Antimicrobial photodynamic therapy mediated by methylene blue-loaded polymeric micelles against Streptococcus mutans and Candida albicans biofilms
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.pdpdt.2023.103285 http://hdl.handle.net/11449/249567 |
Resumo: | Background: Streptococcus mutans and Candida albicans can colonize the teeth, the oral cavity as biofilm and can cause oral infections. Thus, strategies to prevent and control oral biofilms are requested. The present study aims the development and characterization of methylene blue (MB)-loaded polymeric micelles for antimicrobial photodynamic therapy (aPDT) against Streptococcus mutans and Candida albicans biofilms Methods: MB-loaded polymeric micelles were produced and characterized by particle size, polydispersity index, morphology, zeta potential, stability, MB release profile, and antimicrobial effect against S. mutans and C. albicans biofilms. Results: MB-loaded polymeric micelles showed a reduced particle size, moderate polydisperse profile, spherical and neutral shape, which demonstrated to be promising features to allow micelles penetration into biofilms. Antimicrobial effect against bacterial and yeast biofilms was demonstrated once MB was irradiated by light under 660 nm (aPDT). Furthermore, MB-loaded polymeric micelles showed significant inhibition of S. mutans and C. albicans biofilms. Furthermore, the treatment with MB-micelles incubated with high pre-incubation times (15 and 30 min) were more effective than 5 min. It can be explained by the time required for this nanosystem to penetrate the innermost layer of biofilms and release MB for aPDT. Conclusion: MB-loaded polymeric micelles can effectively decrease the bacteria and yeast viability and it may cause positive impacts in the clinical practice. Thus, the developed formulation showed potential in the treatment to remove oral biofilms, but clinical studies are needed to confirm its potential |
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Antimicrobial photodynamic therapy mediated by methylene blue-loaded polymeric micelles against Streptococcus mutans and Candida albicans biofilmsMethylene blueNanoparticlesNanosystemsPhotodynamic therapyphotosensitizerPluronic® F127Poloxamer 407Background: Streptococcus mutans and Candida albicans can colonize the teeth, the oral cavity as biofilm and can cause oral infections. Thus, strategies to prevent and control oral biofilms are requested. The present study aims the development and characterization of methylene blue (MB)-loaded polymeric micelles for antimicrobial photodynamic therapy (aPDT) against Streptococcus mutans and Candida albicans biofilms Methods: MB-loaded polymeric micelles were produced and characterized by particle size, polydispersity index, morphology, zeta potential, stability, MB release profile, and antimicrobial effect against S. mutans and C. albicans biofilms. Results: MB-loaded polymeric micelles showed a reduced particle size, moderate polydisperse profile, spherical and neutral shape, which demonstrated to be promising features to allow micelles penetration into biofilms. Antimicrobial effect against bacterial and yeast biofilms was demonstrated once MB was irradiated by light under 660 nm (aPDT). Furthermore, MB-loaded polymeric micelles showed significant inhibition of S. mutans and C. albicans biofilms. Furthermore, the treatment with MB-micelles incubated with high pre-incubation times (15 and 30 min) were more effective than 5 min. It can be explained by the time required for this nanosystem to penetrate the innermost layer of biofilms and release MB for aPDT. Conclusion: MB-loaded polymeric micelles can effectively decrease the bacteria and yeast viability and it may cause positive impacts in the clinical practice. Thus, the developed formulation showed potential in the treatment to remove oral biofilms, but clinical studies are needed to confirm its potentialSchool of Pharmaceutical Science Sao Paulo State University (UNESP), Sao PauloDepartament of Restorative Dentistry School of Dentistry Sao Paulo State University (UNESP), Sao PauloSchool of Pharmaceutical Science Sao Paulo State University (UNESP), Sao PauloDepartament of Restorative Dentistry School of Dentistry Sao Paulo State University (UNESP), Sao PauloUniversidade Estadual Paulista (UNESP)Soares, Jonas Corsino Maduro [UNESP]Luiz, Marcela Tavares [UNESP]Oshiro Junior, João Augusto [UNESP]Besegato, João Felipe [UNESP]de Melo, Priscila Borges Gobbo [UNESP]Rastelli, Alessandra Nara de Souza [UNESP]Chorilli, Marlus [UNESP]2023-07-29T16:03:15Z2023-07-29T16:03:15Z2023-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.pdpdt.2023.103285Photodiagnosis and Photodynamic Therapy, v. 41.1873-15971572-1000http://hdl.handle.net/11449/24956710.1016/j.pdpdt.2023.1032852-s2.0-85146477411Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPhotodiagnosis and Photodynamic Therapyinfo:eu-repo/semantics/openAccess2023-07-29T16:03:15Zoai:repositorio.unesp.br:11449/249567Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-07-29T16:03:15Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Antimicrobial photodynamic therapy mediated by methylene blue-loaded polymeric micelles against Streptococcus mutans and Candida albicans biofilms |
