay (RIA) to bovine leptin was avalidated. First, an antibody production protocol was developed using recombinant equine leptin inoculated in a rabbit, that results in 28,05% of maximum binding (MB) 105 days after the protocol beginning. The tests of validations verified parallelism between standard curve and dilutions of high and low controls (P < 0,01). Antibody against equine leptin showed specificity to bovine leptin (P < 0,01). The recuperation tax of bovine leptin by antibody against recombinant equine leptin was from 98,4 to 101,6% (P < 0, 01). When the samples were stored in ambient temperature or refrigerated to 4°C, ligation stability was verified (P > 0,2), however, temperatures above 37°C impaired the bovine leptin recuperation. The use of assay buffer with or without bovine plasma did not show any difference (P > 0,3). These results showed that the antibody produced in rabbit against equine leptin were able to detect plasmatic bovine leptin, and that the RIA to bovine leptin quantification had adequate characteristics to the development of a valid assay. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-12-01 2014-05-27T11:25:24Z 2014-05-27T11:25:24Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.revistas.usp.br/bjvras/article/view/26843 Brazilian Journal of Veterinary Research and Animal Science, v. 47, n. 1, p. 5-12, 2010. 1413-9596 1678-4456 http://hdl.handle.net/11449/72186 2-s2.0-78649427762 2-s2.0-78649427762.pdf |
url |
http://www.revistas.usp.br/bjvras/article/view/26843 http://hdl.handle.net/11449/72186 |
identifier_str_mv |
Brazilian Journal of Veterinary Research and Animal Science, v. 47, n. 1, p. 5-12, 2010. 1413-9596 1678-4456 2-s2.0-78649427762 2-s2.0-78649427762.pdf |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
Brazilian Journal of Veterinary Research and Animal Science 0,225 0,225 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
5-12 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128282798850048 |