Comparison between conventional and chemomechanical approaches for the removal of carious dentin: an in vitro study
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1038/s41598-020-65159-x http://hdl.handle.net/11449/201757 |
Resumo: | The present study aimed to evaluate the efficiency, effectiveness, and biocompatibility of two agents used for the chemomechanical removal of carious dentin. Sixty extracted carious human teeth were treated with a conventional bur (CBG) or chemomechanical agents – Papacarie Duo (PG) and Brix 3000 (BG). Treatment efficiency and effectiveness were assessed by the working time for carious dentin removal and Knoop microhardness values, respectively. Human pulp fibroblasts (FP6) were used to evaluate cytotoxicity by incorporating MTT dye, and genotoxicity was evaluated with the micronuclei test. The carious tissue was removed in a shorter time with CBG (median = 54.0 seconds) than the time required for chemomechanical agents (p = 0.0001). However, the time was shorter for Brix 3000 (BG) than that for Papacarie Duo (PG), showing mean values of 85.0 and 110.5 seconds, respectively. Regarding microhardness testing, all approaches tested were effective (p < 0.05). The final mean microhardness values were 48.54 ± 16.31 KHN, 43.23 ± 13.26 KHN, and 47.63 ± 22.40 KHN for PG, BG, and CBG, respectively. PG decreased cell viability compared to that of BG, but it presented no genotoxicity. Brix 3000 may be a good option for chemomechanical dentin caries removal due to its reduced removal time and lower cytotoxicity compared to the other treatment options. |
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Comparison between conventional and chemomechanical approaches for the removal of carious dentin: an in vitro studyThe present study aimed to evaluate the efficiency, effectiveness, and biocompatibility of two agents used for the chemomechanical removal of carious dentin. Sixty extracted carious human teeth were treated with a conventional bur (CBG) or chemomechanical agents – Papacarie Duo (PG) and Brix 3000 (BG). Treatment efficiency and effectiveness were assessed by the working time for carious dentin removal and Knoop microhardness values, respectively. Human pulp fibroblasts (FP6) were used to evaluate cytotoxicity by incorporating MTT dye, and genotoxicity was evaluated with the micronuclei test. The carious tissue was removed in a shorter time with CBG (median = 54.0 seconds) than the time required for chemomechanical agents (p = 0.0001). However, the time was shorter for Brix 3000 (BG) than that for Papacarie Duo (PG), showing mean values of 85.0 and 110.5 seconds, respectively. Regarding microhardness testing, all approaches tested were effective (p < 0.05). The final mean microhardness values were 48.54 ± 16.31 KHN, 43.23 ± 13.26 KHN, and 47.63 ± 22.40 KHN for PG, BG, and CBG, respectively. PG decreased cell viability compared to that of BG, but it presented no genotoxicity. Brix 3000 may be a good option for chemomechanical dentin caries removal due to its reduced removal time and lower cytotoxicity compared to the other treatment options.Postgraduate Program in Dentistry Federal University of Sergipe (UFS)Department of Restorative Dentistry Institute of Science and Technology São Paulo State University (Unesp)Postgraduate Program in Dentistry School of Dentistry Federal University of Uberlândia (UFU)Department of Restorative Dental Sciences University of Florida (UF)Department of Pediatric Dentistry School of Dentistry Federal University of Uberlândia (UFU)Department of Dentistry School of Dentistry University of Araraquara (UNIARA)Department of Dentistry Paraíba State University (UEPB)Department of Preventive and Social Dentistry School of Dentistry Federal University of Uberlândia (UFU)Department of Restorative Dentistry Institute of Science and Technology São Paulo State University (Unesp)Universidade Federal de Sergipe (UFS)Universidade Estadual Paulista (Unesp)Universidade Federal de Uberlândia (UFU)University of Florida (UF)University of Araraquara (UNIARA)Paraíba State University (UEPB)Santos, Tito Marcel LimaBresciani, Eduardo [UNESP]Matos, Felipe de SouzaCamargo, Samira Esteves AfonsoHidalgo, Ana Paula TurrioniRivera, Luciana Monti LimaBernardino, Ítalo de MacedoParanhos, Luiz Renato2020-12-12T02:41:00Z2020-12-12T02:41:00Z2020-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1038/s41598-020-65159-xScientific Reports, v. 10, n. 1, 2020.2045-2322http://hdl.handle.net/11449/20175710.1038/s41598-020-65159-x2-s2.0-85084787612Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengScientific Reportsinfo:eu-repo/semantics/openAccess2021-10-22T21:16:10Zoai:repositorio.unesp.br:11449/201757Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-22T21:16:10Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Comparison between conventional and chemomechanical approaches for the removal of carious dentin: an in vitro study |
