Ruthenium red attenuates brown adipose tissue thermogenesis in rats
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.jtherbio.2020.102779 http://hdl.handle.net/11449/209925 |
Resumo: | Ruthenium red (RR) is a non-selective antagonist of the temperature-sensitive Transient Receptor Potential (TRP) channels and it is an important pharmacological tool in thermoregulatory research. However, the effect of RR on thermoeffector activity is not well established. Here we evaluated the effect of RR on cold-defense thermoeffectors induced by menthol, an agonist of the cold-sensitive TRPM8 channel. Adult male Wistar rats were used. Epidermal treatment with menthol raised deep body temperature due to an increase in oxygen consumption (an index of thermogenesis), a reduction in heat loss index (an index of cutaneous vasoconstriction), and an induction in warmth-seeking behavior in a two-temperature choice apparatus. Pretreatment with RR attenuated the menthol-induced increase in deep body temperature and oxygen consumption, but it did not affect heat loss index and warmth-seeking behavior. To stimulate brown adipose tissue thermogenesis, rats were treated with CL 316,243, a potent and selective beta 3-adrenoceptor agonist. CL 316,243 increased deep body temperature, which was attenuated by RR pretreatment. We conclude that RR reduces brown adipose tissue thermogenesis induced by menthol and CL 316,243, independent of effects at the thermal sensor level (i.e., TRPM8). |
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Ruthenium red attenuates brown adipose tissue thermogenesis in ratsCL 316,243MentholTRPM8ThermoregulationRuthenium red (RR) is a non-selective antagonist of the temperature-sensitive Transient Receptor Potential (TRP) channels and it is an important pharmacological tool in thermoregulatory research. However, the effect of RR on thermoeffector activity is not well established. Here we evaluated the effect of RR on cold-defense thermoeffectors induced by menthol, an agonist of the cold-sensitive TRPM8 channel. Adult male Wistar rats were used. Epidermal treatment with menthol raised deep body temperature due to an increase in oxygen consumption (an index of thermogenesis), a reduction in heat loss index (an index of cutaneous vasoconstriction), and an induction in warmth-seeking behavior in a two-temperature choice apparatus. Pretreatment with RR attenuated the menthol-induced increase in deep body temperature and oxygen consumption, but it did not affect heat loss index and warmth-seeking behavior. To stimulate brown adipose tissue thermogenesis, rats were treated with CL 316,243, a potent and selective beta 3-adrenoceptor agonist. CL 316,243 increased deep body temperature, which was attenuated by RR pretreatment. We conclude that RR reduces brown adipose tissue thermogenesis induced by menthol and CL 316,243, independent of effects at the thermal sensor level (i.e., TRPM8).Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Univ Fed ABC, Grad Program Neurosci & Cognit, Sao Bernardo Do Campo, SP, BrazilUniv Fed ABC, Nat & Humanities Sci Ctr, Rua Arcturus 03, BR-09606 Sao Bernardo Do Campo, SP, BrazilSao Paulo State Univ, Biosci Inst, Dept Zool, Rio Claro, SP, BrazilSao Paulo State Univ, Biosci Inst, Dept Zool, Rio Claro, SP, BrazilCNPq: 449102/2014-9FAPESP: 2015/02991-0FAPESP: 2015/23426-9FAPESP: 2013/25503-5Elsevier B.V.Universidade Federal do ABC (UFABC)Universidade Estadual Paulista (Unesp)Lillo Vizin, Robson CristianoCarrettiero, Daniel CarneiroIshikawa, Debora T.Cruz-Neto, Ariovaldo P. [UNESP]Almeida, Maria Camila2021-06-25T12:33:53Z2021-06-25T12:33:53Z2021-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article7http://dx.doi.org/10.1016/j.jtherbio.2020.102779Journal Of Thermal Biology. Oxford: Pergamon-elsevier Science Ltd, v. 95, 7 p., 2021.0306-4565http://hdl.handle.net/11449/20992510.1016/j.jtherbio.2020.102779WOS:000609163700003Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal Of Thermal Biologyinfo:eu-repo/semantics/openAccess2021-10-23T19:50:11Zoai:repositorio.unesp.br:11449/209925Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T16:55:58.806421Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Ruthenium red attenuates brown adipose tissue thermogenesis in rats |
