Prevalence of Ehrlichia canis (Rickettsiales: Ehrlichieae) DNA in Tissues from Rhipicephalus sanguineus (Acari: Ixodidae) ticks in areas endemic for canine monocytic ehrlichiosis in Brazil
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1093/jme/tjy220 http://hdl.handle.net/11449/190292 |
Resumo: | Canine monocytic ehrlichiosis (CME) is a disease caused by the obligate intracellular bacterium Ehrlichia canis. Tropical lineages of Rhipicephalus sanguineus ticks play an essential role in the transmission of this pathogen. The aim of the present study was to evaluate the prevalence of E. canis DNA in tissue from R. sanguineus ticks in areas endemic for CME in Brazil and quantify levels of E. canis DNA in dissected tissues from these samples. A total of 720 ticks were collected from 72 dogs (36 dogs from the city Araçatuba in São Paulo state and 36 from Campo Grande in the state of Mato Grosso do Sul). Ticks were dissected to collect the guts, ovaries and salivary gland. A quantitative polymerase chain reaction (qPCR) targeting the disulphide bond formation (dsb) protein gene was performed to quantify the level of E. canis infection. The E. canis dsb-qPCR assay was positive for 31.9, 10, and 15.2% of the gut, ovary, and salivary glands, respectively. The average gut, ovary, and salivary gland bacterial load estimated by qPCR was 1.21 × 103, 2.60 × 103, and 4.92 × 103 gene copies/µl, respectively. This is the first report of E. canis DNA in ovaries of R. sanguineus ticks parasitizing dogs in these CME-endemic areas. These observations raise the possibility of E. canis trans-ovarial transmission. |
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Prevalence of Ehrlichia canis (Rickettsiales: Ehrlichieae) DNA in Tissues from Rhipicephalus sanguineus (Acari: Ixodidae) ticks in areas endemic for canine monocytic ehrlichiosis in BrazilEhrlichiosisTickTissueCanine monocytic ehrlichiosis (CME) is a disease caused by the obligate intracellular bacterium Ehrlichia canis. Tropical lineages of Rhipicephalus sanguineus ticks play an essential role in the transmission of this pathogen. The aim of the present study was to evaluate the prevalence of E. canis DNA in tissue from R. sanguineus ticks in areas endemic for CME in Brazil and quantify levels of E. canis DNA in dissected tissues from these samples. A total of 720 ticks were collected from 72 dogs (36 dogs from the city Araçatuba in São Paulo state and 36 from Campo Grande in the state of Mato Grosso do Sul). Ticks were dissected to collect the guts, ovaries and salivary gland. A quantitative polymerase chain reaction (qPCR) targeting the disulphide bond formation (dsb) protein gene was performed to quantify the level of E. canis infection. The E. canis dsb-qPCR assay was positive for 31.9, 10, and 15.2% of the gut, ovary, and salivary glands, respectively. The average gut, ovary, and salivary gland bacterial load estimated by qPCR was 1.21 × 103, 2.60 × 103, and 4.92 × 103 gene copies/µl, respectively. This is the first report of E. canis DNA in ovaries of R. sanguineus ticks parasitizing dogs in these CME-endemic areas. These observations raise the possibility of E. canis trans-ovarial transmission.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Universidade Estadual Paulista (Unesp) Faculdade de Medicina Veterinária de Araçatuba, Clóvis Pestana St., 793 - DonaUniversidade Estadual Paulista Júlio de Mesquita Filho (UNESP) Faculdade de Ciências Agrárias e Veterinárias de Jaboticabal, Via de Acesso Prof. Paulo Donato CastelaneUniversity of Campinas (UNICAMP) Cidade Universitária Zeferino Vaz, Barão GeraldoUnited States Department of Agriculture Agricultural Research Service Knipling-Bushland U. S. Livestock Insects Research Laboratory, 2700, Fredericksburg RoadUniversidade Estadual Paulista (Unesp) Faculdade de Medicina Veterinária de Araçatuba, Clóvis Pestana St., 793 - DonaUniversidade Estadual Paulista Júlio de Mesquita Filho (UNESP) Faculdade de Ciências Agrárias e Veterinárias de Jaboticabal, Via de Acesso Prof. Paulo Donato CastelaneFAPESP: 2014/26461-7Universidade Estadual Paulista (Unesp)Universidade Estadual de Campinas (UNICAMP)Knipling-Bushland U. S. Livestock Insects Research LaboratoryOliveira, Bruno César Miranda [UNESP]Ferrari, Elis Domingos [UNESP]Viol, Milena Araúz [UNESP]André, Marcos Rogério [UNESP]Machado, Rosangela Zacarias [UNESP]De Aquino, Monally Conceição Costa [UNESP]Inácio, Sandra Valéria [UNESP]Gomes, Jancarlo FerreiraGuerrero, Felix D.Bresciani, Katia Denise Saraiva [UNESP]2019-10-06T17:08:27Z2019-10-06T17:08:27Z2019-05-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article828-831http://dx.doi.org/10.1093/jme/tjy220Journal of Medical Entomology, v. 56, n. 3, p. 828-831, 2019.1938-29280022-2585http://hdl.handle.net/11449/19029210.1093/jme/tjy2202-s2.0-85064979674Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Medical Entomologyinfo:eu-repo/semantics/openAccess2024-09-04T19:15:52Zoai:repositorio.unesp.br:11449/190292Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-04T19:15:52Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Prevalence of Ehrlichia canis (Rickettsiales: Ehrlichieae) DNA in Tissues from Rhipicephalus sanguineus (Acari: Ixodidae) ticks in areas endemic for canine monocytic ehrlichiosis in Brazil |
