Performance characteristics of high performance liquid chromatography, first order derivative UV spectrophotometry and bioassay for fluconazole determination in capsules

Detalhes bibliográficos
Autor(a) principal: Corrêa, Josilene Chaves Ruela [UNESP]
Data de Publicação: 2012
Outros Autores: Chin, Chung Man [UNESP], Salgado, Hérida Regina Nunes [UNESP], Vianna-Soares, Cristina Duarte
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/S0100-40422012000300017
http://hdl.handle.net/11449/8032
Resumo: The bioassay, first order derivative UV spectrophotometry and chromatographic methods for assaying fluconazole capsules were compared. They have shown great advantages over the earlier published methods. Using the first order derivative, the UV spectrophotometry method does not suffer interference of excipients. Validation parameters such as linearity, precision, accuracy, limit of detection and limit of quantitation were determined. All methods were linear and reliable within acceptable limits for antibiotic pharmaceutical preparations being accurate, precise and reproducible. The application of each method as a routine analysis should be investigated considering cost, simplicity, equipment, solvents, speed, and application to large or small workloads.
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spelling Performance characteristics of high performance liquid chromatography, first order derivative UV spectrophotometry and bioassay for fluconazole determination in capsulesfluconazoleliquid chromatographybioassayThe bioassay, first order derivative UV spectrophotometry and chromatographic methods for assaying fluconazole capsules were compared. They have shown great advantages over the earlier published methods. Using the first order derivative, the UV spectrophotometry method does not suffer interference of excipients. Validation parameters such as linearity, precision, accuracy, limit of detection and limit of quantitation were determined. All methods were linear and reliable within acceptable limits for antibiotic pharmaceutical preparations being accurate, precise and reproducible. The application of each method as a routine analysis should be investigated considering cost, simplicity, equipment, solvents, speed, and application to large or small workloads.Programa de Apoio ao Desenvolvimento Científico da Faculdade de Ciências Farmacêuticas da UNESP (PADC)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Universidade Estadual Paulista Faculdade de Ciências Farmacêuticas Departamento de Fármacos e medicamentosUniversidade Federal de Minas Gerais Faculdade de Farmácia Departamento de Produtos FarmacêuticosUniversidade Estadual Paulista Faculdade de Ciências Farmacêuticas Departamento de Fármacos e medicamentosSociedade Brasileira de QuímicaUniversidade Estadual Paulista (Unesp)Universidade Federal de Minas Gerais (UFMG)Corrêa, Josilene Chaves Ruela [UNESP]Chin, Chung Man [UNESP]Salgado, Hérida Regina Nunes [UNESP]Vianna-Soares, Cristina Duarte2014-05-20T13:25:23Z2014-05-20T13:25:23Z2012-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article530-534application/pdfhttp://dx.doi.org/10.1590/S0100-40422012000300017Química Nova. Sociedade Brasileira de Química, v. 35, n. 3, p. 530-534, 2012.0100-4042http://hdl.handle.net/11449/803210.1590/S0100-40422012000300017S0100-40422012000300017WOS:000303071700017S0100-40422012000300017.pdf97343336079754130000-0003-4141-0455SciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengQuímica Nova0.6460,255info:eu-repo/semantics/openAccess2024-06-24T13:45:28Zoai:repositorio.unesp.br:11449/8032Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:59:29.836776Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Performance characteristics of high performance liquid chromatography, first order derivative UV spectrophotometry and bioassay for fluconazole determination in capsules
title Performance characteristics of high performance liquid chromatography, first order derivative UV spectrophotometry and bioassay for fluconazole determination in capsules
spellingShingle Performance characteristics of high performance liquid chromatography, first order derivative UV spectrophotometry and bioassay for fluconazole determination in capsules
Corrêa, Josilene Chaves Ruela [UNESP]
fluconazole
liquid chromatography
bioassay
title_short Performance characteristics of high performance liquid chromatography, first order derivative UV spectrophotometry and bioassay for fluconazole determination in capsules
title_full Performance characteristics of high performance liquid chromatography, first order derivative UV spectrophotometry and bioassay for fluconazole determination in capsules
title_fullStr Performance characteristics of high performance liquid chromatography, first order derivative UV spectrophotometry and bioassay for fluconazole determination in capsules
title_full_unstemmed Performance characteristics of high performance liquid chromatography, first order derivative UV spectrophotometry and bioassay for fluconazole determination in capsules
title_sort Performance characteristics of high performance liquid chromatography, first order derivative UV spectrophotometry and bioassay for fluconazole determination in capsules
author Corrêa, Josilene Chaves Ruela [UNESP]
author_facet Corrêa, Josilene Chaves Ruela [UNESP]
Chin, Chung Man [UNESP]
Salgado, Hérida Regina Nunes [UNESP]
Vianna-Soares, Cristina Duarte
author_role author
author2 Chin, Chung Man [UNESP]
Salgado, Hérida Regina Nunes [UNESP]
Vianna-Soares, Cristina Duarte
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Universidade Federal de Minas Gerais (UFMG)
dc.contributor.author.fl_str_mv Corrêa, Josilene Chaves Ruela [UNESP]
Chin, Chung Man [UNESP]
Salgado, Hérida Regina Nunes [UNESP]
Vianna-Soares, Cristina Duarte
dc.subject.por.fl_str_mv fluconazole
liquid chromatography
bioassay
topic fluconazole
liquid chromatography
bioassay
description The bioassay, first order derivative UV spectrophotometry and chromatographic methods for assaying fluconazole capsules were compared. They have shown great advantages over the earlier published methods. Using the first order derivative, the UV spectrophotometry method does not suffer interference of excipients. Validation parameters such as linearity, precision, accuracy, limit of detection and limit of quantitation were determined. All methods were linear and reliable within acceptable limits for antibiotic pharmaceutical preparations being accurate, precise and reproducible. The application of each method as a routine analysis should be investigated considering cost, simplicity, equipment, solvents, speed, and application to large or small workloads.
publishDate 2012
dc.date.none.fl_str_mv 2012-01-01
2014-05-20T13:25:23Z
2014-05-20T13:25:23Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S0100-40422012000300017
Química Nova. Sociedade Brasileira de Química, v. 35, n. 3, p. 530-534, 2012.
0100-4042
http://hdl.handle.net/11449/8032
10.1590/S0100-40422012000300017
S0100-40422012000300017
WOS:000303071700017
S0100-40422012000300017.pdf
9734333607975413
0000-0003-4141-0455
url http://dx.doi.org/10.1590/S0100-40422012000300017
http://hdl.handle.net/11449/8032
identifier_str_mv Química Nova. Sociedade Brasileira de Química, v. 35, n. 3, p. 530-534, 2012.
0100-4042
10.1590/S0100-40422012000300017
S0100-40422012000300017
WOS:000303071700017
S0100-40422012000300017.pdf
9734333607975413
0000-0003-4141-0455
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Química Nova
0.646
0,255
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 530-534
application/pdf
dc.publisher.none.fl_str_mv Sociedade Brasileira de Química
publisher.none.fl_str_mv Sociedade Brasileira de Química
dc.source.none.fl_str_mv SciELO
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808128590304247808

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