Nature of the interactions between hypocrealean fungi and the mutualistic fungus of leaf-cutter ants
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s10482-016-0826-y http://hdl.handle.net/11449/169300 |
Resumo: | Leaf-cutter ants cultivate and feed on the mutualistic fungus, Leucoagaricus gongylophorus, which is threatened by parasitic fungi of the genus Escovopsis. The mechanism of Escovopsis parasitism is poorly understood. Here, we assessed the nature of the antagonism of different Escovopsis species against its host. We also evaluated the potential antagonism of Escovopsioides, a recently described fungal genus from the attine ant environment whose role in the colonies of these insects is unknown. We performed dual-culture assays to assess the interactions between L. gongylophorus and both fungi. We also evaluated the antifungal activity of compounds secreted by the latter on L. gongylophorus growth using crude extracts of Escovopsis spp. and Escovopsioides nivea obtained either in (1) absence or (2) presence of the mutualistic fungus. The physical interaction between these fungi and the mutualistic fungus was examined under scanning electron microscopy (SEM). Escovopsis spp. and E. nivea negatively affected the growth of L. gongylophorus, which was also significantly inhibited by both types of crude extract. These results indicate that Escovopsis spp. and E. nivea produce antifungal metabolites against the mutualistic fungus. SEM showed that Escovopsis spp. and E. nivea maintained physical contact with the mutualistic fungus, though no specialised structures related to mycoparasitism were observed. These results showed that Escovopsis is a destructive mycoparasite that needs physical contact for the death of the mutualistic fungus to occur. Also, our findings suggest that E. nivea is an antagonist of the ant fungal cultivar. |
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Nature of the interactions between hypocrealean fungi and the mutualistic fungus of leaf-cutter antsAntagonismHypocrealesMycoparasitismTribe AttiniLeaf-cutter ants cultivate and feed on the mutualistic fungus, Leucoagaricus gongylophorus, which is threatened by parasitic fungi of the genus Escovopsis. The mechanism of Escovopsis parasitism is poorly understood. Here, we assessed the nature of the antagonism of different Escovopsis species against its host. We also evaluated the potential antagonism of Escovopsioides, a recently described fungal genus from the attine ant environment whose role in the colonies of these insects is unknown. We performed dual-culture assays to assess the interactions between L. gongylophorus and both fungi. We also evaluated the antifungal activity of compounds secreted by the latter on L. gongylophorus growth using crude extracts of Escovopsis spp. and Escovopsioides nivea obtained either in (1) absence or (2) presence of the mutualistic fungus. The physical interaction between these fungi and the mutualistic fungus was examined under scanning electron microscopy (SEM). Escovopsis spp. and E. nivea negatively affected the growth of L. gongylophorus, which was also significantly inhibited by both types of crude extract. These results indicate that Escovopsis spp. and E. nivea produce antifungal metabolites against the mutualistic fungus. SEM showed that Escovopsis spp. and E. nivea maintained physical contact with the mutualistic fungus, though no specialised structures related to mycoparasitism were observed. These results showed that Escovopsis is a destructive mycoparasite that needs physical contact for the death of the mutualistic fungus to occur. Also, our findings suggest that E. nivea is an antagonist of the ant fungal cultivar.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Departamento de Bioquímica e Microbiologia Universidade Estadual Paulista (UNESP), Av. 24A, 1515, Bela VistaDepartamento de Química Universidade Federal de São Carlos, Rodovia Washington Luís, km 235Centro Interdisciplinar de Ciências da Vida Universidade Federal da Integração Latino-Americana, Av. Tancredo Neves, 6731 - Bloco 6, Caixa Postal 2044Instituto de Ciências Agrárias Universidade Federal de Uberlândia, Campus Monte Carmelo. Rodovia LMG-746, km 1Departamento de Bioquímica e Microbiologia Universidade Estadual Paulista (UNESP), Av. 24A, 1515, Bela VistaCAPES: 1455/2008FAPESP: 2011/16765-0Universidade Estadual Paulista (Unesp)Universidade Federal de São Carlos (UFSCar)Universidade Federal da Integração Latino-AmericanaUniversidade Federal de Uberlândia (UFU)Varanda-Haifig, Sadala Schmidt [UNESP]Albarici, Tatiane ReginaNunes, Pablo HenriqueHaifig, IvesVieira, Paulo CezarRodrigues, Andre [UNESP]2018-12-11T16:45:16Z2018-12-11T16:45:16Z2017-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article593-605application/pdfhttp://dx.doi.org/10.1007/s10482-016-0826-yAntonie van Leeuwenhoek, International Journal of General and Molecular Microbiology, v. 110, n. 4, p. 593-605, 2017.1572-96990003-6072http://hdl.handle.net/11449/16930010.1007/s10482-016-0826-y2-s2.0-850074160772-s2.0-85007416077.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAntonie van Leeuwenhoek, International Journal of General and Molecular Microbiology0,8340,834info:eu-repo/semantics/openAccess2023-10-12T06:09:55Zoai:repositorio.unesp.br:11449/169300Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T14:42:38.897147Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Nature of the interactions between hypocrealean fungi and the mutualistic fungus of leaf-cutter ants |