title |
Antimicrobial photodynamic therapy mediated by methylene blue-loaded polymeric micelles against Streptococcus mutans and Candida albicans biofilms |
spellingShingle |
Antimicrobial photodynamic therapy mediated by methylene blue-loaded polymeric micelles against Streptococcus mutans and Candida albicans biofilms Soares, Jonas Corsino Maduro [UNESP] Methylene blue Nanoparticles Nanosystems Photodynamic therapy photosensitizer Pluronic® F127 Poloxamer 407 |
title_short |
Antimicrobial photodynamic therapy mediated by methylene blue-loaded polymeric micelles against Streptococcus mutans and Candida albicans biofilms |
title_full |
Antimicrobial photodynamic therapy mediated by methylene blue-loaded polymeric micelles against Streptococcus mutans and Candida albicans biofilms |
title_fullStr |
Antimicrobial photodynamic therapy mediated by methylene blue-loaded polymeric micelles against Streptococcus mutans and Candida albicans biofilms |
title_full_unstemmed |
Antimicrobial photodynamic therapy mediated by methylene blue-loaded polymeric micelles against Streptococcus mutans and Candida albicans biofilms |
title_sort |
Antimicrobial photodynamic therapy mediated by methylene blue-loaded polymeric micelles against Streptococcus mutans and Candida albicans biofilms |
author |
Soares, Jonas Corsino Maduro [UNESP] |
author_facet |
Soares, Jonas Corsino Maduro [UNESP] Luiz, Marcela Tavares [UNESP] Oshiro Junior, João Augusto [UNESP] Besegato, João Felipe [UNESP] de Melo, Priscila Borges Gobbo [UNESP] Rastelli, Alessandra Nara de Souza [UNESP] Chorilli, Marlus [UNESP] |
author_role |
author |
author2 |
Luiz, Marcela Tavares [UNESP] Oshiro Junior, João Augusto [UNESP] Besegato, João Felipe [UNESP] de Melo, Priscila Borges Gobbo [UNESP] Rastelli, Alessandra Nara de Souza [UNESP] Chorilli, Marlus [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Soares, Jonas Corsino Maduro [UNESP] Luiz, Marcela Tavares [UNESP] Oshiro Junior, João Augusto [UNESP] Besegato, João Felipe [UNESP] de Melo, Priscila Borges Gobbo [UNESP] Rastelli, Alessandra Nara de Souza [UNESP] Chorilli, Marlus [UNESP] |
dc.subject.por.fl_str_mv |
Methylene blue Nanoparticles Nanosystems Photodynamic therapy photosensitizer Pluronic® F127 Poloxamer 407 |
topic |
Methylene blue Nanoparticles Nanosystems Photodynamic therapy photosensitizer Pluronic® F127 Poloxamer 407 |
description |
Background: Streptococcus mutans and Candida albicans can colonize the teeth, the oral cavity as biofilm and can cause oral infections. Thus, strategies to prevent and control oral biofilms are requested. The present study aims the development and characterization of methylene blue (MB)-loaded polymeric micelles for antimicrobial photodynamic therapy (aPDT) against Streptococcus mutans and Candida albicans biofilms Methods: MB-loaded polymeric micelles were produced and characterized by particle size, polydispersity index, morphology, zeta potential, stability, MB release profile, and antimicrobial effect against S. mutans and C. albicans biofilms. Results: MB-loaded polymeric micelles showed a reduced particle size, moderate polydisperse profile, spherical and neutral shape, which demonstrated to be promising features to allow micelles penetration into biofilms. Antimicrobial effect against bacterial and yeast biofilms was demonstrated once MB was irradiated by light under 660 nm (aPDT). Furthermore, MB-loaded polymeric micelles showed significant inhibition of S. mutans and C. albicans biofilms. Furthermore, the treatment with MB-micelles incubated with high pre-incubation times (15 and 30 min) were more effective than 5 min. It can be explained by the time required for this nanosystem to penetrate the innermost layer of biofilms and release MB for aPDT. Conclusion: MB-loaded polymeric micelles can effectively decrease the bacteria and yeast viability and it may cause positive impacts in the clinical practice. Thus, the developed formulation showed potential in the treatment to remove oral biofilms, but clinical studies are needed to confirm its potential |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-07-29T16:03:15Z 2023-07-29T16:03:15Z 2023-03-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.pdpdt.2023.103285 Photodiagnosis and Photodynamic Therapy, v. 41. 1873-1597 1572-1000 http://hdl.handle.net/11449/249567 10.1016/j.pdpdt.2023.103285 2-s2.0-85146477411 |
url |
http://dx.doi.org/10.1016/j.pdpdt.2023.103285 http://hdl.handle.net/11449/249567 |
identifier_str_mv |
Photodiagnosis and Photodynamic Therapy, v. 41. 1873-1597 1572-1000 10.1016/j.pdpdt.2023.103285 2-s2.0-85146477411 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Photodiagnosis and Photodynamic Therapy |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799964973510688768 |