title |
Prevalence of Ehrlichia canis (Rickettsiales: Ehrlichieae) DNA in Tissues from Rhipicephalus sanguineus (Acari: Ixodidae) ticks in areas endemic for canine monocytic ehrlichiosis in Brazil |
spellingShingle |
Prevalence of Ehrlichia canis (Rickettsiales: Ehrlichieae) DNA in Tissues from Rhipicephalus sanguineus (Acari: Ixodidae) ticks in areas endemic for canine monocytic ehrlichiosis in Brazil Oliveira, Bruno César Miranda [UNESP] Ehrlichiosis Tick Tissue |
title_short |
Prevalence of Ehrlichia canis (Rickettsiales: Ehrlichieae) DNA in Tissues from Rhipicephalus sanguineus (Acari: Ixodidae) ticks in areas endemic for canine monocytic ehrlichiosis in Brazil |
title_full |
Prevalence of Ehrlichia canis (Rickettsiales: Ehrlichieae) DNA in Tissues from Rhipicephalus sanguineus (Acari: Ixodidae) ticks in areas endemic for canine monocytic ehrlichiosis in Brazil |
title_fullStr |
Prevalence of Ehrlichia canis (Rickettsiales: Ehrlichieae) DNA in Tissues from Rhipicephalus sanguineus (Acari: Ixodidae) ticks in areas endemic for canine monocytic ehrlichiosis in Brazil |
title_full_unstemmed |
Prevalence of Ehrlichia canis (Rickettsiales: Ehrlichieae) DNA in Tissues from Rhipicephalus sanguineus (Acari: Ixodidae) ticks in areas endemic for canine monocytic ehrlichiosis in Brazil |
title_sort |
Prevalence of Ehrlichia canis (Rickettsiales: Ehrlichieae) DNA in Tissues from Rhipicephalus sanguineus (Acari: Ixodidae) ticks in areas endemic for canine monocytic ehrlichiosis in Brazil |
author |
Oliveira, Bruno César Miranda [UNESP] |
author_facet |
Oliveira, Bruno César Miranda [UNESP] Ferrari, Elis Domingos [UNESP] Viol, Milena Araúz [UNESP] André, Marcos Rogério [UNESP] Machado, Rosangela Zacarias [UNESP] De Aquino, Monally Conceição Costa [UNESP] Inácio, Sandra Valéria [UNESP] Gomes, Jancarlo Ferreira Guerrero, Felix D. Bresciani, Katia Denise Saraiva [UNESP] |
author_role |
author |
author2 |
Ferrari, Elis Domingos [UNESP] Viol, Milena Araúz [UNESP] André, Marcos Rogério [UNESP] Machado, Rosangela Zacarias [UNESP] De Aquino, Monally Conceição Costa [UNESP] Inácio, Sandra Valéria [UNESP] Gomes, Jancarlo Ferreira Guerrero, Felix D. Bresciani, Katia Denise Saraiva [UNESP] |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade Estadual de Campinas (UNICAMP) Knipling-Bushland U. S. Livestock Insects Research Laboratory |
dc.contributor.author.fl_str_mv |
Oliveira, Bruno César Miranda [UNESP] Ferrari, Elis Domingos [UNESP] Viol, Milena Araúz [UNESP] André, Marcos Rogério [UNESP] Machado, Rosangela Zacarias [UNESP] De Aquino, Monally Conceição Costa [UNESP] Inácio, Sandra Valéria [UNESP] Gomes, Jancarlo Ferreira Guerrero, Felix D. Bresciani, Katia Denise Saraiva [UNESP] |
dc.subject.por.fl_str_mv |
Ehrlichiosis Tick Tissue |
topic |
Ehrlichiosis Tick Tissue |
description |
Canine monocytic ehrlichiosis (CME) is a disease caused by the obligate intracellular bacterium Ehrlichia canis. Tropical lineages of Rhipicephalus sanguineus ticks play an essential role in the transmission of this pathogen. The aim of the present study was to evaluate the prevalence of E. canis DNA in tissue from R. sanguineus ticks in areas endemic for CME in Brazil and quantify levels of E. canis DNA in dissected tissues from these samples. A total of 720 ticks were collected from 72 dogs (36 dogs from the city Araçatuba in São Paulo state and 36 from Campo Grande in the state of Mato Grosso do Sul). Ticks were dissected to collect the guts, ovaries and salivary gland. A quantitative polymerase chain reaction (qPCR) targeting the disulphide bond formation (dsb) protein gene was performed to quantify the level of E. canis infection. The E. canis dsb-qPCR assay was positive for 31.9, 10, and 15.2% of the gut, ovary, and salivary glands, respectively. The average gut, ovary, and salivary gland bacterial load estimated by qPCR was 1.21 × 103, 2.60 × 103, and 4.92 × 103 gene copies/µl, respectively. This is the first report of E. canis DNA in ovaries of R. sanguineus ticks parasitizing dogs in these CME-endemic areas. These observations raise the possibility of E. canis trans-ovarial transmission. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-06T17:08:27Z 2019-10-06T17:08:27Z 2019-05-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1093/jme/tjy220 Journal of Medical Entomology, v. 56, n. 3, p. 828-831, 2019. 1938-2928 0022-2585 http://hdl.handle.net/11449/190292 10.1093/jme/tjy220 2-s2.0-85064979674 |
url |
http://dx.doi.org/10.1093/jme/tjy220 http://hdl.handle.net/11449/190292 |
identifier_str_mv |
Journal of Medical Entomology, v. 56, n. 3, p. 828-831, 2019. 1938-2928 0022-2585 10.1093/jme/tjy220 2-s2.0-85064979674 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Medical Entomology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
828-831 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
_version_ |
1810021405723459584 |