title |
Nature of the interactions between hypocrealean fungi and the mutualistic fungus of leaf-cutter ants |
spellingShingle |
Nature of the interactions between hypocrealean fungi and the mutualistic fungus of leaf-cutter ants Varanda-Haifig, Sadala Schmidt [UNESP] Antagonism Hypocreales Mycoparasitism Tribe Attini |
title_short |
Nature of the interactions between hypocrealean fungi and the mutualistic fungus of leaf-cutter ants |
title_full |
Nature of the interactions between hypocrealean fungi and the mutualistic fungus of leaf-cutter ants |
title_fullStr |
Nature of the interactions between hypocrealean fungi and the mutualistic fungus of leaf-cutter ants |
title_full_unstemmed |
Nature of the interactions between hypocrealean fungi and the mutualistic fungus of leaf-cutter ants |
title_sort |
Nature of the interactions between hypocrealean fungi and the mutualistic fungus of leaf-cutter ants |
author |
Varanda-Haifig, Sadala Schmidt [UNESP] |
author_facet |
Varanda-Haifig, Sadala Schmidt [UNESP] Albarici, Tatiane Regina Nunes, Pablo Henrique Haifig, Ives Vieira, Paulo Cezar Rodrigues, Andre [UNESP] |
author_role |
author |
author2 |
Albarici, Tatiane Regina Nunes, Pablo Henrique Haifig, Ives Vieira, Paulo Cezar Rodrigues, Andre [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade Federal de São Carlos (UFSCar) Universidade Federal da Integração Latino-Americana Universidade Federal de Uberlândia (UFU) |
dc.contributor.author.fl_str_mv |
Varanda-Haifig, Sadala Schmidt [UNESP] Albarici, Tatiane Regina Nunes, Pablo Henrique Haifig, Ives Vieira, Paulo Cezar Rodrigues, Andre [UNESP] |
dc.subject.por.fl_str_mv |
Antagonism Hypocreales Mycoparasitism Tribe Attini |
topic |
Antagonism Hypocreales Mycoparasitism Tribe Attini |
description |
Leaf-cutter ants cultivate and feed on the mutualistic fungus, Leucoagaricus gongylophorus, which is threatened by parasitic fungi of the genus Escovopsis. The mechanism of Escovopsis parasitism is poorly understood. Here, we assessed the nature of the antagonism of different Escovopsis species against its host. We also evaluated the potential antagonism of Escovopsioides, a recently described fungal genus from the attine ant environment whose role in the colonies of these insects is unknown. We performed dual-culture assays to assess the interactions between L. gongylophorus and both fungi. We also evaluated the antifungal activity of compounds secreted by the latter on L. gongylophorus growth using crude extracts of Escovopsis spp. and Escovopsioides nivea obtained either in (1) absence or (2) presence of the mutualistic fungus. The physical interaction between these fungi and the mutualistic fungus was examined under scanning electron microscopy (SEM). Escovopsis spp. and E. nivea negatively affected the growth of L. gongylophorus, which was also significantly inhibited by both types of crude extract. These results indicate that Escovopsis spp. and E. nivea produce antifungal metabolites against the mutualistic fungus. SEM showed that Escovopsis spp. and E. nivea maintained physical contact with the mutualistic fungus, though no specialised structures related to mycoparasitism were observed. These results showed that Escovopsis is a destructive mycoparasite that needs physical contact for the death of the mutualistic fungus to occur. Also, our findings suggest that E. nivea is an antagonist of the ant fungal cultivar. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-04-01 2018-12-11T16:45:16Z 2018-12-11T16:45:16Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s10482-016-0826-y Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology, v. 110, n. 4, p. 593-605, 2017. 1572-9699 0003-6072 http://hdl.handle.net/11449/169300 10.1007/s10482-016-0826-y 2-s2.0-85007416077 2-s2.0-85007416077.pdf |
url |
http://dx.doi.org/10.1007/s10482-016-0826-y http://hdl.handle.net/11449/169300 |
identifier_str_mv |
Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology, v. 110, n. 4, p. 593-605, 2017. 1572-9699 0003-6072 10.1007/s10482-016-0826-y 2-s2.0-85007416077 2-s2.0-85007416077.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology 0,834 0,834 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
593-605 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808128407877189632 |