Ruthenium red attenuates brown adipose tissue thermogenesis in rats

Detalhes bibliográficos
Autor(a) principal: Lillo Vizin, Robson Cristiano
Data de Publicação: 2021
Outros Autores: Carrettiero, Daniel Carneiro, Ishikawa, Debora T., Cruz-Neto, Ariovaldo P. [UNESP], Almeida, Maria Camila
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.jtherbio.2020.102779
http://hdl.handle.net/11449/209925
Resumo: Ruthenium red (RR) is a non-selective antagonist of the temperature-sensitive Transient Receptor Potential (TRP) channels and it is an important pharmacological tool in thermoregulatory research. However, the effect of RR on thermoeffector activity is not well established. Here we evaluated the effect of RR on cold-defense thermoeffectors induced by menthol, an agonist of the cold-sensitive TRPM8 channel. Adult male Wistar rats were used. Epidermal treatment with menthol raised deep body temperature due to an increase in oxygen consumption (an index of thermogenesis), a reduction in heat loss index (an index of cutaneous vasoconstriction), and an induction in warmth-seeking behavior in a two-temperature choice apparatus. Pretreatment with RR attenuated the menthol-induced increase in deep body temperature and oxygen consumption, but it did not affect heat loss index and warmth-seeking behavior. To stimulate brown adipose tissue thermogenesis, rats were treated with CL 316,243, a potent and selective beta 3-adrenoceptor agonist. CL 316,243 increased deep body temperature, which was attenuated by RR pretreatment. We conclude that RR reduces brown adipose tissue thermogenesis induced by menthol and CL 316,243, independent of effects at the thermal sensor level (i.e., TRPM8).
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spelling Ruthenium red attenuates brown adipose tissue thermogenesis in ratsCL 316,243MentholTRPM8ThermoregulationRuthenium red (RR) is a non-selective antagonist of the temperature-sensitive Transient Receptor Potential (TRP) channels and it is an important pharmacological tool in thermoregulatory research. However, the effect of RR on thermoeffector activity is not well established. Here we evaluated the effect of RR on cold-defense thermoeffectors induced by menthol, an agonist of the cold-sensitive TRPM8 channel. Adult male Wistar rats were used. Epidermal treatment with menthol raised deep body temperature due to an increase in oxygen consumption (an index of thermogenesis), a reduction in heat loss index (an index of cutaneous vasoconstriction), and an induction in warmth-seeking behavior in a two-temperature choice apparatus. Pretreatment with RR attenuated the menthol-induced increase in deep body temperature and oxygen consumption, but it did not affect heat loss index and warmth-seeking behavior. To stimulate brown adipose tissue thermogenesis, rats were treated with CL 316,243, a potent and selective beta 3-adrenoceptor agonist. CL 316,243 increased deep body temperature, which was attenuated by RR pretreatment. We conclude that RR reduces brown adipose tissue thermogenesis induced by menthol and CL 316,243, independent of effects at the thermal sensor level (i.e., TRPM8).Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Univ Fed ABC, Grad Program Neurosci & Cognit, Sao Bernardo Do Campo, SP, BrazilUniv Fed ABC, Nat & Humanities Sci Ctr, Rua Arcturus 03, BR-09606 Sao Bernardo Do Campo, SP, BrazilSao Paulo State Univ, Biosci Inst, Dept Zool, Rio Claro, SP, BrazilSao Paulo State Univ, Biosci Inst, Dept Zool, Rio Claro, SP, BrazilCNPq: 449102/2014-9FAPESP: 2015/02991-0FAPESP: 2015/23426-9FAPESP: 2013/25503-5Elsevier B.V.Universidade Federal do ABC (UFABC)Universidade Estadual Paulista (Unesp)Lillo Vizin, Robson CristianoCarrettiero, Daniel CarneiroIshikawa, Debora T.Cruz-Neto, Ariovaldo P. [UNESP]Almeida, Maria Camila2021-06-25T12:33:53Z2021-06-25T12:33:53Z2021-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article7http://dx.doi.org/10.1016/j.jtherbio.2020.102779Journal Of Thermal Biology. Oxford: Pergamon-elsevier Science Ltd, v. 95, 7 p., 2021.0306-4565http://hdl.handle.net/11449/20992510.1016/j.jtherbio.2020.102779WOS:000609163700003Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal Of Thermal Biologyinfo:eu-repo/semantics/openAccess2021-10-23T19:50:11Zoai:repositorio.unesp.br:11449/209925Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T16:55:58.806421Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Ruthenium red attenuates brown adipose tissue thermogenesis in rats