title |
Comparison between conventional and chemomechanical approaches for the removal of carious dentin: an in vitro study |
spellingShingle |
Comparison between conventional and chemomechanical approaches for the removal of carious dentin: an in vitro study Santos, Tito Marcel Lima |
title_short |
Comparison between conventional and chemomechanical approaches for the removal of carious dentin: an in vitro study |
title_full |
Comparison between conventional and chemomechanical approaches for the removal of carious dentin: an in vitro study |
title_fullStr |
Comparison between conventional and chemomechanical approaches for the removal of carious dentin: an in vitro study |
title_full_unstemmed |
Comparison between conventional and chemomechanical approaches for the removal of carious dentin: an in vitro study |
title_sort |
Comparison between conventional and chemomechanical approaches for the removal of carious dentin: an in vitro study |
author |
Santos, Tito Marcel Lima |
author_facet |
Santos, Tito Marcel Lima Bresciani, Eduardo [UNESP] Matos, Felipe de Souza Camargo, Samira Esteves Afonso Hidalgo, Ana Paula Turrioni Rivera, Luciana Monti Lima Bernardino, Ítalo de Macedo Paranhos, Luiz Renato |
author_role |
author |
author2 |
Bresciani, Eduardo [UNESP] Matos, Felipe de Souza Camargo, Samira Esteves Afonso Hidalgo, Ana Paula Turrioni Rivera, Luciana Monti Lima Bernardino, Ítalo de Macedo Paranhos, Luiz Renato |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de Sergipe (UFS) Universidade Estadual Paulista (Unesp) Universidade Federal de Uberlândia (UFU) University of Florida (UF) University of Araraquara (UNIARA) Paraíba State University (UEPB) |
dc.contributor.author.fl_str_mv |
Santos, Tito Marcel Lima Bresciani, Eduardo [UNESP] Matos, Felipe de Souza Camargo, Samira Esteves Afonso Hidalgo, Ana Paula Turrioni Rivera, Luciana Monti Lima Bernardino, Ítalo de Macedo Paranhos, Luiz Renato |
description |
The present study aimed to evaluate the efficiency, effectiveness, and biocompatibility of two agents used for the chemomechanical removal of carious dentin. Sixty extracted carious human teeth were treated with a conventional bur (CBG) or chemomechanical agents – Papacarie Duo (PG) and Brix 3000 (BG). Treatment efficiency and effectiveness were assessed by the working time for carious dentin removal and Knoop microhardness values, respectively. Human pulp fibroblasts (FP6) were used to evaluate cytotoxicity by incorporating MTT dye, and genotoxicity was evaluated with the micronuclei test. The carious tissue was removed in a shorter time with CBG (median = 54.0 seconds) than the time required for chemomechanical agents (p = 0.0001). However, the time was shorter for Brix 3000 (BG) than that for Papacarie Duo (PG), showing mean values of 85.0 and 110.5 seconds, respectively. Regarding microhardness testing, all approaches tested were effective (p < 0.05). The final mean microhardness values were 48.54 ± 16.31 KHN, 43.23 ± 13.26 KHN, and 47.63 ± 22.40 KHN for PG, BG, and CBG, respectively. PG decreased cell viability compared to that of BG, but it presented no genotoxicity. Brix 3000 may be a good option for chemomechanical dentin caries removal due to its reduced removal time and lower cytotoxicity compared to the other treatment options. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T02:41:00Z 2020-12-12T02:41:00Z 2020-12-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1038/s41598-020-65159-x Scientific Reports, v. 10, n. 1, 2020. 2045-2322 http://hdl.handle.net/11449/201757 10.1038/s41598-020-65159-x 2-s2.0-85084787612 |
url |
http://dx.doi.org/10.1038/s41598-020-65159-x http://hdl.handle.net/11449/201757 |
identifier_str_mv |
Scientific Reports, v. 10, n. 1, 2020. 2045-2322 10.1038/s41598-020-65159-x 2-s2.0-85084787612 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Scientific Reports |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1803649634682273792 |