Budesonide-hydroxypropyl-beta-cyclodextrin inclusion complex in binary poloxamer 407/403 system for ulcerative colitis treatment: A physico-chemical study from micelles to hydrogels
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.colsurfb.2015.11.048 http://hdl.handle.net/11449/165036 |
Resumo: | Budesonide (BUD) is a glucocorticoid widely used for the treatment of ulcerative colitis. In this work, we propose the study of the system BUD-HP-beta-CD inclusion complex incorporated into PL 407 and PL407-PL403 thermoreversible hydrogels, considering physico-chemical and pharmaceutical aspects. Complexation between BUD and HP-beta-CD was confirmed by phase solubility studies (1:1 stoichiometry, Kc = 8662.8 M-1), DSC, FTIR and microscopy analyzes. BUD solubility in simulated upper and lower colon fluids was improved in a dependence of HP-beta-CD and PL 407 or PL407-PL403 association. Micellar hydrodynamic diameter studies showed the interaction between HP-beta-CD and PL blocks, as well as the reorganization of the micellar system in the presence of BUD and its inclusion complex. Micellization temperature (T-m) was not shifted, but sol-gel phase transition studies showed that in the presence of BUD, HP-beta-CD or BUD:HP-beta-CD complex, the association PL407-PL403 favored the gel formation close to the physiological temperature. Physico-chemical and in vitro release assays studies revealed no competitive displacement of BUD from the HP-beta-CD cavity evoked by PL407 or PL407-PL403 addition. These findings point out the BUD-HP-beta-CD in PL-based hydrogels as strategies for future investigations on development of new pharmaceutical formulations for the treatment of ulcerative colitis. (C) 2015 Elsevier B.V. All rights reserved. |
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Budesonide-hydroxypropyl-beta-cyclodextrin inclusion complex in binary poloxamer 407/403 system for ulcerative colitis treatment: A physico-chemical study from micelles to hydrogelsBudesonideCyclodextrinPoloxamerMicelleHydrogelUlcerative colitisPolyrotaxaneBudesonide (BUD) is a glucocorticoid widely used for the treatment of ulcerative colitis. In this work, we propose the study of the system BUD-HP-beta-CD inclusion complex incorporated into PL 407 and PL407-PL403 thermoreversible hydrogels, considering physico-chemical and pharmaceutical aspects. Complexation between BUD and HP-beta-CD was confirmed by phase solubility studies (1:1 stoichiometry, Kc = 8662.8 M-1), DSC, FTIR and microscopy analyzes. BUD solubility in simulated upper and lower colon fluids was improved in a dependence of HP-beta-CD and PL 407 or PL407-PL403 association. Micellar hydrodynamic diameter studies showed the interaction between HP-beta-CD and PL blocks, as well as the reorganization of the micellar system in the presence of BUD and its inclusion complex. Micellization temperature (T-m) was not shifted, but sol-gel phase transition studies showed that in the presence of BUD, HP-beta-CD or BUD:HP-beta-CD complex, the association PL407-PL403 favored the gel formation close to the physiological temperature. Physico-chemical and in vitro release assays studies revealed no competitive displacement of BUD from the HP-beta-CD cavity evoked by PL407 or PL407-PL403 addition. These findings point out the BUD-HP-beta-CD in PL-based hydrogels as strategies for future investigations on development of new pharmaceutical formulations for the treatment of ulcerative colitis. (C) 2015 Elsevier B.V. All rights reserved.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundacao de Amparo Pesquisa do Estado de Sao PauloConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Univ Fed Abc, Ctr Ciencias Nat & Humanas, BR-09210580 Santo Andre, SP, BrazilState Univ Julio de Mesquita Filho, Dept Enviroment Engn, Sorocaba, SP, BrazilUniv Estadual Campinas, Inst Biol, Dept Biochem, Campinas, SP, BrazilFac Dent Sao Leopoldo Mand, Campinas, SP, BrazilState Univ Julio de Mesquita Filho, Dept Enviroment Engn, Sorocaba, SP, BrazilFundacao de Amparo Pesquisa do Estado de Sao Paulo: FAPESP 2014/26200-9Fundacao de Amparo Pesquisa do Estado de Sao Paulo: 2014/14457-5CNPq: CNPq 487619/2012-9CNPq: 309612/2013-6Elsevier B.V.Universidade Federal do ABC (UFABC)Universidade Estadual Paulista (Unesp)Universidade Estadual de Campinas (UNICAMP)Fac Dent Sao Leopoldo MandSantos Akkari, Alessandra CristinaRamos Campos, Estefania Vangelie [UNESP]Keppler, Artur FranzFraceto, Leonardo Fernandes [UNESP]Paula, Eneida deTofoli, Giovana RadomilleAraujo, Daniele Ribeiro de2018-11-27T07:08:26Z2018-11-27T07:08:26Z2016-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article138-147application/pdfhttp://dx.doi.org/10.1016/j.colsurfb.2015.11.048Colloids And Surfaces B-biointerfaces. Amsterdam: Elsevier Science Bv, v. 138, p. 138-147, 2016.0927-7765http://hdl.handle.net/11449/16503610.1016/j.colsurfb.2015.11.048WOS:000368205100017WOS000368205100017.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengColloids And Surfaces B-biointerfaces1,071info:eu-repo/semantics/openAccess2023-11-13T06:16:00Zoai:repositorio.unesp.br:11449/165036Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:36:02.528306Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Budesonide-hydroxypropyl-beta-cyclodextrin inclusion complex in binary poloxamer 407/403 system for ulcerative colitis treatment: A physico-chemical study from micelles to hydrogels |