title Ruthenium red attenuates brown adipose tissue thermogenesis in rats
spellingShingle Ruthenium red attenuates brown adipose tissue thermogenesis in rats
Lillo Vizin, Robson Cristiano
CL 316,243
Menthol
TRPM8
Thermoregulation
title_short Ruthenium red attenuates brown adipose tissue thermogenesis in rats
title_full Ruthenium red attenuates brown adipose tissue thermogenesis in rats
title_fullStr Ruthenium red attenuates brown adipose tissue thermogenesis in rats
title_full_unstemmed Ruthenium red attenuates brown adipose tissue thermogenesis in rats
title_sort Ruthenium red attenuates brown adipose tissue thermogenesis in rats
author Lillo Vizin, Robson Cristiano
author_facet Lillo Vizin, Robson Cristiano
Carrettiero, Daniel Carneiro
Ishikawa, Debora T.
Cruz-Neto, Ariovaldo P. [UNESP]
Almeida, Maria Camila
author_role author
author2 Carrettiero, Daniel Carneiro
Ishikawa, Debora T.
Cruz-Neto, Ariovaldo P. [UNESP]
Almeida, Maria Camila
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal do ABC (UFABC)
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Lillo Vizin, Robson Cristiano
Carrettiero, Daniel Carneiro
Ishikawa, Debora T.
Cruz-Neto, Ariovaldo P. [UNESP]
Almeida, Maria Camila
dc.subject.por.fl_str_mv CL 316,243
Menthol
TRPM8
Thermoregulation
topic CL 316,243
Menthol
TRPM8
Thermoregulation
description Ruthenium red (RR) is a non-selective antagonist of the temperature-sensitive Transient Receptor Potential (TRP) channels and it is an important pharmacological tool in thermoregulatory research. However, the effect of RR on thermoeffector activity is not well established. Here we evaluated the effect of RR on cold-defense thermoeffectors induced by menthol, an agonist of the cold-sensitive TRPM8 channel. Adult male Wistar rats were used. Epidermal treatment with menthol raised deep body temperature due to an increase in oxygen consumption (an index of thermogenesis), a reduction in heat loss index (an index of cutaneous vasoconstriction), and an induction in warmth-seeking behavior in a two-temperature choice apparatus. Pretreatment with RR attenuated the menthol-induced increase in deep body temperature and oxygen consumption, but it did not affect heat loss index and warmth-seeking behavior. To stimulate brown adipose tissue thermogenesis, rats were treated with CL 316,243, a potent and selective beta 3-adrenoceptor agonist. CL 316,243 increased deep body temperature, which was attenuated by RR pretreatment. We conclude that RR reduces brown adipose tissue thermogenesis induced by menthol and CL 316,243, independent of effects at the thermal sensor level (i.e., TRPM8).
publishDate 2021
dc.date.none.fl_str_mv 2021-06-25T12:33:53Z
2021-06-25T12:33:53Z
2021-01-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.jtherbio.2020.102779
Journal Of Thermal Biology. Oxford: Pergamon-elsevier Science Ltd, v. 95, 7 p., 2021.
0306-4565
http://hdl.handle.net/11449/209925
10.1016/j.jtherbio.2020.102779
WOS:000609163700003
url http://dx.doi.org/10.1016/j.jtherbio.2020.102779
http://hdl.handle.net/11449/209925
identifier_str_mv Journal Of Thermal Biology. Oxford: Pergamon-elsevier Science Ltd, v. 95, 7 p., 2021.
0306-4565
10.1016/j.jtherbio.2020.102779
WOS:000609163700003
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal Of Thermal Biology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 7
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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