Progesterone dose during synchronization treatment alters luteinizing hormone receptor and steroidogenic enzyme mRNA abundances in granulosa cells of Nellore heifers

Detalhes bibliográficos
Autor(a) principal: Dias, H. P. [UNESP]
Data de Publicação: 2021
Outros Autores: Poole, R. K., Albuquerque, J. P. [UNESP], Santos, P. H. dos [UNESP], Castilho, A. C. S., Pohler, K. G., Vasconcelos, J. L. M. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.anireprosci.2020.106681
http://hdl.handle.net/11449/209952
Resumo: The objective was to investigate effects of progesterone (P-4) dose on abundance of luteinizing hormone receptor (LHCGR), aromatase (CYP19A1), 3 beta-hydroxysteroid dehydrogenase (HSD3B1), and other steroidogenic mRNA transcripts in granulosa cells from dominant follicles. Nellore heifers were assigned to one of six groups: new, first-use controlled internal drug release device (CIDR1) inserted for 5 days (Large-P-4-dose-D5; n = 7) or 6 days (Large-P-4-dose-D6; n = 8), prostaglandin (PG)F-2 alpha administered on DO and 1 previously-used CIDR (CIDR3) inserted for 5 days (Small-P-4-dose-D5; n = 8) or 6 days (Small-P-4-dose-D6; n = 8), CIDR1 inserted on DO and removed plus PGF(2 alpha) on D5 (Large-P-4-dose-pmestrus (PE); n = 7), and CIDR3 and PGF(2 alpha) on DO and 1, CIDR3 removed plus PGF(2 alpha) on D5 (Small-P-4-dose-PE; n = 7). Duration of P-4 treatment (D5 compared to D6) affected abundances of CYP19A1 mRNA transcripts, with there being greater abundances on D6 than D5 (P <= 0.05). Heifers treated with the large dose of P-4 had a smaller dominant follicle, less serum and intra-follicular estradiol (E-2) concentrations (P <= 0.05) and lesser LHCGR, CYP19A1, and HSD3B1 transcript abundances (P <= 0.05). Heifers treated to induce PE had a larger follicle diameter (P = 0.09), greater intra-follicular E-2 concentrations and larger abundances of CYP19A1 mRNA transcript (P <= 0.05) than heifers of the D6 group. Overall, treatment with larger doses of P-4 resulted in lesser abundances of LHCGR, HSD3B1, and CYP19A1 mRNA transcripts; thus, potentially leading to development of smaller dominant follicles and lesser E-2 concentrations.
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spelling Progesterone dose during synchronization treatment alters luteinizing hormone receptor and steroidogenic enzyme mRNA abundances in granulosa cells of Nellore heifersLH receptorProgesteroneGranulosa cellNellore heifersThe objective was to investigate effects of progesterone (P-4) dose on abundance of luteinizing hormone receptor (LHCGR), aromatase (CYP19A1), 3 beta-hydroxysteroid dehydrogenase (HSD3B1), and other steroidogenic mRNA transcripts in granulosa cells from dominant follicles. Nellore heifers were assigned to one of six groups: new, first-use controlled internal drug release device (CIDR1) inserted for 5 days (Large-P-4-dose-D5; n = 7) or 6 days (Large-P-4-dose-D6; n = 8), prostaglandin (PG)F-2 alpha administered on DO and 1 previously-used CIDR (CIDR3) inserted for 5 days (Small-P-4-dose-D5; n = 8) or 6 days (Small-P-4-dose-D6; n = 8), CIDR1 inserted on DO and removed plus PGF(2 alpha) on D5 (Large-P-4-dose-pmestrus (PE); n = 7), and CIDR3 and PGF(2 alpha) on DO and 1, CIDR3 removed plus PGF(2 alpha) on D5 (Small-P-4-dose-PE; n = 7). Duration of P-4 treatment (D5 compared to D6) affected abundances of CYP19A1 mRNA transcripts, with there being greater abundances on D6 than D5 (P <= 0.05). Heifers treated with the large dose of P-4 had a smaller dominant follicle, less serum and intra-follicular estradiol (E-2) concentrations (P <= 0.05) and lesser LHCGR, CYP19A1, and HSD3B1 transcript abundances (P <= 0.05). Heifers treated to induce PE had a larger follicle diameter (P = 0.09), greater intra-follicular E-2 concentrations and larger abundances of CYP19A1 mRNA transcript (P <= 0.05) than heifers of the D6 group. Overall, treatment with larger doses of P-4 resulted in lesser abundances of LHCGR, HSD3B1, and CYP19A1 mRNA transcripts; thus, potentially leading to development of smaller dominant follicles and lesser E-2 concentrations.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)USDA-NIFAHatch/Multistate ProjectSao Paulo State Univ, Dept Anim Prod, BR-18168000 Botucatu, SP, BrazilTexas A&M Univ, Dept Anim Sci, Pregnancy & Dev Programming Area Excellence, College Stn, TX 77843 USASao Paulo State Univ, Inst Biosci, BR-18168000 Botucatu, SP, BrazilUniv Western Sao Paulo, Presidente Prudente, BrazilSao Paulo State Univ, Dept Anim Prod, BR-18168000 Botucatu, SP, BrazilSao Paulo State Univ, Inst Biosci, BR-18168000 Botucatu, SP, BrazilFAPESP: 2013/17181-8USDA-NIFAHatch/Multistate Project: W3112-TEX07702Elsevier B.V.Universidade Estadual Paulista (Unesp)Texas A&M UnivUniv Western Sao PauloDias, H. P. [UNESP]Poole, R. K.Albuquerque, J. P. [UNESP]Santos, P. H. dos [UNESP]Castilho, A. C. S.Pohler, K. G.Vasconcelos, J. L. M. [UNESP]2021-06-25T12:34:43Z2021-06-25T12:34:43Z2021-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10http://dx.doi.org/10.1016/j.anireprosci.2020.106681Animal Reproduction Science. Amsterdam: Elsevier, v. 225, 10 p., 2021.0378-4320http://hdl.handle.net/11449/20995210.1016/j.anireprosci.2020.106681WOS:000610778500007Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAnimal Reproduction Scienceinfo:eu-repo/semantics/openAccess2021-10-23T19:50:11Zoai:repositorio.unesp.br:11449/209952Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T19:50:11Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Progesterone dose during synchronization treatment alters luteinizing hormone receptor and steroidogenic enzyme mRNA abundances in granulosa cells of Nellore heifers