title |
Budesonide-hydroxypropyl-beta-cyclodextrin inclusion complex in binary poloxamer 407/403 system for ulcerative colitis treatment: A physico-chemical study from micelles to hydrogels |
spellingShingle |
Budesonide-hydroxypropyl-beta-cyclodextrin inclusion complex in binary poloxamer 407/403 system for ulcerative colitis treatment: A physico-chemical study from micelles to hydrogels Santos Akkari, Alessandra Cristina Budesonide Cyclodextrin Poloxamer Micelle Hydrogel Ulcerative colitis Polyrotaxane |
title_short |
Budesonide-hydroxypropyl-beta-cyclodextrin inclusion complex in binary poloxamer 407/403 system for ulcerative colitis treatment: A physico-chemical study from micelles to hydrogels |
title_full |
Budesonide-hydroxypropyl-beta-cyclodextrin inclusion complex in binary poloxamer 407/403 system for ulcerative colitis treatment: A physico-chemical study from micelles to hydrogels |
title_fullStr |
Budesonide-hydroxypropyl-beta-cyclodextrin inclusion complex in binary poloxamer 407/403 system for ulcerative colitis treatment: A physico-chemical study from micelles to hydrogels |
title_full_unstemmed |
Budesonide-hydroxypropyl-beta-cyclodextrin inclusion complex in binary poloxamer 407/403 system for ulcerative colitis treatment: A physico-chemical study from micelles to hydrogels |
title_sort |
Budesonide-hydroxypropyl-beta-cyclodextrin inclusion complex in binary poloxamer 407/403 system for ulcerative colitis treatment: A physico-chemical study from micelles to hydrogels |
author |
Santos Akkari, Alessandra Cristina |
author_facet |
Santos Akkari, Alessandra Cristina Ramos Campos, Estefania Vangelie [UNESP] Keppler, Artur Franz Fraceto, Leonardo Fernandes [UNESP] Paula, Eneida de Tofoli, Giovana Radomille Araujo, Daniele Ribeiro de |
author_role |
author |
author2 |
Ramos Campos, Estefania Vangelie [UNESP] Keppler, Artur Franz Fraceto, Leonardo Fernandes [UNESP] Paula, Eneida de Tofoli, Giovana Radomille Araujo, Daniele Ribeiro de |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal do ABC (UFABC) Universidade Estadual Paulista (Unesp) Universidade Estadual de Campinas (UNICAMP) Fac Dent Sao Leopoldo Mand |
dc.contributor.author.fl_str_mv |
Santos Akkari, Alessandra Cristina Ramos Campos, Estefania Vangelie [UNESP] Keppler, Artur Franz Fraceto, Leonardo Fernandes [UNESP] Paula, Eneida de Tofoli, Giovana Radomille Araujo, Daniele Ribeiro de |
dc.subject.por.fl_str_mv |
Budesonide Cyclodextrin Poloxamer Micelle Hydrogel Ulcerative colitis Polyrotaxane |
topic |
Budesonide Cyclodextrin Poloxamer Micelle Hydrogel Ulcerative colitis Polyrotaxane |
description |
Budesonide (BUD) is a glucocorticoid widely used for the treatment of ulcerative colitis. In this work, we propose the study of the system BUD-HP-beta-CD inclusion complex incorporated into PL 407 and PL407-PL403 thermoreversible hydrogels, considering physico-chemical and pharmaceutical aspects. Complexation between BUD and HP-beta-CD was confirmed by phase solubility studies (1:1 stoichiometry, Kc = 8662.8 M-1), DSC, FTIR and microscopy analyzes. BUD solubility in simulated upper and lower colon fluids was improved in a dependence of HP-beta-CD and PL 407 or PL407-PL403 association. Micellar hydrodynamic diameter studies showed the interaction between HP-beta-CD and PL blocks, as well as the reorganization of the micellar system in the presence of BUD and its inclusion complex. Micellization temperature (T-m) was not shifted, but sol-gel phase transition studies showed that in the presence of BUD, HP-beta-CD or BUD:HP-beta-CD complex, the association PL407-PL403 favored the gel formation close to the physiological temperature. Physico-chemical and in vitro release assays studies revealed no competitive displacement of BUD from the HP-beta-CD cavity evoked by PL407 or PL407-PL403 addition. These findings point out the BUD-HP-beta-CD in PL-based hydrogels as strategies for future investigations on development of new pharmaceutical formulations for the treatment of ulcerative colitis. (C) 2015 Elsevier B.V. All rights reserved. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-02-01 2018-11-27T07:08:26Z 2018-11-27T07:08:26Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.colsurfb.2015.11.048 Colloids And Surfaces B-biointerfaces. Amsterdam: Elsevier Science Bv, v. 138, p. 138-147, 2016. 0927-7765 http://hdl.handle.net/11449/165036 10.1016/j.colsurfb.2015.11.048 WOS:000368205100017 WOS000368205100017.pdf |
url |
http://dx.doi.org/10.1016/j.colsurfb.2015.11.048 http://hdl.handle.net/11449/165036 |
identifier_str_mv |
Colloids And Surfaces B-biointerfaces. Amsterdam: Elsevier Science Bv, v. 138, p. 138-147, 2016. 0927-7765 10.1016/j.colsurfb.2015.11.048 WOS:000368205100017 WOS000368205100017.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Colloids And Surfaces B-biointerfaces 1,071 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
138-147 application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128834032107520 |