title |
Ruthenium red attenuates brown adipose tissue thermogenesis in rats |
spellingShingle |
Ruthenium red attenuates brown adipose tissue thermogenesis in rats Lillo Vizin, Robson Cristiano CL 316,243 Menthol TRPM8 Thermoregulation |
title_short |
Ruthenium red attenuates brown adipose tissue thermogenesis in rats |
title_full |
Ruthenium red attenuates brown adipose tissue thermogenesis in rats |
title_fullStr |
Ruthenium red attenuates brown adipose tissue thermogenesis in rats |
title_full_unstemmed |
Ruthenium red attenuates brown adipose tissue thermogenesis in rats |
title_sort |
Ruthenium red attenuates brown adipose tissue thermogenesis in rats |
author |
Lillo Vizin, Robson Cristiano |
author_facet |
Lillo Vizin, Robson Cristiano Carrettiero, Daniel Carneiro Ishikawa, Debora T. Cruz-Neto, Ariovaldo P. [UNESP] Almeida, Maria Camila |
author_role |
author |
author2 |
Carrettiero, Daniel Carneiro Ishikawa, Debora T. Cruz-Neto, Ariovaldo P. [UNESP] Almeida, Maria Camila |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal do ABC (UFABC) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Lillo Vizin, Robson Cristiano Carrettiero, Daniel Carneiro Ishikawa, Debora T. Cruz-Neto, Ariovaldo P. [UNESP] Almeida, Maria Camila |
dc.subject.por.fl_str_mv |
CL 316,243 Menthol TRPM8 Thermoregulation |
topic |
CL 316,243 Menthol TRPM8 Thermoregulation |
description |
Ruthenium red (RR) is a non-selective antagonist of the temperature-sensitive Transient Receptor Potential (TRP) channels and it is an important pharmacological tool in thermoregulatory research. However, the effect of RR on thermoeffector activity is not well established. Here we evaluated the effect of RR on cold-defense thermoeffectors induced by menthol, an agonist of the cold-sensitive TRPM8 channel. Adult male Wistar rats were used. Epidermal treatment with menthol raised deep body temperature due to an increase in oxygen consumption (an index of thermogenesis), a reduction in heat loss index (an index of cutaneous vasoconstriction), and an induction in warmth-seeking behavior in a two-temperature choice apparatus. Pretreatment with RR attenuated the menthol-induced increase in deep body temperature and oxygen consumption, but it did not affect heat loss index and warmth-seeking behavior. To stimulate brown adipose tissue thermogenesis, rats were treated with CL 316,243, a potent and selective beta 3-adrenoceptor agonist. CL 316,243 increased deep body temperature, which was attenuated by RR pretreatment. We conclude that RR reduces brown adipose tissue thermogenesis induced by menthol and CL 316,243, independent of effects at the thermal sensor level (i.e., TRPM8). |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-25T12:33:53Z 2021-06-25T12:33:53Z 2021-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.jtherbio.2020.102779 Journal Of Thermal Biology. Oxford: Pergamon-elsevier Science Ltd, v. 95, 7 p., 2021. 0306-4565 http://hdl.handle.net/11449/209925 10.1016/j.jtherbio.2020.102779 WOS:000609163700003 |
url |
http://dx.doi.org/10.1016/j.jtherbio.2020.102779 http://hdl.handle.net/11449/209925 |
identifier_str_mv |
Journal Of Thermal Biology. Oxford: Pergamon-elsevier Science Ltd, v. 95, 7 p., 2021. 0306-4565 10.1016/j.jtherbio.2020.102779 WOS:000609163700003 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal Of Thermal Biology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
7 |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128723169312768 |