title Progesterone dose during synchronization treatment alters luteinizing hormone receptor and steroidogenic enzyme mRNA abundances in granulosa cells of Nellore heifers
spellingShingle Progesterone dose during synchronization treatment alters luteinizing hormone receptor and steroidogenic enzyme mRNA abundances in granulosa cells of Nellore heifers
Dias, H. P. [UNESP]
LH receptor
Progesterone
Granulosa cell
Nellore heifers
title_short Progesterone dose during synchronization treatment alters luteinizing hormone receptor and steroidogenic enzyme mRNA abundances in granulosa cells of Nellore heifers
title_full Progesterone dose during synchronization treatment alters luteinizing hormone receptor and steroidogenic enzyme mRNA abundances in granulosa cells of Nellore heifers
title_fullStr Progesterone dose during synchronization treatment alters luteinizing hormone receptor and steroidogenic enzyme mRNA abundances in granulosa cells of Nellore heifers
title_full_unstemmed Progesterone dose during synchronization treatment alters luteinizing hormone receptor and steroidogenic enzyme mRNA abundances in granulosa cells of Nellore heifers
title_sort Progesterone dose during synchronization treatment alters luteinizing hormone receptor and steroidogenic enzyme mRNA abundances in granulosa cells of Nellore heifers
author Dias, H. P. [UNESP]
author_facet Dias, H. P. [UNESP]
Poole, R. K.
Albuquerque, J. P. [UNESP]
Santos, P. H. dos [UNESP]
Castilho, A. C. S.
Pohler, K. G.
Vasconcelos, J. L. M. [UNESP]
author_role author
author2 Poole, R. K.
Albuquerque, J. P. [UNESP]
Santos, P. H. dos [UNESP]
Castilho, A. C. S.
Pohler, K. G.
Vasconcelos, J. L. M. [UNESP]
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Texas A&M Univ
Univ Western Sao Paulo
dc.contributor.author.fl_str_mv Dias, H. P. [UNESP]
Poole, R. K.
Albuquerque, J. P. [UNESP]
Santos, P. H. dos [UNESP]
Castilho, A. C. S.
Pohler, K. G.
Vasconcelos, J. L. M. [UNESP]
dc.subject.por.fl_str_mv LH receptor
Progesterone
Granulosa cell
Nellore heifers
topic LH receptor
Progesterone
Granulosa cell
Nellore heifers
description The objective was to investigate effects of progesterone (P-4) dose on abundance of luteinizing hormone receptor (LHCGR), aromatase (CYP19A1), 3 beta-hydroxysteroid dehydrogenase (HSD3B1), and other steroidogenic mRNA transcripts in granulosa cells from dominant follicles. Nellore heifers were assigned to one of six groups: new, first-use controlled internal drug release device (CIDR1) inserted for 5 days (Large-P-4-dose-D5; n = 7) or 6 days (Large-P-4-dose-D6; n = 8), prostaglandin (PG)F-2 alpha administered on DO and 1 previously-used CIDR (CIDR3) inserted for 5 days (Small-P-4-dose-D5; n = 8) or 6 days (Small-P-4-dose-D6; n = 8), CIDR1 inserted on DO and removed plus PGF(2 alpha) on D5 (Large-P-4-dose-pmestrus (PE); n = 7), and CIDR3 and PGF(2 alpha) on DO and 1, CIDR3 removed plus PGF(2 alpha) on D5 (Small-P-4-dose-PE; n = 7). Duration of P-4 treatment (D5 compared to D6) affected abundances of CYP19A1 mRNA transcripts, with there being greater abundances on D6 than D5 (P <= 0.05). Heifers treated with the large dose of P-4 had a smaller dominant follicle, less serum and intra-follicular estradiol (E-2) concentrations (P <= 0.05) and lesser LHCGR, CYP19A1, and HSD3B1 transcript abundances (P <= 0.05). Heifers treated to induce PE had a larger follicle diameter (P = 0.09), greater intra-follicular E-2 concentrations and larger abundances of CYP19A1 mRNA transcript (P <= 0.05) than heifers of the D6 group. Overall, treatment with larger doses of P-4 resulted in lesser abundances of LHCGR, HSD3B1, and CYP19A1 mRNA transcripts; thus, potentially leading to development of smaller dominant follicles and lesser E-2 concentrations.
publishDate 2021
dc.date.none.fl_str_mv 2021-06-25T12:34:43Z
2021-06-25T12:34:43Z
2021-02-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.anireprosci.2020.106681
Animal Reproduction Science. Amsterdam: Elsevier, v. 225, 10 p., 2021.
0378-4320
http://hdl.handle.net/11449/209952
10.1016/j.anireprosci.2020.106681
WOS:000610778500007
url http://dx.doi.org/10.1016/j.anireprosci.2020.106681
http://hdl.handle.net/11449/209952
identifier_str_mv Animal Reproduction Science. Amsterdam: Elsevier, v. 225, 10 p., 2021.
0378-4320
10.1016/j.anireprosci.2020.106681
WOS:000610778500007
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Animal Reproduction Science
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 10
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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